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- EMDB-54174: D. melanogaster Augmin TII N-clamp bound to a microtubule -

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Basic information

Entry
Database: EMDB / ID: EMD-54174
TitleD. melanogaster Augmin TII N-clamp bound to a microtubule
Map dataCryo-EM reconstruction of the microtubule-bound D. melanogaster Augmin TII N-clamp filtered to local resolution.
Sample
  • Complex: Microtubule decorated with D. melanogaster N-clamp
    • Complex: Heterotetrameric Augmin TII N-clamp complex
    • Organelle or cellular component: Microtubule
Keywordsaugmin / N-clamp / TII / microtubule / branching / nucleation / HAUS / HAUS augmin-like complex subunit / HAUS6 / HAUS7 / HAUS2 / HAUS8 / Dgt6 / Msd5 / Dgt4 / Msd1 / Drosophila / melanogaster / complex / CH domain / calponin homology domain / CELL CYCLE
Biological speciesDrosophila melanogaster (fruit fly) / Sus scrofa (pig)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.7 Å
AuthorsWuertz M / Vermeulen BJA / Tonon G / Pfeffer S
Funding support Germany, 12 items
OrganizationGrant numberCountry
German Research Foundation (DFG)Sch Baden-Wuerttemberg Germany
German Research Foundation (DFG)INST 35/1314-1 FUGG Germany
German Research Foundation (DFG)INST 35/1503-1 FUGG Germany
German Research Foundation (DFG)INST 35/1134-1 FUGG Germany
German Research Foundation (DFG)DFG Schi 295/4-4 Germany
German Research Foundation (DFG)SCHI 295/11-1 Germany
German Research Foundation (DFG)SCHI 295/9-1 Germany
German Research Foundation (DFG)DFG PF 963/1-4 Germany
German Research Foundation (DFG)DFG PF 963/4-1 Germany
Other private
Other private
Other government
CitationJournal: To Be Published / Year: 2025
Title: Structural insights into the augmin-microtubule interaction reveal a conserved function of the HAUS6 CH domain in orienting augmin on microtubules
Authors: Wuertz M / Tonon G / Vermeulen BJA / Zezlina M / Gao G / Annett N / Seidl A / Koenig M / Harkenthal M / Eustermann S / Erhardt S / Lolicato F / Schiebel E / Pfeffer S
History
DepositionJun 25, 2025-
Header (metadata) releaseAug 27, 2025-
Map releaseAug 27, 2025-
UpdateAug 27, 2025-
Current statusAug 27, 2025Processing site: PDBe / Status: Released

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Structure visualization

Downloads & links

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Map

FileDownload / File: emd_54174.map.gz / Format: CCP4 / Size: 307.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCryo-EM reconstruction of the microtubule-bound D. melanogaster Augmin TII N-clamp filtered to local resolution.
Voxel sizeX=Y=Z: 1.069 Å
Density
Contour LevelBy AUTHOR: 0.117
Minimum - Maximum-0.32897013 - 0.6063111
Average (Standard dev.)0.008043983 (±0.045612045)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions432432432
Spacing432432432
CellA=B=C: 461.808 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Microtubule decorated with D. melanogaster N-clamp

EntireName: Microtubule decorated with D. melanogaster N-clamp
Components
  • Complex: Microtubule decorated with D. melanogaster N-clamp
    • Complex: Heterotetrameric Augmin TII N-clamp complex
    • Organelle or cellular component: Microtubule

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Supramolecule #1: Microtubule decorated with D. melanogaster N-clamp

SupramoleculeName: Microtubule decorated with D. melanogaster N-clamp / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3
Source (natural)Organism: Drosophila melanogaster (fruit fly)

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Supramolecule #2: Heterotetrameric Augmin TII N-clamp complex

SupramoleculeName: Heterotetrameric Augmin TII N-clamp complex / type: complex / ID: 2 / Parent: 1
Source (natural)Organism: Drosophila melanogaster (fruit fly)

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Supramolecule #3: Microtubule

SupramoleculeName: Microtubule / type: organelle_or_cellular_component / ID: 3 / Parent: 1
Source (natural)Organism: Sus scrofa (pig) / Organ: Brain

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statehelical array

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Sample preparation

BufferpH: 6.8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 49.8 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
In silico model: Synthetic reference provided under https://github.com/moores-lab/MiRPv2/tree/main/data/protofilament_sorting_references
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.5.3) / Number images used: 469266
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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