EMDB-53311: Cryo-EM map of SKM-70S ribosomal stalled complex in the major sta...

Basic information

Entry

Database: EMDB / ID: EMD-53311

• Title: Cryo-EM map of SKM-70S ribosomal stalled complex in the major state (vacant A-site, canon)
• Map data: Main map, postprocessed applying a mask on the whole 70S (j105) on the focus-refined 3Drefined map (j103)

Sample

• Complex: Cryo-EM structure of SKM-70S ribosomal stalled complex in the major state (vacant A-site, canon)
  • Protein or peptide: x 50 types
  • RNA: x 5 types
• Ligand: x 4 types

• Keywords: Antibiotics / 70S complex / Body closure / Accomodation / RIBOSOME

Function / homology

Function:

negative regulation of cytoplasmic translational initiation / transcription antitermination factor activity, RNA binding / ornithine decarboxylase inhibitor activity / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / positive regulation of ribosome biogenesis / RNA-binding transcription regulator activity / negative regulation of cytoplasmic translation / four-way junction DNA binding / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation / regulation of mRNA stability / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / positive regulation of RNA splicing / assembly of large subunit precursor of preribosome / cytosolic ribosome assembly / response to reactive oxygen species / regulation of DNA-templated transcription elongation / ribosome assembly / transcription elongation factor complex / transcription antitermination / DNA endonuclease activity / regulation of cell growth / translational initiation / DNA-templated transcription termination / response to radiation / maintenance of translational fidelity / mRNA 5'-UTR binding / regulation of translation / large ribosomal subunit / ribosome biogenesis / transferase activity / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / ribosomal large subunit assembly / 5S rRNA binding / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / hydrolase activity / response to antibiotic / negative regulation of DNA-templated transcription / mRNA binding / DNA binding / RNA binding / zinc ion binding / membrane / cytoplasm / cytosol

Domain/homology:

Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein S21 / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / : / Ribosomal protein L9, C-terminal domain superfamily / : / Ribosomal protein L17 signature. / : / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L28/L24 superfamily / : / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L9 / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L28 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / : / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L6, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / : / Ribosomal protein L5, bacterial-type / Ribosomal protein S5, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 / Ribosomal protein S9, bacterial/plastid / Ribosomal L28 family / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein L28/L24 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / Ribosomal protein L30, bacterial-type

Component:

Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL36A / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL25

• Biological species: Escherichia coli B (bacteria) / Streptococcus sanguinis (bacteria)
• Method: single particle reconstruction / cryo EM / Resolution: 2.26 Å
• Authors: Morici M / Corazza M / Safdari HA / Wilson DN

Funding support

United States, Canada, Germany, 4 items

Organization	Grant number	Country
National Institutes of Health/National Cancer Institute (NIH/NCI)	R35 GM127134	United States
National Institutes of Health/National Cancer Institute (NIH/NCI)	NIH R01 AI162961	United States
Canadian Institutes of Health Research (CIHR)	FDN148463	Canada
German Research Foundation (DFG)	WI3285/12-1	Germany

• Citation: Journal: Res Sq / Year: 2025; Title: Saskemycin, a potent and selective antimycobacterial agent targeting a unique site on the ribosome.; Authors: Gerard Wright / Michael Cook / Min Xu / Martino Morici / Dmitrii Travin / Wenliang Wang / Dorota Klepacki / Nandini Chhabra / Vishwas Rao / Henok Sahile / Dirk Hackenberger / Haaris Safdari / Max Berger / Martina Corazza / Austin Bond / Allison Guitor / Dominique Tertigas / Lijun Wang / Adam Schaenzer / Linda Ejim / Venkateswarlu Yarlagadda / James Gomez / Michael Surette / Yossef Av-Gay / Neeraj Dhar / Deborah Hung / Nora Vázquez-Laslop / Alexander Mankin / Daniel Wilson / ; Abstract: Tuberculosis is the deadliest bacterial disease on the planet. The months-long regimen of multiple antibiotics required to treat tuberculosis profoundly affects the microbiome and leads to the development of antimicrobial resistance. Furthermore, non-tuberculous mycobacterial infections pose an increasing clinical challenge. Consequently, there is a growing need for new narrow-spectrum mycobacteria-targeting antibiotics with different mechanisms of action. Here, we report the discovery and characterization of a natural glycolipid antibiotic, saskemycin (SKM), which demonstrates potent and highly selective activity against mycobacteria. Genome sequencing, chemical analysis, and isotope feeding strategies reveal the unique structure and biosynthetic origin of SKM. SKM binds to the small ribosomal subunit at a site not targeted by any of the clinically relevant antibiotics acting on the ribosome. Bound to the ribosome, SKM corrupts the decoding center in a unique way, preventing stable binding of aminoacyl-tRNA in the A site and inhibiting translation in a sequence context-specific manner. Self-resistance in the producing organism is conferred by methylation of a single 16S rRNA nucleotide by SasO and SasN rRNA methyltransferases. These enzymes are orthologs of the ubiquitous RsmC and SpoU methyltransferases found in most bacterial genera but absent in mycobacteria, rationalizing SKM's exquisite selectivity. The discovery of SKM provides an entry point for the development of selective, microbiome-sparing antimycobacterial antibiotics with a unique structure, binding site, and mechanism of action.

History

Deposition	Apr 1, 2025	-
Header (metadata) release	Feb 18, 2026	-
Map release	Feb 18, 2026	-
Update	Feb 18, 2026	-
Current status	Feb 18, 2026	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_53311.map.gz / Format: CCP4 / Size: 307.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Main map, postprocessed applying a mask on the whole 70S (j105) on the focus-refined 3Drefined map (j103)
• Voxel size: X=Y=Z: 0.832 Å

Density

Contour Level	By AUTHOR: 0.02
Minimum - Maximum	-0.08830086 - 0.19969368
Average (Standard dev.)	0.00045914482 (±0.0051904386)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 432 432 432 Spacing 432 432 432
Cell	A=B=C: 359.424 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_53311_msk_1.map

Mask #2

• File: emd_53311_msk_2.map

Additional map: 3Drefined cryo-EM map derived by focus refining on the 30S

• File: emd_53311_additional_1.map
• Annotation: 3Drefined cryo-EM map derived by focus refining on the 30S

Half map: Half map 2 used to produce the postprocessed map

• File: emd_53311_half_map_1.map
• Annotation: Half map 2 used to produce the postprocessed map

Half map: Half map 1 used to produce the postprocessed map

• File: emd_53311_half_map_2.map
• Annotation: Half map 1 used to produce the postprocessed map

Sample components

Entire : Cryo-EM structure of SKM-70S ribosomal stalled complex in the maj...

