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- EMDB-55594: Chlorophyll f-containing monomeric far-red Photosystem II from Ca... -

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Basic information

Entry
Database: EMDB / ID: EMD-55594
TitleChlorophyll f-containing monomeric far-red Photosystem II from Calothrix sp. NIES-3974
Map data
Sample
  • Complex: Chlorophyll f-containing monomeric far-red Photosystem II
    • Protein or peptide: x 17 types
  • Ligand: x 17 types
KeywordsChlorophyll f / photosystem II / ELECTRON TRANSPORT / FaRLiP
Biological speciesCalothrix sp. NIES-3974 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.33 Å
AuthorsLeong HF / Consoli G / Murray JW / Fantuzzi A / Rutherford AW
Funding support United Kingdom, European Union, 11 items
OrganizationGrant numberCountry
Biotechnology and Biological Sciences Research Council (BBSRC)BB/X019284/1 United Kingdom
H2020 Marie Curie Actions of the European Commission955520European Union
Biotechnology and Biological Sciences Research Council (BBSRC)BB/R001383/1 United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)BB/V002015/1 United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)BB/R00921X United Kingdom
Leverhulme TrustRPG-2022-203 United Kingdom
Royal SocietyRSRP/S2/242010 United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)BB/M011178/1 United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)BB/Z516740/1 United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)UKRI2820 United Kingdom
Imperial College LondonPresidents PhD Scholarship United Kingdom
CitationJournal: Nat Commun / Year: 2026
Title: Mapping the absorption landscape of far-red Photosystem II
Authors: Leong HF / Consoli G / Davis GA / Hancox-Lachman B / Renard K / Tufail F / Lee LE / Gautier L / Murray JW / Fantuzzi A / Rutherford AW
History
DepositionNov 6, 2025-
Header (metadata) releaseJun 10, 2026-
Map releaseJun 10, 2026-
UpdateJun 10, 2026-
Current statusJun 10, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_55594.png

Downloads & links

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Map

FileDownload / File: emd_55594.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.83 Å/pix.
x 600 pix.
= 496.2 Å		0.83 Å/pix.
x 600 pix.
= 496.2 Å		0.83 Å/pix.
x 600 pix.
= 496.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.827 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.04
Minimum - Maximum-0.101225875 - 0.2843978
Average (Standard dev.)0.00011772973 (±0.0040854965)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions600600600
Spacing600600600
CellA=B=C: 496.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_55594_msk_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_55594_half_map_1.map
Projections & Slices
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Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_55594_half_map_2.map
Projections & Slices
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Sample components

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Entire : Chlorophyll f-containing monomeric far-red Photosystem II

EntireName: Chlorophyll f-containing monomeric far-red Photosystem II
Components
  • Complex: Chlorophyll f-containing monomeric far-red Photosystem II
    • Protein or peptide: PsbA
    • Protein or peptide: PsbB
    • Protein or peptide: PsbC
    • Protein or peptide: PsbD
    • Protein or peptide: PsbE
    • Protein or peptide: PsbF
    • Protein or peptide: PsbH
    • Protein or peptide: PsbI
    • Protein or peptide: PsbJ
    • Protein or peptide: PsbK
    • Protein or peptide: PsbL
    • Protein or peptide: PsbM
    • Protein or peptide: PsbY
    • Protein or peptide: PsbT
    • Protein or peptide: PsbX
    • Protein or peptide: Ycf12
    • Protein or peptide: PsbZ
  • Ligand: CA-MN4-O5 CLUSTER
  • Ligand: FE (II) ION
  • Ligand: CHLORIDE ION
  • Ligand: CHLOROPHYLL A
  • Ligand: CHLOROPHYLL D
  • Ligand: PHEOPHYTIN A
  • Ligand: BETA-CAROTENE
  • Ligand: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
  • Ligand: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE
  • Ligand: DODECYL-BETA-D-MALTOSIDE
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: Chlorophyll F
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
  • Ligand: BICARBONATE ION
  • Ligand: PROTOPORPHYRIN IX CONTAINING FE
  • Ligand: water

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Supramolecule #1: Chlorophyll f-containing monomeric far-red Photosystem II

SupramoleculeName: Chlorophyll f-containing monomeric far-red Photosystem II
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#17
Details: Chlorophyll f-containing monomeric far-red Photosystem II from Calothrix sp. NIES-3974
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)

