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- EMDB-50188: Gcn2 dimer bound to the 60S ribosomal subunit -

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基本情報

登録情報
データベース: EMDB / ID: EMD-50188
タイトルGcn2 dimer bound to the 60S ribosomal subunit
マップデータRELION PostProcess output, main map
試料
  • 複合体: 60S ribosomal subunit with bound Gcn2 dimer
    • タンパク質・ペプチド: x 42種
    • RNA: x 3種
  • リガンド: x 1種
キーワードLSU / 60S / Gcn2 / RIBOSOME
機能・相同性
機能・相同性情報


cellular response to histidine / regulation of cytoplasmic translational initiation in response to stress / positive regulation of translational initiation in response to starvation / GCN2-mediated signaling / eukaryotic translation initiation factor 2alpha kinase activity / negative regulation of translational initiation in response to stress / positive regulation of cellular response to amino acid starvation / regulation of translational initiation / regulation of polysaccharide biosynthetic process / transporter complex ...cellular response to histidine / regulation of cytoplasmic translational initiation in response to stress / positive regulation of translational initiation in response to starvation / GCN2-mediated signaling / eukaryotic translation initiation factor 2alpha kinase activity / negative regulation of translational initiation in response to stress / positive regulation of cellular response to amino acid starvation / regulation of translational initiation / regulation of polysaccharide biosynthetic process / transporter complex / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / protein kinase inhibitor activity / lipopolysaccharide transport / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / ribosomal large subunit binding / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / preribosome, large subunit precursor / L13a-mediated translational silencing of Ceruloplasmin expression / Gram-negative-bacterium-type cell outer membrane assembly / ribosomal large subunit export from nucleus / translational elongation / regulation of translational fidelity / protein-RNA complex assembly / maturation of LSU-rRNA / translational termination / translation initiation factor binding / cytosolic ribosome / cellular response to amino acid starvation / DNA damage checkpoint signaling / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / macroautophagy / translational initiation / modification-dependent protein catabolic process / protein tag activity / maintenance of translational fidelity / cell outer membrane / rRNA processing / ribosome biogenesis / double-stranded RNA binding / large ribosomal subunit / ribosome binding / protein autophosphorylation / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / protein phosphorylation / non-specific serine/threonine protein kinase / protein ubiquitination / protein kinase activity / negative regulation of translation / rRNA binding / intracellular signal transduction / structural constituent of ribosome / ribosome / translation / response to antibiotic / protein serine kinase activity / mRNA binding / ubiquitin protein ligase binding / nucleolus / protein homodimerization activity / RNA binding / zinc ion binding / ATP binding / nucleus / cytosol / cytoplasm
類似検索 - 分子機能
eIF-2-alpha kinase Gcn2 / Histidyl tRNA synthetase-related domain / Anticodon binding domain of tRNAs / RWD domain / RWD domain profile. / RWD / RWD domain / Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain / Histidyl-tRNA synthetase / : ...eIF-2-alpha kinase Gcn2 / Histidyl tRNA synthetase-related domain / Anticodon binding domain of tRNAs / RWD domain / RWD domain profile. / RWD / RWD domain / Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain / Histidyl-tRNA synthetase / : / Anticodon-binding domain superfamily / LptD, C-terminal / LPS-assembly protein LptD / : / LPS transport system D / Organic solvent tolerance-like, N-terminal / LptA/(LptD N-terminal domain) LPS transport protein / Group II dsDNA virus coat/capsid protein / : / Ubiquitin-conjugating enzyme/RWD-like / Ribosomal protein L41 / Ribosomal protein L41 / Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL) / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L10e / Ribosomal protein L44e signature. / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19, eukaryotic / Ribosomal protein L19e signature. / : / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L6e signature. / : / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal L18 C-terminal region / Ribosomal L40e family / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L23/L25, N-terminal / : / Ribosomal protein L23, N-terminal domain / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal Protein L6, KOW domain / Ribosomal protein L36e signature. / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / 60S ribosomal protein L19 / : / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L6e / Ribosomal protein L34Ae / Ribosomal protein L34e / Ribosomal protein L7A/L8 / 60S ribosomal protein L6E / 60S ribosomal protein L35 / Ribosomal protein L18e / Ribosomal protein L19e, C-terminal domain / Ribosomal_L19e / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L19/L19e / Ribosomal protein L7, eukaryotic / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L31e, conserved site / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e, N-terminal domain / Ribosomal protein L31e signature. / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / Ribosomal protein L37ae / Ribosomal L37ae protein family
類似検索 - ドメイン・相同性
Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A ...Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Large ribosomal subunit protein uL5A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL2A / Large ribosomal subunit protein eL18A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Small ribosomal subunit protein eS32B / Large ribosomal subunit protein uL4A / Putative major capsid protein p72 / Large ribosomal subunit protein uL3 / eIF-2-alpha kinase GCN2 / Large ribosomal subunit protein eL8A / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL13A / Large ribosomal subunit protein eL14A / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL34A / Large ribosomal subunit protein eL6A / Large ribosomal subunit protein eL21A / LPS-assembly protein LptD
類似検索 - 構成要素
生物種Saccharomyces cerevisiae (パン酵母)
手法単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.1 Å
データ登録者Paternoga H / Dimitrova-Paternoga L / Wilson DN
資金援助 ドイツ, 1件
OrganizationGrant number国
German Research Foundation (DFG)WI 3285/11-1 ドイツ
引用ジャーナル: Proc Natl Acad Sci U S A / 年: 2025
タイトル: Structure of a Gcn2 dimer in complex with the large 60S ribosomal subunit.
著者: Helge Paternoga / Lu Xia / Lyudmila Dimitrova-Paternoga / Sihan Li / Liewei L Yan / Malte Oestereich / Sergo Kasvandik / Ankanahalli N Nanjaraj Urs / Bertrand Beckert / Tanel Tenson / Hani ...著者: Helge Paternoga / Lu Xia / Lyudmila Dimitrova-Paternoga / Sihan Li / Liewei L Yan / Malte Oestereich / Sergo Kasvandik / Ankanahalli N Nanjaraj Urs / Bertrand Beckert / Tanel Tenson / Hani Zaher / Toshifumi Inada / Daniel N Wilson /
要旨: The integrated stress response (ISR) is a central signaling network that enables eukaryotic cells to respond to a variety of different environmental stresses. Such stresses cause ribosome collisions ...The integrated stress response (ISR) is a central signaling network that enables eukaryotic cells to respond to a variety of different environmental stresses. Such stresses cause ribosome collisions that lead to activation of the kinase Gcn2, resulting in the phosphorylation and inactivation of eukaryotic initiation factor 2 and thereby promoting selective translation of mRNAs to restore homeostasis. Despite the importance of the ISR and intensive study over the past decades, structural insight into how Gcn2 interacts with ribosomal particles has been lacking. Using ex vivo affinity purification approaches, we have obtained a cryoelectron microscopy structure of a yeast Gcn2 dimer in complex with the ribosomal 60S subunit. The Gcn2 dimer is formed by dimerization of the histidine tRNA synthetase-like domains, which establish extensive interactions with the stalk-base and sarcin-ricin loop of the 60S subunit. The C-terminal domain of Gcn2 is also dimerized and occupies the A- and P-site tRNA binding sites at the peptidyl-transferase center of the 60S subunit. Complementary functional studies indicate that binding of Gcn2 to the 60S subunit does not require the coactivators Gcn1 or Gcn20, nor does it lead to phosphorylation of eIF2α. Instead, upon stress, we observe a shift of Gcn2 from the 60S subunit into the colliding ribosome fraction, suggesting that the Gcn2-60S complex represents an inactive stand-by state to enable a rapid redistribution to collided ribosomes, and thereby facilitating a quick and efficient response to stress.
履歴
登録2024年4月28日-
ヘッダ(付随情報) 公開2025年3月26日-
マップ公開2025年3月26日-
更新2025年5月21日-
現状2025年5月21日処理サイト: PDBe / 状態: 公開

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構造の表示

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マップ

ファイルダウンロード / ファイル: emd_50188.map.gz / 形式: CCP4 / 大きさ: 216 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈RELION PostProcess output, main map
ボクセルのサイズX=Y=Z: 1.03 Å
密度
表面レベル登録者による: 0.0279
最小 - 最大-0.10073405 - 0.17432515
平均 (標準偏差)0.00023494453 (±0.006490925)
対称性空間群: 1
詳細

