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- EMDB-50513: 32nm repeat of the central pair complex 1 of the tubulin polyglut... -

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Entry
Database: EMDB / ID: EMD-50513
Title32nm repeat of the central pair complex 1 of the tubulin polyglutamylation deficient C.reinhardtii strain ttll9::NAT
Map data32nm repeat of the central pair complex 1 of the C.reinhardtii strain ttll9::NAT
Sample
  • Organelle or cellular component: Isolated axonemes from ttll9::NAT strain of C.reinhardtii
KeywordsCilia / axoneme / microtubule doublets / tubulin / STRUCTURAL PROTEIN
Biological speciesC.reinhardtii (plant)
Methodsubtomogram averaging / cryo EM / Resolution: 12.08 Å
AuthorsAlvarez Viar G / Pigino G
Funding supportEuropean Union, 1 items
OrganizationGrant numberCountry
European Research Council (ERC)819826European Union
CitationJournal: Curr Biol / Year: 2024
Title: Protofilament-specific nanopatterns of tubulin post-translational modifications regulate the mechanics of ciliary beating.
Authors: Gonzalo Alvarez Viar / Nikolai Klena / Fabrizio Martino / Adrian Pascal Nievergelt / Davide Bolognini / Paola Capasso / Gaia Pigino /
Abstract: Controlling ciliary beating is essential for motility and signaling in eukaryotes. This process relies on the regulation of various axonemal proteins that assemble in stereotyped patterns onto ...Controlling ciliary beating is essential for motility and signaling in eukaryotes. This process relies on the regulation of various axonemal proteins that assemble in stereotyped patterns onto individual microtubules of the ciliary structure. Additionally, each axonemal protein interacts exclusively with determined tubulin protofilaments of the neighboring microtubule to carry out its function. While it is known that tubulin post-translational modifications (PTMs) are important for proper ciliary motility, the mode and extent to which they contribute to these interactions remain poorly understood. Currently, the prevailing understanding is that PTMs can confer functional specialization at the level of individual microtubules. However, this paradigm falls short of explaining how the tubulin code can manage the complexity of the axonemal structure where functional interactions happen in defined patterns at the sub-microtubular scale. Here, we combine immuno-cryo-electron tomography (cryo-ET), expansion microscopy, and mutant analysis to show that, in motile cilia, tubulin glycylation and polyglutamylation form mutually exclusive protofilament-specific nanopatterns at a sub-microtubular scale. These nanopatterns are consistent with the distributions of axonemal dyneins and nexin-dynein regulatory complexes, respectively, and are indispensable for their regulation during ciliary beating. Our findings offer a new paradigm for understanding how different tubulin PTMs, such as glycylation, glutamylation, acetylation, tyrosination, and detyrosination, can coexist within the ciliary structure and specialize individual protofilaments for the regulation of diverse protein complexes. The identification of a ciliary tubulin nanocode by cryo-ET suggests the need for high-resolution studies to better understand the molecular role of PTMs in other cellular compartments beyond the cilium.
History
DepositionMay 31, 2024-
Header (metadata) releaseOct 2, 2024-
Map releaseOct 2, 2024-
UpdateOct 2, 2024-
Current statusOct 2, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_50513.png

Downloads & links

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Map

FileDownload / File: emd_50513.map.gz / Format: CCP4 / Size: 70.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation32nm repeat of the central pair complex 1 of the C.reinhardtii strain ttll9::NAT
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
6.04 Å/pix.
x 264 pix.
= 1594.56 Å		6.04 Å/pix.
x 264 pix.
= 1594.56 Å		6.04 Å/pix.
x 264 pix.
= 1594.56 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 6.04 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.013
Minimum - Maximum-0.017873289 - 0.05129385
Average (Standard dev.)-0.000110815105 (±0.006250006)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions264264264
Spacing264264264
CellA=B=C: 1594.5599 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Half map 2 of the 32nm repeat of...

Fileemd_50513_half_map_1.map
AnnotationHalf map 2 of the 32nm repeat of the central pair complex 1 of the C.reinhardtii strain ttll9::NAT
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map 1 of the 32nm repeat of...

Fileemd_50513_half_map_2.map
AnnotationHalf map 1 of the 32nm repeat of the central pair complex 1 of the C.reinhardtii strain ttll9::NAT
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Isolated axonemes from ttll9::NAT strain of C.reinhardtii

EntireName: Isolated axonemes from ttll9::NAT strain of C.reinhardtii
Components
  • Organelle or cellular component: Isolated axonemes from ttll9::NAT strain of C.reinhardtii

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Supramolecule #1: Isolated axonemes from ttll9::NAT strain of C.reinhardtii

SupramoleculeName: Isolated axonemes from ttll9::NAT strain of C.reinhardtii
type: organelle_or_cellular_component / ID: 1 / Parent: 0
Source (natural)Organism: C.reinhardtii (plant) / Strain: ttll9::NAT

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statefilament

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Sample preparation

BufferpH: 7.22
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 2.5 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.5 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 12.08 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 11623
ExtractionNumber tomograms: 85 / Number images used: 11623
Final angle assignmentType: MAXIMUM LIKELIHOOD

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