regulation of cytoplasmic translational initiation / dolichyl-diphosphooligosaccharide-protein glycotransferase / dolichyl-diphosphooligosaccharide-protein glycotransferase activity / preribosome / protein targeting to ER / oligosaccharyltransferase complex / protein N-linked glycosylation via asparagine / endoplasmic reticulum calcium ion homeostasis / protein transmembrane transporter activity / protein kinase activator activity ...regulation of cytoplasmic translational initiation / dolichyl-diphosphooligosaccharide-protein glycotransferase / dolichyl-diphosphooligosaccharide-protein glycotransferase activity / preribosome / protein targeting to ER / oligosaccharyltransferase complex / protein N-linked glycosylation via asparagine / endoplasmic reticulum calcium ion homeostasis / protein transmembrane transporter activity / protein kinase activator activity / protein targeting / post-translational protein modification / cellular response to amino acid starvation / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / intracellular protein transport / protein transport / large ribosomal subunit / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / calcium ion binding / endoplasmic reticulum membrane / endoplasmic reticulum / mitochondrion / RNA binding / zinc ion binding / metal ion binding / membrane / cytosol 類似検索 - 分子機能
SRP-independent targeting protein 3 / Phosphate transport (Pho88) / PAT complex subunit CCDC47 / PAT complex subunit CCDC47 / Translocon-associated protein (TRAP), alpha subunit / Translocon-associated protein (TRAP), alpha subunit / Gcn1, N-terminal / : / : / : ...SRP-independent targeting protein 3 / Phosphate transport (Pho88) / PAT complex subunit CCDC47 / PAT complex subunit CCDC47 / Translocon-associated protein (TRAP), alpha subunit / Translocon-associated protein (TRAP), alpha subunit / Gcn1, N-terminal / : / : / : / Generalcontrol nonderepressible 1 (Gcn1) N-terminal / Stalled ribosome sensor GCN1-like, HEAT repeats / Fungal GCN1-like, HEAT repeats / Stalled ribosome sensor GCN1 N-terminal / Stalled ribosome sensor GCN1-like, HEAT repeats / E-Z type HEAT repeats / PBS lyase HEAT-like repeat / Elongation factor 3/GNC1 HEAT repeat / EF3 N-terminal HEAT repeat / Centrosomal protein CEP104-like, TOG domain / : / STT3/PglB/AglB core domain / Parkin co-regulated protein / Parkin co-regulated protein / TOG domain / TOG / : / Oligosaccharyl transferase, STT3 subunit / Oligosaccharyl transferase STT3, N-terminal / : / : / Protein translocase SEC61 complex, gamma subunit / Protein translocase SecE domain superfamily / Translocon Sec61/SecY, plug domain / Plug domain of Sec61p / Protein secE/sec61-gamma signature. / Protein secY signature 1. / Protein secY signature 2. / SecE/Sec61-gamma subunits of protein translocation complex / Protein translocase complex, SecE/Sec61-gamma subunit / SecY/SEC61-alpha family / SecY domain superfamily / SecY conserved site / SecY / HEAT repeat profile. / HEAT, type 2 / Ribosomal protein L30e / Ribosomal protein L28e / : / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L19, eukaryotic / : / Ribosomal protein L10e / Ribosomal protein L6e signature. / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L44e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / : / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L30e signature 1. / Ribosomal protein L36e signature. / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal Protein L6, KOW domain / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site 類似検索 - ドメイン・相同性
Large ribosomal subunit protein uL3 / 60S ribosomal protein L26-like protein / Putative inorganic phosphate transporter protein / Ribosomal protein L15 / 60S ribosomal protein L27 / 60S ribosomal protein L6 / Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 / 60S ribosomal protein l9-like protein / 60S ribosomal protein l21-like protein / Ribosomal protein L37 ...Large ribosomal subunit protein uL3 / 60S ribosomal protein L26-like protein / Putative inorganic phosphate transporter protein / Ribosomal protein L15 / 60S ribosomal protein L27 / 60S ribosomal protein L6 / Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 / 60S ribosomal protein l9-like protein / 60S ribosomal protein l21-like protein / Ribosomal protein L37 / 60S ribosomal protein L14-like protein / 60S ribosomal protein L2-like protein / 60S ribosomal protein L25-like protein / Uncharacterized protein / 60S ribosomal protein L43-like protein / 60S ribosomal protein L32-like protein / 60S ribosomal protein L13 / Ribosomal protein L18-like protein / Ribosomal protein L19 / 60S ribosomal protein L8 / 60S ribosomal protein L36 / 60S ribosomal protein L44-like protein / 60S ribosomal protein L20 / 60S ribosomal protein l33-like protein / Putative 60S ribosomal protein / 60S ribosomal protein L22-like protein / Large ribosomal subunit protein uL18 / 60S ribosomal protein l30-like protein / 60S ribosomal protein L10-like protein / 60S ribosomal protein L4-like protein / Uncharacterized protein / 60S ribosomal protein l7-like protein / Ribosomal protein l34-like protein / Translocon-associated protein subunit alpha / Large ribosomal subunit protein uL22 / 60S ribosomal protein L16-like protein / Translocon Sec61/SecY plug domain-containing protein / Putative 60S ribosomal protein / Large ribosomal subunit protein uL5 / 60S ribosomal protein l23-like protein / Small ribosomal subunit protein eS32A / Large ribosomal subunit protein eL39 / 60S ribosomal protein L29 類似検索 - 構成要素
H2020 Marie Curie Actions of the European Commission
HORIZON-MSCA-2022-PF-01; Project ID: 101107937
European Union
Max Planck Society
ドイツ
German Research Foundation (DFG)
SFB 1507/P16 (project-ID 450648163)
ドイツ
引用
ジャーナル: Nat Commun / 年: 2025 タイトル: SND3 is the membrane insertase within a distinct SEC61 translocon complex. 著者: Tzu-Jing Yang / Saumyak Mukherjee / Julian D Langer / Gerhard Hummer / Melanie A McDowell / 要旨: During the biogenesis of most eukaryotic integral membrane proteins (IMPs), transmembrane domains are inserted into the endoplasmic reticulum membrane by a dedicated insertase or the SEC61 translocon. ...During the biogenesis of most eukaryotic integral membrane proteins (IMPs), transmembrane domains are inserted into the endoplasmic reticulum membrane by a dedicated insertase or the SEC61 translocon. The SRP-independent (SND) pathway is the least understood route into the membrane, despite catering for a broad range of IMP types. Here, we show that Chaetomium thermophilum SND3 is a membrane insertase with an atypical fold. We further present a cryo-electron microscopy structure of a ribosome-associated SND3 translocon complex involved in co-translational IMP insertion. The structure reveals that the SND3 translocon additionally comprises the complete SEC61 translocon, CCDC47 and TRAPɑ. Here, the SEC61β N-terminus works together with CCDC47 to prevent substrate access to the translocon. Instead, molecular dynamics simulations show that SND3 disrupts the lipid bilayer to promote IMP insertion via its membrane-embedded hydrophilic groove. Structural and sequence comparisons indicate that the SND3 translocon is a distinct multipass translocon in fungi, euglenozoan parasites and other eukaryotic taxa.