+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-50218 | ||||||||||||
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Title | Negative staining EM map for Mis18 core complex | ||||||||||||
Map data | |||||||||||||
Sample |
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Keywords | Centromere / CENP-A / Mis18 complex / Centromere Inheritance / Integrative structural analysis / CELL CYCLE | ||||||||||||
Function / homology | Function and homology information chromocenter / CENP-A containing chromatin assembly / cell communication / : / chromosome, centromeric region / Cajal body / Deposition of new CENPA-containing nucleosomes at the centromere / chromosome segregation / nuclear speck / cell cycle ...chromocenter / CENP-A containing chromatin assembly / cell communication / : / chromosome, centromeric region / Cajal body / Deposition of new CENPA-containing nucleosomes at the centromere / chromosome segregation / nuclear speck / cell cycle / cell division / intracellular membrane-bounded organelle / chromatin / DNA binding / nucleoplasm / identical protein binding / metal ion binding / nucleus / cytosol Similarity search - Function | ||||||||||||
Biological species | Homo sapiens (human) | ||||||||||||
Method | single particle reconstruction / negative staining / Resolution: 27.3 Å | ||||||||||||
Authors | Jeyaprakash AA / Medina-Pritchard B | ||||||||||||
Funding support | United Kingdom, European Union, 3 items
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Citation | Journal: to be published Title: Structural Basis for Mis18 Complex Assembly: Implications for Centromere Maintenance Authors: Jeyaprakash AA / Medina-Pritchard B | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_50218.map.gz | 85.8 MB | EMDB map data format | |
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Header (meta data) | emd-50218-v30.xml emd-50218.xml | 14.6 KB 14.6 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_50218_fsc.xml | 13.4 KB | Display | FSC data file |
Images | emd_50218.png | 17.9 KB | ||
Masks | emd_50218_msk_1.map | 91.1 MB | Mask map | |
Filedesc metadata | emd-50218.cif.gz | 4.7 KB | ||
Others | emd_50218_half_map_1.map.gz emd_50218_half_map_2.map.gz | 83.8 MB 84 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-50218 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-50218 | HTTPS FTP |
-Related structure data
Related structure data | 7sfyC 7sfzC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_50218.map.gz / Format: CCP4 / Size: 91.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.48 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_50218_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_50218_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_50218_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Mis18 alpha, Mis18 beta, Mis18BP1 20-130
Entire | Name: Mis18 alpha, Mis18 beta, Mis18BP1 20-130 |
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Components |
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-Supramolecule #1: Mis18 alpha, Mis18 beta, Mis18BP1 20-130
Supramolecule | Name: Mis18 alpha, Mis18 beta, Mis18BP1 20-130 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Homo sapiens (human) |
-Macromolecule #1: Mis18 alpha
Macromolecule | Name: Mis18 alpha / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: SNAAGVRSLR CSRGCAGGCE CGDKGKCSDS SLLGKRLSED SSRHQLLQKW ASMWSSMSED ASVADMERAQ LEEEAAAAEE RPLVFLCSGC RRPLGDSLSW VASQEDTNCI LLRCVSCNVS VDKEQKLSKR EKENGCVLET LCCAGCSLNL GYVYRCTPKN LDYKRDLFCL ...String: SNAAGVRSLR CSRGCAGGCE CGDKGKCSDS SLLGKRLSED SSRHQLLQKW ASMWSSMSED ASVADMERAQ LEEEAAAAEE RPLVFLCSGC RRPLGDSLSW VASQEDTNCI LLRCVSCNVS VDKEQKLSKR EKENGCVLET LCCAGCSLNL GYVYRCTPKN LDYKRDLFCL SVEAIESYVL GSSEKQIVSE DKELFNLESR VEIEKSLTQM EDVLKALQMK LWEAESKLSF ATCKS UniProtKB: Protein Mis18-alpha |
-Macromolecule #2: Mis18 beta
Macromolecule | Name: Mis18 beta / type: protein_or_peptide / ID: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: SNAAAQPLRH RSRCATPPRG DFCGGTERAI DQASFTTSME WDTQVVKGSS PLGPAGLGAE EPAAGPQLPS WLQPERCAVF QCAQCHAVLA DSVHLAWDLS RSLGAVVFSR VTNNVVLEAP FLVGIEGSLK GSTYNLLFCG SCGIPVGFHL YSTHAALAAL RGHFCLSSDK ...String: SNAAAQPLRH RSRCATPPRG DFCGGTERAI DQASFTTSME WDTQVVKGSS PLGPAGLGAE EPAAGPQLPS WLQPERCAVF QCAQCHAVLA DSVHLAWDLS RSLGAVVFSR VTNNVVLEAP FLVGIEGSLK GSTYNLLFCG SCGIPVGFHL YSTHAALAAL RGHFCLSSDK MVCYLLKTKA IVNASEMDIQ NVPLSEKIAE LKEKIVLTHN RLKSLMKILS EVTPDQSKPE N UniProtKB: Protein Mis18-beta |
-Macromolecule #3: Mis18BP1
Macromolecule | Name: Mis18BP1 / type: protein_or_peptide / ID: 3 / Details: 20-130 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: GPDSMQRRNL PMDAIFFDSI PSGTLTPVKD LVKYQNSSLK LNDHKKNQFL KMTTFNNKNI FQSTMLTEAT TSNSSLDISA IKPNKDGLKN KANYESPGKI FLRMKEKVLR DKQEQP UniProtKB: Mis18-binding protein 1 |
-Experimental details
-Structure determination
Method | negative staining |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Staining | Type: NEGATIVE / Material: 2% uranyl acetate |
-Electron microscopy
Microscope | FEI TECNAI F20 |
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Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm |
Image recording | Film or detector model: TVIPS TEMCAM-F816 (8k x 8k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | Model: Tecnai F20 / Image courtesy: FEI Company |