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- EMDB-53347: Structure of the 50S ribosomal subunit from the antibiotic-produc... -

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Open data

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Basic information

Entry

Database: EMDB / ID: EMD-53347

Title

Structure of the 50S ribosomal subunit from the antibiotic-producing bacterium Streptomyces fradiae

Map data

Sample

Complex: Streptomyces fradiae ribosome
- Protein or peptide: x 28 types
- RNA: x 2 types

Keywords

Streptomyces / ribosome / 50S / ribosome-targeting antibiotics

Function / homology

Function and homology information

large ribosomal subunit / transferase activity / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding ...
Similarity search - Function

Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / : / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. ...Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / : / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L9 signature. / : / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / : / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / : / Ribosomal protein L6, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L30, bacterial-type / : / L28p-like / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L20 / Ribosomal L32p protein family / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L19 superfamily / Ribosomal protein L21 / Ribosomal protein L32p / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal protein L34 / Ribosomal protein L34 / Ribosomal protein L24 / Ribosomal protein L13, bacterial-type / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / 50S ribosomal protein uL4 / Ribosomal protein L23/L25, conserved site / Ribosomal protein L23 signature. / Ribosomal protein L30, conserved site / Ribosomal protein L30 signature. / Ribosomal protein L5, conserved site
Similarity search - Domain/homology

Biological species

Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)

Method

single particle reconstruction / cryo EM / Resolution: 3.13 Å

Authors

Ekemezie CL / Melnikov SV

Funding support

United Kingdom, 2 items

Citation

Journal: To Be Published
Title: Evolution of drug-binding sites in bacterial ribosomes
Authors: Ekemezie CL / Chan LI / Brown CR / Helena-Bueno K / Williams TA / Melnikov SV

History

Deposition	Apr 7, 2025	-
Header (metadata) release	May 14, 2025	-
Map release	May 14, 2025	-
Update	May 14, 2025	-
Current status	May 14, 2025	Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images	emd_53347.png

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Downloads & links

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EMDB archive

Map data	emd_53347.map.gz	257.3 MB		EMDB map data format
Header (meta data)	emd-53347-v30.xml emd-53347.xml	51.1 KB 51.1 KB	Display Display	EMDB header
FSC (resolution estimation)	emd_53347_fsc.xml	17 KB	Display	FSC data file
Images	emd_53347.png	85.7 KB
Filedesc metadata	emd-53347.cif.gz	10.9 KB
Others	emd_53347_half_map_1.map.gz emd_53347_half_map_2.map.gz	475.7 MB 475.7 MB
Archive directory	http://ftp.pdbj.org/pub/emdb/structures/EMD-53347 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-53347	HTTPS FTP

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Validation report

Summary document	emd_53347_validation.pdf.gz	1.3 MB	Display	EMDB validaton report
Full document	emd_53347_full_validation.pdf.gz	1.3 MB	Display
Data in XML	emd_53347_validation.xml.gz	26.8 KB	Display
Data in CIF	emd_53347_validation.cif.gz	35.2 KB	Display
Arichive directory	https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-53347 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-53347	HTTPS FTP

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Related structure data

Related structure data	9qt5MC M: atomic model generated by this map C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

EMDB pages	EMDB (EBI/PDBe) / EMDataResource
Related items in Molecule of the Month	#253 - Jan 2021 Expressome similarity (15) #121 - Jan 2010 70S Ribosomes similarity (11) #157 - Jan 2013 Transfer-Messenger RNA similarity (18) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (13)

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Map

File

Download / File: emd_53347.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)

Projections & slices

Image control

Size
Brightness
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Others	InvertY flip

Axes	Z (Sec.)	Y (Row.)	X (Col.)
	0.93 Å/pix. x 512 pix. = 478.208 Å	0.93 Å/pix. x 512 pix. = 478.208 Å	0.93 Å/pix. x 512 pix. = 478.208 Å
Surface
Projections
Slices (1/3)
Slices (1/2)
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Images are generated by Spider.

Voxel size

X=Y=Z: 0.934 Å

Density

Histogram

Histogram (log scale)

Contour Level	By AUTHOR: 0.0769
Minimum - Maximum	-0.1749859 - 0.44742438
Average (Standard dev.)	-0.000068282825 (±0.018293345)

Symmetry

Space group: 1

Details

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Supplemental data

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Half map: #2

File

emd_53347_half_map_1.map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

File

emd_53347_half_map_2.map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Streptomyces fradiae ribosome

