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- EMDB-54773: C3 map of the filament formed by the grappling-hook like protein ... -

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Basic information

Entry
Database: EMDB / ID: EMD-54773
TitleC3 map of the filament formed by the grappling-hook like protein A from L. aerophila
Map data
Sample
  • Organelle or cellular component: Purified filaments formed by the grappling-hook like protein A from L. aerophila
KeywordsCell surface structure / grappling-hook like protein A / UNKNOWN FUNCTION
Biological speciesLitorilinea aerophila (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 7.14 Å
AuthorsJoest M / Mollat CL / Sivabalasarma S / Albers S-V
Funding support Germany, 1 items
OrganizationGrant numberCountry
German Research Foundation (DFG)403222702 Germany
CitationJournal: To Be Published
Title: Filamentous growth, cell envelope architecture and surface appendages in Litorilinea aerophila
Authors: Joest M / Mollat CL / Mutschler L / Rodriguez-Franco M / Sivabalasarma S / Drepper F / Huesgen PF / Ott T / Albers S-V
History
DepositionAug 12, 2025-
Header (metadata) releaseJun 24, 2026-
Map releaseJun 24, 2026-
UpdateJun 24, 2026-
Current statusJun 24, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_54773.png

Downloads & links

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Map

FileDownload / File: emd_54773.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.22 Å/pix.
x 300 pix.
= 366. Å		1.22 Å/pix.
x 300 pix.
= 366. Å		1.22 Å/pix.
x 300 pix.
= 366. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.22 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.113
Minimum - Maximum-0.2890267 - 0.46961063
Average (Standard dev.)0.000558959 (±0.015493568)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 366.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_54773_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_54773_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Purified filaments formed by the grappling-hook like protein A fr...

EntireName: Purified filaments formed by the grappling-hook like protein A from L. aerophila
Components
  • Organelle or cellular component: Purified filaments formed by the grappling-hook like protein A from L. aerophila

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Supramolecule #1: Purified filaments formed by the grappling-hook like protein A fr...

SupramoleculeName: Purified filaments formed by the grappling-hook like protein A from L. aerophila
type: organelle_or_cellular_component / ID: 1 / Parent: 0
Source (natural)Organism: Litorilinea aerophila (bacteria)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 7.14 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 24135
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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