|Entry||Database: EMDB / ID: 5414|
|Title||CryoEM map of the immature human astrovirus HAstV-8|
|Sample||immature human astrovirus HAstV-8|
|Source||Human astrovirus / virus|
|Map data||reconstruction of immature human astrovirus HAstV-8|
|Method||single particle (icosahedral) reconstruction, at 25 Å resolution|
|Authors||Dryden KA / Tihova M / Nowotny N / Matsui SM / Mendez E / Yeager M|
|Citation||J. Mol. Biol., 2012, 422, 650-658|
|Date||Deposition: Apr 24, 2012 / Header (metadata) release: Jun 28, 2012 / Map release: Jun 28, 2012 / Last update: Nov 7, 2012|
Downloads & links
|File||emd_5414.map.gz (map file in CCP4 format, 154892 KB)|
|Projections & slices|
Images are generated by Spider package.
|Voxel size||X=Y=Z: 1.56 Å|
CCP4 map header:
-Entire immature human astrovirus HAstV-8
|Entire||Name: immature human astrovirus HAstV-8 / Number of components: 1|
-Component #1: virus, Human astrovirus
|Sample solution||Buffer solution: 50mM Tris-HCl, pH 7.4, 100mM NaCl, 10mM EDTA|
|Support film||glow discharged with amylamine|
|Vitrification||Instrument: HOMEMADE PLUNGER / Cryogen name: ETHANE / Method: watch drop spread on filter paper before plunging|
-Electron microscopy imaging
|Imaging||Microscope: FEI/PHILIPS CM120T / Date: Dec 22, 2003|
|Electron gun||Electron source: LAB6 / Accelerating voltage: 120 kV / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 45000 X (nominal) / Imaging mode: BRIGHT FIELD / Defocus: 1450 - 1850 nm|
|Specimen Holder||Model: GATAN LIQUID NITROGEN|
|Camera||Detector: KODAK SO-163 FILM|
|Image acquisition||Number of digital images: 7 / Scanner: ZEISS SCAI / Sampling size: 7 microns|
|Processing||Method: single particle (icosahedral) reconstruction|
Details: Particles were boxed with X3D and processed with Auto3dem
Number of projections: 451 / Applied symmetry: I (icosahedral)
|3D reconstruction||Algorithm: polar Fourier transform / Software: Auto3dem / Resolution: 25 Å / Resolution method: FSC 0.5|
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