• Entire: Name: Cryo-EM structure of SKM-70S ribosomal stalled complex in the major state (vacant A-site, canon)

Components

• Complex: Cryo-EM structure of SKM-70S ribosomal stalled complex in the major state (vacant A-site, canon)
  • Protein or peptide: Small ribosomal subunit protein bS1
  • Protein or peptide: Large ribosomal subunit protein bL33
  • Protein or peptide: Large ribosomal subunit protein bL34
  • Protein or peptide: Large ribosomal subunit protein bL35
  • Protein or peptide: Large ribosomal subunit protein bL36A
  • Protein or peptide: Large ribosomal subunit protein bL31
  • RNA: E. coli 16S rRNA
  • Protein or peptide: Small ribosomal subunit protein uS2
  • Protein or peptide: Small ribosomal subunit protein uS3
  • Protein or peptide: Small ribosomal subunit protein uS4
  • Protein or peptide: Small ribosomal subunit protein uS5
  • Protein or peptide: Small ribosomal subunit protein bS6, fully modified isoform
  • Protein or peptide: Small ribosomal subunit protein uS7
  • Protein or peptide: Small ribosomal subunit protein uS8
  • Protein or peptide: Small ribosomal subunit protein uS9
  • Protein or peptide: Small ribosomal subunit protein uS10
  • Protein or peptide: Small ribosomal subunit protein uS11
  • Protein or peptide: Small ribosomal subunit protein uS12
  • Protein or peptide: Small ribosomal subunit protein uS13
  • Protein or peptide: Small ribosomal subunit protein uS14
  • Protein or peptide: Small ribosomal subunit protein uS15
  • Protein or peptide: Small ribosomal subunit protein bS16
  • Protein or peptide: Small ribosomal subunit protein uS17
  • Protein or peptide: Small ribosomal subunit protein bS18
  • Protein or peptide: Small ribosomal subunit protein uS19
  • Protein or peptide: Small ribosomal subunit protein bS20
  • Protein or peptide: Small ribosomal subunit protein bS21
  • RNA: ermBL mRNA transcript
  • RNA: P-site tRNA-fMet
  • RNA: E. coli 23S rRNA
  • RNA: E. coli 5S rRNA
  • Protein or peptide: Large ribosomal subunit protein uL2
  • Protein or peptide: Large ribosomal subunit protein uL3
  • Protein or peptide: Large ribosomal subunit protein uL4
  • Protein or peptide: Large ribosomal subunit protein uL5
  • Protein or peptide: Large ribosomal subunit protein uL6
  • Protein or peptide: Large ribosomal subunit protein bL9
  • Protein or peptide: Large ribosomal subunit protein uL13
  • Protein or peptide: Large ribosomal subunit protein uL14
  • Protein or peptide: Large ribosomal subunit protein uL15
  • Protein or peptide: Large ribosomal subunit protein uL16
  • Protein or peptide: Large ribosomal subunit protein bL17
  • Protein or peptide: Large ribosomal subunit protein uL18
  • Protein or peptide: Large ribosomal subunit protein bL19
  • Protein or peptide: Large ribosomal subunit protein bL20
  • Protein or peptide: Large ribosomal subunit protein bL21
  • Protein or peptide: Large ribosomal subunit protein uL22
  • Protein or peptide: Large ribosomal subunit protein uL23
  • Protein or peptide: Large ribosomal subunit protein uL24
  • Protein or peptide: Large ribosomal subunit protein bL25
  • Protein or peptide: Large ribosomal subunit protein bL27
  • Protein or peptide: Large ribosomal subunit protein bL28
  • Protein or peptide: Large ribosomal subunit protein uL29
  • Protein or peptide: Large ribosomal subunit protein uL30
  • Protein or peptide: Large ribosomal subunit protein bL32
• Ligand: ZINC ION
• Ligand: [(2~{S},3~{S},4~{R},5~{R},6~{S})-4-[(2~{S},3~{R},4~{S},5~{R},6~{S})-5-acetamido-6-(hydroxymethyl)-4-[(2~{R},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-4-[(2~{S},3~{R},4~{R},5~{R},6~{R})-6-(hydroxymethyl)-5-methoxy-3,4-bis(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-oxan-2-yl]oxy-6-carbamimidamido-2-(hydroxymethyl)-5-oxidanyl-oxan-3-yl] carbamate
• Ligand: MAGNESIUM ION
• Ligand: water

Supramolecule #1: Cryo-EM structure of SKM-70S ribosomal stalled complex in the maj...

• Supramolecule: Name: Cryo-EM structure of SKM-70S ribosomal stalled complex in the major state (vacant A-site, canon); type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#55
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 2.5 MDa

Macromolecule #1: Small ribosomal subunit protein bS1

• Macromolecule: Name: Small ribosomal subunit protein bS1 / type: protein_or_peptide / ID: 1 ; Details: Only a small fraction of the protein is visible and therefore modelled; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 51.449809 KDa

Sequence

String:

MTESFAQLFE ESLKEIETRP GSIVRGVVVA IDKDVVLVDA GLKSESAIPA EQFKNAQGEL EIQVGDEVDV ALDAVEDGFG ETLLSREKA KRHEAWITLE KAYEDAETVT GVINGKVKGG FTVELNGIRA FLPGSLVDVR PVRDTLHLEG KELEFKVIKL D QKRNNVVV SRRAVIESEN SAERDQLLEN LQEGMEVKGI VKNLTDYGAF VDLGGVDGLL HITDMAWKRV KHPSEIVNVG DE ITVKVLK FDRERTRVSL GLKQCKANPW QQFAETHNKG DRVEGKIKSI TDFGIFIGLD GGIDGLVHLS DISWNVAGEE AVR EYKKGD EIAAVVLQVD AERERISLGV KQLAEDPFNN WVALNKKGAI VTGKVTAVDA KGATVELADG VEGYLRASEA SRDR VEDAT LVLSVGDEVE AKFTGVDRKN RAISLSVRAK DEADEKDAIA TVNKQEDANF SNNAMAEAFK AAKGE

