EMDB-50927: Influenza A/H7N9 polymerase post-cleavage cap-snatching complex

Basic information

Entry

Database: EMDB / ID: EMD-50927

• Title: Influenza A/H7N9 polymerase post-cleavage cap-snatching complex
• Map data: H7N9 cap-snatching post-cleavage complex Consensus refinement, not sharpened

Sample

• Complex: Influenza A/H7N9 polymerase post-cleavage cap-snatching complex
  • Complex: Transcription elongation factors SPT4 and SPT5
    • Protein or peptide: x 3 types
  • Complex: RNA
    • RNA: x 3 types
  • Complex: Influenza virus RNA-dependent RNA polymerase
    • Protein or peptide: x 3 types
  • Complex: DNA
    • DNA: x 2 types
  • Complex: Human RNA polymerase II
    • Protein or peptide: x 12 types
• Ligand: x 4 types

• Keywords: Influenza virus / influenza A / cap-snatching / transcription / VIRUS

Function / homology

Function:

negative regulation of DNA-templated transcription, elongation / DSIF complex / regulation of transcription elongation by RNA polymerase II / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / mRNA Splicing - Major Pathway / positive regulation of DNA-templated transcription, elongation / Abortive elongation of HIV-1 transcript in the absence of Tat / transcription elongation-coupled chromatin remodeling / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / cap snatching / viral transcription / RNA polymerase II complex binding / negative regulation of transcription elongation by RNA polymerase II / symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / positive regulation of macroautophagy / RNA polymerase II transcribes snRNA genes / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / 7-methylguanosine mRNA capping / host cell mitochondrion / transcription by RNA polymerase III / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / transcription by RNA polymerase I / RNA polymerase I complex / transcription elongation by RNA polymerase I / RNA polymerase III complex / Formation of HIV elongation complex in the absence of HIV Tat / transcription-coupled nucleotide-excision repair / core promoter sequence-specific DNA binding / RNA polymerase II, core complex / tRNA transcription by RNA polymerase III / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / translation initiation factor binding / RNA Polymerase II Pre-transcription Events / DNA-directed RNA polymerase complex / transcription initiation at RNA polymerase II promoter / TP53 Regulates Transcription of DNA Repair Genes / DNA-templated transcription initiation / transcription elongation by RNA polymerase II / DNA-directed RNA polymerase activity / euchromatin / : / ribonucleoside binding / virion component / fibrillar center / DNA-directed RNA polymerase / endonuclease activity / nucleic acid binding / transcription by RNA polymerase II / Hydrolases; Acting on ester bonds / host cell cytoplasm / protein dimerization activity / nuclear speck / symbiont-mediated suppression of host gene expression / protein heterodimerization activity / viral translational frameshifting / RNA-directed RNA polymerase / viral RNA genome replication / RNA-directed RNA polymerase activity / nucleotide binding / mRNA binding / DNA-templated transcription / chromatin binding / regulation of DNA-templated transcription / nucleolus / host cell nucleus / enzyme binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / mitochondrion / DNA binding / RNA binding

Domain/homology:

Spt5, KOW domain repeat 6 / Transcription elongation factor SPT5, seventh KOW domain / Transcription elongation factor SPT5, sixth KOW domain / Transcription initiation Spt4 / Spt4 superfamily / Spt4/RpoE2 zinc finger / Spt4/RpoE2 zinc finger / Spt4/RpoE2 zinc finger / Transcription elongation factor SPT5, second KOW domain / Transcription elongation factor SPT5, fifth KOW domain / Transcription elongation factor SPT5, KOWx domain / Transcription elongation factor SPT5, KOW1 domain / Transcription elongation factor SPT5, fourth KOW domain / Transcription elongation factor Spt5, eukaryote / Spt5 transcription elongation factor, N-terminal / Spt5, KOW domain repeat 2 / Spt5, KOW domain repeat 3 / Spt5, KOW domain repeat 5 / Spt5 transcription elongation factor, acidic N-terminal / NGN domain, eukaryotic / Spt5, KOW domain repeat 1 / Spt5, KOW domain repeat 4 / Spt5 C-terminal domain / Spt5 C-terminal nonapeptide repeat binding Spt4 / NGN domain / Transcription elongation factor SPT5 / Early transcription elongation factor of RNA pol II, NGN section / Influenza RNA-dependent RNA polymerase subunit PB2 / PB2, C-terminal / Influenza RNA-dependent RNA polymerase subunit PB1 / Influenza RNA-dependent RNA polymerase subunit PB1 / : / : / Influenza RNA polymerase PB2 N-terminal region / Influenza RNA polymerase PB2 second domain / : / Influenza RNA polymerase PB2 middle domain / : / Influenza RNA polymerase PB2 C-terminal domain / : / Influenza RNA polymerase PB2 6th domain / : / Influenza RNA polymerase PB2 CAP binding domain / : / Influenza RNA polymerase PB2 helical domain / NusG, N-terminal / In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold. / Polymerase acidic protein / NusG, N-terminal domain superfamily / Influenza RNA-dependent RNA polymerase subunit PA / Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain / Influenza RNA-dependent RNA polymerase subunit PA / RNA-directed RNA polymerase, negative-strand RNA virus / RdRp of negative ssRNA viruses with segmented genomes catalytic domain profile. / DNA-directed RNA polymerase II subunit Rpb4-like / RNA polymerase Rpb1 C-terminal repeat / RNA polymerase II, heptapeptide repeat, eukaryotic / Eukaryotic RNA polymerase II heptapeptide repeat. / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb4/RPC9, core / DNA-directed RNA-polymerase II subunit / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / Pol II subunit B9, C-terminal zinc ribbon / RNA polymerase RBP11 / Rpb4/RPC9 superfamily / RNA polymerase subunit Rpb4/RPC9 / RNA polymerase Rpb4 / Zinc finger TFIIS-type signature. / HRDC-like superfamily / RNA polymerase Rpb7-like , N-terminal / RNA polymerase Rpb7-like, N-terminal domain superfamily / RNA polymerase subunit Rpb7-like / SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397 / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4 / DNA-directed RNA polymerase, M/15kDa subunit / RNA polymerases M/15 Kd subunit / RNA polymerase subunit 9 / DNA-directed RNA polymerase M, 15kDa subunit, conserved site / RNA polymerases M / 15 Kd subunits signature. / DNA-directed RNA polymerase subunit/transcription factor S / : / RNA polymerase, Rpb8 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerase Rpb8 / RNA polymerase subunit 8 / RNA polymerase, Rpb5, N-terminal / RNA polymerase Rpb5, N-terminal domain superfamily / RNA polymerase Rpb5, N-terminal domain / DNA-directed RNA polymerase, subunit RPB6 / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature.

