EMDB-52656: Cryo-EM structure of Chaetomium thermophilum ribosome-bound SND3 ...

Basic information

Entry

Database: EMDB / ID: EMD-52656

• Title: Cryo-EM structure of Chaetomium thermophilum ribosome-bound SND3 translocon
• Map data: Cryo-EM structure of Chaetomium thermophilum ribosome-bound SND3 translocon complex

Sample

• Complex: ribosome-bound SND3 translocon
  • Protein or peptide: x 47 types
  • RNA: x 3 types
• Ligand: x 1 types

• Keywords: membrane protein biogenesis / SND3 / ribosome / co-translation translocation / insertase

Function / homology

Function:

dolichyl-diphosphooligosaccharide-protein glycotransferase / dolichyl-diphosphooligosaccharide-protein glycotransferase activity / preribosome / protein targeting to ER / protein N-linked glycosylation via asparagine / endoplasmic reticulum calcium ion homeostasis / protein kinase regulator activity / protein transmembrane transporter activity / protein targeting / endomembrane system / post-translational protein modification / cellular response to amino acid starvation / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / intracellular protein transport / protein transport / ribosome biogenesis / large ribosomal subunit / regulation of translation / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / calcium ion binding / endoplasmic reticulum membrane / endoplasmic reticulum / mitochondrion / RNA binding / zinc ion binding / metal ion binding / membrane / cytosol

Domain/homology:

SRP-independent targeting protein 3 / Phosphate transport (Pho88) / PAT complex subunit CCDC47 / PAT complex subunit CCDC47 / Translocon-associated protein (TRAP), alpha subunit / Translocon-associated protein (TRAP), alpha subunit / Gcn1, N-terminal / : / : / Generalcontrol nonderepressible 1 (Gcn1) N-terminal / Stalled ribosome sensor GCN1-like, HEAT repeats / Fungal GCN1-like, HEAT repeats / Stalled ribosome sensor GCN1 N-terminal / E-Z type HEAT repeats / PBS lyase HEAT-like repeat / Elongation factor 3/GNC1 HEAT repeat / EF3 N-terminal HEAT repeat / Centrosomal protein CEP104-like, TOG domain / : / STT3/PglB/AglB core domain / Parkin co-regulated protein / Parkin co-regulated protein / TOG domain / TOG / : / Oligosaccharyl transferase, STT3 subunit / Oligosaccharyl transferase STT3, N-terminal / Protein translocase SEC61 complex, gamma subunit / Protein translocase SecE domain superfamily / Translocon Sec61/SecY, plug domain / Plug domain of Sec61p / Protein secE/sec61-gamma signature. / Protein secY signature 1. / Protein secY signature 2. / SecE/Sec61-gamma subunits of protein translocation complex / Protein translocase complex, SecE/Sec61-gamma subunit / SecY/SEC61-alpha family / SecY domain superfamily / SecY conserved site / SecY / HEAT repeat profile. / HEAT, type 2 / Ribosomal protein L30e / Ribosomal protein L28e / : / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L19, eukaryotic / : / Ribosomal protein L10e / Ribosomal protein L6e signature. / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L44e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / : / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L30e signature 1. / Ribosomal protein L36e signature. / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal Protein L6, KOW domain / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site / Ribosomal protein L6e / : / Ribosomal protein L34Ae / Ribosomal protein L34e

Component:

Large ribosomal subunit protein uL3 / 60S ribosomal protein L26-like protein / Putative inorganic phosphate transporter protein / Ribosomal protein L15 / 60S ribosomal protein L27 / 60S ribosomal protein L6 / Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 / 60S ribosomal protein l9-like protein / 60S ribosomal protein l21-like protein / Ribosomal protein L37 / 60S ribosomal protein L14-like protein / 60S ribosomal protein L2-like protein / 60S ribosomal protein L25-like protein / Uncharacterized protein / 60S ribosomal protein L43-like protein / 60S ribosomal protein L32-like protein / 60S ribosomal protein L13 / Ribosomal protein L18-like protein / Ribosomal protein L19 / 60S ribosomal protein L8 / 60S ribosomal protein L36 / 60S ribosomal protein L44-like protein / 60S ribosomal protein L20 / 60S ribosomal protein l33-like protein / Putative 60S ribosomal protein / 60S ribosomal protein L22-like protein / Large ribosomal subunit protein uL18 / 60S ribosomal protein l30-like protein / 60S ribosomal protein L10-like protein / 60S ribosomal protein L4-like protein / Uncharacterized protein / 60S ribosomal protein l7-like protein / Ribosomal protein l34-like protein / Translocon-associated protein subunit alpha / Large ribosomal subunit protein uL22 / 60S ribosomal protein L16-like protein / Translocon Sec61/SecY plug domain-containing protein / Putative 60S ribosomal protein / Large ribosomal subunit protein uL5 / 60S ribosomal protein l23-like protein / Small ribosomal subunit protein eS32A / Large ribosomal subunit protein eL39 / 60S ribosomal protein L29

• Biological species: Thermochaetoides thermophila DSM 1495 (fungus)
• Method: single particle reconstruction / cryo EM / Resolution: 2.2 Å
• Authors: Yang TJ / McDowell MA

Funding support

European Union, Germany, 3 items

Organization	Grant number	Country
H2020 Marie Curie Actions of the European Commission	HORIZON-MSCA-2022-PF-01; Project ID: 101107937	European Union
Max Planck Society		Germany
German Research Foundation (DFG)	SFB 1507/P16 (project-ID 450648163)	Germany

• Citation: Journal: Nat Commun / Year: 2025; Title: SND3 is the membrane insertase within a distinct SEC61 translocon complex.; Authors: Tzu-Jing Yang / Saumyak Mukherjee / Julian D Langer / Gerhard Hummer / Melanie A McDowell / ; Abstract: During the biogenesis of most eukaryotic integral membrane proteins (IMPs), transmembrane domains are inserted into the endoplasmic reticulum membrane by a dedicated insertase or the SEC61 translocon. The SRP-independent (SND) pathway is the least understood route into the membrane, despite catering for a broad range of IMP types. Here, we show that Chaetomium thermophilum SND3 is a membrane insertase with an atypical fold. We further present a cryo-electron microscopy structure of a ribosome-associated SND3 translocon complex involved in co-translational IMP insertion. The structure reveals that the SND3 translocon additionally comprises the complete SEC61 translocon, CCDC47 and TRAPɑ. Here, the SEC61β N-terminus works together with CCDC47 to prevent substrate access to the translocon. Instead, molecular dynamics simulations show that SND3 disrupts the lipid bilayer to promote IMP insertion via its membrane-embedded hydrophilic groove. Structural and sequence comparisons indicate that the SND3 translocon is a distinct multipass translocon in fungi, euglenozoan parasites and other eukaryotic taxa.