Entire	Name: Streptomyces fradiae ribosome
Components	Complex: Streptomyces fradiae ribosome Protein or peptide: Large ribosomal subunit protein uL16 RNA: 5S rRNA Protein or peptide: Large ribosomal subunit protein uL2 Protein or peptide: Large ribosomal subunit protein uL3 Protein or peptide: Large ribosomal subunit protein uL4 Protein or peptide: Large ribosomal subunit protein uL5 Protein or peptide: Large ribosomal subunit protein uL6 Protein or peptide: Large ribosomal subunit protein bL9 Protein or peptide: Large ribosomal subunit protein uL13 Protein or peptide: Large ribosomal subunit protein uL14 Protein or peptide: Large ribosomal subunit protein uL18 Protein or peptide: Large ribosomal subunit protein uL15 Protein or peptide: Large ribosomal subunit protein bL19 Protein or peptide: Large ribosomal subunit protein bL20 Protein or peptide: Large ribosomal subunit protein bL21 Protein or peptide: Large ribosomal subunit protein uL22 Protein or peptide: Large ribosomal subunit protein uL23 Protein or peptide: Large ribosomal subunit protein uL24 Protein or peptide: Large ribosomal subunit protein bL25 Protein or peptide: Large ribosomal subunit protein bL27 Protein or peptide: Large ribosomal subunit protein bL28 Protein or peptide: Large ribosomal subunit protein uL29 Protein or peptide: Large ribosomal subunit protein uL30 Protein or peptide: Large ribosomal subunit protein bL32 Protein or peptide: Large ribosomal subunit protein bL33 Protein or peptide: Large ribosomal subunit protein bL34 Protein or peptide: Large ribosomal subunit protein bL35 Protein or peptide: Large ribosomal subunit protein bL36 Protein or peptide: Large ribosomal subunit protein bL17 RNA: 23S rRNA

Entire

Name: Streptomyces fradiae ribosome

Components

Complex: Streptomyces fradiae ribosome
- Protein or peptide: Large ribosomal subunit protein uL16
- RNA: 5S rRNA
- Protein or peptide: Large ribosomal subunit protein uL2
- Protein or peptide: Large ribosomal subunit protein uL3
- Protein or peptide: Large ribosomal subunit protein uL4
- Protein or peptide: Large ribosomal subunit protein uL5
- Protein or peptide: Large ribosomal subunit protein uL6
- Protein or peptide: Large ribosomal subunit protein bL9
- Protein or peptide: Large ribosomal subunit protein uL13
- Protein or peptide: Large ribosomal subunit protein uL14
- Protein or peptide: Large ribosomal subunit protein uL18
- Protein or peptide: Large ribosomal subunit protein uL15
- Protein or peptide: Large ribosomal subunit protein bL19
- Protein or peptide: Large ribosomal subunit protein bL20
- Protein or peptide: Large ribosomal subunit protein bL21
- Protein or peptide: Large ribosomal subunit protein uL22
- Protein or peptide: Large ribosomal subunit protein uL23
- Protein or peptide: Large ribosomal subunit protein uL24
- Protein or peptide: Large ribosomal subunit protein bL25
- Protein or peptide: Large ribosomal subunit protein bL27
- Protein or peptide: Large ribosomal subunit protein bL28
- Protein or peptide: Large ribosomal subunit protein uL29
- Protein or peptide: Large ribosomal subunit protein uL30
- Protein or peptide: Large ribosomal subunit protein bL32
- Protein or peptide: Large ribosomal subunit protein bL33
- Protein or peptide: Large ribosomal subunit protein bL34
- Protein or peptide: Large ribosomal subunit protein bL35
- Protein or peptide: Large ribosomal subunit protein bL36
- Protein or peptide: Large ribosomal subunit protein bL17
- RNA: 23S rRNA

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Supramolecule #1: Streptomyces fradiae ribosome

Supramolecule	Name: Streptomyces fradiae ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#30
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 2.5 MDa

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Macromolecule #1: Large ribosomal subunit protein uL16

Macromolecule	Name: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 15.829605 KDa
Sequence	String: MLIPRRVKHR KQHHPKRSGM SKGGTQVAFG EYGIQALTPA YVTNRQIEAA RIAMTRHIKR GGKVWINIYP DRPLTKKPAE TRMGSGKGS PEWWVANVKP GRVMFELSYP NEKIAREALT RAAHKLPMKC RIVRREAGEA UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #3: Large ribosomal subunit protein uL2

Macromolecule	Name: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 30.492934 KDa
Sequence	String: MGIRKYKPTT PGRRGSSVAD FVEITRSTPE KSLVRPLHSK GGRNNTGRVT VRHQGGGHKR AYRVIDFRRH DKDGVPAKVA HIEYDPNRT ARIALLHYAD GEKRYIIAPR GLSQGDRIEN GPGADIKPGN NLALRNIPVG TTIHAIELRP GGGAKFARSA G ASVQLLAK ...String: MGIRKYKPTT PGRRGSSVAD FVEITRSTPE KSLVRPLHSK GGRNNTGRVT VRHQGGGHKR AYRVIDFRRH DKDGVPAKVA HIEYDPNRT ARIALLHYAD GEKRYIIAPR GLSQGDRIEN GPGADIKPGN NLALRNIPVG TTIHAIELRP GGGAKFARSA G ASVQLLAK EGSMAHLRMP SGEIRLVDVR CRATIGEVGN AEQSNINWGK AGRKRWLGVR PTVRGVAMNP VDHPHGGGEG KT SGGRHPV SPWGQKEGRT RSPKKASNKY IVRRRKTNKK R UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #4: Large ribosomal subunit protein uL3