Component:

DNA-directed RNA polymerase subunit beta / RNA polymerase II subunit D / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerase II subunit E / Polymerase acidic protein / RNA-directed RNA polymerase catalytic subunit / Transcription elongation factor SPT5 / DNA-directed RNA polymerase II subunit RPB9 / Transcription elongation factor SPT4 / Polymerase basic protein 2

• Biological species: Homo sapiens (human) / Influenza B virus / Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)) / synthetic construct (others) / Sus scrofa domesticus (domestic pig)
• Method: single particle reconstruction / cryo EM / Resolution: 3.1 Å
• Authors: Rotsch AH / Li D / Dienemann C / Cusack S / Cramer P

Funding support

Germany, European Union, 2 items

Organization	Grant number	Country
German Research Foundation (DFG)	SFB860	Germany
European Research Council (ERC)	882357	European Union

• Citation: Journal: To Be Published; Title: Mechanism of Co-Transcriptional Cap-Snatching by Influenza Polymerase; Authors: Rotsch AH / Li D / Dupont M / Krischuns T / Oberthuer C / Stelfox A / Lukarska M / Fianu I / Lidschreiber M / Naffakh N / Dienemann C / Cusack S / Cramer P

History

Deposition	Jul 7, 2024	-
Header (metadata) release	Jul 16, 2025	-
Map release	Jul 16, 2025	-
Update	Jul 16, 2025	-
Current status	Jul 16, 2025	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_50927.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: H7N9 cap-snatching post-cleavage complex Consensus refinement, not sharpened
• Voxel size: X=Y=Z: 1.05 Å

Density

Contour Level	By AUTHOR: 0.2
Minimum - Maximum	-0.75636834 - 2.0180683
Average (Standard dev.)	0.00037991416 (±0.046088353)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 448 448 448 Spacing 448 448 448
Cell	A=B=C: 470.39996 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_50927_msk_1.map

Additional map: H7N9 cap-snatching post-cleavage complex Consensus refinement, sharpened...

• File: emd_50927_additional_1.map
• Annotation: H7N9 cap-snatching post-cleavage complex Consensus refinement, sharpened

Additional map: H7N9 cap-snatching post-cleavage complex Interface focussed refinement, Mask...

• File: emd_50927_additional_10.map
• Annotation: H7N9 cap-snatching post-cleavage complex Interface focussed refinement, Mask

Additional map: H7N9 cap-snatching post-cleavage complex Interface focussed refinement, Not...

• File: emd_50927_additional_11.map
• Annotation: H7N9 cap-snatching post-cleavage complex Interface focussed refinement, Not sharpened

Additional map: H7N9 cap-snatching post-cleavage complex Stalk focussed refinement, Not...

• File: emd_50927_additional_12.map
• Annotation: H7N9 cap-snatching post-cleavage complex Stalk focussed refinement, Not sharpened

Additional map: H7N9 cap-snatching post-cleavage complex Pol II core focussed...

• File: emd_50927_additional_13.map
• Annotation: H7N9 cap-snatching post-cleavage complex Pol II core focussed refinement, Mask

Additional map: H7N9 cap-snatching post-cleavage complex Pol II core focussed...

• File: emd_50927_additional_14.map
• Annotation: H7N9 cap-snatching post-cleavage complex Pol II core focussed refinement, not sharpened

Additional map: H7N9 cap-snatching post-cleavage complex Interface focussed refinement, Half...

• File: emd_50927_additional_15.map
• Annotation: H7N9 cap-snatching post-cleavage complex Interface focussed refinement, Half map B

Additional map: H7N9 cap-snatching post-cleavage complex FluPol focussed refinement, mask...

• File: emd_50927_additional_16.map
• Annotation: H7N9 cap-snatching post-cleavage complex FluPol focussed refinement, mask

Additional map: H7N9 cap-snatching post-cleavage complex Pol II core focussed...