History

Deposition	Jan 31, 2025	-
Header (metadata) release	Nov 12, 2025	-
Map release	Nov 12, 2025	-
Update	Nov 12, 2025	-
Current status	Nov 12, 2025	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_52656.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Cryo-EM structure of Chaetomium thermophilum ribosome-bound SND3 translocon complex
• Voxel size: X=Y=Z: 1.04625 Å

Density

Contour Level	By AUTHOR: 3.0
Minimum - Maximum	-12.158384 - 40.186717999999999
Average (Standard dev.)	-0.000000000004269 (±1.0)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 512 512 512 Spacing 512 512 512
Cell	A=B=C: 535.68 Å α=β=γ: 90.0 °

Supplemental data

Additional map: Unsharpened map of Chaetomium thermophilum ribosome-bound SND3 translocon...

• File: emd_52656_additional_1.map
• Annotation: Unsharpened map of Chaetomium thermophilum ribosome-bound SND3 translocon complex

Half map: Half-map (A) of Chaetomium thermophilum ribosome-bound SND3 translocon...

• File: emd_52656_half_map_1.map
• Annotation: Half-map (A) of Chaetomium thermophilum ribosome-bound SND3 translocon complex

Half map: Half-map (B) of Chaetomium thermophilum ribosome-bound SND3 translocon...

• File: emd_52656_half_map_2.map
• Annotation: Half-map (B) of Chaetomium thermophilum ribosome-bound SND3 translocon complex

Sample components

Entire : ribosome-bound SND3 translocon

• Entire: Name: ribosome-bound SND3 translocon

Components

• Complex: ribosome-bound SND3 translocon
  • Protein or peptide: Translocon Sec61/SecY plug domain-containing protein
  • Protein or peptide: SEC61 beta subunit
  • Protein or peptide: SEC61 gamma subunit
  • Protein or peptide: CCDC47
  • Protein or peptide: Putative inorganic phosphate transporter protein
  • Protein or peptide: Translocon-associated protein subunit alpha
  • Protein or peptide: 60S ribosomal protein L2-like protein
  • Protein or peptide: 60S ribosomal protein L3-like protein
  • Protein or peptide: 60S ribosomal protein L4-like protein
  • Protein or peptide: 60S ribosomal protein l5-like protein
  • Protein or peptide: 60S ribosomal protein L6
  • Protein or peptide: 60S ribosomal protein l7-like protein
  • Protein or peptide: 60S ribosomal protein L8
  • Protein or peptide: 60S ribosomal protein l9-like protein
  • Protein or peptide: 60S ribosomal protein L10-like protein
  • Protein or peptide: Putative ribosomal protein
  • Protein or peptide: 60S ribosomal protein L13
  • Protein or peptide: 60S ribosomal protein L14-like protein
  • Protein or peptide: Ribosomal protein L15
  • Protein or peptide: 60S ribosomal protein L16-like protein
  • Protein or peptide: 60S ribosomal protein l17-like protein
  • Protein or peptide: Ribosomal protein L18-like protein
  • Protein or peptide: Ribosomal protein L19
  • Protein or peptide: 60S ribosomal protein L20
  • Protein or peptide: 60S ribosomal protein l21-like protein
  • Protein or peptide: 60S ribosomal protein L22-like protein
  • Protein or peptide: 60S ribosomal protein l23-like protein
  • Protein or peptide: 60S ribosomal protein L25-like protein
  • Protein or peptide: 60S ribosomal protein L26-like protein
  • Protein or peptide: 60S ribosomal protein L27
  • Protein or peptide: 60S ribosomal protein L28-like protein
  • Protein or peptide: 60S ribosomal protein L29
  • Protein or peptide: 60S ribosomal protein l30-like protein
  • Protein or peptide: Putative 60S ribosomal protein
  • Protein or peptide: 60S ribosomal protein L32-like protein
  • Protein or peptide: 60S ribosomal protein l33-like protein
  • Protein or peptide: Ribosomal protein l34-like protein
  • Protein or peptide: Dolichyl-diphosphooligosaccharide--protein glycotransferase
  • Protein or peptide: 60S ribosomal protein L36
  • Protein or peptide: Ribosomal protein L37
  • Protein or peptide: 60S ribosomal protein L38-like protein
  • Protein or peptide: Ribosomal protein eL39
  • Protein or peptide: Putative ribosomal protein
  • Protein or peptide: 60S ribosomal protein L41-A
  • Protein or peptide: 60S ribosomal protein L44-like protein
  • Protein or peptide: 60S ribosomal protein L43-like protein
  • Protein or peptide: Putative 60S ribosomal protein
  • RNA: 26S rRNA
  • RNA: 5.8S rRNA
  • RNA: 5S rRNA
• Ligand: MAGNESIUM ION

Supramolecule #1: ribosome-bound SND3 translocon

• Supramolecule: Name: ribosome-bound SND3 translocon / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#50
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)

Macromolecule #1: Translocon Sec61/SecY plug domain-containing protein

• Macromolecule: Name: Translocon Sec61/SecY plug domain-containing protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 52.32232 KDa

Sequence

String:

MSSLRFLDLV KPFVPFLPEV QQPESKIPFN QKLMWTGLTL LIFLVMSQMP LYGIVSSDSS DPLYWLRMMM ASNRGTLMEL GITPIISSG MVFQLLAGTH MIDVNLDLKS DRELYQSAQK LFALILSVGT ATVYVFTGLY GPPSELGAGI VFLLILQLVL A GMIVILLD ELLQKGYGLG SGISLFIATN ICESIMWKAF SPTSINTGRG PEYEGAVIAL FHLLMTWPNK QRALQEAFYR QN LPNIMNL LATIVVFAAV IYLQGFRVEI PVKSSRQRGA RGSYPVRLFY TSNMPIMLQS TLSSNIFLIS QMLYSRFSDN LLV RLFGVW EAKDGSSQLS AVSGLVYYMS PPLNFKEALL DPIHTFVYIA YMLTACAIFS KTWIEVSGSS PRDVAKQLKD QGLV MAGHR DQSMYKELKR VIPTAAAFGG ACIGALSVAS DLMGALGSGT GTLLAVTIIY GYFEIAAKEG DLAGMKGMIM G

UniProtKB: Translocon Sec61/SecY plug domain-containing protein

Macromolecule #2: SEC61 beta subunit

• Macromolecule: Name: SEC61 beta subunit / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 12.552324 KDa