Macromolecule	Name: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 22.682186 KDa
Sequence	String: MAKNIKGVLG EKLGMTQVWD ENNRVVPVTV VKAGPCVVTQ VRSNDTDGYE SVQIAFGEID PRKVNKPLKG HFAKADVTPR RHLVEIRTA DASEYTLGQE ITAAVFEAGV KVDVTGKSKG KGFAGVMKRH NFRGLGAGHG TQRKHRSPGS IGGCATPGRV F KGLRMAGR ...String: MAKNIKGVLG EKLGMTQVWD ENNRVVPVTV VKAGPCVVTQ VRSNDTDGYE SVQIAFGEID PRKVNKPLKG HFAKADVTPR RHLVEIRTA DASEYTLGQE ITAAVFEAGV KVDVTGKSKG KGFAGVMKRH NFRGLGAGHG TQRKHRSPGS IGGCATPGRV F KGLRMAGR MGNERVTTQN LTVHAVDAEK GLLLIKGAVP GPNGGLVLVR TAAKGA UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #5: Large ribosomal subunit protein uL4

Macromolecule	Name: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 23.112234 KDa
Sequence	String: MSTIDILSPA GDKAGTVELP SEIFDAKTSV PLIHQVVVAQ LAAARQGTHK TKTRGEVRGG GKKPYRQKGT GRARQGSTRA PQFAGGGVV HGPVPRDYSQ RTPKKMKAAA LRGALSDRAR HSRIHVVTGV VEGEISTKAA KTLFGKISER KNLLLVVERS D EAAWLSAR ...String: MSTIDILSPA GDKAGTVELP SEIFDAKTSV PLIHQVVVAQ LAAARQGTHK TKTRGEVRGG GKKPYRQKGT GRARQGSTRA PQFAGGGVV HGPVPRDYSQ RTPKKMKAAA LRGALSDRAR HSRIHVVTGV VEGEISTKAA KTLFGKISER KNLLLVVERS D EAAWLSAR NLPQVHILEP GQLNTYDVLV SDDVVFTKAA FESFVSGPQT AETEGSDA UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #6: Large ribosomal subunit protein uL5

Macromolecule	Name: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 20.97108 KDa
Sequence	String: MATTTTPRLK TKYREEIVGK LRDEFSYENV MQVPGLVKIV VNMGVGDAAR DSKLIEGAIR DLTTITGQKP AVTKARKSIA QFKLREGQP IGAHVTLRGD RMWEFLDRTL SLALPRIRDF RGLSPKQFDG RGNYTFGLTE QVMFHEIDQD KIDRVRGMDI T VVTTATND DEGRALLRHL GFPFKEN UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #7: Large ribosomal subunit protein uL6

Macromolecule	Name: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 19.174201 KDa
Sequence	String: MSRIGKLPIT VPAGVDVTID GRTVEVKGPK GTLTHTVAAP IEIVKGEDGV LNVTRPNDER QNKALHGLSR TLVANMITGV TQGYTKALE ISGVGYRVAA KGSNLEFQLG YSHPILVEAP EGISFKVESP TKFSVEGIDK QKVGEVAANI RKLRKPDPYK A KGVKYAGE VIRRKVGKAG K UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #8: Large ribosomal subunit protein bL9

Macromolecule	Name: Large ribosomal subunit protein bL9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 15.794217 KDa
Sequence	String: MKIILTHEVS GLGAAGDVVD VKDGYARNYL VPRGFAIRWT KGGEKDVAQI RRARKIHEIA TIEQANQIKA QLESVKVRLA VRSGDAGRL FGSVTPADIA SAIKSAGGPE VDKRRVELGS PIKTLGSHQV SVRLHPEVAA KLGVEVVAA UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #9: Large ribosomal subunit protein uL13

Macromolecule	Name: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 16.301764 KDa
Sequence	String: MRTYSPKPGD VTRQWHVIDA QDVVLGRLAT TAATLLRGKH KPIYAPHVDA GDFVIIINAD KVHLSGNKRT QKMAYRHSGY PGGLRSVRY DDLLANNPEK AVEKAVKGML PKNSLGRQML SKLKVYAGEN HPHAAQQPVP FEITQVAQ UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #10: Large ribosomal subunit protein uL14