• File: emd_50927_additional_17.map
• Annotation: H7N9 cap-snatching post-cleavage complex Pol II core focussed refinement, Half map A

Additional map: H7N9 cap-snatching post-cleavage complex Pol II core focussed...

• File: emd_50927_additional_18.map
• Annotation: H7N9 cap-snatching post-cleavage complex Pol II core focussed refinement, Sharpened

Additional map: H7N9 cap-snatching post-cleavage complex FluPol focussed refinement, sharpened...

• File: emd_50927_additional_19.map
• Annotation: H7N9 cap-snatching post-cleavage complex FluPol focussed refinement, sharpened

Additional map: H7N9 cap-snatching post-cleavage complex FluPol focussed refinement, Half...

• File: emd_50927_additional_2.map
• Annotation: H7N9 cap-snatching post-cleavage complex FluPol focussed refinement, Half map B

Additional map: H7N9 cap-snatching post-cleavage complex Stalk focussed refinement, Not...

• File: emd_50927_additional_20.map
• Annotation: H7N9 cap-snatching post-cleavage complex Stalk focussed refinement, Not sharpened

Additional map: H7N9 cap-snatching post-cleavage complex Stalk focussed refinement, Mask...

• File: emd_50927_additional_21.map
• Annotation: H7N9 cap-snatching post-cleavage complex Stalk focussed refinement, Mask

Additional map: H7N9 cap-snatching post-cleavage complex FluPol focussed refinement, Half...

• File: emd_50927_additional_3.map
• Annotation: H7N9 cap-snatching post-cleavage complex FluPol focussed refinement, Half map A

Additional map: H7N9 cap-snatching post-cleavage complex Pol II core focussed...

• File: emd_50927_additional_4.map
• Annotation: H7N9 cap-snatching post-cleavage complex Pol II core focussed refinement, Half map B

Additional map: H7N9 cap-snatching post-cleavage complex Interface focussed refinement, Sharpened...

• File: emd_50927_additional_5.map
• Annotation: H7N9 cap-snatching post-cleavage complex Interface focussed refinement, Sharpened

Additional map: H7N9 cap-snatching post-cleavage complex Stalk focussed refinement, Half...

• File: emd_50927_additional_6.map
• Annotation: H7N9 cap-snatching post-cleavage complex Stalk focussed refinement, Half map B

Additional map: H7N9 cap-snatching post-cleavage complex FluPol focussed refinement, not...

• File: emd_50927_additional_7.map
• Annotation: H7N9 cap-snatching post-cleavage complex FluPol focussed refinement, not sharpened

Additional map: H7N9 cap-snatching post-cleavage complex Stalk focussed refinement, Half...

• File: emd_50927_additional_8.map
• Annotation: H7N9 cap-snatching post-cleavage complex Stalk focussed refinement, Half map A

Additional map: H7N9 cap-snatching post-cleavage complex Interface focussed refinement, Half...

• File: emd_50927_additional_9.map
• Annotation: H7N9 cap-snatching post-cleavage complex Interface focussed refinement, Half map A

Half map: H7N9 cap-snatching post-cleavage complex Consensus refinement, Half map...

• File: emd_50927_half_map_1.map
• Annotation: H7N9 cap-snatching post-cleavage complex Consensus refinement, Half map A

Half map: H7N9 cap-snatching post-cleavage complex Consensus refinement, Half map...

• File: emd_50927_half_map_2.map
• Annotation: H7N9 cap-snatching post-cleavage complex Consensus refinement, Half map B

Sample components

Entire : Influenza A/H7N9 polymerase post-cleavage cap-snatching complex

• Entire: Name: Influenza A/H7N9 polymerase post-cleavage cap-snatching complex

Components

• Complex: Influenza A/H7N9 polymerase post-cleavage cap-snatching complex
  • Complex: Transcription elongation factors SPT4 and SPT5
    • Protein or peptide: Transcription elongation factor SPT5
    • Protein or peptide: Transcription elongation factor SPT4
    • Protein or peptide: likely linker between KOWx4 and KOW5 domains of SPT5
  • Complex: RNA
    • RNA: 5' cap(1) RNA
    • RNA: 3' vRNA
    • RNA: 5' vRNA
  • Complex: Influenza virus RNA-dependent RNA polymerase
    • Protein or peptide: Polymerase acidic protein
    • Protein or peptide: RNA-directed RNA polymerase catalytic subunit
    • Protein or peptide: Polymerase basic protein 2
  • Complex: DNA
    • DNA: nontemplate DNA (43-mer)
    • DNA: template DNA (43-mer)
  • Complex: Human RNA polymerase II
    • Protein or peptide: DNA-directed RNA polymerase subunit
    • Protein or peptide: DNA-directed RNA polymerase subunit beta
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB3
    • Protein or peptide: DNA-directed RNA polymerase II subunit E
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC2
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB9
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC5
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB11-a
    • Protein or peptide: RNA polymerase II, I and III subunit K
    • Protein or peptide: RNA polymerase II subunit D
    • Protein or peptide: DNA-directed RNA polymerase subunit
• Ligand: ZINC ION
• Ligand: MAGNESIUM ION
• Ligand: 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-GUANOSINE
• Ligand: PHOSPHATE ION