Sequence

String:

MASPRATSPA PAGEKGSATG ASTSASPGVL GGPRGSSPPP PGGARTAIRR RAAADQKERL ANQRPNSTRA AGAGGSSNTM LKLYTDESP GLKVDPVVVL VLSLVFIFSV VALHVIAKIT RKFSS

Macromolecule #3: SEC61 gamma subunit

• Macromolecule: Name: SEC61 gamma subunit / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 7.81819 KDa

Sequence

String:

MADQIQELLD VPREFLKDGI QFINRAQKPD RREFIKISQA VGVGFLIMGA VGYFVKLIHV PLNNILVGGA

UniProtKB: Uncharacterized protein

Macromolecule #4: CCDC47

• Macromolecule: Name: CCDC47 / type: protein_or_peptide / ID: 4 ; Details: genome engineered CCDC47 (internal FLAG-tag after the residue T60); Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 51.723922 KDa
• Recombinant expression: Organism: Thermochaetoides thermophila DSM 1495 (fungus)

Sequence

String:

MANVLNNLFG GGKPSVSPDP LVAGDDFADF SQAAEPSPVT VQPLATTPSA VPLQPARPYT DYKDDDDKKW YRLHERYTWS DFKSEGIIL CIISVFIILH VIGASLNRRK AKKWIRAHAA PLAAEFAVVG YSGIPKNVSD KKGEELVKAL QDSNIAQGDN L IKERSLFE FATYATGRAN VAFLEVKIAL TKRFNPLTAF FESVLGFFFE SGPEVGDRVE ATLYPFDGKE ADVVPDFPGA AE LRSKDPK STYDNFVWAI VHKECMKKAR NDRYDLSLTY TKDHAKLPNW LAVMSESAEI TDALLTPELI KAAEAAGDLF EYL VVTDQP EDKPKTLNET RPRKRVILKY RVPSNDDYTS LLPIFEYFLR MPDHLVQVAH FRPEVLRKVK VVRDEEIRKI QKAE EESKA EERAQEREKA KKAKRDQELS QLDAKAQKKY LEREREKELK RSMKKATIRA

UniProtKB: Uncharacterized protein

Macromolecule #5: Putative inorganic phosphate transporter protein

• Macromolecule: Name: Putative inorganic phosphate transporter protein / type: protein_or_peptide / ID: 5 ; Details: Putative inorganic phosphate transporter protein with C-terminal TwinStrep-tag; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 24.180205 KDa
• Recombinant expression: Organism: Thermochaetoides thermophila DSM 1495 (fungus)

Sequence

String:

MALSPQITNL IIIFGMMQVS KKIPFDDPNV LNMCRAAYLA SNLIILCISL YIKSVVDKKK DMTTLKYIEP APPGSSEEGK LVTTTVHAY DVEQVKILIK SQLFGIGMMA FMHLYLKYTN PLLIQSIIPL KGALESNMAK IHLWGMPATG DLKRPFKQPG G LLGGLFGQ SVQADKKAVE AAERAGRGGV KEEGSGSAWS HPQFEKGGGS GGGSGGSAWS HPQFEK

UniProtKB: Putative inorganic phosphate transporter protein

Macromolecule #6: Translocon-associated protein subunit alpha

• Macromolecule: Name: Translocon-associated protein subunit alpha / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 29.964066 KDa

Sequence

String:

MVNFKWSALA LLALRVAAVF AQEESADAEP GVLKEVSPDE LKVSIDTTFP DADIFGVKLV NGRPTKAIIE ITNHEEVPVD VAFIGGILK TTKPLPEDAP HYASIVRNLT AVRYGVQVPA GEKHQLPYQF VLDMMPQDVN LVLSAVITNA AAGKIYEVEA H SGPASIVD PPTSIFDPQI IFLYLFLTAF FSGIAYWVYK TWLEQFFPQA KPSRAAGPAK GKKARRAGGA TSDAEPVSGS ES AGGNTSG AGEKEYDESW IPAHHINRPV ARKVKGGSKS K

UniProtKB: Translocon-associated protein subunit alpha

Macromolecule #7: 60S ribosomal protein L2-like protein

• Macromolecule: Name: 60S ribosomal protein L2-like protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 27.598875 KDa

Sequence

String:

MGRVIRNQRK GRGSVFTAHT RLRKAPAKFR PLDYAERHGY IRGIVKEIIH DPGRGAPLAR VVFRSPYKYK QITETFIANE GMYTGQFIY AGKNAALTVG NILPLSSVPE GTVVSNVEEK PGDRGALGRT SGNYVTVVGH NPDEGKTRIK LPSGAKKVVP S SSRGMIGI VAGGGRTDKP LLKASRAKHK FAVKRNRWPK TRGVAMNPVD HPHGGGNHQH IGKASTISRY AAQGQKAGLI AA RRTGLLR GTQKTKE

UniProtKB: 60S ribosomal protein L2-like protein

Macromolecule #8: 60S ribosomal protein L3-like protein

• Macromolecule: Name: 60S ribosomal protein L3-like protein / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 44.456855 KDa

Sequence

String:

MSHRKYEAPR HGSLAFLPRK RAARHRGRVK SFPKDDPKKP VHLTAAMGYK AGMTTIVRDL DRPGAKAHKK EVVEAVTIID CPPMVVVGL VGYIETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYAKKYAE NNGASITREL ERIKKYCTVV R VLAHTQIR KTPLKQKKAH LMEIQINGGS VADKVEFGRS LFEKPVTIDT IFEKDEMIDV IAVTKGHGFV GVTARWGTKK LP RKTHKGL RKVACIGAWH PSHVQWTVAR AGQMGYHHRT SVNHKIYRIG KGDDEANAST ETDLTKKKIT PMGGFVRYGE VNN DYVMIK GSVPGVKKRI MTLRKSLFTH TSRKALEKVE LKWIDTSSEF GHGAFQTAAE KKQFMGTLKK DLQTSA

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #9: 60S ribosomal protein L4-like protein

• Macromolecule: Name: 60S ribosomal protein L4-like protein / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 39.439938 KDa

Sequence

String:

MASRPTVTVF GADGKPTGAT EVLPKVFSAP IRPDIVKHVH TGMAKNKRQP YAVSEKAGHQ TSAESWGTGR AVARIPRVSG GGTHRAGQG AFGNMCRSGR MFAPTKIWRK WHVKINQGQK RFATASALAA SAVAPLLMAR GHQVSTVPEV PLVVDSAAVA G DAVAKTAA AYKLLKAIGA GPDVEKVKKS KKLRAGKGKM RGRRHRQRRG PLIVYSPEHD GKELVKGFRN IPGVETCPVD AL NLLQLAP GGHLGRFIVW TSAAIKQLDA VYESKKGFFL PANIVSQADL SRLINSTEIQ SVLRAPKGEA RTKRACVQKK NPL RNKQIM LRLNPYASTF AKEKLGEVKA EEGKPPKVPA SFKELLHEA