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Macromolecule #1: PsbA

MacromoleculeName: PsbA / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 40.209117 KDa
SequenceString: MTTISQPKTT RFPAWERFCD WITSTENRLY IGWFGVLMIP LLGVSICVFV IAFIAAPPVD IDGIREPVAG SIFYGNNIIT AAVVPMSNA IGLHFYPIWE AASMDEWLYN GGPYQMIAFH YIPALACYMG REWELSYRLG MRPWIAVAYS APLAATTSVF L IYPIGQGS ...String:
MTTISQPKTT RFPAWERFCD WITSTENRLY IGWFGVLMIP LLGVSICVFV IAFIAAPPVD IDGIREPVAG SIFYGNNIIT AAVVPMSNA IGLHFYPIWE AASMDEWLYN GGPYQMIAFH YIPALACYMG REWELSYRLG MRPWIAVAYS APLAATTSVF L IYPIGQGS FSDGLPMGIS GTFNFMFVFQ AEHNILMHPL HMIGVAGVLG GSLFCAMHGS LVTSSIIRET TELESQNYGY KF GQEEETY NIVAAHGYFG RLIFQYASFN NSRSLHFFLA AWPVICIWGT AIGISTMAFN LNGFNFNNSI LDSQGRVLPS WAD VLNRAN LGFEVMHERN AHNFPLDLAS GEAIPVPVAM AAPSITM

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Macromolecule #2: PsbB

MacromoleculeName: PsbB / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 56.80173 KDa
SequenceString: MGLPWYRVHT VVLNDPGRLI AVHLMHTALT AGWAGSMALY ELALFDPSDS VFNPMWRQGM FVLPFMTRLG ITQSWAGWNI TGEAVNGFT LWTFESVAAA HIILAGLLFL AACWHWVYWD LELFQDPKSG ESFLDLPKIF GIHLLLAGLL CFGFGAFHLT G IFGPGMWV ...String:
MGLPWYRVHT VVLNDPGRLI AVHLMHTALT AGWAGSMALY ELALFDPSDS VFNPMWRQGM FVLPFMTRLG ITQSWAGWNI TGEAVNGFT LWTFESVAAA HIILAGLLFL AACWHWVYWD LELFQDPKSG ESFLDLPKIF GIHLLLAGLL CFGFGAFHLT G IFGPGMWV SDPYGLTGHI QGVAPEWGPD GFNPYNPGGV VAHHIAAGIV GIIGGIFHIS VRPPEVLYRT LRMGNIETVL AS ALATFFF AAFVVSGTMW YGTATTPIEL FGPTRYQWDS SYFQTEISRR VQTSLNQGAT LAEAWSNIPD KLAFYDYVGN SPA KGGLFR VGPMVKGDGV ALSWLGHPVF QDREGRELIV RRMPNFFETF PVVLTDKDNI VRADIPFRRA DSKLSFEQTG VTVS FLGGA LNGQTFSDPV EVKKYARRAQ LGEIFAFDTQ TFKSDGIFRT STRGWFAFAH ACWGLLWFFG HIWHGARTIF RDVFA GIDP ELDEEQVEFG VFQKVGDVST RKKQLV

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Macromolecule #3: PsbC

MacromoleculeName: PsbC / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 50.618938 KDa
SequenceString: METPLDISIT TTISPPASEG RDEPSTGYAW WAGNARFVNK SGRFLGAHVA HAGLVAFWAG AMLLFEVAHY TPEKPMYEQG LILMPHIAT LGIGVGHGGE VTDIFPFFAI AVAHLIGSAV LGFGGLYHSL KGPEKLAGFF DFDWRDKNKV TSILGFHLIA L GVAAYLLV ...String:
METPLDISIT TTISPPASEG RDEPSTGYAW WAGNARFVNK SGRFLGAHVA HAGLVAFWAG AMLLFEVAHY TPEKPMYEQG LILMPHIAT LGIGVGHGGE VTDIFPFFAI AVAHLIGSAV LGFGGLYHSL KGPEKLAGFF DFDWRDKNKV TSILGFHLIA L GVAAYLLV GKAMIWGGLY DTWAPGGGDV RLVTNPTLDP RIIFGYLLKP FTGGQGSIVS VNNLEDLVGG HIWIGGILIL GG IWHIITE PFKWTHKVFI WSGEAYLSQS LGNVAGQAII ATMFIWFNNT AYPSEFYGPT APEASQAQAL IFVVRDQKLG ANV ASAQGP TGLGKYLMRS PTGEIIFGGE TMRFWDVRAP WLEPLRGPNG LDIKKIQNDI QPWQIRRAAE YMTHAPLGSL NSVG GVATE INSFNFVSPR TWLAAAHFVF FLLFLVGHLW HAGRARAAAA GFERGLDRQD EPVLSMKPLD

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Macromolecule #4: PsbD

MacromoleculeName: PsbD / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 39.942695 KDa
SequenceString: MTIAIRPTQS NWRWFYILDD WLKRDRFVFI GWSGLLLFPC AYLAIGGWFT GTTFVSSWYT HGVVSSYLEG CNFLTAAVST PADSMGHSL LLLWGPEAEG NITRWFQIGG LWNFVAFHGV FGLIGFMLRQ FEIARTIGIR PYNAIAFSAP IAVFVSVFLI Y PLGQSSWF ...String:
MTIAIRPTQS NWRWFYILDD WLKRDRFVFI GWSGLLLFPC AYLAIGGWFT GTTFVSSWYT HGVVSSYLEG CNFLTAAVST PADSMGHSL LLLWGPEAEG NITRWFQIGG LWNFVAFHGV FGLIGFMLRQ FEIARTIGIR PYNAIAFSAP IAVFVSVFLI Y PLGQSSWF FAPSFGVAAI FRFLLFFQAF HNYTLNPFHM MGVAGVLGGA LLCAIHGATV ENTLFKDTTG FNTFRGFSTT QA EETYSFV TANRYWSQIF GIAFSNKRWL HFFMLFVPVT GLWMSAIGMV GLAFNLRAYD FISQELRAAE DPEFETFYTK NIL LNEGAR AWMAPQDQPH EHFQFPEEVL PRGNAL

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Macromolecule #5: PsbE

MacromoleculeName: PsbE / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 9.382646 KDa
SequenceString:
MSGTTGERPF GDIITSIRYW VIHSITIPAL FIAGWLFVST GLAYDVFGTP RPNEYFTQTR QELPIVSDRY ESKKQVEQFI GK

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Macromolecule #6: PsbF

MacromoleculeName: PsbF / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 5.224085 KDa
SequenceString:
MTSSNNLNQP VQQYPIFTVR WLAVHTLAVP TVFFLGAIAA MQFIQR

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Macromolecule #7: PsbH

MacromoleculeName: PsbH / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 7.636077 KDa
SequenceString:
MAQRTRLGDI LRPLNSEYGK VAPGWGTTPL MAVFMGLFFV FLLIILQLYN KSIIIQDVMV DWRSLGN

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Macromolecule #8: PsbI

MacromoleculeName: PsbI / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 4.36712 KDa
SequenceString:
(FME)LALKVVVYI VVAFFVSLFV FGFLSNDPAR NPGRRDFE

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Macromolecule #9: PsbJ

MacromoleculeName: PsbJ / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 3.872558 KDa
SequenceString:
MSGGGRIPLW IVATVAGLGV ITVVGIFFYG AYAGLGSSI

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Macromolecule #10: PsbK

MacromoleculeName: PsbK / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 5.0621 KDa
SequenceString:
MEAAILLAKL PEAYQIFDPL VDVLPIIPVF FLLLAFVWQA AVGFR

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Macromolecule #11: PsbL

MacromoleculeName: PsbL / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 4.54625 KDa
SequenceString:
(FME)ERTPNPNNQ PVELNRTSLF LGLLLIFVLG ILFSSYFFN

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Macromolecule #12: PsbM

MacromoleculeName: PsbM / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 4.299983 KDa
SequenceString:
(FME)QVNDLGFVA SLLFVLVPSV FLIILYTLTA SREGGQKDN

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Macromolecule #13: PsbY

MacromoleculeName: PsbY / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 4.444227 KDa
SequenceString:
MDIDFRIAIV LAPIAVAAGW AVFNIGRAAL VQLQNFLNKE A

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Macromolecule #14: PsbT

MacromoleculeName: PsbT / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 3.973784 KDa
SequenceString:
(FME)ESIAYVLIF AVTLGILFFA IAFREPPRIQ KKEK

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Macromolecule #15: PsbX

MacromoleculeName: PsbX / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 4.381142 KDa
SequenceString:
(FME)TPSLANFLW SLFWGTVIVV IPATVALIFI SQSDKIQRS

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Macromolecule #16: Ycf12

MacromoleculeName: Ycf12 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 4.421418 KDa
SequenceString:
MFEALASIKW EVIFQLTAVA LIMLSGPIVI FLLAVRKGDL

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Macromolecule #17: PsbZ

MacromoleculeName: PsbZ / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Calothrix sp. NIES-3974 (bacteria)
Molecular weightTheoretical: 6.740188 KDa
SequenceString:
MTFIFQFALL ALVVVSFAMV IGVPVAYATP QSWVESKKLL WLGSAVWAAL VVLVGILNFL VV

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Macromolecule #18: CA-MN4-O5 CLUSTER

MacromoleculeName: CA-MN4-O5 CLUSTER / type: ligand / ID: 18 / Number of copies: 1 / Formula: OEX
Molecular weightTheoretical: 339.827 Da
Chemical component information

ChemComp-OEX:
CA-MN4-O5 CLUSTER

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Macromolecule #19: FE (II) ION

MacromoleculeName: FE (II) ION / type: ligand / ID: 19 / Number of copies: 1 / Formula: FE2
Molecular weightTheoretical: 55.845 Da

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Macromolecule #20: CHLORIDE ION

MacromoleculeName: CHLORIDE ION / type: ligand / ID: 20 / Number of copies: 1 / Formula: CL
Molecular weightTheoretical: 35.453 Da

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Macromolecule #21: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 21 / Number of copies: 32 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A

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Macromolecule #22: CHLOROPHYLL D

MacromoleculeName: CHLOROPHYLL D / type: ligand / ID: 22 / Number of copies: 1 / Formula: CL7
Molecular weightTheoretical: 895.462 Da
Chemical component information

ChemComp-CL7:
CHLOROPHYLL D

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Macromolecule #23: PHEOPHYTIN A

MacromoleculeName: PHEOPHYTIN A / type: ligand / ID: 23 / Number of copies: 2 / Formula: PHO
Molecular weightTheoretical: 871.2 Da
Chemical component information

ChemComp-PHO:
PHEOPHYTIN A

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Macromolecule #24: BETA-CAROTENE

MacromoleculeName: BETA-CAROTENE / type: ligand / ID: 24 / Number of copies: 10 / Formula: BCR
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-BCR:
BETA-CAROTENE

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Macromolecule #25: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

MacromoleculeName: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
type: ligand / ID: 25 / Number of copies: 3 / Formula: SQD
Molecular weightTheoretical: 795.116 Da
Chemical component information

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

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Macromolecule #26: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...

MacromoleculeName: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE
type: ligand / ID: 26 / Number of copies: 2 / Formula: PL9
Molecular weightTheoretical: 749.201 Da
Chemical component information

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE

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Macromolecule #27: DODECYL-BETA-D-MALTOSIDE

MacromoleculeName: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 27 / Number of copies: 4 / Formula: LMT
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

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Macromolecule #28: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 28 / Number of copies: 7 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Macromolecule #29: Chlorophyll F

MacromoleculeName: Chlorophyll F / type: ligand / ID: 29 / Number of copies: 2 / Formula: F6C
Molecular weightTheoretical: 905.457 Da
Chemical component information


ChemComp, No image

ChemComp-F6C:
Chlorophyll F

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Macromolecule #30: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 30 / Number of copies: 7 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

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Macromolecule #31: DIGALACTOSYL DIACYL GLYCEROL (DGDG)

MacromoleculeName: DIGALACTOSYL DIACYL GLYCEROL (DGDG) / type: ligand / ID: 31 / Number of copies: 5 / Formula: DGD
Molecular weightTheoretical: 949.299 Da
Chemical component information

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

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Macromolecule #32: BICARBONATE ION

MacromoleculeName: BICARBONATE ION / type: ligand / ID: 32 / Number of copies: 1 / Formula: BCT
Molecular weightTheoretical: 61.017 Da
Chemical component information

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM

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Macromolecule #33: PROTOPORPHYRIN IX CONTAINING FE

MacromoleculeName: PROTOPORPHYRIN IX CONTAINING FE / type: ligand / ID: 33 / Number of copies: 1 / Formula: HEM
Molecular weightTheoretical: 616.487 Da
Chemical component information

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

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Macromolecule #34: water

MacromoleculeName: water / type: ligand / ID: 34 / Number of copies: 117 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 6.5
Details: 50 mM MES, 1 M betaine monohydrate, 20 mM CaCl2, 5 mM MgCl2 and 10% (v/v) glycerol, pH 6.8 adjusted with NaOH
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.4 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.33 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 285844
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION
FSC plot (resolution estimation)

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