Macromolecule #2: Large ribosomal subunit protein bL33

• Macromolecule: Name: Large ribosomal subunit protein bL33 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 6.388631 KDa

Sequence

String:

MAKGIREKIK LVSSAGTGHF YTTTKNKRTK PEKLELKKFD PVVRQHVIYK EAKIK

UniProtKB: Large ribosomal subunit protein bL33

Macromolecule #3: Large ribosomal subunit protein bL34

• Macromolecule: Name: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 5.397463 KDa

Sequence

String:

MKRTFQPSVL KRNRSHGFRA RMATKNGRQV LARRRAKGRA RLTVSK

UniProtKB: Large ribosomal subunit protein bL34

Macromolecule #4: Large ribosomal subunit protein bL35

• Macromolecule: Name: Large ribosomal subunit protein bL35 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 7.313032 KDa

Sequence

String:

MPKIKTVRGA AKRFKKTGKG GFKHKHANLR HILTKKATKR KRHLRPKAMV SKGDLGLVIA CLPYA

UniProtKB: Large ribosomal subunit protein bL35

Macromolecule #5: Large ribosomal subunit protein bL36A

• Macromolecule: Name: Large ribosomal subunit protein bL36A / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 4.37739 KDa

Sequence

String:

MKVRASVKKL CRNCKIVKRD GVIRVICSAE PKHKQRQG

UniProtKB: Large ribosomal subunit protein bL36A

Macromolecule #6: Large ribosomal subunit protein bL31

• Macromolecule: Name: Large ribosomal subunit protein bL31 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 7.887117 KDa

Sequence

String:

MKKDIHPKYE EITASCSCGN VMKIRSTVGH DLNLDVCSKC HPFFTGKQRD VATGGRVDRF NKRFNIPGSK

UniProtKB: Large ribosomal subunit protein bL31

Macromolecule #8: Small ribosomal subunit protein uS2

• Macromolecule: Name: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 26.78167 KDa

Sequence

String:

MATVSMRDML KAGVHFGHQT RYWNPKMKPF IFGARNKVHI INLEKTVPMF NEALAELNKI ASRKGKILFV GTKRAASEAV KDAALSCDQ FFVNHRWLGG MLTNWKTVRQ SIKRLKDLET QSQDGTFDKL TKKEALMRTR ELEKLENSLG GIKDMGGLPD A LFVIDADH EHIAIKEANN LGIPVFAIVD TNSDPDGVDF VIPGNDDAIR AVTLYLGAVA ATVREGRSQD LASQAEESFV EA E

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #9: Small ribosomal subunit protein uS3

• Macromolecule: Name: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 26.031316 KDa

Sequence

String:

MGQKVHPNGI RLGIVKPWNS TWFANTKEFA DNLDSDFKVR QYLTKELAKA SVSRIVIERP AKSIRVTIHT ARPGIVIGKK GEDVEKLRK VVADIAGVPA QINIAEVRKP ELDAKLVADS ITSQLERRVM FRRAMKRAVQ NAMRLGAKGI KVEVSGRLGG A EIARTEWY REGRVPLHTL RADIDYNTSE AHTTYGVIGV KVWIFKGEIL GGMAAVEQPE KPAAQPKKQQ RKGRK

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #10: Small ribosomal subunit protein uS4

• Macromolecule: Name: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 23.514199 KDa

Sequence

String:

MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK VRRIYGVLER QFRNYYKEAA RLKGNTGEN LLALLEGRLD NVVYRMGFGA TRAEARQLVS HKAIMVNGRV VNIASYQVSP NDVVSIREKA KKQSRVKAAL E LAEQREKP TWLEVDAGKM EGTFKRKPER SDLSADINEH LIVELYSK

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #11: Small ribosomal subunit protein uS5

• Macromolecule: Name: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 17.629398 KDa

Sequence

String:

MAHIEKQAGE LQEKLIAVNR VSKTVKGGRI FSFTALTVVG DGNGRVGFGY GKAREVPAAI QKAMEKARRN MINVALNNGT LQHPVKGVH TGSRVFMQPA SEGTGIIAGG AMRAVLEVAG VHNVLAKAYG STNPINVVRA TIDGLENMNS PEMVAAKRGK S VEEILGK

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #12: Small ribosomal subunit protein bS6, fully modified isoform

• Macromolecule: Name: Small ribosomal subunit protein bS6, fully modified isoform; type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 15.727512 KDa

Sequence

String:

MRHYEIVFMV HPDQSEQVPG MIERYTAAIT GAEGKIHRLE DWGRRQLAYP INKLHKAHYV LMNVEAPQEV IDELETTFRF NDAVIRSMV MRTKHAVTEA SPMVKAKDER RERRDDFANE TADDAEAGDS EEEEEE

UniProtKB: Small ribosomal subunit protein bS6

Macromolecule #13: Small ribosomal subunit protein uS7

• Macromolecule: Name: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 20.055156 KDa

Sequence

String:

MPRRRVIGQR KILPDPKFGS ELLAKFVNIL MVDGKKSTAE SIVYSALETL AQRSGKSELE AFEVALENVR PTVEVKSRRV GGSTYQVPV EVRPVRRNAL AMRWIVEAAR KRGDKSMALR LANELSDAAE NKGTAVKKRE DVHRMAEANK AFAHYRWLSL R SFSHQAGA SSKQPALGYL N

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #14: Small ribosomal subunit protein uS8

• Macromolecule: Name: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 14.146557 KDa

Sequence

String:

MSMQDPIADM LTRIRNGQAA NKAAVTMPSS KLKVAIANVL KEEGFIEDFK VEGDTKPELE LTLKYFQGKA VVESIQRVSR PGLRIYKRK DELPKVMAGL GIAVVSTSKG VMTDRAARQA GLGGEIICYV A

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #15: Small ribosomal subunit protein uS9

• Macromolecule: Name: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 14.88627 KDa

Sequence

String:

MAENQYYGTG RRKSSAARVF IKPGNGKIVI NQRSLEQYFG RETARMVVRQ PLELVDMVEK LDLYITVKGG GISGQAGAIR HGITRALME YDESLRSELR KAGFVTRDAR QVERKKVGLR KARRRPQFSK R

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #16: Small ribosomal subunit protein uS10

• Macromolecule: Name: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 11.755597 KDa

Sequence

String:

MQNQRIRIRL KAFDHRLIDQ ATAEIVETAK RTGAQVRGPI PLPTRKERFT VLISPHVNKD ARDQYEIRTH LRLVDIVEPT EKTVDALMR LDLAAGVDVQ ISLG

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #17: Small ribosomal subunit protein uS11

• Macromolecule: Name: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 13.871959 KDa

Sequence

String:

MAKAPIRARK RVRKQVSDGV AHIHASFNNT IVTITDRQGN ALGWATAGGS GFRGSRKSTP FAAQVAAERC ADAVKEYGIK NLEVMVKGP GPGRESTIRA LNAAGFRITN ITDVTPIPH(IAS) GCRPPKKRRV

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #18: Small ribosomal subunit protein uS12

• Macromolecule: Name: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 13.814249 KDa

Sequence

String:

MATVNQLVRK PRARKVAKSN VPALEACPQK RGVCTRVYTT TPKKPNSALR KVCRVRLTNG FEVTSYIGGE GHNLQEHSVI LIRGGRVK(D2T) LPGVRYHTVR GALDCSGVKD RKQARSKYGV KRPKA

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #19: Small ribosomal subunit protein uS13

• Macromolecule: Name: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 13.128467 KDa

Sequence

String:

MARIAGINIP DHKHAVIALT SIYGVGKTRS KAILAAAGIA EDVKISELSE GQIDTLRDEV AKFVVEGDLR REISMSIKRL MDLGCYRGL RHRRGLPVRG QRTKTNARTR KGPRKPIKK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #20: Small ribosomal subunit protein uS14

• Macromolecule: Name: Small ribosomal subunit protein uS14 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 11.60656 KDa

Sequence

String:

MAKQSMKARE VKRVALADKY FAKRAELKAI ISDVNASDED RWNAVLKLQT LPRDSSPSRQ RNRCRQTGRP HGFLRKFGLS RIKVREAAM RGEIPGLKKA SW

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #21: Small ribosomal subunit protein uS15

• Macromolecule: Name: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 10.290816 KDa

Sequence

String:

MSLSTEATAK IVSEFGRDAN DTGSTEVQVA LLTAQINHLQ GHFAEHKKDH HSRRGLLRMV SQRRKLLDYL KRKDVARYTQ LIERLGLRR

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #22: Small ribosomal subunit protein bS16

• Macromolecule: Name: Small ribosomal subunit protein bS16 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 9.207572 KDa

Sequence

String:

MVTIRLARHG AKKRPFYQVV VADSRNARNG RFIERVGFFN PIASEKEEGT RLDLDRIAHW VGQGATISDR VAALIKEVNK AA

UniProtKB: Small ribosomal subunit protein bS16

Macromolecule #23: Small ribosomal subunit protein uS17

• Macromolecule: Name: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 9.724491 KDa

Sequence

String:

MTDKIRTLQG RVVSDKMEKS IVVAIERFVK HPIYGKFIKR TTKLHVHDEN NECGIGDVVE IRECRPLSKT KSWTLVRVVE KAVL

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #24: Small ribosomal subunit protein bS18

• Macromolecule: Name: Small ribosomal subunit protein bS18 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 9.005472 KDa

Sequence

String:

MARYFRRRKF CRFTAEGVQE IDYKDIATLK NYITESGKIV PSRITGTRAK YQRQLARAIK RARYLSLLPY TDRHQ

UniProtKB: Small ribosomal subunit protein bS18

Macromolecule #25: Small ribosomal subunit protein uS19

• Macromolecule: Name: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 10.455355 KDa

Sequence

String:

MPRSLKKGPF IDLHLLKKVE KAVESGDKKP LRTWSRRSTI FPNMIGLTIA VHNGRQHVPV FVTDEMVGHK LGEFAPTRTY RGHAADKKA KKK

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #26: Small ribosomal subunit protein bS20

• Macromolecule: Name: Small ribosomal subunit protein bS20 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 9.708464 KDa

Sequence

String:

MANIKSAKKR AIQSEKARKH NASRRSMMRT FIKKVYAAIE AGDKAAAQKA FNEMQPIVDR QAAKGLIHKN KAARHKANLT AQINKLA

UniProtKB: Small ribosomal subunit protein bS20

Macromolecule #27: Small ribosomal subunit protein bS21

• Macromolecule: Name: Small ribosomal subunit protein bS21 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 8.524039 KDa

Sequence

String:

MPVIKVRENE PFDVALRRFK RSCEKAGVLA EVRRREFYEK PTTERKRAKA SAVKRHAKKL ARENARRTRL Y

UniProtKB: Small ribosomal subunit protein bS21

Macromolecule #32: Large ribosomal subunit protein uL2

• Macromolecule: Name: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 29.923619 KDa

Sequence

String:

MAVVKCKPTS PGRRHVVKVV NPELHKGKPF APLLEKNSKS GGRNNNGRIT TRHIGGGHKQ AYRIVDFKRN KDGIPAVVER LEYDPNRSA NIALVLYKDG ERRYILAPKG LKAGDQIQSG VDAAIKPGNT LPMRNIPVGS TVHNVEMKPG KGGQLARSAG T YVQIVARD GAYVTLRLRS GEMRKVEADC RATLGEVGNA EHMLRVLGKA GAARWRGVRP TVRGTAMNPV DHPHGGGEGR NF GKHPVTP WGVQTKGKKT RSNKRTDKFI VRRRSK

