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Entry
Database: EMDB / ID: EMD-55878
TitleCryo-EM structure of the inward rectifying potassium channel 7.1 (Kir7.1)
Map dataEM map used for model building and refinement.
Sample
  • Complex: Tetrameric assembly of human inward rectifying potassium channel 7.1
    • Protein or peptide: Inward rectifier potassium channel 13
  • Ligand: POTASSIUM ION
Keywordspotassium ion channel / active conformation / human membrane protein / MEMBRANE PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsO'Malley N / Faust B / Nasrallah C / Wallace BA
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Biotechnology and Biological Sciences Research Council (BBSRC) United Kingdom
CitationJournal: To Be Published
Title: Cryo-EM structure of human Kir7.1 reveals the molecular basis of Snowflake Vitreoretinal Degeneration
Authors: O'Malley N / Nasrallah C / Churchill A / Bertrand J / Faust B / Wallace BA
History
DepositionNov 27, 2025-
Header (metadata) releaseJul 1, 2026-
Map releaseJul 1, 2026-
UpdateJul 1, 2026-
Current statusJul 1, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_55878.png

Downloads & links

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Map

FileReleased
AnnotationEM map used for model building and refinement.
Voxel sizeX=Y=Z: 0.65 Å
Density
Contour LevelBy AUTHOR: 0.122
Minimum - Maximum-1.646372 - 1.9794393
Average (Standard dev.)-0.00015121615 (±0.026551357)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions440440440
Spacing440440440
CellA=B=C: 286.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Tetrameric assembly of human inward rectifying potassium channel 7.1

EntireName: Tetrameric assembly of human inward rectifying potassium channel 7.1
Components
  • Complex: Tetrameric assembly of human inward rectifying potassium channel 7.1
    • Protein or peptide: Inward rectifier potassium channel 13
  • Ligand: POTASSIUM ION

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Supramolecule #1: Tetrameric assembly of human inward rectifying potassium channel 7.1

SupramoleculeName: Tetrameric assembly of human inward rectifying potassium channel 7.1
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Inward rectifier potassium channel 13

MacromoleculeName: Inward rectifier potassium channel 13 / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 41.710543 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: DYKDDDDKGS MDSSNCKVIA PLLSQRYRRM VTKDGHSTLQ MDGAQRGLAY LRDAWGILMD MRWRWMMLVF SASFVVHWLV FAVLWYVLA EMNGDLELDH DAPPENHTIC VKYITSFTAA FSFSLETQLT IGYGTMFPSG DCPSAIALLA IQMLLGLMLE A FITGAFVA ...String:
DYKDDDDKGS MDSSNCKVIA PLLSQRYRRM VTKDGHSTLQ MDGAQRGLAY LRDAWGILMD MRWRWMMLVF SASFVVHWLV FAVLWYVLA EMNGDLELDH DAPPENHTIC VKYITSFTAA FSFSLETQLT IGYGTMFPSG DCPSAIALLA IQMLLGLMLE A FITGAFVA KIARPKNRAF SIRFTDTAVV AHMDGKPNLI FQVANTRPSP LTSVRVSAVL YQERENGKLY QTSVDFHLDG IS SDECPFF IFPLTYYHSI TPSSPLATLL QHENPSHFEL VVFLSAMQEG TGEICQRRTS YLPSEIMLHH CFASLLTRGS KGE YQIKME NFDKTVPEFP TPLVSKSPNR TDLDIHINGQ SIDNFQISET GLTE

UniProtKB: Inward rectifier potassium channel 13

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Macromolecule #2: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 2 / Number of copies: 2 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration6 mg/mL
BufferpH: 7.5
GridModel: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 300
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number real images: 22098 / Average electron dose: 31.9 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.7 µm / Nominal defocus min: 1.2 µm
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 5871876
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL / In silico model: ModelAngelo produced the initial model.
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C4 (4 fold cyclic) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.2) / Number images used: 382328
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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Atomic model buiding 1

Initial modelChain - Source name: Other / Chain - Initial model type: in silico model / Details: ModelAngelo
RefinementProtocol: AB INITIO MODEL
Output model

PDB-9tfv:
Cryo-EM structure of the inward rectifying potassium channel 7.1 (Kir7.1)

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