Macromolecule	Name: Large ribosomal subunit protein uL14 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 13.426666 KDa
Sequence	String: MIQQESRLRV ADNTGAKEIL CIRVLGGSGR RYAGIGDVIV ATVKDAIPGG NVKKGDVVKA VIVRTVKERR RQDGSYIRFD ENAAVILKN DGDPRGTRIF GPVGRELREK KFMKIISLAP EVL UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #11: Large ribosomal subunit protein uL18

Macromolecule	Name: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 13.341402 KDa
Sequence	String: MAYGVKIAKG KAYKGAALKR RHIRIRKKVS GTAERPRLVV TRSNRGITAQ VIDDIAGHTV ASASTLDASI RGGEGDKSAK AQQVGKLVA ERAKAAGVEA VVFDRGGNRY AGRIAALADA AREAGLKF UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #12: Large ribosomal subunit protein uL15

Macromolecule	Name: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 16.01954 KDa
Sequence	String: MAENNPLKIH NLRPAPGAKT AKTRVGRGEA SKGKTAGRGT KGTKARYQVP ERFEGGQMPL HMRLPKLKGF KNPFKTEYQV VNLDKLASL YPEGGEVTVA DLVAKGAVRK NQLVKVLGQG EVSVALQVTV DAVSGSAKEK ITAAGGSVTE LV UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #13: Large ribosomal subunit protein bL19

Macromolecule	Name: Large ribosomal subunit protein bL19 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 13.173204 KDa
Sequence	String: MSHVLDAVNA ASLRSDVPAF RPGDTVNVHV RVIEGNRSRI QQFKGVVIRR QGSGVSETFT VRKVSFSVGV ERTFPVHSPI FEKIELVTR GDVRRAKLYY LRELRGKAAK IKEKREN UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #14: Large ribosomal subunit protein bL20

Macromolecule	Name: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 14.460655 KDa
Sequence	String: MARVKRAVNA HKKRRAILEQ ASGYRGQRSR LYRKAKEQVT HSLVYNYNDR KKRKGDFRQL WIQRINAAAR ANGITYNRFI QGLKAANVE VDRKILAELA VNDANAFAAL VEVAQKALPS DVNAPKAAA UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #15: Large ribosomal subunit protein bL21

Macromolecule	Name: Large ribosomal subunit protein bL21 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 11.600355 KDa
Sequence	String: MYAIVRSGGR QHKVAVGDIV EVDKISTAKV GDTVELSTLL VVDGDAVTSD PWVLAGIKVQ AEVVDHHKGA KIDILRYKNK TGYRRRQGH RQQYTAIKVT AIPTAAK UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #16: Large ribosomal subunit protein uL22

Macromolecule	Name: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 12.846766 KDa
Sequence	String: MEARAQARYI RVTPMKARRV VDLIRGMDAT EAQAVLRFAP QAASVPVKKV LDSAIANAAH NYDHTDASSL FISEAFVDEG PTLKRFRPR AQGRAYRIRK RTSHITVVVS SKEGTR UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #17: Large ribosomal subunit protein uL23

Macromolecule	Name: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 11.849599 KDa
Sequence	String: MSEAVVTSKT FTDPRDILVK PVVSEKSYAL LDENKYTFVV DPRANKTQIK QAVEAVFSVK VTGVNTINRQ GKRKRTRTGY GKRANTKRA IVTLAEGNRI DIFGGPTA UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #18: Large ribosomal subunit protein uL24

Macromolecule	Name: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 11.709555 KDa
Sequence	String: MKIKKGDLVQ VITGKDKGKQ GKVIAAFPRE DRVLVEGVNR VKKHTKANQP GRASQAGGIV TVEAPIHVSN VQLVVEKDGN KVVTRVGYR FDENGNKIRV AKRTGEDI UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #19: Large ribosomal subunit protein bL25

Macromolecule	Name: Large ribosomal subunit protein bL25 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 20.475338 KDa
Sequence	String: MSEVKITAET RSEFGKGAAR RIRRENKVPG VLYGHGTDPV HLTLPGHDLM MALKTSNVLL SLDLDGKTEL AIPKAVQRDP LKGFLEHVD LLLVKRGEQV NVEIPVVTEG ELAPGGNLLE HVLNALPVEA EATHIPESLS VSVAGLEAGA SVLAKDIALP K GVSLAVEA ...String: MSEVKITAET RSEFGKGAAR RIRRENKVPG VLYGHGTDPV HLTLPGHDLM MALKTSNVLL SLDLDGKTEL AIPKAVQRDP LKGFLEHVD LLLVKRGEQV NVEIPVVTEG ELAPGGNLLE HVLNALPVEA EATHIPESLS VSVAGLEAGA SVLAKDIALP K GVSLAVEA DTVVLQVLAA QAEEPAEGAE SAESAEA UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #20: Large ribosomal subunit protein bL27