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #33: Large ribosomal subunit protein uL3

• Macromolecule: Name: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 22.291562 KDa

Sequence

String:

MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV TKPEAGHFAK AGVEAGRGLW EFRLAEGEE FTVGQSISVE LFADVKKVDV TGTSKGKGFA GTVKRWNFRT QDATHGNSLS HRVPGSIGQN (MEQ)TPGKVF KG KKMAGQMGNE RVTVQSLDVV RVDAERNLLL VKGAVPGATG SDLIVKPAVK A

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #34: Large ribosomal subunit protein uL4

• Macromolecule: Name: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 22.121566 KDa

Sequence

String:

MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARP QDHSQKVNKK MYRGALKSIL SELVRQDRLI VVEKFSVEAP KTKLLAQKLK DMALEDVLII TGELDENLFL A ARNLHKVD VRDATGIDPV SLIAFDKVVM TADAVKQVEE MLA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #35: Large ribosomal subunit protein uL5

• Macromolecule: Name: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 20.333611 KDa

Sequence

String:

MAKLHDYYKD EVVKKLMTEF NYNSVMQVPR VEKITLNMGV GEAIADKKLL DNAAADLAAI SGQKPLITKA RKSVAGFKIR QGYPIGCKV TLRGERMWEF FERLITIAVP RIRDFRGLSA KSFDGRGNYS MGVREQIIFP EIDYDKVDRV RGLDITITTT A KSDEEGRA LLAAFDFPFR K

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #36: Large ribosomal subunit protein uL6

• Macromolecule: Name: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 18.932791 KDa

Sequence

String:

MSRVAKAPVV VPAGVDVKIN GQVITIKGKN GELTRTLNDA VEVKHADNTL TFGPRDGYAD GWAQAGTARA LLNSMVIGVT EGFTKKLQL VGVGYRAAVK GNVINLSLGF SHPVDHQLPA GITAECPTQT EIVLKGADKQ VIGQVAADLR AYRRPEPYKG K GVRYADEV VRTKEAKKK

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #37: Large ribosomal subunit protein bL9

• Macromolecule: Name: Large ribosomal subunit protein bL9 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 15.78902 KDa

Sequence

String:

MQVILLDKVA NLGSLGDQVN VKAGYARNFL VPQGKAVPAT KKNIEFFEAR RAELEAKLAE VLAAANARAE KINALETVTI ASKAGDEGK LFGSIGTRDI ADAVTAAGVE VAKSEVRLPN GVLRTTGEHE VSFQVHSEVF AKVIVNVVAE

UniProtKB: Large ribosomal subunit protein bL9

Macromolecule #38: Large ribosomal subunit protein uL13

• Macromolecule: Name: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 16.050606 KDa

Sequence

String:

MKTFTAKPET VKRDWYVVDA TGKTLGRLAT ELARRLRGKH KAEYTPHVDT GDYIIVLNAD KVAVTGNKRT DKVYYHHTGH IGGIKQATF EEMIARRPER VIEIAVKGML PKGPLGRAMF RKLKVYAGNE HNHAAQQPQV LDI

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #39: Large ribosomal subunit protein uL14

• Macromolecule: Name: Large ribosomal subunit protein uL14 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 13.565067 KDa

Sequence

String:

MIQEQTMLNV ADNSGARRVM CIKVLGGSHR RYAGVGDIIK ITIKEAIPRG KVKKGDVLKA VVVRTKKGVR RPDGSVIRFD GNACVLLNN NSEQPIGTRI FGPVTRELRS EKFMKIISLA PEVL

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #40: Large ribosomal subunit protein uL15

• Macromolecule: Name: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 15.008471 KDa

Sequence

String:

MRLNTLSPAE GSKKAGKRLG RGIGSGLGKT GGRGHKGQKS RSGGGVRRGF EGGQMPLYRR LPKFGFTSRK AAITAEIRLS DLAKVEGGV VDLNTLKAAN IIGIQIEFAK VILAGEVTTP VTVRGLRVTK GARAAIEAAG GKIEE

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #41: Large ribosomal subunit protein uL16

• Macromolecule: Name: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 15.343327 KDa

Sequence

String:

MLQPKRTKFR KMHKGRNRGL AQGTDVSFGS FGLKAVGRGR LTARQIEAAR RAMTRAVKRQ GKIWIRVFPD KPITEKPLAV (4D4)(MS6)GKGKGNV EYWVALIQPG KVLYEMDGVP EELAREAFKL AAAKLPIKTT FVTKTVM

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #42: Large ribosomal subunit protein bL17

• Macromolecule: Name: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 14.393657 KDa

Sequence

String:

MRHRKSGRQL NRNSSHRQAM FRNMAGSLVR HEIIKTTLPK AKELRRVVEP LITLAKTDSV ANRRLAFART RDNEIVAKLF NELGPRFAS RAGGYTRILK CGFRAGDNAP MAYIELVDRS EKAEAAAE

UniProtKB: Large ribosomal subunit protein bL17

Macromolecule #43: Large ribosomal subunit protein uL18

• Macromolecule: Name: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 12.794668 KDa

Sequence

String:

MDKKSARIRR ATRARRKLQE LGATRLVVHR TPRHIYAQVI APNGSEVLVA ASTVEKAIAE QLKYTGNKDA AAAVGKAVAE RALEKGIKD VSFDRSGFQY HGRVQALADA AREAGLQF

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #44: Large ribosomal subunit protein bL19

• Macromolecule: Name: Large ribosomal subunit protein bL19 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 13.159278 KDa

Sequence

String:

MSNIIKQLEQ EQMKQDVPSF RPGDTVEVKV WVVEGSKKRL QAFEGVVIAI RNRGLHSAFT VRKISNGEGV ERVFQTHSPV VDSISVKRR GAVRKAKLYY LRERTGKAAR IKERLN

UniProtKB: Large ribosomal subunit protein bL19

Macromolecule #45: Large ribosomal subunit protein bL20

• Macromolecule: Name: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 13.528024 KDa