UniProtKB: 60S ribosomal protein L4-like protein

Macromolecule #10: 60S ribosomal protein l5-like protein

• Macromolecule: Name: 60S ribosomal protein l5-like protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 35.034605 KDa

Sequence

String:

MAFHKLVKNS AYYSRFQTKF KRRRQGKTDY YARKRLITQA KNKYNAPKYR LVVRFTNRDI ITQMVTSEIN GDKIFAAAYS HELRAYGIN HGLTNWAAAY ATGLLLARRV LAKLGLDKTF TGVEEPNGEY TLTEAAETED GERRPFKAIL DVGLARTSTG A RVFGVMKG ASDGGIFIPH SENRFPGYDI ETEELDTEVL KKYIYGGHVA EYMETLADDD EERYKSQFVK YIEDDVEADS LE ELYAEAH KQIRADPFRK YVSDAPKKSK EEWKAESLKY KKAKLSREER KARVEAKIKQ LLAEQDE

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #11: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 22.132994 KDa

Sequence

String:

MSAAPTTKTF GKGTRTVPAP SEKAQKWYPA EDEAQPKKVR KAVRPWTPRK SLQPGTVLIL LAGRFRGKRV VLLKCLDQGV LLVTGPFKI NGVPLRRVNA RYVIATSVKV DLTGVDQAKI DEVAQPKYFT AEKAKEKASE EAFFKQGEKP QKKPVSSTRA A DQKAIDKA LIANIKKVDM LASYLASSFS LRKGDKPHLM KF

UniProtKB: 60S ribosomal protein L6

Macromolecule #12: 60S ribosomal protein l7-like protein

• Macromolecule: Name: 60S ribosomal protein l7-like protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 28.821816 KDa

Sequence

String:

MSSTTVPTQN DILVPETLLK KRKSQEKARA ERAAALEKRK QANKEKRQVI FKRAEKYVKE YREQEREKIR LARIAKQQGS FHIPAEAKL VFVIRIKGIN KIPPKPRKIL QLLRLRQINN GVFVKVTKAT AEMIKIVEPW VAYGYPNLKS VRELIYKRGY G KVNGQRIP LTDNAIIEEN LGKYGIICIE DLIHEIFTVG PNFKQAANFL WPFKLSNPNG GFRPRKFKHF IEGGDLGNRE EH INALIRA MN

UniProtKB: 60S ribosomal protein l7-like protein

Macromolecule #13: 60S ribosomal protein L8

• Macromolecule: Name: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 29.74449 KDa

Sequence

String:

MPPKSGKKVA PAPFPQGKAG AKKAPKNPLL EKRPRNFGIG QDIQPKRNLS RMVKWPEYIR LQRQKKILRM RLKVPPAIAQ FQYTLDKNL AAQAFKLLNK YRPETKQEKK ERLLREATAI KEGKKKEDVS KKPYTVKYGL NHVVGLIENK KASLVLIAND V DPIELVVF LPALCRKMGI PYAIIKGKAR LGTLVHKKTA AVVAITEVRS EDKNELAKLI SAVKEGYLEK VEDTRKRWGG GI MGFKAQK REEKRKKSLE TAIKV

UniProtKB: 60S ribosomal protein L8

Macromolecule #14: 60S ribosomal protein l9-like protein

• Macromolecule: Name: 60S ribosomal protein l9-like protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 21.582133 KDa

Sequence

String:

MRYIHSEETI PVPENLKVSI KSRLVTVEGP RGKLQKDLSH IAVNFSVVKK GVIGLEIHHG SRKDVAALRT VRTIINNMII GVTKGFKYK MRYVYAHFPI NVNVEKNAET GCYEVEIRNF IGEKIVRKVV MAPGVEVEIS KAQKDELILS GNSLEAVSQS A ADIQQICK VRNKDIRKFL DGIYVSEKGN IVED

UniProtKB: 60S ribosomal protein l9-like protein

Macromolecule #15: 60S ribosomal protein L10-like protein

• Macromolecule: Name: 60S ribosomal protein L10-like protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 25.255373 KDa

Sequence

String:

MARRPARCYR YCKNKPYPKS RFNRGVPDPK IKIYDLGRKR ATVDDFPLCV HLVSNEYEQL SSEALEAARI CANKYLVKYA GKEGFHLRV RAHPYHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV DIGQILISVR TRDSNRAIAL EALRRCQYKF P GRQKIIVS KNWGFTPLRR EEYLEKKAAG RVRVDGAYVQ FLTNHGNLEE NMRRFPDAFK A

UniProtKB: 60S ribosomal protein L10-like protein

Macromolecule #16: Putative ribosomal protein

• Macromolecule: Name: Putative ribosomal protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 20.119275 KDa

Sequence

String:

MSSEKAQNPM RELRIQKLVL NISVGESGDR LTRAAKVLEQ LSGQTPVYSK ARYTVRQFGI RRNEKIAVHV TVRGPKAEEI LERGLKVKE YELRRRNFSE TGNFGFGISE HIDLGIKYDP SIGIYGMDFY CCMTRPGERV AKRRRCKSRI GASHRITREE T IRWFKQRF DGIVR

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #17: 60S ribosomal protein L13

• Macromolecule: Name: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 24.19118 KDa

Sequence

String:

MAIKHNQQIP NNHFRKHWQR RVRCHFDQPG KKVTRRLARR AKAAALAPRP VDKLRPIVRC PTVKYNRRTR LGRGFTLEEL KAAGIPRLL APTIGIAVDH RRKNLSEESL AANVQRLKDY KARLILFPRK SNKPKKADTP KDQQTAETTT SLRTSFGVEQ P LAPGFTEI SKSEIPAGIE GGAYRALRKA RSDARLVGVR EKRAKEKAEA EANKK

UniProtKB: 60S ribosomal protein L13

Macromolecule #18: 60S ribosomal protein L14-like protein

• Macromolecule: Name: 60S ribosomal protein L14-like protein / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 16.111901 KDa

Sequence

String:

MAEINVEATS WRRVEVGRVL KLENGSLAAI VEIIDHKRVL ADGPSSDPKL ATPRGIVPLS RALLTPIVIP KLPRGARTGA VKKAWEAYG VDAKWKETNW AKKQLQQERR QSLTDFDRFK VMRLKKQRRF EERKALAKIK AAA

