ジャーナル: EMBO J / 年: 2011 タイトル: Structural diversity of bacterial flagellar motors. 著者: Songye Chen / Morgan Beeby / Gavin E Murphy / Jared R Leadbetter / David R Hendrixson / Ariane Briegel / Zhuo Li / Jian Shi / Elitza I Tocheva / Axel Müller / Megan J Dobro / Grant J Jensen / 要旨: The bacterial flagellum is one of nature's most amazing and well-studied nanomachines. Its cell-wall-anchored motor uses chemical energy to rotate a microns-long filament and propel the bacterium ...The bacterial flagellum is one of nature's most amazing and well-studied nanomachines. Its cell-wall-anchored motor uses chemical energy to rotate a microns-long filament and propel the bacterium towards nutrients and away from toxins. While much is known about flagellar motors from certain model organisms, their diversity across the bacterial kingdom is less well characterized, allowing the occasional misrepresentation of the motor as an invariant, ideal machine. Here, we present an electron cryotomographical survey of flagellar motor architectures throughout the Bacteria. While a conserved structural core was observed in all 11 bacteria imaged, surprisingly novel and divergent structures as well as different symmetries were observed surrounding the core. Correlating the motor structures with the presence and absence of particular motor genes in each organism suggested the locations of five proteins involved in the export apparatus including FliI, whose position below the C-ring was confirmed by imaging a deletion strain. The combination of conserved and specially-adapted structures seen here sheds light on how this complex protein nanomachine has evolved to meet the needs of different species.
試料ホルダー: FEI Polara / 試料ホルダーモデル: GATAN HELIUM / Tilt series - Axis1 - Min angle: -60 ° / Tilt series - Axis1 - Max angle: 60 °
実験機器
モデル: Tecnai Polara / 画像提供: FEI Company
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画像解析
詳細
Average number of tilts used in the 3D reconstructions: 120. Average tomographic tilt angle increment: 1.
最終 再構成
アルゴリズム: OTHER / 解像度のタイプ: BY AUTHOR / 解像度: 59.0 Å / 解像度の算出法: FSC 0.5 CUT-OFF / ソフトウェア - 名称: IMOD, Bsoft 詳細: Final map is the C13-symmetrized average calculated from 94 individual sub-tomograms.