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基本情報
登録情報 | ![]() | |||||||||||||||||||||
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タイトル | 30S-TEC (TEC in expressome position) Inactive state 2 | |||||||||||||||||||||
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![]() | Transcription / translation / coupling / RIBOSOME | |||||||||||||||||||||
機能・相同性 | ![]() positive regulation of cytoplasmic translation / RNA polymerase complex / transcription elongation-coupled chromatin remodeling / submerged biofilm formation / cellular response to cell envelope stress / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / bacterial-type RNA polymerase core enzyme binding / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding ...positive regulation of cytoplasmic translation / RNA polymerase complex / transcription elongation-coupled chromatin remodeling / submerged biofilm formation / cellular response to cell envelope stress / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / bacterial-type RNA polymerase core enzyme binding / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / cytosolic DNA-directed RNA polymerase complex / misfolded RNA binding / Group I intron splicing / RNA folding / bacterial-type flagellum-dependent cell motility / nitrate assimilation / negative regulation of cytoplasmic translation / four-way junction DNA binding / regulation of mRNA stability / DNA-directed RNA polymerase complex / positive regulation of RNA splicing / transcription elongation factor complex / regulation of DNA-templated transcription elongation / DNA endonuclease activity / transcription antitermination / DNA-templated transcription initiation / cell motility / DNA-templated transcription termination / maintenance of translational fidelity / ribonucleoside binding / mRNA 5'-UTR binding / : / : / : / : / : / : / DNA-directed RNA polymerase / regulation of translation / ribosome biogenesis / ribosomal small subunit biogenesis / ribosomal small subunit assembly / response to heat / small ribosomal subunit / protein-containing complex assembly / cytosolic small ribosomal subunit / small ribosomal subunit rRNA binding / intracellular iron ion homeostasis / cytoplasmic translation / tRNA binding / single-stranded RNA binding / protein dimerization activity / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / response to antibiotic / mRNA binding / magnesium ion binding / DNA binding / RNA binding / zinc ion binding / membrane / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||||||||||||||
生物種 | ![]() ![]() | |||||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.1 Å | |||||||||||||||||||||
![]() | Rahil H / Weixlbaumer A / Webster MW | |||||||||||||||||||||
資金援助 | European Union, ![]() ![]() ![]()
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![]() | ![]() タイトル: Molecular basis of mRNA delivery to the bacterial ribosome. 著者: Michael W Webster / Adrien Chauvier / Huma Rahil / Andrea Graziadei / Kristine Charles / Nataliya Miropolskaya / Maria Takacs / Charlotte Saint-André / Juri Rappsilber / Nils G Walter / Albert Weixlbaumer / ![]() ![]() ![]() ![]() 要旨: Protein synthesis begins with the formation of a ribosome-messenger RNA (mRNA) complex. In bacteria, the small ribosomal subunit (30) is recruited to many mRNAs through base pairing with the Shine- ...Protein synthesis begins with the formation of a ribosome-messenger RNA (mRNA) complex. In bacteria, the small ribosomal subunit (30) is recruited to many mRNAs through base pairing with the Shine-Dalgarno (SD) sequence and RNA binding by ribosomal protein bS1. Translation can initiate on nascent mRNAs, and RNA polymerase (RNAP) can promote the recruitment of the pioneering 30. Here, we examined 30 recruitment to nascent mRNAs using cryo-electron microscopy, single-molecule fluorescence colocalization, and in-cell cross-linking mass spectrometry. We show that bS1 delivers the mRNA to the ribosome for SD duplex formation and 30 activation. Additionally, bS1 and RNAP stimulate translation initiation. Our work provides a mechanistic framework for how the SD duplex, ribosomal proteins, and RNAP cooperate in 30 recruitment to mRNAs and establish transcription-translation coupling. | |||||||||||||||||||||
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構造の表示
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-EMDBアーカイブ
マップデータ | ![]() | 740.4 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 50.5 KB 50.5 KB | 表示 表示 | ![]() |
画像 | ![]() | 90.7 KB | ||
Filedesc metadata | ![]() | 14.2 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 9guwMC ![]() 9gr1C ![]() 9gupC ![]() 9guqC ![]() 9gurC ![]() 9gusC ![]() 9gutC ![]() 9guuC ![]() 9guvC ![]() 9guxC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.84 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : 30S ribosomal subunit bound to TEC
+超分子 #1: 30S ribosomal subunit bound to TEC
+超分子 #2: TEC
+分子 #1: 16S ribosomal RNA
+分子 #22: mRNA
+分子 #2: 30S ribosomal protein S1
+分子 #3: 30S ribosomal protein S2
+分子 #4: Small ribosomal subunit protein uS3
+分子 #5: Small ribosomal subunit protein uS4
+分子 #6: 30S ribosomal protein S5
+分子 #7: Small ribosomal subunit protein bS6
+分子 #8: 30S ribosomal protein S7
+分子 #9: 30S ribosomal protein S8
+分子 #10: 30S ribosomal protein S9
+分子 #11: 30S ribosomal protein S10
+分子 #12: 30S ribosomal protein S11
+分子 #13: 30S ribosomal protein S12
+分子 #14: 30S ribosomal protein S13
+分子 #15: 30S ribosomal protein S14
+分子 #16: Small ribosomal subunit protein uS15
+分子 #17: 30S ribosomal protein S16
+分子 #18: 30S ribosomal protein S17
+分子 #19: 30S ribosomal protein S18
+分子 #20: 30S ribosomal protein S19
+分子 #21: 30S ribosomal protein S20
+分子 #23: Transcription termination/antitermination protein NusG
+分子 #24: DNA-directed RNA polymerase subunit alpha
+分子 #25: DNA-directed RNA polymerase subunit beta
+分子 #26: DNA-directed RNA polymerase subunit beta'
+分子 #27: DNA-directed RNA polymerase subunit omega
+分子 #28: Non-Template DNA strand
+分子 #29: Template DNA strand
+分子 #30: MAGNESIUM ION
+分子 #31: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | TFS KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 QUANTUM (4k x 4k) 平均電子線量: 49.95 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 0.8 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
初期モデル | モデルのタイプ: PDB ENTRY PDBモデル - PDB ID: |
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最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.1 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 20703 |
初期 角度割当 | タイプ: ANGULAR RECONSTITUTION |
最終 角度割当 | タイプ: ANGULAR RECONSTITUTION |