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- EMDB-51344: Cryo-EM map of Thromboxane A2 receptor-Gq Protein Complex bound t... -

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Basic information

Entry
Database: EMDB / ID: EMD-51344
TitleCryo-EM map of Thromboxane A2 receptor-Gq Protein Complex bound to I-BOP focused refined on the receptor
Map dataSharpened cryo-EM map of Thromboxane A2 receptor-Gq Protein Complex bound to I-BOP focused refined on the G proteins
Sample
  • Complex: Thromboxane A2 receptor-Gq Protein Complex
KeywordsSingle particle cryo-EM / GPCR / G-proteins / MEMBRANE PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.52 Å
AuthorsMatzov D / Krawinski P / Caffrey M / Shalev Benami M
Funding supportEuropean Union, 1 items
OrganizationGrant numberCountry
European Research Council (ERC)949364European Union
CitationJournal: To Be Published
Title: Cryo-EM structure of Thromboxane A2 receptor-miniGq Protein Complex bound to I-BOP
Authors: Krawinski P / Matzov D / Ryder A / Lal K / Karlov DS / Chalhoub G / McCormick PJ / Caffrey M / Tikhonova IG / Shalev Benami M
History
DepositionAug 14, 2024-
Header (metadata) releaseAug 27, 2025-
Map releaseAug 27, 2025-
UpdateAug 27, 2025-
Current statusAug 27, 2025Processing site: PDBe / Status: Released

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Structure visualization

Downloads & links

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Map

FileDownload / File: emd_51344.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationSharpened cryo-EM map of Thromboxane A2 receptor-Gq Protein Complex bound to I-BOP focused refined on the G proteins
Voxel sizeX=Y=Z: 0.8423 Å
Density
Contour LevelBy AUTHOR: 0.15
Minimum - Maximum-1.152663 - 1.570579
Average (Standard dev.)0.00022515627 (±0.019879406)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 303.228 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Thromboxane A2 receptor-Gq Protein Complex

EntireName: Thromboxane A2 receptor-Gq Protein Complex
Components
  • Complex: Thromboxane A2 receptor-Gq Protein Complex

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Supramolecule #1: Thromboxane A2 receptor-Gq Protein Complex

SupramoleculeName: Thromboxane A2 receptor-Gq Protein Complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 38.4 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 20.0 µm / Nominal defocus min: 8.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.52 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 242712
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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