Supramolecule #1: Influenza A/H7N9 polymerase post-cleavage cap-snatching complex

• Supramolecule: Name: Influenza A/H7N9 polymerase post-cleavage cap-snatching complex; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#23

Supramolecule #2: Transcription elongation factors SPT4 and SPT5

• Supramolecule: Name: Transcription elongation factors SPT4 and SPT5 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#2, #10
• Source (natural): Organism: Homo sapiens (human)

Supramolecule #3: RNA

• Supramolecule: Name: RNA / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #4, #8-#9
• Source (natural): Organism: Influenza B virus

Supramolecule #4: Influenza virus RNA-dependent RNA polymerase

• Supramolecule: Name: Influenza virus RNA-dependent RNA polymerase / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #5-#7
• Source (natural): Organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))

Supramolecule #5: DNA

• Supramolecule: Name: DNA / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #20-#21
• Source (natural): Organism: synthetic construct (others)

Supramolecule #6: Human RNA polymerase II

• Supramolecule: Name: Human RNA polymerase II / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #3, #11-#19, #22-#23
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)

Macromolecule #1: Transcription elongation factor SPT5

• Macromolecule: Name: Transcription elongation factor SPT5 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 121.145477 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MSDSEDSNFS EEEDSERSSD GEEAEVDEER RSAAGSEKEE EPEDEEEEEE EEEYDEEEEE EDDDRPPKKP RHGGFILDEA DVDDEYEDE DQWEDGAEDI LEKEEIEASN IDNVVLDEDR SGARRLQNLW RDQREEELGE YYMKKYAKSS VGETVYGGSD E LSDDITQQ QLLPGVKDPN LWTVKCKIGE ERATAISLMR KFIAYQFTDT PLQIKSVVAP EHVKGYIYVE AYKQTHVKQA IE GVGNLRL GYWNQQMVPI KEMTDVLKVV KEVANLKPKS WVRLKRGIYK DDIAQVDYVE PSQNTISLKM IPRIDYDRIK ARM SLKDWF AKRKKFKRPP QRLFDAEKIR SLGGDVASDG DFLIFEGNRY SRKGFLFKSF AMSAVITEGV KPTLSELEKF EDQP EGIDL EVVTESTGKE REHNFQPGDN VEVCEGELIN LQGKILSVDG NKITIMPKHE DLKDMLEFPA QELRKYFKMG DHVKV IAGR FEGDTGLIVR VEENFVILFS DLTMHELKVL PRDLQLCSET ASGVDVGGQH EWGELVQLDP QTVGVIVRLE RETFQV LNM YGKVVTVRHQ AVTRKKDNRF AVALDSEQNN IHVKDIVKVI DGPHSGREGE IRHLFRSFAF LHCKKLVENG GMFVCKT RH LVLAGGSKPR DVTNFTVGGF APMSPRISSP MHPSAGGQRG GFGSPGGGSG GMSRGRGRRD NELIGQTVRI SQGPYKGY I GVVKDATEST ARVELHSTCQ TISVDRQRLT TVGSRRPGGM TSTYGRTPMY GSQTPMYGSG SRTPMYGSQT PLQDGSRTP HYGSQTPLHD GSRTPAQSGA WDPNNPNTPS RAEEEYEYAF DDEPTPSPQA YGGTPNPQTP GYPDPSSPQV NPQYNPQTPG TPAMYNTDQ FSPYAAPSPQ GSYQPSPSPQ SYHQVAPSPA GYQNTHSPAS YHPTPSPMAY QASPSPSPVG YSPMTPGAPS P GGYNPHTP GSGIEQNSSD WVTTDIQVKV RDTYLDTQVV GQTGVIRSVT GGMCSVYLKD SEKVVSISSE HLEPITPTKN NK VKVILGE DREATGVLLS IDGEDGIVRM DLDEQLKILN LRFLGKLLEA

UniProtKB: Transcription elongation factor SPT5

Macromolecule #2: Transcription elongation factor SPT4

• Macromolecule: Name: Transcription elongation factor SPT4 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.210201 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MALETVPKDL RHLRACLLCS LVKTIDQFEY DGCDNCDAYL QMKGNREMVY DCTSSSFDGI IAMMSPEDSW VSKWQRVSNF KPGVYAVSV TGRLPQGIVR ELKSRGVAYK SRDTAIKT

UniProtKB: Transcription elongation factor SPT4

Macromolecule #3: DNA-directed RNA polymerase subunit

• Macromolecule: Name: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)
• Molecular weight: Theoretical: 183.24425 KDa

Sequence

String:

SACPLRTIKR VQFGVLSPDE LKRMSVTEGG IKYPETTEGG RPKLGGLMDP RQGVIERTGR CQTCAGNMTE CPGHFGHIEL AKPVFHVGF LVKTMKVLRC VCFFCSKLLV DSNNPKIKDI LAKSKGQPKK RLTHVYDLCK GKNICEGGEE MDNKFGVEQP E GDEDLTKE KGHGGCGRYQ PRIRRSGLEL YAEWKHVNED SQEKKILLSP ERVHEIFKRI SDEECFVLGM EPRYARPEWM IV TVLPVPP LSVRPAVVMQ GSARNQDDLT HKLADIVKIN NQLRRNEQNG AAAHVIAEDV KLLQFHVATM VDNELPGLPR AMQ KSGRPL KSLKQRLKGK EGRVRGNLMG KRVDFSARTV ITPDPNLSID QVGVPRSIAA NMTFAEIVTP FNIDRLQELV RRGN SQYPG AKYIIRDNGD RIDLRFHPKP SDLHLQTGYK VERHMCDGDI VIFNRQPTLH KMSMMGHRVR ILPWSTFRLN LSVTT PYNA DFDGDEMNLH LPQSLETRAE IQELAMVPRM IVTPQSNRPV MGIVQDTLTA VRKFTKRDVF LERGEVMNLL MFLSTW DGK VPQPAILKPR PLWTGKQIFS LIIPGHINCI RTHSTHPDDE DSGPYKHISP GDTKVVVENG ELIMGILCKK SLGTSAG SL VHISYLEMGH DITRLFYSNI QTVINNWLLI EGHTIGIGDS IADSKTYQDI QNTIKKAKQD VIEVIEKAHN NELEPTPG N TLRQTFENQV NRILNDARDK TGSSAQKSLS EYNNFKSMVV SGAKGSKINI SQVIAVVGQQ NVEGKRIPFG FKHRTLPHF IKDDYGPESR GFVENSYLAG LTPTEFFFHA MGGREGLIDT AVKTAETGYI QRRLIKSMES VMVKYDATVR NSINQVVQLR YGEDGLAGE SVEFQNLATL KPSNKAFEKK FRFDYTNERA LRRTLQEDLV KDVLSNAHIQ NELEREFERM REDREVLRVI F PTGDSKVV LPCNLLRMIW NAQKIFHINP RLPSDLHPIK VVEGVKELSK KLVIVNGDDP LSRQAQENAT LLFNIHLRST LC SRRMAEE FRLSGEAFDW LLGEIESKFN QAIAHPGEMV GALAAQSLGE PATQMTLNTF HYAGVSAKNV TLGVPRLKEL INI SKKPKT PSLTVFLLGQ SARDAERAKD ILCRLEHTTL RKVTANTAIY YDPNPQSTVV AEDQEWVNVY YEMPDFDVAR ISPW LLRVE LDRKHMTDRK LTMEQIAEKI NAGFGDDLNC IFNDDNAEKL VLRIRIMNSD ENKMQEEEEV VDKMDDDVFL RCIES NMLT DMTLQGIEQI SKVYMHLPQT DNKKKIIITE DGEFKALQEW ILETDGVSLM RVLSEKDVDP VRTTSNDIVE IFTVLG IEA VRKALERELY HVISFDGSYV NYRHLALLCD TMTCRGHLMA ITRHGVNRQD TGPLMKCSFE ETVDVLMEAA AHGESDP MK GVSENIMLGQ LAPAGTGCFD LLLDAEKCKY GMEIPTNIPG LGAAGPTGMF FGSAPSPMGG ISPAMTPWNQ GATPAYGA W SPSVGSGMTP GAAGFSPSAA SDASGFSPGY SPAWSPTPGS PGSPGPSSPY IPSPGGAMSP SYSPTSPAYE PRSPGGYTP QSPSYSPTSP SYSPT(SEP)PSYS PTSPNYSPTS PSYSPT(SEP)PSY S

UniProtKB: DNA-directed RNA polymerase subunit

Macromolecule #5: Polymerase acidic protein

• Macromolecule: Name: Polymerase acidic protein / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: Hydrolases; Acting on ester bonds
• Source (natural): Organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
• Molecular weight: Theoretical: 82.91657 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

GMEDFVRQCF NPMIVELAEK AMKEYGEDPK IETNKFASIC THLEVCFMYS DFHFIDERGE STIIESGDPN VLLKHRFEII EGRDRTMAW TVVNSICNTT GVEKPKFLPD LYDYKENRFI DIGVTRREVH IYYLEKANKI KSEKTHIHIF SFTGEEMATK A DYTLDEES RARIKTRLFT IRQEMASRGL WDSFRQSERG EETIEERFEI TGTMRRLADQ SLPPNFSSLE NFRAYVDGFE PN GCIEGKL SQMSKEVNAR IEPFLRTTPR PLRLPDGPPC SQRSKFLLMD ALKLSIEDPS HEGEGIPLYD AIKCMKTFFG WKE PNIIKP HEKGINPNYL LTWKQVLAEL QDIENEEKIP RTKNMKKTSQ LKWALGENMA PEKVDFEDCK DVNDLKQYDS DEPE PRSLA CWIQSEFNKA CELTDSSWVE LDEIGEDVAP IEHIASMRRN YFTAEVSHCR ATEYIMKGVY INTALLNASC AAMDD FQLI PMISKCRTKE GRRKTNLYGF IIKGRSHLRN DTDVVNFVSM EFSLTDPRLE PHKWEKYCVL EIGDMLLRTA VGQVSR PMF LYVRTNGTSK IKMKWGMEMR RCLLQSLQQI ESMIEAESSV KEKDLTKEFF ENKSETWPIG ESPKGVEEGS IGKVCRT LL AKSVFNSLYA SPQLEGFSAE SRKLLLIVQA LRDNLEPGTF DLEGLYEAIE ECLINDPWVL LNASWFNSFL THALR