UniProtKB: 60S ribosomal protein L14-like protein

Macromolecule #19: Ribosomal protein L15

• Macromolecule: Name: Ribosomal protein L15 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 24.307287 KDa

Sequence

String:

MGALKYLEEL SKKKQSDVVR FLLRVRCWEY RQLNVIHRAS RPSRPDKARR LGYKAKQGYV IYRVRVRRGG RKRPVPKGAT YGKPTNQGV NQLKYQRSLR ATAEERVGRR CSNLRVLNSY WVNQDSTYKY YEVILVDPNH KAIRRDPRIN WICNPVHKHR E CRGLTSTG KKSRGLNKGH RFNKTRAGRR KTWKRHNTLS LWRYR

UniProtKB: Ribosomal protein L15

Macromolecule #20: 60S ribosomal protein L16-like protein

• Macromolecule: Name: 60S ribosomal protein L16-like protein / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 22.888037 KDa

Sequence

String:

(ACE)SSFESVVVI DGKGHLLGRL ASIVAKQLLS GQKIVVVRCE ALNISGEFFR AKLKYHAYLR KMTRYNPTRG GPFHFR APS RIFYKAVRGM IPHKTARGAA ALERLKVFEG VPPPYDKKKK MVVPQALRVL RLQPGRKYCT VGRLSHEVGW KYQDVVA RL EERRKAKGAA YYERKKLAAR QLSEAKKAAA AKVDPKVSEA LAAYGY

UniProtKB: 60S ribosomal protein L16-like protein

Macromolecule #21: 60S ribosomal protein l17-like protein

• Macromolecule: Name: 60S ribosomal protein l17-like protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 21.072207 KDa

Sequence

String:

MVRYAATEIA PTKSARARGS YLRVSFKNTR ETAQAINGWK LQRALTFLQN VIDKKEAVPM RRYAGSTGRT AQGKQWGVSR ARWPVKSAQ FLIGLLKNAE ANADAKGLDT GNLIIKHIQV NQAPKQRRRT YRAHGRINPY MSNPCHIELI LTEAEETVQK S EAVVRDVE SHLSSRQRGV RIRRALTAA

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #22: Ribosomal protein L18-like protein

• Macromolecule: Name: Ribosomal protein L18-like protein / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 24.195535 KDa

Sequence

String:

MDLVCAFIGP LFAPGARRLN LEEIAAESSS GIDLRWKHVR SSHRKAPKSD NVYLKLLVKL YRFLARRTEA PFNKVVLRRL FMSRINRPP VSLSRIARNL KNGNEKKTVV VVATVTDDNR LLQVPKMEVA ALRFTAKARA RIEAAGGRTL TLDQLALEKP T GANTLLLR GPKNAREAVK HFGFGPHKHK KPYVRSKGRK FERARGRRRS RGFKV

UniProtKB: Ribosomal protein L18-like protein

Macromolecule #23: Ribosomal protein L19

• Macromolecule: Name: Ribosomal protein L19 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 22.409201 KDa

Sequence

String:

MVNLRTQKRL AASVLGCGEG KVWLDPNEVS EISNANSRQS IRKLVADGLI IKKPVTMHSR SRARELNLAR RIGRHRGFGK RKGTADARM PQQVLWMRRQ RVLRRLLVKY RASGKIDKHL YHELYHLAKG NTFKHKRALV EHIHRAKAEK ARERQIKEEM D AKRARTKA ARERKQERQA AKRNALLGEE EESK

UniProtKB: Ribosomal protein L19

Macromolecule #24: 60S ribosomal protein L20

• Macromolecule: Name: 60S ribosomal protein L20 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 20.341893 KDa

Sequence

String:

MGRLQEYQVI GRHLPTEANP NPALYRMRIF APNEVVAKSR FWYFLRGLKK VKKATGEIVS VNVISEKHPL KVKNFGIWLR YDSRSGTHN MYKEYRETSR VAAVEAMYAD MAARHRARFR SIHILKVVEL EKTEDVKRPY IKQLVAKNLS FPLPHRVPKI S TKKIFSAK RPSTFS

UniProtKB: 60S ribosomal protein L20

Macromolecule #25: 60S ribosomal protein l21-like protein

• Macromolecule: Name: 60S ribosomal protein l21-like protein / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 18.159227 KDa

Sequence

String:

MGHAAGLRSG TRYAFSRGFR KHGQIPLSTY LRTYRVGDIV DIKVNGAVQK GMPHKFYHGK TGVVYNVTKS AVGVIVYKRV KHRYIEKRI NVRIEHVKPS RSREDFLRRV KENAELKKKA KAEGVPVQLK RQPAMPREAH TVSIADNKPV TLAPVAYETT I

UniProtKB: 60S ribosomal protein l21-like protein

Macromolecule #26: 60S ribosomal protein L22-like protein

• Macromolecule: Name: 60S ribosomal protein L22-like protein / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 14.302563 KDa

Sequence

String:

MAPVAKKSGA KGKGPKVTKK FIINASQPAS DKIFDVSAFE KFLNERIKVD GRVGNLGDVI KISQIGDGKV EIIAHNDLSG RYLKYLTKK FLKKMQLRDW LRVVATSKGV YELKFYNIVN DEAEEEED

UniProtKB: 60S ribosomal protein L22-like protein

Macromolecule #27: 60S ribosomal protein l23-like protein

• Macromolecule: Name: 60S ribosomal protein l23-like protein / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 14.674428 KDa

Sequence

String:

MAKQKRGAPG GKLKMTLGLP VGAIMNCADN SGARNLYIIA VKGAGARLNR LPAGGVGDMV MATVKKGKPE LRKKVHPAVI VRQAKPWKR FDGVFLYFED NAGVIVNPKG EMKGSAITGP VGKEAAELWP RIASNAGVVM

UniProtKB: 60S ribosomal protein l23-like protein

Macromolecule #28: 60S ribosomal protein L25-like protein

• Macromolecule: Name: 60S ribosomal protein L25-like protein / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 17.175334 KDa

Sequence

String:

MAPKDVKKGG ASKAAKGAQA KKAAQAALKG VHSHKKTKVR KSTTFHRPKT LVLSRAPKYP RKSIPHEPRL DEHKIIIHPL NTEGALKKI EEQNTLVFIV DVKANKAQIK QALKKLYDID TVKINTLIRP DGTKKAFARL TPDVDALDIA ATKLGLV

UniProtKB: 60S ribosomal protein L25-like protein

Macromolecule #29: 60S ribosomal protein L26-like protein

• Macromolecule: Name: 60S ribosomal protein L26-like protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 15.565322 KDa

Sequence

String:

MKVRPTVSSS RRKARKAHFS APSSVRRVIM SAPLSKELRE KYNVRSIPIR KGDEVQIVRG AHKDKEGKVT SVYRLKYVIH VERVTREKA TGQTVPIGIH PSNVVITKLH LDKDRENILA RIKAGREQVA KAKGKKTAA

UniProtKB: 60S ribosomal protein L26-like protein

Macromolecule #30: 60S ribosomal protein L27

• Macromolecule: Name: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 15.803652 KDa

Sequence

String:

MKFLKTSRVC LVTRGRYAGK KVVIIQPVDQ GTKSHPYGHA LVAGIERYPS KITRRMSKAR IEKRSKIKPF IKVINYNHLM PTRYTLELE GLKAVINADT FKEPSQREEA KKTVKKVLED RYNSGKNRWF FTPLRF

UniProtKB: 60S ribosomal protein L27

Macromolecule #31: 60S ribosomal protein L28-like protein

• Macromolecule: Name: 60S ribosomal protein L28-like protein / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 16.848539 KDa

Sequence

String:

MPTRFSKTRK HRGHVSAGKG RVGKHRKHPG GRGMAGGQHH HRTNLDKYHP GYFGKVGMRH FHLLRNHYWR PSINIDKLWS LVPSDVREQ YLSGQKKDTA PVIDLLSHGY AKLLGKGRLP EIPVVVRARY VSAEAERKVK EAGGVVELVA

Macromolecule #32: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 7.529659 KDa

Sequence

String:

MAKSKNSSQH NQSRKAHRNG IKKPKTQRYP SLKGTDPKFR RNHRHALHGT AKALKEFKEG KRETA

UniProtKB: 60S ribosomal protein L29

Macromolecule #33: 60S ribosomal protein l30-like protein

• Macromolecule: Name: 60S ribosomal protein l30-like protein / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 11.602616 KDa

Sequence

String:

MAPKKSKSEA QSIGAKLALV IKSGKVVLGY RSTLKALRSG KAKLILIAAN TPPLRKSELE YYSMMSKTPV HHYSGTNIEL GTACGKLFR CSTMAILDAG DSDILADQQ

UniProtKB: 60S ribosomal protein l30-like protein

Macromolecule #34: Putative 60S ribosomal protein

• Macromolecule: Name: Putative 60S ribosomal protein / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 13.872177 KDa

Sequence

String:

MSSTQKKQRS AIADVVAREY TIHLHKRLHG VTFKKRAPRA IKEIKKFAQK AMGTSDVRLD PQLNKKVWEQ GIKGVPFRIR VRISRRRND EEGAKEKLYS YVQAVNVKNP KGLLTSVVEE E

UniProtKB: Putative 60S ribosomal protein

Macromolecule #35: 60S ribosomal protein L32-like protein

• Macromolecule: Name: 60S ribosomal protein L32-like protein / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 15.095985 KDa

Sequence

String:

MVAAKKHVPI VKKRTKRFMR HQSDRFKCLD SAWRKPKGID NRVRRRFKGN LAMPSIGYGS NKKTKHMMPS GHKAFLVHNV KDVELLLMH NRTYAAEIAH NVSSRKRIDI ITRAKQLGVK VTNAKAKVTT EV

UniProtKB: 60S ribosomal protein L32-like protein

Macromolecule #36: 60S ribosomal protein l33-like protein

• Macromolecule: Name: 60S ribosomal protein l33-like protein / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 12.330337 KDa

Sequence

String:

MPSEAGHRLY VKGRHLSYQR GRRNTHPKTS LIKIEGVDDT AAANFYLGKR VAYVYRAQKE VRGTKIRVIW GKITRPHGNS GVVRAKFTH PLPARSFGAS VRIMLYPSSI

UniProtKB: 60S ribosomal protein l33-like protein

Macromolecule #37: Ribosomal protein l34-like protein

• Macromolecule: Name: Ribosomal protein l34-like protein / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 13.492993 KDa

Sequence

String:

MAPTRVTYRR RNPYNTTSNR TRVIKTPGGQ LRVLHIKKRG TAPKCGDCGI KLPGIPALRP REYATISKPK KTVQRAYGGS RCGNCVRDR IIRAFLIEEQ KIVKKVLKEQ SQAEKKASKK

UniProtKB: Ribosomal protein l34-like protein

Macromolecule #38: Dolichyl-diphosphooligosaccharide--protein glycotransferase

• Macromolecule: Name: Dolichyl-diphosphooligosaccharide--protein glycotransferase; type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO; EC number: dolichyl-diphosphooligosaccharide-protein glycotransferase
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 14.643441 KDa

Sequence

String:

MSSNGKVKAG QLWSKNKEEL TKILGELKTE LSQLRIQKIS SSGAKLNKIH DLRKSIARVL TVINAKQRAQ LRLFYKNKKY LPLDLRPKL TRALRRRLSK EDASRVLEKT KKRLTHFPQR KYAVKAA

UniProtKB: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3

Macromolecule #39: 60S ribosomal protein L36

• Macromolecule: Name: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 12.784053 KDa

Sequence

String:

MSEDATPKAP VERTGLIRGL NKGHKTTRRV LKERPSRRKG AQSKRTQFVR SLIREVAGLA PYERRVIELL RNGKDKRARK FSKKKLGTF GRAKRKCEEL QRIIAESRRA H

UniProtKB: 60S ribosomal protein L36

Macromolecule #40: Ribosomal protein L37

• Macromolecule: Name: Ribosomal protein L37 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 10.660455 KDa

Sequence

String:

MTKGTSSFGK RHNKTHGLCR RCGRRSLHNQ KKVCASCGYP AAKTRKYNWS EKAKRRKVTG TGRMRYLSTV PRRFKNGFRT GVPKGARGP AVSTSS

UniProtKB: Ribosomal protein L37

Macromolecule #41: 60S ribosomal protein L38-like protein

• Macromolecule: Name: 60S ribosomal protein L38-like protein / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 9.537339 KDa

Sequence

String:

MPQEVSDIKK FIEICRRKDA SSARIKKNPK TQQIKFKVRC QRFLYTLVLK DSDKAEKLKQ SLPPNLQIKD VPKRNKRKSS A

Macromolecule #42: Ribosomal protein eL39

• Macromolecule: Name: Ribosomal protein eL39 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 6.297535 KDa

Sequence

String:

MPSHKTFRIK QKLAKAQKQN RPIPQWIRLR TGNTIRYNAK RRHWRKTRLG L

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #43: Putative ribosomal protein

• Macromolecule: Name: Putative ribosomal protein / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 14.609222 KDa

Sequence

String:

MQIFVKTLTG KTITLEVESS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLKALASKF NCDKMICRKC YARLPPRATN CRKRKCGHSN QLRPKKKLK

Macromolecule #44: 60S ribosomal protein L41-A

• Macromolecule: Name: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 3.354243 KDa

Sequence

String:

MRAKWRKKRT RRLKRKRRKV RARSK

UniProtKB: Small ribosomal subunit protein eS32A

Macromolecule #45: 60S ribosomal protein L44-like protein

• Macromolecule: Name: 60S ribosomal protein L44-like protein / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 12.045451 KDa

Sequence

String:

MVNVPKTRKT FCKGKDCQKH TLHKVTQYKA GKASAFAQGK RRYDRKQSGY GGQTKPVFHK KAKTTKKIVL RLECSVCKTK KQLPLKRCK HFELGGDKKT KGAALVF

UniProtKB: 60S ribosomal protein L44-like protein

Macromolecule #46: 60S ribosomal protein L43-like protein

• Macromolecule: Name: 60S ribosomal protein L43-like protein / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 10.158977 KDa

Sequence

String:

MSKRTKKVGI SGKYGTRYGA SLRKLVKKQE VTQHARYTCT FCGKNSVRRT AVGIWSCKSC KKTMAGGAYT VSTPAAAAMR STLRRLREI TEA

UniProtKB: 60S ribosomal protein L43-like protein

Macromolecule #47: Putative 60S ribosomal protein

• Macromolecule: Name: Putative 60S ribosomal protein / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 15.960451 KDa

Sequence

String:

MSNVSADLIW EVSRNYNSFL VKQRTGTFSR DALNLTNQHS RKHAGFVNDK ALGIVPAEKG VKVIAKKVKA ANKPASSLYT VTYKSTARK AYKAIASQAA KHGYRADLRQ AAVARASAIL RSQRPVKPEP PKKLRGAAAR RAAAAGKQ

UniProtKB: Putative 60S ribosomal protein

Macromolecule #48: 26S rRNA

• Macromolecule: Name: 26S rRNA / type: rna / ID: 48 / Number of copies: 1
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 1.0787301249999999 MDa

Sequence

String:

AGGUUGACCU CGGAUCAGGU AGGAGGACCC GCUGAACUUA AGCAUAUCAA UAAGCGGAGG AAAAGAAACC AACAGGGAUU GCCCUAGUA ACGGCGAGUG AAGCGGCAAC AGCUCAAAUU UGAAAGCUGG CUUCGGCCCG CGUUGUAAUU UGGAGAGGAU G CUUUGGGC GAGGCUCCUU CUGAGUUCCC UGGAACGGGA CGCCACAGAG GGUGAGAGCC CCGUAUAGUU GGAAGCCAAG CC UGUGUAA AGCUCCUUCG ACGAGUCGAG UAGUUUGGGA AUGCUGCUCA AAAUGGGAGG UAAAUUUCUU CUAAAGCUAA AUA CCGGCC AGAGACCGAU AGCGCACAAG UAGAGUGAUC GAAAGAUGAA AAGCACUUUG AAAAGAGGGU UAAAUAGCAC GUGA AAUUG UUGAAAGGGA AGCGCUUGUG ACCAGACUUG CGCCCGGCGG AUCAUCCGGU GUUCUCACCG GUGCACUCCG CCGGG CUCA GGCCAGCAUC GGUUCUGGCG GGGGGAUAAA GGCCCAGGGA AUGUGGCUCC UCCGGGAGUG UUAUAGCCCU GGGUGU AAU ACCCUCGCCG GGACCGAGGA CCGCGCUCUG CAAGGAUGCU GGCGUAAUGG UCACCAGCGA CCCGUCUUGA AACACGG AC CAAGGAGUCA AGGUUUUGCG CGAGUGUUUG GGUGUAAAAC CCGCACGCGU AAUGAAAGUG AACGUAGGUG AGAGCUUC G GCGCAUCAUC GACCGAUCCU GAUGUAUUCG GAUGGAUUUG AGUAGGAGCG UUAAGCCUUG GACCCGAAAG AUGGUGAAC UAUGCUUGGA UAGGGUGAAG CCAGAGGAAA CUCUGGUGGA GGCUCGCAGC GGUUCUGACG UGCAAAUCGA UCGUCAAAUC UGAGCAUGG GGGCGAAAGA CUAAUCGAAC CAUCUAGUAG CUGGUUACCG CCGAAGUUUC CCUCAGGAUA GCAGUGUCGA C CUUCAGUU UUAUGAGGUA AAGCGAAUGA UUAGGGACUC GGGGGCGAUU UUUAGCCUUC AUCCAUUCUC AAACUUUAAA UA UGUAAGA AGCCCUUGUU ACUUAACUGA ACGUGGGCAU UCGAAUGUAU CGACACUAGU GGGCCAUUUU UGGUAAGCAG AAC UGGCGA UGCGGGAUGA ACCGAACGCG GGGUUAAGGU GCCGGAGUGG ACGCUCAUCA GACACCACAA AAGGCGUUAG UACA UCUUG ACAGCAGGAC GGUGGCCAUG GAAGUCGGAA UCCGCUAAGG ACUGUGUAAC AACUCACCUG CCGAAUGUAC UAGCC CUGA AAAUGGAUGG CGCUCAAGCG UCCCACCCAU ACCCCGCCCU CAGGGUAGAA ACGAUGCCCU GAGGAGUAGG CGGCCG UGG AGGUCAGUGA CGAAGCCUAG GGCGUGAGCC CGGGUCGAAC GGCCUCUAGU GCAGAUCUUG GUGGUAGUAG CAAAUAC UU CAAUGAGAAC UUGAAGGACC GAAGUGGGGA AAGGUUCCAU GUGAACAGCG GUUGGACAUG GGUUAGUCGA UCCUAAGC C AUAGGGAAGU UCCGUUUCAA AGGGGCACUC GUGCCCCGUG UGGCGAAAGG GAAGCCGGUU AAUAUUCCGG CACCUGGAU GUGGGUUUUG CGCGGCAACG CAACUGAACG CGGAGACGAC GGCGGGGGCC CCGGGCAGAG UUCUCUUUUC UUCUUAACGG UCUAUCACC CUGGAAACAG UUUGUCUGGA GAUAGGGUUU AAUGGCCGGA AGAGCCCGAC ACUUCUGUCG GGUCCGGUGC G CUCUCGAC GUCCCUUGAA AAUCCGCGGG AGGGAAUAAU UCUCACGCCA GGUCGUACUC AUAACCGCAG CAGGUCCCCA AG GUGAACA GCCUCUGGUU GAUAGAACAA UGUAGAUAAG GGAAGUCGGC AAAAUAGAUC CGUAACUUCG GGAAAAGGAU UGG CUCUAA GGGUUGGGCA CGUUGGGCUU UGGGCGGACG CCCUGGGAGC AGAGGGCCUC UAGCCGGGCA ACCGGCCGGC GGCC CUCAG CACCCGGGGU UGAAGCCCUU AGCAGGCUUC GGCCGUCCGG CGUGCGGUUA ACAACCAACU UAGAACUGGU ACGGA CAGG GGGAAUCUGA CUGUCUAAUU AAAACAUAGC AUUGCGAUGG CCAGAAAGUG GUGUUGACGC AAUGUGAUUU CUGCCC AGU GCUCUGAAUG UCAAAGUGAA GAAAUUCAAC CAAGCGCGGG UAAACGGCGG GAGUAACUAU GACUCUCUUA AGGUAGC CA AAUGCCUCGU CAUCUAAUUA GUGACGCGCA UGAAUGGAUU AACGAGAUUC CCACUGUCCC UAUCUACUAU CUAGCGAA A CCACAGCCAA GGGAACGGGC UUGGCAAAAU CAGCGGGGAA AGAAGACCCU GUUGAGCUUG ACUCUAGUUU GACAUUGUG AAAAGACAUA GGAGGUGUAG AAUAGGUGGG AGCUUCGGCG CCAGUGAAAU ACCACUACUC CUAUUGUUUU UUUACUUAUU CAAUGAAGC GGGGCUGGAC UUGCGUCCAA CUUCUGGAGU UAAGGUCCUU CGCGGGCCGA CCCGGGUUGA AGACAUUGUC A GGUGGGGA GUUUGGCUG(OMG) GGCGGCACAU CUGUUAAACC AUAACGCAGG UGUCCUAAGG GGGGCUCAUG GAGAACAG A AAUCUCCAGU AGAACAAAAG GGUAAAAGUC CCCUUGAUUU UGAUUUUCAG UGUGAAUACA AACCAUGAAA GUGUGGCCU AUCGAUCCUU UAGUCCCUCG AAAUUUGAGG CUAGAGGUGC CAGAAAAGUU ACCACAGGGA UAACUGGCUU GUGGCGGCCA AGCGUUCAU AGCGACGUCG CUUUUUGAUC CUUCGAUGUC GGCUCUUCCU AUCAUACCGA AGCAGAAUUC GGUAAGCGUU G GAUUGUUC ACCCACUAAU AGGGAACGUG AGCUGGGUUU AGACCGUCGU GAGACAGGUU AGUUUUACCC UACUGAUGAA CU CGUCGCA AUGGUAAUUC AGCUUAGUAC GAGAGGAACC GCUGAUUCAG AUAAUUGGUU UUUGCGGUUG UCCGACCGGG CAG UGCCGC GAAGCUACCA UCUGCUGGAU AAUGGCUGAA CGCCUCUAAG UCAGAAUCCA UGCCAGAACG CGACGAUACU ACCC GCACG UUGUAGACGU AUAAGAAUAG GCUCCGGCCU CGUAUCCUAG CAGGCGAUUC CUCCGCCGGC CUCGAAGUGG CCGUC GGUA AUUCGCGUAU UGCAAUUUAG ACACGCGCGG GAUCAAAUCC UUUGCAGACG ACUUAGAUGU GCGAAAGGGU CCUGUA AGC AGUAGAGUAG CCUUGUUGUU ACGAUCUGCU GAGGGUAAGC CCUCCUUCGC CUAGAUUU

