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- EMDB-57253: CryoET of archaellated cell pole of L. aerophila -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-57253
TitleCryoET of archaellated cell pole of L. aerophila
Map data
Sample
  • Cell: CryoET of archaellated cell pole of L. aerophila
KeywordsCell surface / CryoET / cell pole / cell envelope / UNKNOWN FUNCTION / Archaellum
Biological speciesLitorilinea aerophila (bacteria)
Methodelectron tomography / cryo EM
AuthorsJoest M / Sivabalasarma S / Mollat CL / Albers S-V
Funding support Germany, 2 items
OrganizationGrant numberCountry
German Research Foundation (DFG)403222702 Germany
German Research Foundation (DFG)390939984 Germany
CitationJournal: To Be Published
Title: Filamentous growth, cell envelope architecture and surface appendages in Litorilinea aerophila
Authors: Joest M / Mollat CL / Mutschler L / Rodriguez-Franco M / Sivabalasarma S / Drepper F / Huesgen PF / Ott T / Albers S-V
History
DepositionMar 23, 2026-
Header (metadata) releaseJun 24, 2026-
Map releaseJun 24, 2026-
UpdateJun 24, 2026-
Current statusJun 24, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_57253.png

Downloads & links

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Map

FileDownload / File: emd_57253.map.gz / Format: CCP4 / Size: 1.6 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
18.8 Å/pix.
x 400 pix.
= 7520. Å		18.8 Å/pix.
x 1024 pix.
= 19251.199 Å		18.8 Å/pix.
x 1024 pix.
= 19251.199 Å

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 18.8 Å
Density

Histogram

Histogram (log scale)
Minimum - Maximum-11.928526 - 10.243919
Average (Standard dev.)0.0071335738 (±0.7883953)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions10241024400
Spacing10241024400
CellA: 19251.2 Å / B: 19251.2 Å / C: 7519.9995 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : CryoET of archaellated cell pole of L. aerophila

EntireName: CryoET of archaellated cell pole of L. aerophila
Components
  • Cell: CryoET of archaellated cell pole of L. aerophila

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Supramolecule #1: CryoET of archaellated cell pole of L. aerophila

SupramoleculeName: CryoET of archaellated cell pole of L. aerophila / type: cell / ID: 1 / Parent: 0
Source (natural)Organism: Litorilinea aerophila (bacteria)

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Experimental details

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Structure determination

Methodcryo EM
Processingelectron tomography
Aggregation statecell

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE
SectioningOther: NO SECTIONING

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 4.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 8.0 µm / Nominal defocus min: 5.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionSoftware - Name: AreTomo / Number images used: 60
CTF correctionType: NONE

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