Sequence

String:

MARVKRGVIA RARHKKILKQ AKGYYGARSR VYRVAFQAVI KAGQYAYRDR RQRKRQFRQL WIARINAAAR QNGISYSKFI NGLKKASVE IDRKILADIA VFDKVAFTAL VEKAKAALA

UniProtKB: Large ribosomal subunit protein bL20

Macromolecule #46: Large ribosomal subunit protein bL21

• Macromolecule: Name: Large ribosomal subunit protein bL21 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 11.586374 KDa

Sequence

String:

MYAVFQSGGK QHRVSEGQTV RLEKLDIATG ETVEFAEVLM IANGEEVKIG VPFVDGGVIK AEVVAHGRGE KVKIVKFRRR KHYRKQQGH RQWFTDVKIT GISA

UniProtKB: Large ribosomal subunit protein bL21

Macromolecule #47: Large ribosomal subunit protein uL22

• Macromolecule: Name: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 12.253359 KDa

Sequence

String:

METIAKHRHA RSSAQKVRLV ADLIRGKKVS QALDILTYTN KKAAVLVKKV LESAIANAEH NDGADIDDLK VTKIFVDEGP SMKRIMPRA KGRADRILKR TSHITVVVSD R

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #48: Large ribosomal subunit protein uL23

• Macromolecule: Name: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 11.22216 KDa

Sequence

String:

MIREERLLKV LRAPHVSEKA STAMEKSNTI VLKVAKDATK AEIKAAVQKL FEVEVEVVNT LVVKGKVKRH GQRIGRRSDW KKAYVTLKE GQNLDFVGGA E

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #49: Large ribosomal subunit protein uL24

• Macromolecule: Name: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 11.33925 KDa

Sequence

String:

MAAKIRRDDE VIVLTGKDKG KRGKVKNVLS SGKVIVEGIN LVKKHQKPVP ALNQPGGIVE KEAAIQVSNV AIFNAATGKA DRVGFRFED GKKVRFFKSN SETIK

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #50: Large ribosomal subunit protein bL25

• Macromolecule: Name: Large ribosomal subunit protein bL25 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 10.713465 KDa

Sequence

String:

MFTINAEVRK EQGKGASRRL RAANKFPAII YGGKEAPLAI ELDHDKVMNM QAKAEFYSEV LTIVVDGKEI KVKAQDVQRH PYKPKLQHI DFVRA

UniProtKB: Large ribosomal subunit protein bL25

Macromolecule #51: Large ribosomal subunit protein bL27

• Macromolecule: Name: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 9.14654 KDa

Sequence

String:

MAHKKAGGST RNGRDSEAKR LGVKRFGGES VLAGSIIVRQ RGTKFHAGAN VGCGRDHTLF AKADGKVKFE VKGPKNRKFI SIEAE

UniProtKB: Large ribosomal subunit protein bL27

Macromolecule #52: Large ribosomal subunit protein bL28

• Macromolecule: Name: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 9.027551 KDa

Sequence

String:

MSRVCQVTGK RPVTGNNRSH ALNATKRRFL PNLHSHRFWV ESEKRFVTLR VSAKGMRVID KKGIDTVLAE LRARGEKY

UniProtKB: Large ribosomal subunit protein bL28

Macromolecule #53: Large ribosomal subunit protein uL29

• Macromolecule: Name: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 7.286464 KDa

Sequence

String:

MKAKELREKS VEELNTELLN LLREQFNLRM QAASGQLQQS HLLKQVRRDV ARVKTLLNEK AGA

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #54: Large ribosomal subunit protein uL30

• Macromolecule: Name: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 6.55482 KDa

Sequence

String:

MAKTIKITQT RSAIGRLPKH KATLLGLGLR RIGHTVERED TPAIRGMINA VSFMVKVEE

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #55: Large ribosomal subunit protein bL32

• Macromolecule: Name: Large ribosomal subunit protein bL32 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 6.463445 KDa

Sequence

String:

MAVQQNKPTR SKRGMRRSHD ALTAVTSLSV DKTSGEKHLR HHITADGYYR GRKVIAK

UniProtKB: Large ribosomal subunit protein bL32

Macromolecule #7: E. coli 16S rRNA

• Macromolecule: Name: E. coli 16S rRNA / type: rna / ID: 7 / Number of copies: 1
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 499.55325 KDa

Sequence

String:

AAAUUGAAGA GUUUGAUCAU GGCUCAGAUU GAACGCUGGC GGCAGGCCUA ACACAUGCAA GUCGAACGGU AACAGGAAGA AGCUUGCUC UUUGCUGACG AGUGGCGGAC GGGUGAGUAA UGUCUGGGAA ACUGCCUGAU GGAGGGGGAU AACUACUGGA A ACGGUAGC UAAUACCGCA UAACGUCGCA AGACCAAAGA GGGGGACCUU CGGGCCUCUU GCCAUCGGAU GUGCCCAGAU GG GAUUAGC UAGUAGGUGG GGUAACGGCU CACCUAGGCG ACGAUCCCUA GCUGGUCUGA GAGGAUGACC AGCCACACUG GAA CUGAGA CACGGUCCAG ACUCCUACGG GAGGCAGCAG UGGGGAAUAU UGCACAAUGG GCGCAAGCCU GAUGCAGCCA UGCC GCGUG UAUGAAGAAG GCCUUCGGGU UGUAAAGUAC UUUCAGCGGG GAGGAAGGGA GUAAAGUUAA UACCUUUGCU CAUUG ACGU UACCCGCAGA AGAAGCACCG GCUAACUCCG (PSU)GCCAGCAGC C(G7M)CGGUAAUA CGGAGGGUGC AAGCGUUA A UCGGAAUUAC UGGGCGUAAA GCGCACGCAG GCGGUUUGUU AAGUCAGAUG UGAAAUCCCC GGGCUCAACC UGGGAACUG CAUCUGAUAC UGGCAAGCUU GAGUCUCGUA GAGGGGGGUA GAAUUCCAGG UGUAGCGGUG AAAUGCGUAG AGAUCUGGAG GAAUACCGG UGGCGAAGGC GGCCCCCUGG ACGAAGACUG ACGCUCAGGU GCGAAAGCGU GGGGAGCAAA CAGGAUUAGA U ACCCUGGU AGUCCACGCC GUAAACGAUG UCGACUUGGA GGUUGUGCCC UUGAGGCGUG GCUUCCGGAG CUAACGCGUU AA GUCGACC GCCUGGGGAG UACGGCCGCA AGGUUAAAAC UCAAAUGAAU UGACGGGGGC CCGCACAAGC GGUGGAGCAU GUG GUUUAA UUCGAU(2MG)(5MC)AA CGCGAAGAAC CUUACCUGGU CUUGACAUCC ACGGAAGUUU UCAGAGAUGA GAAUGU GCC UUCGGGAACC GUGAGACAGG UGCUGCAUGG CUGUCGUCAG CUCGUGUUGU GAAAUGUUGG GUUAAGUCCC GCAACGA GC GCAACCCUUA UCCUUUGUUG CCAGCGGUCC GGCCGGGAAC UCAAAGGAGA CUGCCAGUGA UAAACUGGAG GAAGGUGG G GAUGACGUCA AGUCAUCAUG GCCCUUACGA CCAGGGCUAC ACACGUGCUA CAAUGGCGCA UACAAAGAGA AGCGACCUC GCGAGAGCAA GCGGACCUCA UAAAGUGCGU CGUAGUCCGG AUUGGAGUCU GCAACUCGAC UCCAUGAAGU CGGAAUCGCU AGUAAUCGU GGAUCAGAAU GCCACGGUGA AUACGUUCCC GGGCCUUGUA CACACCG(4OC)CC GU(5MC)ACACCAU GGG AGUGGG UUGCAAAAGA AGUAGGUAGC UUAACCUUCG GGAGGGCGCU UACCACUUUG UGAUUCAUGA CUGGGGUGAA GUCG (UR3)AACA AGGUAACCGU AGG(2MG)G(MA6)(MA6)CCU GCGGUUGGAU CACCUCCUUA

Macromolecule #28: ermBL mRNA transcript

• Macromolecule: Name: ermBL mRNA transcript / type: rna / ID: 28 / Number of copies: 1
• Source (natural): Organism: Streptococcus sanguinis (bacteria)
• Molecular weight: Theoretical: 43.741992 KDa

Sequence

String:

UAAUACGACU CACUAUAGGG AGACUUAAGU AUAAGGAGGA AAAAAUAUGU UGGUAUUCCA AAUGCGUAAU GUAGAUAAAA CAUCUACUA UUUGAGUGAU AGAAUUCUAU CGUUAAUAAG CAAAAUUCAU UAUAACC

Macromolecule #29: P-site tRNA-fMet

• Macromolecule: Name: P-site tRNA-fMet / type: rna / ID: 29 / Number of copies: 1
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 23.581074 KDa

Sequence

String:

GCGGGGUGGA GCAGGGUAGC UCGUCGGGCU CAUAACCCGA AGGUCGUCGG UUCAAAUCCG GCCCCCGCAA CCA

Macromolecule #30: E. coli 23S rRNA

• Macromolecule: Name: E. coli 23S rRNA / type: rna / ID: 30 / Number of copies: 1
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 940.563812 KDa

Sequence

String:

GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGA UAUGAACCGU UAUAACCGGC GAUUUCCGAA UGGGGAAACC CAGUGUGUUU CGACACACUA UCAUUAACUG A AUCCAUAG GUUAAUGAGG CGAACCGGGG GAACUGAAAC AUCUAAGUAC CCCGAGGAAA AGAAAUCAAC CGAGAUUCCC CC AGUAGCG GCGAGCGAAC GGGGAGCAGC CCAGAGCCUG AAUCAGUGUG UGUGUUAGUG GAAGCGUCUG GAAAGGCGCG CGA UACAGG GUGACAGCCC CGUACACAAA AAUGCACAUG CUGUGAGCUC GAUGAGUAGG GCGGGACACG UGGUAUCCUG UCUG AAUAU GGGGGGACCA UCCUCCAAGG CUAAAUACUC CUGACUGACC GAUAGUGAAC CAGUACCGUG AGGGAAAGGC GAAAA GAAC CCCGGCGAGG GGAGUGAAAA AGAACCUGAA ACCGUGUACG UACAAGCAGU GGGAGCACGC UUAGGCGUGU GACUGC GUA CCUUUUGUAU AAUGGGUCAG CGACUUAUAU UCUGUAGCAA GGUUAACCGA AUAGGGGAGC CGAAGGGAAA CCGAGUC UU AACUGGGCGU UAAGUUGCAG GGUAUAGACC CGAAACCCGG UGAUCUAGCC AUGGGCAGGU UGAAGGUUGG GUAACACU A ACUGGAGGAC CGAACCGACU AAU(1MG)(PSU)(5MU)GAAA AAUUAGCGGA UGACUUGUGG CUGGGGGUGA AAGGCCA AU CAAACCGGGA GAUAGCUGGU UCUCCCCGAA AGCUAUUUAG GUAGCGCCUC GUGAAUUCAU CUCCGGGGGU AGAGCACU G UUUCGGCAAG GGGGUCAUCC CGACUUACCA ACCCGAUGCA AACUGCGAAU ACCGGAGAAU GUUAUCACGG GAGACACAC GGCGGG(PSU)GCU AACGUCCGUC GUGAAGAGGG AAACAACCCA GACCGCCAGC UAAGGUCCCA AAGUCAUGGU UAAGUG GGA AACGAUGUGG GAAGGCCCAG ACAGCCAGGA UGUUGGCUUA GAAGCAGCCA UCAUUUAAAG AAAGCGUAAU AGCUCAC UG GUCGAGUCGG CCUGCGCGGA AGAUGUAACG GGGCUAAACC AUGCACCGAA GCUGCGGCAG CGACGCGCGU UGUUGGGU A GGGGAGCGUU CUGUAAGCCU GCGAAGGUGU GCUGUGAGGC AUGCUGGAGG UAUCAGAAGU GCGAAUGCUG ACAUAAGUA ACGAUAAAGC GGGUGAAAAG CCCGCUCGCC GGAAGACCAA GGGUUCCUGU CCAACGUUAA UCGGGGCAGG GUGAGUCGAC CCCUAAGGC GAGGCCGAAA GGCGUAGUCG AUGGGAAACA GGUUAAUAUU CCUGUACUUG GUGUUACUGC GAAGGGGGGA C GGAGAAGG CUAUGUUGGC CGGGCGACGG UUGUCCCGGU UUAAGCGUGU AGGCUGGUUU UCCAGGCAAA UCCGGAAAAU CA AGGCUGA GGCGUGAUGA CGAGGCACUA CGGUGCUGAA GCAACAAAUG CCCUGCUUCC AGGAAAAGCC UCUAAGCAUC AGG UAACAU CAAAUCGUAC CCCAAACCGA CAC(6MZ)GGUGGU CAGGUAGAGA AUACCAAGGC GCUUGAGAGA ACUCGGGUGA AGGAACUAG GCAAAAUGGU GCCGUAACUU CGGGAGAAGG CACGCUGAUA UGUAGGUGAG GUCCCUCGCG GAUGGAGCUG A AAUCAGUC GAAGAUACCA GCUGGCUGCA ACUGUUUAUU AAAAACACAG CACUGUGCAA ACACGAAAGU GGACGUAUAC GG UGUGACG CCU(2MG)CCCGGU GCCGGAAGGU UAAUUGAUGG GGUUAGCGCA AGCGAAGCUC UUGAUCGAAG CCCCGGUAA ACGGCGGCCG (PSU)AAC(3TD)A(PSU)AAC GGUCCUAAGG UAGCGAAA(5MU)U CCUUGUCGGG UAAGUUCCGA C (5MC)UGCACGA AUGGCGUAAU GAUGGCCAGG CUGUCUCCAC CCGAGACUCA GUGAAAUUGA ACUCGCUGUG (6MZ)AGA UGCAG UGUACCCGCG GCAAGACGGA AAGACCCCGU (G7M)AACCUUUAC UAUAGCUUGA CACUGAACAU UGAGCCUUGA U GUGUAGGA UAGGUGGGAG GCUUUGAAGU GUGGACGCCA GUCUGCAUGG AGCCGACCUU GAAAUACCAC CCUUUAAUGU UU GAUGUUC UAACGUUGAC CCGUAAUCCG GGUUGCGGAC AGUGUCUGGU GGGUAGUUUG ACUG(OMG)GGCGG UCUCCUCCU AAAGAGUAAC GGAGGAGCAC GAAGGUUGGC UAAUCCUGGU CGGACAUCAG GAGGUUAGUG CAAUGGCAUA AGCCAGCUUG ACUGCGAGC GUGACGGCGC GAGCAGGUGC GAAAGCAGGU CAUAGUGAUC CGGUGGUUCU GAAUGGAAGG GCCAUCGCUC A ACGGAUAA AAGGUACUCC G(2MG)GGA(H2U)AACA GGC(PSU)GAUACC GCCCAAGAGU UCAUAUCGAC GGCGGUGUUU GGCA(OMC)CUCG (2MA)(PSU)GUCGGCUC AUCACAUCCU GGGGCUGAAG UAGGUCCCAA GGGUAUGGC(OMU) GUUCG CCAU UUAAAGUGGU ACGCGAGC(PSU)G GGUUUAGAAC GUCGUGAGAC AG(PSU)(PSU)CGGUCC CUAUCUGCCG UGGG CGCUG GAGAACUGAG GGGGGCUGCU CCUAGUACGA GAGGACCGGA GUGGACGCAU CACUGGUGUU CGGGUUGUCA UGCCA AUGG CACUGCCCGG UAGCUAAAUG CGGAAGAGAU AAGUGCUGAA AGCAUCUAAG CACGAAACUU GCCCCGAGAU GAGUUC UCC CUGACCCUUU AAGGGUCCUG AAGGAACGUU GAAGACGACG ACGUUGAUAG GCCGGGUGUG UAAGCGCAGC GAUGCGU UG AGCUAACCGG UACUAAUGAA CCGUGAGGCU UAACCUU

Macromolecule #31: E. coli 5S rRNA

• Macromolecule: Name: E. coli 5S rRNA / type: rna / ID: 31 / Number of copies: 1
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 38.79009 KDa

Sequence

String:

UGCCUGGCGG CCGUAGCGCG GUGGUCCCAC CUGACCCCAU GCCGAACUCA GAAGUGAAAC GCCGUAGCGC CGAUGGUAGU GUGGGGUCU CCCCAUGCGA GAGUAGGGAA CUGCCAGGCA U

GENBANK: GENBANK: CP049050.1

Macromolecule #56: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 56 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #57: [(2~{S},3~{S},4~{R},5~{R},6~{S})-4-[(2~{S},3~{R},4~{S},5~{R},6~{S...

• Macromolecule: Name: [(2~{S},3~{S},4~{R},5~{R},6~{S})-4-[(2~{S},3~{R},4~{S},5~{R},6~{S})-5-acetamido-6-(hydroxymethyl)-4-[(2~{R},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-4-[(2~{S},3~{R},4~{R},5~{R},6~{R})-6-(hydroxymethyl)-5-methoxy-3,4-bis(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-oxan-2-yl]oxy-6-carbamimidamido-2-(hydroxymethyl)-5-oxidanyl-oxan-3-yl] carbamate; type: ligand / ID: 57 / Number of copies: 1 / Formula: A1JA7
• Molecular weight: Theoretical: 805.736 Da

Macromolecule #58: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 58 / Number of copies: 312 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #59: water

• Macromolecule: Name: water / type: ligand / ID: 59 / Number of copies: 8128 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE-PROPANE

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 1.14 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 5.0 µm / Nominal defocus min: 0.5 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: INSILICO MODEL; In silico model: Molmap generated by a modified version of the pdb 7K00, containing no sample-specific component (deleted drugs, no tRNAs, no mRNA).
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.26 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 5) / Number images used: 343986
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD