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- EMDB-51788: Cryo EM structure of RC-dLH complex model II from Gemmatimonas gr... -

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Basic information

Entry
Database: EMDB / ID: EMD-51788
TitleCryo EM structure of RC-dLH complex model II from Gemmatimonas groenlandica
Map data
Sample
  • Complex: RC-dLH complex model II from Gemmatimonas groenlandica
    • Protein or peptide: x 9 types
  • Ligand: x 11 types
Keywordsphotosynthetic bacteria / photosynthesis / reaction cennter light harvesting complex / RC-LH1 / RC-dLH / cryo-EM / Gemmatimonas groenlandica
Function / homology
Function and homology information


organelle inner membrane / plasma membrane light-harvesting complex / bacteriochlorophyll binding / photosynthetic electron transport in photosystem II / photosynthesis, light reaction / endomembrane system / electron transfer activity / iron ion binding / heme binding / metal ion binding ...organelle inner membrane / plasma membrane light-harvesting complex / bacteriochlorophyll binding / photosynthetic electron transport in photosystem II / photosynthesis, light reaction / endomembrane system / electron transfer activity / iron ion binding / heme binding / metal ion binding / membrane / plasma membrane
Similarity search - Function
Photosynthetic reaction centre, cytochrome c subunit / Multihaem cytochrome, PRC subunit superfamily / Photosynthetic reaction centre cytochrome C subunit / Antenna complex, beta subunit, conserved site / Antenna complexes beta subunits signature. / Antenna complex, alpha subunit / Antenna complex, alpha subunit conserved site / Antenna complexes alpha subunits signature. / Antenna complex, alpha/beta subunit / Light-harvesting protein B beta chain ...Photosynthetic reaction centre, cytochrome c subunit / Multihaem cytochrome, PRC subunit superfamily / Photosynthetic reaction centre cytochrome C subunit / Antenna complex, beta subunit, conserved site / Antenna complexes beta subunits signature. / Antenna complex, alpha subunit / Antenna complex, alpha subunit conserved site / Antenna complexes alpha subunits signature. / Antenna complex, alpha/beta subunit / Light-harvesting protein B beta chain / Antenna complex, beta domain superfamily / Antenna complex alpha/beta subunit / Light-harvesting complex / Photosynthetic reaction centre, M subunit / Photosynthetic reaction centre, L subunit / Multiheme cytochrome superfamily / : / Photosynthetic reaction centre, L/M / Photosystem II protein D1/D2 superfamily / Photosynthetic reaction centre protein / Photosynthetic reaction center proteins signature. / Prokaryotic membrane lipoprotein lipid attachment site profile.
Similarity search - Domain/homology
Reaction center protein M chain / Light-harvesting protein / Reaction center protein L chain / Uncharacterized protein / Light-harvesting protein / Light-harvesting protein / Photosynthetic reaction center cytochrome c subunit
Similarity search - Component
Biological speciesGemmatimonas groenlandica (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.3 Å
AuthorsGardiner AT / Jing Y / Bina D / Mujakic I / Gardian Z / Kaftan D / Joosten M / Jakobi A / Castro-Hartmann P / Qian P / Koblizek M
Funding support Czech Republic, 1 items
OrganizationGrant numberCountry
Czech Academy of Sciences19-28778X Czech Republic
CitationJournal: To Be Published
Title: Cryo EM structure of RC-dLH complex model II from Gemmatimonas groenlandica
Authors: Gardiner AT / Jing Y / Bina D / Mujakic I / Gardian Z / Kaftan D / Joosten M / Jakobi A / Castro-Hartmann P / Qian P / Koblizek M
History
DepositionOct 10, 2024-
Header (metadata) releaseDec 3, 2025-
Map releaseDec 3, 2025-
UpdateDec 3, 2025-
Current statusDec 3, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_51788.png

Downloads & links

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Map

FileDownload / File: emd_51788.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.73 Å/pix.
x 600 pix.
= 439.2 Å		0.73 Å/pix.
x 600 pix.
= 439.2 Å		0.73 Å/pix.
x 600 pix.
= 439.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.732 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0687
Minimum - Maximum-0.22641595 - 0.54656935
Average (Standard dev.)0.0005516693 (±0.012068127)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions600600600
Spacing600600600
CellA=B=C: 439.19998 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_51788_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_51788_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : RC-dLH complex model II from Gemmatimonas groenlandica

EntireName: RC-dLH complex model II from Gemmatimonas groenlandica
Components
  • Complex: RC-dLH complex model II from Gemmatimonas groenlandica
    • Protein or peptide: Light-harvesting protein
    • Protein or peptide: Light-harvesting protein
    • Protein or peptide: Light-harvesting protein
    • Protein or peptide: Photosynthetic reaction center cytochrome c subunit
    • Protein or peptide: reaction centre Ht su unit
    • Protein or peptide: reaction centre Hc sub unit
    • Protein or peptide: Reaction center protein L chain
    • Protein or peptide: Reaction center protein M chain
    • Protein or peptide: reaction centre S sub unit
  • Ligand: BACTERIOCHLOROPHYLL A
  • Ligand: BACTERIOPHEOPHYTIN A
  • Ligand: DODECYL-BETA-D-MALTOSIDE
  • Ligand: MENAQUINONE 8
  • Ligand: FE (III) ION
  • Ligand: (2R,5R,11R,14R)-5,8,11-trihydroxy-5,11-dioxido-17-oxo-2,14-bis(tetradecanoyloxy)-4,6,10,12,16-pentaoxa-5,11-diphosphatriacont-1-yl tetradecanoate
  • Ligand: SPIRILLOXANTHIN
  • Ligand: 1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE
  • Ligand: HEME C
  • Ligand: (2~{E},4~{E},6~{E},10~{E},12~{E},14~{E},16~{E},18~{E},20~{E},22~{Z},24~{E},26~{E},28~{E})-23-methanoyl-31-methoxy-2,6,10,14,19,27,31-heptamethyl-dotriaconta-2,4,6,10,12,14,16,18,20,22,24,26,28-tridecaenoic acid
  • Ligand: water

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Supramolecule #1: RC-dLH complex model II from Gemmatimonas groenlandica

SupramoleculeName: RC-dLH complex model II from Gemmatimonas groenlandica
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #7, #9, #8, #6, #4-#5, #2-#3, #1 / Details: a monomer complex
Source (natural)Organism: Gemmatimonas groenlandica (bacteria)
Molecular weightTheoretical: 650 KDa

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Macromolecule #1: reaction centre S sub unit

MacromoleculeName: reaction centre S sub unit / type: protein_or_peptide / ID: 1 / Details: native protein / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Gemmatimonas groenlandica (bacteria)
Molecular weightTheoretical: 21.641564 KDa
SequenceString: MMLRQDRPRV LSRVSTVVGI GALVAVAMAA RSQGGQQATP PSAPQPPVAA PSAAPAATDS TMQDSTQRAD TTAKADSMLA MPDSMMMQH TAAAPAPQAS AMWPVDPVTG QTIINGEPVV GRVFIMQKTD GTVKLGKWQA QYDGEPTAPE AANVGSSYTV P APEHTRRM ...String:
MMLRQDRPRV LSRVSTVVGI GALVAVAMAA RSQGGQQATP PSAPQPPVAA PSAAPAATDS TMQDSTQRAD TTAKADSMLA MPDSMMMQH TAAAPAPQAS AMWPVDPVTG QTIINGEPVV GRVFIMQKTD GTVKLGKWQA QYDGEPTAPE AANVGSSYTV P APEHTRRM RGIMIQSTLW SIDGKRSARE RRHYRPQTTG AALGQQ

UniProtKB: Uncharacterized protein

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Macromolecule #2: Reaction center protein L chain

MacromoleculeName: Reaction center protein L chain / type: protein_or_peptide / ID: 2 / Details: native protein / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Gemmatimonas groenlandica (bacteria)
Molecular weightTheoretical: 30.54274 KDa
SequenceString: MAMLSFEKKY RVRGGTLIGG DLFDFWFGPF YVGFFGVTTI FFVTLGTLLC VWGAAMGPTW NLWQISIAPP DLKYGLGLAP LREGGLWQI ITLCALGAFG SWALRQAEIS RKLGMGMHIP WAYGGAVLAY ATLVVIRPML LGAWGHGFPY GIFSHLDWVS N VGYQYLHF ...String:
MAMLSFEKKY RVRGGTLIGG DLFDFWFGPF YVGFFGVTTI FFVTLGTLLC VWGAAMGPTW NLWQISIAPP DLKYGLGLAP LREGGLWQI ITLCALGAFG SWALRQAEIS RKLGMGMHIP WAYGGAVLAY ATLVVIRPML LGAWGHGFPY GIFSHLDWVS N VGYQYLHF HYNPAHMIAV TFFFTNCLAL AMHGSLILSV TNPKKGTPVG TSETENVFFR DLLGYSIGAI GIHRLGLFLA VG AAVWSAI CIVISGPFWT KGWPEWWNWW LNLPIWR

UniProtKB: Reaction center protein L chain

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Macromolecule #3: Reaction center protein M chain

MacromoleculeName: Reaction center protein M chain / type: protein_or_peptide / ID: 3 / Details: native protein / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Gemmatimonas groenlandica (bacteria)
Molecular weightTheoretical: 43.396742 KDa
SequenceString: MLEYQNLFTR VQVRTVPEAG IEIDESTGTR YGTGTFSYLA GKFGDAQIGP IYLGWAGVLS LIFGFMAFEI IGLNMWASVG WDPVEFIRQ LPWLALEPPP PQYGLRVPPL AQGGWYLMAG FFLTISILLW WVRVYRRARA LNMGTHLPWA FASAIFLYST F FFQPLLVG ...String:
MLEYQNLFTR VQVRTVPEAG IEIDESTGTR YGTGTFSYLA GKFGDAQIGP IYLGWAGVLS LIFGFMAFEI IGLNMWASVG WDPVEFIRQ LPWLALEPPP PQYGLRVPPL AQGGWYLMAG FFLTISILLW WVRVYRRARA LNMGTHLPWA FASAIFLYST F FFQPLLVG SWSEMVPFGI FPHLDWTSAF SIRYGNLYYN PFHALSIAFL YGSAVLFAMH GATILAVARL GGEREIEQIT DR GTAAERS MLFWRWTMGF NATMESIHRW SWWFAVLTTF SGGIGILLTG TVVDNWYLWG VKHGLVAPYP AQNTLTEEQQ QLL RGRYQG TAPDSFPSYV APQPAMMLDS TAMMAPADSM KADSTKVDSA AAPSAPAAAA PPPAKPPAPS VGGKTP

UniProtKB: Reaction center protein M chain

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Macromolecule #4: reaction centre Ht su unit

MacromoleculeName: reaction centre Ht su unit / type: protein_or_peptide / ID: 4 / Details: a single helix / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Gemmatimonas groenlandica (bacteria)
Molecular weightTheoretical: 7.771843 KDa
SequenceString:
MQYIDGAQIA LYAFWLFFFG LIIYIRREDK REGYPLESPQ GPREGWPAMP EKKTYIHRPT SGEGTH

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Macromolecule #5: reaction centre Hc sub unit

MacromoleculeName: reaction centre Hc sub unit / type: protein_or_peptide / ID: 5 / Details: native protein / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Gemmatimonas groenlandica (bacteria)
Molecular weightTheoretical: 19.669318 KDa
SequenceString:
MSDIKAVPAD SYNGSALIPT GDPMIDGVGP SSWANRSDTP DMTFHNTAKI VPMRLDPTYS IAKGDPDPRG LPVVAADKQV AGTVIELWV NRAEPQVTYY EVQLTGSERR VMLPAGFVQW PNFGLWGNDK LLVKAITAAQ FANVPALKRD DQITLLEEDM V CAYYAGGH LYAMAERSEP II

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Macromolecule #6: Photosynthetic reaction center cytochrome c subunit

MacromoleculeName: Photosynthetic reaction center cytochrome c subunit / type: protein_or_peptide / ID: 6 / Details: native protein / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Gemmatimonas groenlandica (bacteria)
Molecular weightTheoretical: 40.302758 KDa
SequenceString: MSILRRSIGL VAPLALLSLG ACGDTATDSV QVGYRGTGME QNYDHGDLKK QFAQVKIPTP LPPAGESPPG PLPWQNVQVL NDISVGEFN RTMVAMSTWV AGTGNCAYCH NIANLAADTL PNGKPLYTKL VARRMLQMTR QINGQYSQHV KNTGVTCYTC H MGKPLPNG ...String:
MSILRRSIGL VAPLALLSLG ACGDTATDSV QVGYRGTGME QNYDHGDLKK QFAQVKIPTP LPPAGESPPG PLPWQNVQVL NDISVGEFN RTMVAMSTWV AGTGNCAYCH NIANLAADTL PNGKPLYTKL VARRMLQMTR QINGQYSQHV KNTGVTCYTC H MGKPLPNG LWFYSSQTDY LRHYLDRDGA RVVTRDVAPS NANRSSVKQT EWTYALMISQ SRSLGVNCTY CHNTRQFASW KE APPARVT AYHGILMLRD VNQNYLSPLQ PVYPSVRLGT QGDAPKAQCV TCHNGNYKPL YGAQMVKDYP ALWGRADWNG VPF QGLSPK ADTTSAGAAP AAPAAAAPVP AVKRSSARTV PAPTVIGGAV GSPNTPK

UniProtKB: Photosynthetic reaction center cytochrome c subunit

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Macromolecule #7: Light-harvesting protein

MacromoleculeName: Light-harvesting protein / type: protein_or_peptide / ID: 7 / Details: helix / Number of copies: 24 / Enantiomer: LEVO
Source (natural)Organism: Gemmatimonas groenlandica (bacteria)
Molecular weightTheoretical: 6.413583 KDa
SequenceString:
MHRIWQGMDP QIIMSGLGFF LAGLALIIHM WAYSITGWPK YKKAQYNAQT PPTAVR

UniProtKB: Light-harvesting protein

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Macromolecule #8: Light-harvesting protein

MacromoleculeName: Light-harvesting protein / type: protein_or_peptide / ID: 8 / Details: helix / Number of copies: 40 / Enantiomer: LEVO
Source (natural)Organism: Gemmatimonas groenlandica (bacteria)
Molecular weightTheoretical: 5.183985 KDa
SequenceString:
MMEKGGMTED EARRFHGYFV TGTLGYIIVA AVAHFLAWQW RPWF

UniProtKB: Light-harvesting protein

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Macromolecule #9: Light-harvesting protein

MacromoleculeName: Light-harvesting protein / type: protein_or_peptide / ID: 9 / Details: helix / Number of copies: 16 / Enantiomer: LEVO
Source (natural)Organism: Gemmatimonas groenlandica (bacteria)
Molecular weightTheoretical: 7.697123 KDa
SequenceString:
MHRIWLMFDP RRVMVAMVGF LAVLALVIHF ILLSSQRYSW IENGTLSAAQ APVGASAPAA AAEMSPLPPG R

UniProtKB: Light-harvesting protein

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Macromolecule #10: BACTERIOCHLOROPHYLL A

MacromoleculeName: BACTERIOCHLOROPHYLL A / type: ligand / ID: 10 / Number of copies: 84 / Formula: BCL
Molecular weightTheoretical: 911.504 Da
Chemical component information

ChemComp-BCL:
BACTERIOCHLOROPHYLL A

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Macromolecule #11: BACTERIOPHEOPHYTIN A

MacromoleculeName: BACTERIOPHEOPHYTIN A / type: ligand / ID: 11 / Number of copies: 2 / Formula: BPH
Molecular weightTheoretical: 889.215 Da
Chemical component information

ChemComp-BPH:
BACTERIOPHEOPHYTIN A

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Macromolecule #12: DODECYL-BETA-D-MALTOSIDE

MacromoleculeName: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 12 / Number of copies: 48 / Formula: LMT
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

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Macromolecule #13: MENAQUINONE 8

MacromoleculeName: MENAQUINONE 8 / type: ligand / ID: 13 / Number of copies: 3 / Formula: MQ8
Molecular weightTheoretical: 717.116 Da
Chemical component information

ChemComp-MQ8:
MENAQUINONE 8

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Macromolecule #14: FE (III) ION

MacromoleculeName: FE (III) ION / type: ligand / ID: 14 / Number of copies: 1 / Formula: FE
Molecular weightTheoretical: 55.845 Da

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Macromolecule #15: (2R,5R,11R,14R)-5,8,11-trihydroxy-5,11-dioxido-17-oxo-2,14-bis(te...

MacromoleculeName: (2R,5R,11R,14R)-5,8,11-trihydroxy-5,11-dioxido-17-oxo-2,14-bis(tetradecanoyloxy)-4,6,10,12,16-pentaoxa-5,11-diphosphatriacont-1-yl tetradecanoate
type: ligand / ID: 15 / Number of copies: 2 / Formula: CD4
Molecular weightTheoretical: 1.241633 KDa
Chemical component information

ChemComp-CD4:
(2R,5R,11R,14R)-5,8,11-trihydroxy-5,11-dioxido-17-oxo-2,14-bis(tetradecanoyloxy)-4,6,10,12,16-pentaoxa-5,11-diphosphatriacont-1-yl tetradecanoate

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Macromolecule #16: SPIRILLOXANTHIN

MacromoleculeName: SPIRILLOXANTHIN / type: ligand / ID: 16 / Number of copies: 1 / Formula: CRT
Molecular weightTheoretical: 596.925 Da
Chemical component information

ChemComp-CRT:
SPIRILLOXANTHIN

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Macromolecule #17: 1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE

MacromoleculeName: 1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE / type: ligand / ID: 17 / Number of copies: 17 / Formula: PEX
Molecular weightTheoretical: 522.632 Da
Chemical component information

ChemComp-PEX:
1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE

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Macromolecule #18: HEME C

MacromoleculeName: HEME C / type: ligand / ID: 18 / Number of copies: 4 / Formula: HEC
Molecular weightTheoretical: 618.503 Da
Chemical component information

ChemComp-HEC:
HEME C

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Macromolecule #19: (2~{E},4~{E},6~{E},10~{E},12~{E},14~{E},16~{E},18~{E},20~{E},22~{...

MacromoleculeName: (2~{E},4~{E},6~{E},10~{E},12~{E},14~{E},16~{E},18~{E},20~{E},22~{Z},24~{E},26~{E},28~{E})-23-methanoyl-31-methoxy-2,6,10,14,19,27,31-heptamethyl-dotriaconta-2,4,6,10,12,14,16,18,20,22,24,26,28-tridecaenoic acid
type: ligand / ID: 19 / Number of copies: 40 / Formula: V7N
Molecular weightTheoretical: 610.865 Da
Chemical component information

ChemComp-V7N:
(2~{E},4~{E},6~{E},10~{E},12~{E},14~{E},16~{E},18~{E},20~{E},22~{Z},24~{E},26~{E},28~{E})-23-methanoyl-31-methoxy-2,6,10,14,19,27,31-heptamethyl-dotriaconta-2,4,6,10,12,14,16,18,20,22,24,26,28-tridecaenoic acid

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Macromolecule #20: water

MacromoleculeName: water / type: ligand / ID: 20 / Number of copies: 38 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8 / Details: 10mM Tris-HCl buffer, 20 mM NaCl, pH 8.0
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK III / Details: blot time, 3 sec blot force, 3.
Detailsin buffer solution with detergent beta-DDM. ~4 mg/ml

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Electron microscopy

MicroscopeTFS KRIOS
TemperatureMin: 88.0 K / Max: 92.0 K
Specialist opticsEnergy filter - Name: TFS Selectris X / Energy filter - Slit width: 10 eV
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Number grids imaged: 1 / Number real images: 23012 / Average exposure time: 5.13 sec. / Average electron dose: 60.0 e/Å2 / Details: images were collected in AFIS model
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.4 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 165000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Detailswith energy filter slit of 10 eV
Particle selectionNumber selected: 935540
Details: cryosparc blob selection first, then model reference selection for final 3d reconstruction
CTF correctionSoftware - Name: CTFFIND (ver. 4.0) / Details: CTF collection was performed within cryosparc / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL / In silico model: cryosparc ab-init
Details: a subset of randomly picked-up particles were used for initial model generation.
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 2.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.2.1) / Details: performed in cryosparc / Number images used: 116858
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: cryoSPARC (ver. 4.2.1) / Details: cryosparc Ab-init
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: cryoSPARC (ver. 4.2.1)
Final 3D classificationNumber classes: 2 / Avg.num./class: 120000 / Software - Name: cryoSPARC (ver. 4.2.1)
Details: This complex has two confirmations. 2 classs 3D classification was used for the final separation of the two confirmation.
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelChain - Source name: AlphaFold / Chain - Initial model type: in silico model
DetailsInitial model was established with coot. The model was fitted into map using chimera. The refinement was performed y the use of Phenix.
RefinementSpace: REAL / Protocol: RIGID BODY FIT / Overall B value: 60 / Target criteria: map to model
Output model

PDB-9h22:
Cryo EM structure of RC-dLH complex model II from Gemmatimonas groenlandica

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