UniProtKB: Polymerase acidic protein

Macromolecule #6: RNA-directed RNA polymerase catalytic subunit

• Macromolecule: Name: RNA-directed RNA polymerase catalytic subunit / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA-directed RNA polymerase
• Source (natural): Organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
• Molecular weight: Theoretical: 86.496156 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MDVNPTLLFL KVPVQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHKYS EKGKWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVL EAMAFLEESH PGIFENSCLE TMEIVQQTRV DKLTQGRQTY DWTLNRNQPA ATALANTIEV FRSNGLTANE S GRLIDFLK DVMDSMDKEE MEITTHFQRK RRVRDNMTKK MVTQRTIGKK KQRLNKRSYL IRALTLNTMT KDAERGKLKR RA IATPGMQ IRGFVYFVEA LARSICEKLE QSGLPVGGNE KKAKLANVVR KMMTNSQDTE LSFTITGDNT KWNENQNPRM FLA MITYIT RNQPEWFRNV LSIAPIMFSN KMARLGKGYM FESKSMKLRT QVPAEMLANI DLKYFNKSTR EKIEKIRPLL IDGT ASLSP GMMMGMFNML STVLGVSILN LGQKKYTKTT YWWDGLQSSD DFALIVNAPN HEGIQAGVDR FYRTCKLVGI NMSKK KSYI NRTGTFEFTS FFYRYGFVAN FSMELPSFGV SGINESADMS VGVTVIKNNM INNDLGPATA QMALQLFIKD YRYTYR CHR GDTQIQTRRA FELKKLWEQT RSKAGLLVSD GGPNLYNIRN LHIPEVCLKW ELMDEDYQGR LCNPMNPFVS HKEIDSV NN AVVMPAHGPA KSMEYDAVAT THSWIPKRNR SILNTSQRGI LEDEQMYQKC CNLFEKFFPS SSYRRPVGIS SMVEAMVS R ARIDARIDFE SGRIKKEEFA EIMKICSTIE ELRRQK

UniProtKB: RNA-directed RNA polymerase catalytic subunit

Macromolecule #7: Polymerase basic protein 2

• Macromolecule: Name: Polymerase basic protein 2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
• Molecular weight: Theoretical: 86.00732 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MERIKELRDL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM MAMKYPITAD KRIMEMIPER NEQGQTLWSK TNDAGSDRV MVSPLAVTWW NRNGPTTSTV HYPKVYKTYF EKVERLKHGT FGPVHFRNQV KIRRRVDINP GHADLSAKEA Q DVIMEVVF PNEVGARILT SESQLTITKE KKKELQDCKI APLMVAYMLE RELVRKTRFL PVAGGTSSVY IEVLHLTQGT CW EQMYTPG GEVRNDDVDQ SLIIAARNIV RRATVSADPL ASLLEMCHST QIGGVRMVDI LRQNPTEEQA VDICKAAMGL RIS SSFSFG GFTFKRTSGS SVKREEEVLT GNLQTLKIRV HEGYEEFTMV GRRATAILRK ATRRLIQLIV SGKDEQSIAE AIIV AMVFS QEDCMIKAVR GDLNFVNRAN QRLNPMHQLL RHFQKDAKVL FQNWGIEPID NVMGMIGILP DMTPSTEMSL RGVRV SKMG VDEYSSTERV VVSIDRFLRV RDQRGNVLLS PEEVSETQGT EKLTITYSSS MMWEINGPES VLVNTYQWII RNWENV KIQ WSQDPTMLYN KMEFEPFQSL VPKAARGQYS GFVRVLFQQM RDVLGTFDTV QIIKLLPFAA APPEQSRMQF SSLTVNV RG SGMRIVVRGN SPVFNYNKAT KRLTVLGKDA GALMEDPDEG TAGVESAVLR GFLILGKENK RYGPALSINE LSNLAKGE K ANVLIGQGDV VLVMKRKRDS SILTDSQTAT KRIRMAIN

UniProtKB: Polymerase basic protein 2

Macromolecule #10: likely linker between KOWx4 and KOW5 domains of SPT5

• Macromolecule: Name: likely linker between KOWx4 and KOW5 domains of SPT5 / type: protein_or_peptide / ID: 10 / Details: register is based on first phenylalanine in linker / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 1.0892 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

AAAAAAFAAA AAAA

Macromolecule #11: DNA-directed RNA polymerase subunit beta

• Macromolecule: Name: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)
• Molecular weight: Theoretical: 134.041422 KDa

Sequence

String:

MYDADEDMQY DEDDDEITPD LWQEACWIVI SSYFDEKGLV RQQLDSFDEF IQMSVQRIVE DAPPIDLQAE AQHASGEVEE PPRYLLKFE QIYLSKPTHW ERDGAPSPMM PNEARLRNLT YSAPLYVDIT KTVIKEGEEQ LQTQHQKTFI GKIPIMLRST Y CLLNGLTD RDLCELNECP LDPGGYFIIN GSEKVLIAQE KMATNTVYVF AKKDSKYAYT GECRSCLENS SRPTSTIWVS ML ARGGQGA KKSAIGQRIV ATLPYIKQEV PIIIVFRALG FVSDRDILEH IIYDFEDPEM MEMVKPSLDE AFVIQEQNVA LNF IGSRGA KPGVTKEKRI KYAKEVLQKE MLPHVGVSDF CETKKAYFLG YMVHRLLLAA LGRRELDDRD HYGNKRLDLA GPLL AFLFR GMFKNLLKEV RIYAQKFIDR GKDFNLELAI KTRIISDGLK YSLATGNWGD QKKAHQARAG VSQVLNRLTF ASTLS HLRR LNSPIGRDGK LAKPRQLHNT LWGMVCPAET PEGHAVGLVK NLALMAYISV GSQPSPILEF LEEWSMENLE EISPAA IAD ATKIFVNGCW VGIHKDPEQL MNTLRKLRRQ MDIIVSEVSM IRDIREREIR IYTDAGRICR PLLIVEKQKL LLKKRHI DQ LKEREYNNYS WQDLVASGVV EYIDTLEEET VMLAMTPDDL QEKEVAYCST YTHCEIHPSM ILGVCASIIP FPDHNQSP R NTYQSAMGKQ AMGVYITNFH VRMDTLAHVL YYPQKPLVTT RSMEYLRFRE LPAGINSIVA IASYTGYNQE DSVIMNRSA VDRGFFRSVF YRSYKEQESK KGFDQEEVFE KPTRETCQGM RHAIYDKLDD DGLIAPGVRV SGDDVIIGKT VTLPENEDEL EGTNRRYTK RDCSTFLRTS ETGIVDQVMV TLNQEGYKFC KIRVRSVRIP QIGDKFASRH GQKGTCGIQY RQEDMPFTCE G ITPDIIIN PHAIPSRMTI GHLIECLQGK VSANKGEIGD ATPFNDAVNV QKISNLLSDY GYHLRGNEVL YNGFTGRKIT SQ IFIGPTY YQRLKHMVDD KIHSRARGPI QILNRQPMEG RSRDGGLRFG EMERDCQIAH GAAQFLRERL FEASDPYQVH VCN LCGIMA IANTRTHTYE CRGCRNKTQI SLVRMPYACK LLFQELMSMS IAPRMMSV

UniProtKB: DNA-directed RNA polymerase subunit beta

Macromolecule #12: DNA-directed RNA polymerase II subunit RPB3

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB3 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)
• Molecular weight: Theoretical: 31.439074 KDa

Sequence

String:

MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV QIDANSSVLH DEFIAHRLGL IPLTSDDIVD KLQYSRDCT CEEFCPECSV EFTLDVRCNE DQTRHVTSRD LISNSPRVIP VTSRNRDNDP SDYVEQDDIL IVKLRKGQEL R LRAYAKKG FGKEHAKWNP TAGVAFEYDP DNALRHTVYP KPEEWPKSEY SELDEDESQA PYDPNGKPER FYYNVESCGS LR PETIVLS ALSGLKKKLS DLQTQLSHEI QSDVLTIN

UniProtKB: DNA-directed RNA polymerase II subunit RPB3

Macromolecule #13: DNA-directed RNA polymerase II subunit E

• Macromolecule: Name: DNA-directed RNA polymerase II subunit E / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)
• Molecular weight: Theoretical: 24.644318 KDa

Sequence

String:

MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQFGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKV YCQRMQEENI TRALIVVQQG MTPSAKQSLV DMAPKYILEQ FLQQELLINI TEHELVPEHV VMTKEEVTEL L ARYKLREN QLPRIQAGDP VARYFGIKRG QVVKIIRPSE TAGRYITYRL VQ

UniProtKB: DNA-directed RNA polymerase II subunit E

Macromolecule #14: DNA-directed RNA polymerases I, II, and III subunit RPABC2

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC2; type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)
• Molecular weight: Theoretical: 14.477001 KDa

Sequence

String:

MSDNEDNFDG DDFDDVEEDE GLDDLENAEE EGQENVEILP SGERPQANQK RITTPYMTKY ERARVLGTRA LQIAMCAPVM VELEGETDP LLIAMKELKA RKIPIIIRRY LPDGSYEDWG VDELIISD

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC2

Macromolecule #15: DNA-directed RNA polymerases I, II, and III subunit RPABC3

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3; type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)
• Molecular weight: Theoretical: 17.162273 KDa

Sequence

String:

MAGILFEDIF DVKDIDPEGK KFDRVSRLHC ESESFKMDLI LDVNIQIYPV DLGDKFRLVI ASTLYEDGTL DDGEYNPTDD RPSRADQFE YVMYGKVYRI EGDETSTEAA TRLSAYVSYG GLLMRLQGDA NNLHGFEVDS RVYLLMKKLA F

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

Macromolecule #16: DNA-directed RNA polymerase II subunit RPB9

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB9 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)
• Molecular weight: Theoretical: 14.541221 KDa

Sequence

String:

MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN SCIYVNKITH EVDELTQIIA DVSQDPTLPR TEDHPCQKC GHKEAVFFQS HSARAEDAMR LYYVCTAPHC GHRWTE

UniProtKB: DNA-directed RNA polymerase II subunit RPB9

Macromolecule #17: DNA-directed RNA polymerases I, II, and III subunit RPABC5

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC5; type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)
• Molecular weight: Theoretical: 7.655123 KDa

