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Yorodumi- EMDB-53137: Cryo-EM structure of the PlPVC1 baseplate, 6-fold symmetrized (C6... -
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Open data
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Basic information
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| Title | Cryo-EM structure of the PlPVC1 baseplate, 6-fold symmetrized (C6), in extended state | ||||||||||||||||||
Map data | Cryo-EM structure of the PlPVC baseplate, 6-fold symmetrized, in extended state | ||||||||||||||||||
Sample |
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Keywords | Contractile injection system / VIRUS LIKE PARTICLE | ||||||||||||||||||
| Function / homology | Function and homology information | ||||||||||||||||||
| Biological species | Photorhabdus luminescens (bacteria) | ||||||||||||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 2.68 Å | ||||||||||||||||||
Authors | Marin-Arraiza L / Taylor NMI | ||||||||||||||||||
| Funding support | Denmark, Spain, 5 items
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Citation | Journal: Nat Commun / Year: 2025Title: Structural characterization of an extracellular contractile injection system from Photorhabdus luminescens in extended and contracted states Authors: Marin-Arraiza L / Roa-Eguiara A / Pape T / Sofos N / Hendriks IA / Lund Nielsen M / Steiner-Rebrova EM / Taylor NMI | ||||||||||||||||||
| History |
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Structure visualization
| Supplemental images |
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Downloads & links
-EMDB archive
| Map data | emd_53137.map.gz | 661.7 MB | EMDB map data format | |
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| Header (meta data) | emd-53137-v30.xml emd-53137.xml | 28 KB 28 KB | Display Display | EMDB header |
| Images | emd_53137.png | 46.3 KB | ||
| Filedesc metadata | emd-53137.cif.gz | 8.5 KB | ||
| Others | emd_53137_half_map_1.map.gz emd_53137_half_map_2.map.gz | 1.2 GB 1.2 GB | ||
| Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-53137 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-53137 | HTTPS FTP |
-Validation report
| Summary document | emd_53137_validation.pdf.gz | 1 MB | Display | EMDB validaton report |
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| Full document | emd_53137_full_validation.pdf.gz | 1 MB | Display | |
| Data in XML | emd_53137_validation.xml.gz | 23 KB | Display | |
| Data in CIF | emd_53137_validation.cif.gz | 27.6 KB | Display | |
| Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-53137 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-53137 | HTTPS FTP |
-Related structure data
| Related structure data | ![]() 9qglMC ![]() 9qgmC ![]() 9qgnC ![]() 9qgoC ![]() 9qgpC M: atomic model generated by this map C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
| EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Map
| File | Download / File: emd_53137.map.gz / Format: CCP4 / Size: 1.3 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| Annotation | Cryo-EM structure of the PlPVC baseplate, 6-fold symmetrized, in extended state | ||||||||||||||||||||||||||||||||||||
| Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
| Voxel size | X=Y=Z: 1.2 Å | ||||||||||||||||||||||||||||||||||||
| Density |
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| Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
| Details | EMDB XML:
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-Supplemental data
-Half map: Cryo-EM structure of the PlPVC baseplate, 6-fold symmetrized,...
| File | emd_53137_half_map_1.map | ||||||||||||
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| Annotation | Cryo-EM structure of the PlPVC baseplate, 6-fold symmetrized, in extended state | ||||||||||||
| Projections & Slices |
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| Density Histograms |
-Half map: Cryo-EM structure of the PlPVC baseplate, 6-fold symmetrized,...
| File | emd_53137_half_map_2.map | ||||||||||||
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| Annotation | Cryo-EM structure of the PlPVC baseplate, 6-fold symmetrized, in extended state | ||||||||||||
| Projections & Slices |
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| Density Histograms |
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Sample components
+Entire : PlPVC baseplate (C6) in extended state
+Supramolecule #1: PlPVC baseplate (C6) in extended state
+Macromolecule #1: Phage tail protein
+Macromolecule #2: Phage tail sheath family protein
+Macromolecule #3: Phage tail protein
+Macromolecule #4: Contractile injection system tube protein N-terminal domain-conta...
+Macromolecule #5: Photorhabdus luminescens subsp. laumondii TTO1 complete genome se...
+Macromolecule #6: Photorhabdus luminescens subsp. laumondii TTO1 complete genome se...
+Macromolecule #7: Baseplate protein J-like domain-containing protein
+Macromolecule #8: Phage tail sheath family protein
+Macromolecule #9: Phage tail sheath family protein
-Experimental details
-Structure determination
| Method | cryo EM |
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Processing | single particle reconstruction |
| Aggregation state | particle |
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Sample preparation
| Buffer | pH: 7.4 |
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| Vitrification | Cryogen name: ETHANE |
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Electron microscopy
| Microscope | TFS KRIOS |
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| Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 41.0 e/Å2 |
| Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
| Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.6 µm |
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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About Yorodumi



Keywords
Photorhabdus luminescens (bacteria)
Authors
Denmark,
Spain, 5 items
Citation









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Processing
FIELD EMISSION GUN
