ジャーナル: Science / 年: 2012 タイトル: Coagulation factor X activates innate immunity to human species C adenovirus. 著者: Konstantin Doronin / Justin W Flatt / Nelson C Di Paolo / Reeti Khare / Oleksandr Kalyuzhniy / Mauro Acchione / John P Sumida / Umeharu Ohto / Toshiyuki Shimizu / Sachiko Akashi-Takamura / ...著者: Konstantin Doronin / Justin W Flatt / Nelson C Di Paolo / Reeti Khare / Oleksandr Kalyuzhniy / Mauro Acchione / John P Sumida / Umeharu Ohto / Toshiyuki Shimizu / Sachiko Akashi-Takamura / Kensuke Miyake / James W MacDonald / Theo K Bammler / Richard P Beyer / Frederico M Farin / Phoebe L Stewart / Dmitry M Shayakhmetov / 要旨: Although coagulation factors play a role in host defense for "living fossils" such as horseshoe crabs, the role of the coagulation system in immunity in higher organisms remains unclear. We modeled ...Although coagulation factors play a role in host defense for "living fossils" such as horseshoe crabs, the role of the coagulation system in immunity in higher organisms remains unclear. We modeled the interface of human species C adenovirus (HAdv) interaction with coagulation factor X (FX) and introduced a mutation that abrogated formation of the HAdv-FX complex. In vivo genome-wide transcriptional profiling revealed that FX-binding-ablated virus failed to activate a distinct network of nuclear factor κB-dependent early-response genes that are activated by HAdv-FX complex downstream of TLR4/MyD88/TRIF/TRAF6 signaling. Our study implicates host factor "decoration" of the virus as a mechanism to trigger an innate immune sensor that responds to a misplacement of coagulation FX from the blood into intracellular macrophage compartments upon virus entry into the cell.
ダウンロード / ファイル: emd_5494.map.gz / 形式: CCP4 / 大きさ: 976.6 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Reconstruction of Ad5 with short fiber in complex with factor X
ボクセルのサイズ
X=Y=Z: 2.25 Å
密度
表面レベル
登録者による: 0.995 / ムービー #1: 0.995
最小 - 最大
0.82250977 - 1.2732873
平均 (標準偏差)
0.98747128 (±0.0360664)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-320
-320
-320
サイズ
640
640
640
Spacing
640
640
640
セル
A=B=C: 1440.0 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
2.25
2.25
2.25
M x/y/z
640
640
640
origin x/y/z
0.000
0.000
0.000
length x/y/z
1440.000
1440.000
1440.000
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-50
29
166
NX/NY/NZ
106
122
134
MAP C/R/S
1
2
3
start NC/NR/NS
-320
-320
-320
NC/NR/NS
640
640
640
D min/max/mean
0.823
1.273
0.987
-
添付データ
-
試料の構成要素
-
全体 : Complex of Ad5-short-fiber with factor X
全体
名称: Complex of Ad5-short-fiber with factor X
要素
試料: Complex of Ad5-short-fiber with factor X
ウイルス: Human adenovirus 5 (ヒトアデノウイルス)
-
超分子 #1000: Complex of Ad5-short-fiber with factor X
超分子
名称: Complex of Ad5-short-fiber with factor X / タイプ: sample / ID: 1000 / 集合状態: 240 factor X molecules bind to one Ad virion / Number unique components: 2
分子量
理論値: 164 MDa
-
超分子 #1: Human adenovirus 5
超分子
名称: Human adenovirus 5 / タイプ: virus / ID: 1 / Name.synonym: Human adenovirus type 5 with short fiber / NCBI-ID: 28285 / 生物種: Human adenovirus 5 / データベース: NCBI / ウイルスタイプ: VIRION / ウイルス・単離状態: OTHER / ウイルス・エンベロープ: No / ウイルス・中空状態: No / Syn species name: Human adenovirus type 5 with short fiber