Macromolecule	Name: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 8.844027 KDa
Sequence	String: MAHKKGASST RNGRDSNAQR LGVKRFGGQV VNAGEILVRQ RGTHFHPGAG VGRGGDDTLF ALQAGSVQFG TFRGRKVVNI VPVA UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #21: Large ribosomal subunit protein bL28

Macromolecule	Name: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 6.734838 KDa
Sequence	String: MAANCDVCGK GPGFGNNISH SHRRTSRRWN PNIQRVRAVV GRTPKRLNVC TSCIKAGKVS R UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #22: Large ribosomal subunit protein uL29

Macromolecule	Name: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 8.417594 KDa
Sequence	String: MSAGTKASEL RELSNEELVG KLREAKEELF NLRFQAATGQ LENHGRLKAV RKDIARIYTL MRERELGIET VESA UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #23: Large ribosomal subunit protein uL30

Macromolecule	Name: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 6.932001 KDa
Sequence	String: MARLKITQTK SYIGSKQNHR DTLRSLGLKG INTQVVKEDR PEFRGMVHTV RHLVTVEEVD UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #24: Large ribosomal subunit protein bL32

Macromolecule	Name: Large ribosomal subunit protein bL32 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 6.566715 KDa
Sequence	String: MAVPKRKMSR SNTRHRRSQW KAAVPTLVSC ERCQEPKQQH IACPSCGTYN KRQVLAV UniProtKB: Large ribosomal subunit protein bL32

+
Macromolecule #25: Large ribosomal subunit protein bL33

Macromolecule	Name: Large ribosomal subunit protein bL33 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 6.423511 KDa
Sequence	String: MAATDVRPKI TLACVECKER NYITKKNRRN NPDRLEMKKH CPRCNAHTAH RETR UniProtKB: Large ribosomal subunit protein bL33

+
Macromolecule #26: Large ribosomal subunit protein bL34

Macromolecule	Name: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 5.313309 KDa
Sequence	String: MSKRTFQPNN RRRAKTHGFR LRMRTRAGRA ILATRRSKGR ARLSA UniProtKB: Large ribosomal subunit protein bL34

+
Macromolecule #27: Large ribosomal subunit protein bL35

Macromolecule	Name: Large ribosomal subunit protein bL35 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 7.103505 KDa
Sequence	String: MPKNKTHSGA KKRFKITGSG KVLRERAGKR HLLEHKSSKL TRRLTGNAEM APGDSAKIKK MLGI UniProtKB: Large ribosomal subunit protein bL35

+
Macromolecule #28: Large ribosomal subunit protein bL36

Macromolecule	Name: Large ribosomal subunit protein bL36 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 4.403474 KDa
Sequence	String: MKVKPSVKKI CDKCKVIRRH GRVMVICDNL RHKQRQG UniProtKB: Large ribosomal subunit protein bL36

+
Macromolecule #29: Large ribosomal subunit protein bL17

Macromolecule	Name: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 17.701307 KDa
Sequence	String: MPKPAKGARL GGSAAHEKLL LANLAKSLFE HGRITTTEAK ARRLRPVAER LITKAKKGDI HNRRLVLQTI TDKGIVHTLF TEIAPRYAE RPGGYTRITK IGNRRGDNAP MAVIELVEGE IAKKATVAEA EAATKRAVKE ADEAAASEAP AEDTAEAPAE E KKEA UniProtKB: Large ribosomal subunit protein bL17

+
Macromolecule #2: 5S rRNA

Macromolecule	Name: 5S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 37.883551 KDa
Sequence	String: UUCGGUGGUC AUAGCGUUAG GGAAACGCCC GGUUACAUUC CGAACCCGGA AGCUAAGCCU UUCAGCGCCG AUGGUACUGC AGGGGGGAC CCUGUGGGAG AGUAGGACGC CGCCGAAC GENBANK: GENBANK: CP023696.1

+
Macromolecule #30: 23S rRNA

Macromolecule	Name: 23S rRNA / type: rna / ID: 30 / Number of copies: 1
Source (natural)	Organism: Streptomyces fradiae ATCC 10745 = DSM 40063 (bacteria)
Molecular weight	Theoretical: 1.0122666249999999 MDa
Sequence	String: GGCCAAGUUU AUAAGGGCGC ACGGUGGAUG CCUUGGCACC AGGAACCGAU GAAGGACGUG GGAGGCCGCG AUAGGCCCCG GGGAGCUGU CAACCGAGCU UUGAUCCGGG GGUGUCCGAA UGGGGAAACC CGGCAGUCGU CAUGGGCUGU CACCCACUGC U GAACACAU ...String: GGCCAAGUUU AUAAGGGCGC ACGGUGGAUG CCUUGGCACC AGGAACCGAU GAAGGACGUG GGAGGCCGCG AUAGGCCCCG GGGAGCUGU CAACCGAGCU UUGAUCCGGG GGUGUCCGAA UGGGGAAACC CGGCAGUCGU CAUGGGCUGU CACCCACUGC U GAACACAU AGGCAGUGUG GAGGGAACGA GGGGAAGUGA AACAUCUCAG UACCCUCAGG AAGAGAAAAC AACCGUGAUU CC GGGAGUA GUGGCGAGCG AAACCGGAUG AGGCCAAACC GUAUGCGUGU GAUACCCGGC AGGGGUUGCG CAUGCGGGGU UGU GGGAUC UCUCUUUCAC GGUCUGCCGG CCGUGAGACG AGUCAGAAAC CGUUGAUGUA GGCGAAGGAC AUGCGAAAGG UCCG GCGUA GAGGGUAAGA CCCCCGUAGC UGAAACAUUG ACGGCUCGUU UGAGAGACAC CCAAGUAGCA CGGGGCCCGA GAAAU CCCG UGUGAAUCUG GCGGGACCAC CCGCUAAGCC UAAAUAUUCC CUGGUGACCG AUAGCGGAUA GUACCGUGAG GGAAUG GUG AAAAGUACCG CGGGAGCGGA GUGAAAUAGU ACCUGAAACC GUGUGCCUAC AAGCCGUGGG AGCGUCGGAC AUGCUUU GC AUGUCUCGUG ACUGCGUGCC UUUUGAAGAA UGAGCCUGCG AGUUUGCGGU GCGUUGCGAG GUUAACCCGU GUGGGGAA G CCGUAGCGAA AGCGAGUCCG AAUAGGGCGA UCGAGUAGCG CGCUCAAGAC CCGAAGCGGA GUGAUCUAGC CAUGGGCAG GUUGAAGCGG AGGUAAGACU UCGUGGAGGA CCGAACCCAC CAGGGUUGAA AACCUGGGGG AUGACCUGUG GUUAGGGGUG AAAGGCCAA UCAAACUCCG UGAUAGCUGG UUCUCCCCGA AAUGCAUUUA GGUGCAGCGU CGUGUGUUUC UUGCCGGAGG U AGAGCACU GGAUAGGCGA UGGGCCCUAC CGGGUUACUG ACCUUAGCCA AACUCCGAAU GCCGGUAAGU GAGAGCGCGG CA GUGAGAC UGUGGGGGAU AAGCUCCAUG GUCGAGAGGG AAACAGCCCA GAGCAUCGAC UAAGGCCCCU AAGCGUACGC UAA GUGGGA AAGGAUGUGG AGUCGCAGAG ACAACCAGGA GGUUGGCUUA GAAGCAGCCA CCCUUGAAAG AGUGCGUAAU AGCU CACUG GUCAAGUGAU UCCGCGCCGA CAAUGUAGCG GGGCUCAAGC GUACCGCCGA AGUCGUGUCA UUGCAGCAUA AGCCC CAAC GGGUGCUGUG AUGGGUAGGG GAGCGUCGUG UGCCGGGUGA AGCAGCCGCG GAAGCGAGUU GUGGACGGUU CACGAG UGA GAAUGCAGGC AUGAGUAGCG AUACACACGU GAGAAACGUG UGCGCCGAUU GACUAAGGGU UCCUGGGUCA AGCUGAU CU GCCCAGGGUA AGUCGGGACC UAAGGCGAGG CCGACAGGCG UAGUCGAUGG ACAACCGGUU GAUAUUCCGG UACCCGCU U UGAAGCGCCA GCGCUGAACC CAGCGAUGCU AAGCCCGUGA AACCGCCGUG UGCGUCUUCG GACAAGCACG GAGUGGUGG AGCCGGUGGC CCAGACUGGU AGUAGGUGAG CGAUGGGGUG ACGCAGGAAG GUAGUCCAGC CCGGGCGGUG GUUGUCCCGG GGUAAGGGU GUAGGCCGUG UGGUAGGCAA AUCCGUCACA CGUUAAGGCU GAGACCUGAU GCCGAGCCGA UUGUGGUGAA G UGGAUGAU CCUAUGCUGU CGAGAAAAGC CUCUAGCGAG UUUCAUGGCG GCCCGUACCC UAAACCGACU CAGGUGGUCA GG UAGAGAA UACCGAGGCG UUCGGGUGAA CUAUGGUUAA GGAACUCGGC AAAAUGCCCC CGUAACUUCG GGAGAAGGGG GGC CACUUC UGGUGAUCAC UCUUGCAGUG UGAGCUGGGG GUGGCCGCAG AGACCAGCGA GAAGCGACUG UUUACUAAAA ACAC AGGUC CGUGCGAAGC CGUAAGGCGA UGUAUACGGA CUGACGCCUG CCCGGUGCUG GAACGUUAAG GGGACCGGUU AGCUU GGAU UCGUCCGGGC GAAGCUGAGA ACUUAAGCGC CAGUAAACGG CGGUGGUAAC UAUAACCAUC CUAAGGUAGC GAAAUU CCU UGUCGGGUAA GUUCCGACCU GCACGAAUGG CGUAACGACU UCUCGACUGU CUCAACCAUA GGCCCGGUGA AAUUGCA CU ACGAGUAAAG AUGCUCGUUU CGCGCAGCAG GACGGAAAGA CCCCGGGACC UUUACUACAG UUUGAUAUUG GUGUUCGG U UCGGCUUGUG UAGGAUAGGU GGGAGACUGU GAAACUGUGA CGCCAGUCAU GGUGGAGUCG UCGUUGAAAU ACCACUCUG GUCGUGCUGG AUGUCUAACC UGGGUCCGUG AUCCGGAUCA GGGACAGUGU CUGAUGGGUA GUUUAACUGG GGCGGUUGCC UCCUAAAGG GUAACGGAGG CGCCCAAAGG UUCCCUCAGC CUGGUUGGCA AUCAGGUGUU GAGUGUAAGU GCACAAGGGA G CUUGACUG UGAGACCGAC GGGUCGAGCA GGGACGAAAG UCGGGACUAG UGAUCCGGCG GUGGCUUGUG GAAGCGCCGU CG CUCAACG GAUAAAAGGU ACCCCGGGGA UAACAGGCUG AUCUUCCCCA AGAGUCCAUA UCGACGGGAU GGUUUGGCAC CUC GAUGUC GGCUCGUCGC AUCCUGGGGC UGGAGUCGGU CCCAAGGGUU GGGCUGUUCG CCCAUUAAAG CGGUACGCGA GCUG GGUUU AGAACGUCGU GAGACAGUUC GGUCCCUAUC CGCUGCGCGC GCAGGAACAU UGAGAAGGGC UGUCCCUAGU ACGAG AGGA CCGGGACGGA CGAACCUCUG GUGUGCCAGU UGUUCUGCCA AGGGCAUGGC UGGUUGGCUA CGUUCGGGAG GGAUAA CCG CUGAAAGCAU CUAAGCGGGA AGCCUGCUUC GAGAUGAGUG UUCCCACCUC CUUGAGAGGG UAAGGCUCCC AGUAGAC GA CUGGGUUGAU AGGCCGGAUA UGGAAGCCCA GUGAUGGGUG GAGUUGACCG GUACUAAUAG GCCGAGGGCU UGUCCUC GENBANK: GENBANK: CP023696.1

-
Experimental details

-
Structure determination

Method	cryo EM
Processing	single particle reconstruction
Aggregation state	particle

-
Sample preparation

Buffer

pH: 7.5
Component:

Concentration	Formula	Name
50.0 mM	Tris-HCl	tris(hydroxymethyl)aminomethane-hydrochloric
20.0 mM	MgCH₃C₀₀₂	Magnesium acetate
50.0 mM	KCl	Potassium Chloride

Grid

Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. / Pretreatment - Atmosphere: OTHER / Pretreatment - Pressure: 0.026000000000000002 kPa

Vitrification

Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

Microscope	TFS GLACIOS
Temperature	Max: 77.0 K
Image recording	Film or detector model: FEI FALCON IV (4k x 4k) / Number grids imaged: 1 / Number real images: 4219 / Average exposure time: 7.94 sec. / Average electron dose: 50.0 e/Å²
Electron beam	Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron optics	C2 aperture diameter: 50.0 µm / Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 150000
Sample stage	Cooling holder cryogen: NITROGEN

+
Image processing

Particle selection	Number selected: 966178
CTF correction	Software - Name: cryoSPARC (ver. 4.6) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup model	Type of model: NONE
Final reconstruction	Applied symmetry - Point group: C₁ (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.13 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.6) / Number images used: 250126
Initial angle assignment	Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.6)
Final angle assignment	Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.6)
Final 3D classification	Number classes: 50 / Avg.num./class: 5791 / Software - Name: cryoSPARC (ver. 4.6) Details: The number of particles used for the final 2D classification is 289,553. The average particle in 50 classes is ~5,791.
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial model	Chain - Source name: AlphaFold / Chain - Initial model type: in silico model
Refinement	Space: REAL / Protocol: RIGID BODY FIT
Output model	PDB-9qt5: Structure of the 50S ribosomal subunit from the antibiotic-producing bacterium Streptomyces fradiae