Sequence

String:

MIIPVRCFTC GKIVGNKWEA YLGLLQAEYT EGDALDALGL KRYCCRRMLL AHVDLIEKLL NYAPLEK

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

Macromolecule #18: DNA-directed RNA polymerase II subunit RPB11-a

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB11-a / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)
• Molecular weight: Theoretical: 13.310284 KDa

Sequence

String:

MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTN AITDLISELS LLEERFRVAI KDKQEGIE

UniProtKB: DNA-directed RNA polymerase II subunit RPB11-a

Macromolecule #19: RNA polymerase II, I and III subunit K

• Macromolecule: Name: RNA polymerase II, I and III subunit K / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)
• Molecular weight: Theoretical: 7.018244 KDa

Sequence

String:

MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDAR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC4

Macromolecule #22: RNA polymerase II subunit D

• Macromolecule: Name: RNA polymerase II subunit D / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)
• Molecular weight: Theoretical: 20.962621 KDa

Sequence

String:

MWGPAQPYSD SALSPKPRPF RAVFRGSALP FPAVRVEVRG RSMAAGGSDP RAGDVEEDAS QLIFPKEFET AETLLNSEVH MLLEHRKQQ NESAEDEQEL SEVFMKTLNY TARFSRFKNR ETIASVRSLL LQKKLHKFEL ACLANLCPET AEESKALIPS L EGRFEDEE LQQILDDIQT KRSFQY

UniProtKB: RNA polymerase II subunit D

Macromolecule #23: DNA-directed RNA polymerase subunit

• Macromolecule: Name: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa domesticus (domestic pig)
• Molecular weight: Theoretical: 19.314283 KDa

Sequence

String:

MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVV TQVNKVGLFT EIGPMSCFIS RHSIPSEMEF DPNSNPPCYK TMDEDIVIQQ DDEIRLKIVG TRVDKNDIFA I GSLMDDYL GLVS

UniProtKB: DNA-directed RNA polymerase subunit

Macromolecule #4: 5' cap(1) RNA

• Macromolecule: Name: 5' cap(1) RNA / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: Influenza B virus
• Molecular weight: Theoretical: 11.437029 KDa

Sequence

String:

GAAGCGAGAA GAACACAGAC AGCAGCAGAC CAGGC

Macromolecule #8: 3' vRNA

• Macromolecule: Name: 3' vRNA / type: rna / ID: 8 / Number of copies: 1
• Source (natural): Organism: Influenza B virus
• Molecular weight: Theoretical: 4.008358 KDa

Sequence

String:

CUCUGCUUCU GCU

Macromolecule #9: 5' vRNA

• Macromolecule: Name: 5' vRNA / type: rna / ID: 9 / Number of copies: 1
• Source (natural): Organism: Influenza B virus
• Molecular weight: Theoretical: 4.55782 KDa

Sequence

String:

AGUAGUAACA AGAG

Macromolecule #20: nontemplate DNA (43-mer)

• Macromolecule: Name: nontemplate DNA (43-mer) / type: dna / ID: 20 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 13.303563 KDa

Sequence

String:

(DG)(DG)(DC)(DA)(DG)(DT)(DA)(DC)(DT)(DA) (DG)(DT)(DA)(DT)(DT)(DC)(DT)(DA)(DG)(DT) (DA)(DT)(DT)(DG)(DA)(DA)(DA)(DG)(DT) (DA)(DC)(DT)(DT)(DG)(DA)(DG)(DC)(DT)(DT) (DG) (DA)(DT)(DC)

Macromolecule #21: template DNA (43-mer)

• Macromolecule: Name: template DNA (43-mer) / type: dna / ID: 21 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 13.193495 KDa

Sequence

String:

(DG)(DA)(DT)(DC)(DA)(DA)(DG)(DC)(DT)(DC) (DA)(DA)(DG)(DT)(DA)(DC)(DT)(DT)(DA)(DA) (DG)(DT)(DC)(DT)(DG)(DG)(DT)(DC)(DT) (DA)(DT)(DA)(DC)(DT)(DA)(DG)(DT)(DA)(DC) (DT) (DG)(DC)(DC)

Macromolecule #24: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 24 / Number of copies: 8 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #25: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 25 / Number of copies: 2 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #26: 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-GUANOSINE

• Macromolecule: Name: 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-GUANOSINE; type: ligand / ID: 26 / Number of copies: 1 / Formula: G1G
• Molecular weight: Theoretical: 817.467 Da

Chemical component information

ChemComp-G1G:
7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-GUANOSINE

Macromolecule #27: PHOSPHATE ION

• Macromolecule: Name: PHOSPHATE ION / type: ligand / ID: 27 / Number of copies: 3 / Formula: PO4
• Molecular weight: Theoretical: 94.971 Da

Chemical component information

ChemComp-PO4:
PHOSPHATE ION

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 8 ; Details: 20 mM BICINE pH 8.0 150 mM NaCl 3 mM MgCl2 4% glycerol
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 95 % / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average exposure time: 2.4 sec. / Average electron dose: 40.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.2 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 11935228
• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: OTHER
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 63230
• Initial angle assignment: Type: ANGULAR RECONSTITUTION
• Final angle assignment: Type: ANGULAR RECONSTITUTION