Macromolecule #49: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 49 / Number of copies: 1
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 50.147688 KDa

Sequence

String:

AAACUUUCAA CAACGGAUCU CUUGGUUCUG GCAUCGAUGA AGAACGCAGC GAAAUGCGAU AAGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC GCCGGUAUUC CGGCGGGCAU GCCUGUUCGA GCGUCAU

GENBANK: GENBANK: XR_002966750.1

Macromolecule #50: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 50 / Number of copies: 1
• Source (natural): Organism: Thermochaetoides thermophila DSM 1495 (fungus)
• Molecular weight: Theoretical: 38.596891 KDa

Sequence

String:

ACAUACGACC AUACCUAGUG GAAAACUCGG GAUCCCGUCC GCUCUCCCAU AGAUAAGCCA CUAAGGGCGG GGUUAGUACU CAGGUCGGU GACGACUGGG GAAUCCCCCG UGUUGUAUGU U

GENBANK: GENBANK: 7OLC_3

Macromolecule #51: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 51 / Number of copies: 379 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.6 mg/mL

Buffer

pH: 8
Component:

Concentration	Formula	Name
20.0 mM	HEPES	4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
100.0 mM	KOAc	Potassium acetate
10.0 mM	Mg(OAc)2	Magnesium acetate
0.25 %	Digitonin	Digitonin

• Grid: Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 3 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 90 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV / Details: waiting time: 15 s blot force: 0.

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 105000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 2108591
• CTF correction: Software - Name: cryoSPARC (ver. 4.4.0) / Software - details: patch-CTF / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: INSILICO MODEL
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.4.0); Software - details: non-uniform refinement with CTF refinements; Details: non-uniform refinement with CTF refinements / Number images used: 119533
• Initial angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: cryoSPARC (ver. 4.4.0) / Software - details: ab-initio reconstruction
• Final angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: cryoSPARC (ver. 4.4.0) / Software - details: non-uniform refinement
• Final 3D classification: Number classes: 10 / Avg.num./class: 40000 / Software - Name: cryoSPARC (ver. 4.4.0) / Software - details: 3D classification without alignment

Atomic model buiding 1

Initial model

Chain	Details	PDB ID
source_name: AlphaFold, initial_model_type: in silico model	complex of CCDC47 and SND3
source_name: AlphaFold, initial_model_type: in silico model	complex of SEC61 translocon
source_name: AlphaFold, initial_model_type: in silico model	complex of SEC61 translocon and TRAP alpha subunit
source_name: PDB, initial_model_type: experimental model	ribosome	7olc

• Refinement: Protocol: RIGID BODY FIT

Output model

PDB-9i78:
Cryo-EM structure of Chaetomium thermophilum ribosome-bound SND3 translocon