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-Sample components

+Entire : Streptomyces fradiae ribosome

+Supramolecule #1: Streptomyces fradiae ribosome

+Macromolecule #1: Large ribosomal subunit protein uL16

+Macromolecule #3: Large ribosomal subunit protein uL2

+Macromolecule #4: Large ribosomal subunit protein uL3

+Macromolecule #5: Large ribosomal subunit protein uL4

+Macromolecule #6: Large ribosomal subunit protein uL5

+Macromolecule #7: Large ribosomal subunit protein uL6

+Macromolecule #8: Large ribosomal subunit protein bL9

+Macromolecule #9: Large ribosomal subunit protein uL13

+Macromolecule #10: Large ribosomal subunit protein uL14

+Macromolecule #11: Large ribosomal subunit protein uL18

+Macromolecule #12: Large ribosomal subunit protein uL15

+Macromolecule #13: Large ribosomal subunit protein bL19

+Macromolecule #14: Large ribosomal subunit protein bL20

+Macromolecule #15: Large ribosomal subunit protein bL21

+Macromolecule #16: Large ribosomal subunit protein uL22

+Macromolecule #17: Large ribosomal subunit protein uL23

+Macromolecule #18: Large ribosomal subunit protein uL24

+Macromolecule #19: Large ribosomal subunit protein bL25

+Macromolecule #20: Large ribosomal subunit protein bL27

+Macromolecule #21: Large ribosomal subunit protein bL28

+Macromolecule #22: Large ribosomal subunit protein uL29

+Macromolecule #23: Large ribosomal subunit protein uL30

+Macromolecule #24: Large ribosomal subunit protein bL32

+Macromolecule #25: Large ribosomal subunit protein bL33

+Macromolecule #26: Large ribosomal subunit protein bL34

+Macromolecule #27: Large ribosomal subunit protein bL35

+Macromolecule #28: Large ribosomal subunit protein bL36

+Macromolecule #29: Large ribosomal subunit protein bL17

+Macromolecule #2: 5S rRNA

+Macromolecule #30: 23S rRNA

-Experimental details

-Structure determination

-Sample preparation

-Electron microscopy

+Image processing

-Atomic model buiding 1

+About Yorodumi

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-Feb 9, 2022. New format data for meta-information of EMDB entries

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Movie

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Structure viewers

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Viewer log

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This page

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This web site

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Options

+
Open data

-
Basic information

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Structure visualization

-
Downloads & links

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Related structure data

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Links

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Map

Image control

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Supplemental data

-
Half map: #2

-
Half map: #1

-
Sample components

+
Entire : Streptomyces fradiae ribosome

+
Supramolecule #1: Streptomyces fradiae ribosome

+
Macromolecule #1: Large ribosomal subunit protein uL16

+
Macromolecule #3: Large ribosomal subunit protein uL2

+
Macromolecule #4: Large ribosomal subunit protein uL3

+
Macromolecule #5: Large ribosomal subunit protein uL4

+
Macromolecule #6: Large ribosomal subunit protein uL5

+
Macromolecule #7: Large ribosomal subunit protein uL6

+
Macromolecule #8: Large ribosomal subunit protein bL9

+
Macromolecule #9: Large ribosomal subunit protein uL13

+
Macromolecule #10: Large ribosomal subunit protein uL14

+
Macromolecule #11: Large ribosomal subunit protein uL18

+
Macromolecule #12: Large ribosomal subunit protein uL15

+
Macromolecule #13: Large ribosomal subunit protein bL19

+
Macromolecule #14: Large ribosomal subunit protein bL20

+
Macromolecule #15: Large ribosomal subunit protein bL21

+
Macromolecule #16: Large ribosomal subunit protein uL22

+
Macromolecule #17: Large ribosomal subunit protein uL23

+
Macromolecule #18: Large ribosomal subunit protein uL24

+
Macromolecule #19: Large ribosomal subunit protein bL25

+
Macromolecule #20: Large ribosomal subunit protein bL27

+
Macromolecule #21: Large ribosomal subunit protein bL28

+
Macromolecule #22: Large ribosomal subunit protein uL29

+
Macromolecule #23: Large ribosomal subunit protein uL30

+
Macromolecule #24: Large ribosomal subunit protein bL32

+
Macromolecule #25: Large ribosomal subunit protein bL33

+
Macromolecule #26: Large ribosomal subunit protein bL34

+
Macromolecule #27: Large ribosomal subunit protein bL35

+
Macromolecule #28: Large ribosomal subunit protein bL36

+
Macromolecule #29: Large ribosomal subunit protein bL17

+
Macromolecule #2: 5S rRNA

+
Macromolecule #30: 23S rRNA

-
Experimental details

-
Structure determination

-
Sample preparation

-
Electron microscopy

+
Image processing

-
Atomic model buiding 1

+
About Yorodumi

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News

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Feb 9, 2022. New format data for meta-information of EMDB entries

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Aug 12, 2020. Covid-19 info

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Mar 5, 2020. Novel coronavirus structure data

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Yorodumi