EMDB XML:

マップ形状
Axis orderXYZ
Origin000
サイズ384384384
Spacing384384384
セルA=B=C: 395.52 Å
α=β=γ: 90.0 °

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添付データ

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試料の構成要素

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全体 : 60S ribosomal subunit with bound Gcn2 dimer

全体名称: 60S ribosomal subunit with bound Gcn2 dimer
要素
  • 複合体: 60S ribosomal subunit with bound Gcn2 dimer
    • タンパク質・ペプチド: eIF-2-alpha kinase GCN2
    • タンパク質・ペプチド: Large ribosomal subunit protein eL41B
    • RNA: 5.8S rRNA
    • RNA: 5S rRNA
    • タンパク質・ペプチド: 60S ribosomal protein L3
    • タンパク質・ペプチド: 60S ribosomal protein L5
    • タンパク質・ペプチド: 60S ribosomal protein L6-A
    • タンパク質・ペプチド: 60S ribosomal protein L7-A
    • タンパク質・ペプチド: 60S ribosomal protein L8-A
    • タンパク質・ペプチド: 60S ribosomal protein L9-A
    • タンパク質・ペプチド: 60S ribosomal protein L10
    • タンパク質・ペプチド: 60S ribosomal protein L11-A
    • タンパク質・ペプチド: 60S ribosomal protein L13-A
    • タンパク質・ペプチド: 60S ribosomal protein L14-A
    • タンパク質・ペプチド: 60S ribosomal protein L15-A
    • タンパク質・ペプチド: 60S ribosomal protein L16-A
    • タンパク質・ペプチド: 60S ribosomal protein L17-A
    • タンパク質・ペプチド: 60S ribosomal protein L18-A
    • タンパク質・ペプチド: 60S ribosomal protein L19-A
    • タンパク質・ペプチド: 60S ribosomal protein L20-A
    • タンパク質・ペプチド: 60S ribosomal protein L21-A
    • タンパク質・ペプチド: 60S ribosomal protein L22-A
    • タンパク質・ペプチド: 60S ribosomal protein L23-A
    • タンパク質・ペプチド: 60S ribosomal protein L24-A
    • タンパク質・ペプチド: 60S ribosomal protein L25
    • タンパク質・ペプチド: 60S ribosomal protein L26-A
    • タンパク質・ペプチド: 60S ribosomal protein L27-A
    • タンパク質・ペプチド: 60S ribosomal protein L28
    • タンパク質・ペプチド: 60S ribosomal protein L29
    • タンパク質・ペプチド: 60S ribosomal protein L30
    • タンパク質・ペプチド: 60S ribosomal protein L31-A
    • タンパク質・ペプチド: 60S ribosomal protein L32
    • タンパク質・ペプチド: 60S ribosomal protein L33-A
    • タンパク質・ペプチド: 60S ribosomal protein L35-A
    • タンパク質・ペプチド: 60S ribosomal protein L36-A
    • タンパク質・ペプチド: 60S ribosomal protein L37-A
    • タンパク質・ペプチド: 60S ribosomal protein L38
    • タンパク質・ペプチド: 60S ribosomal protein L39
    • タンパク質・ペプチド: Ubiquitin-60S ribosomal protein L40
    • タンパク質・ペプチド: 60S ribosomal protein L42-A
    • タンパク質・ペプチド: 60S ribosomal protein L43-A
    • タンパク質・ペプチド: 60S ribosomal protein L4-A
    • タンパク質・ペプチド: 60S ribosomal protein L34-A
    • タンパク質・ペプチド: 60S ribosomal protein L2-A
    • RNA: 25S rRNA
  • リガンド: ZINC ION

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超分子 #1: 60S ribosomal subunit with bound Gcn2 dimer

超分子名称: 60S ribosomal subunit with bound Gcn2 dimer / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#45
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)

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分子 #1: eIF-2-alpha kinase GCN2

分子名称: eIF-2-alpha kinase GCN2 / タイプ: protein_or_peptide / ID: 1 / コピー数: 6 / 光学異性体: LEVO / EC番号: non-specific serine/threonine protein kinase
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 190.429406 KDa
配列文字列: MSLSHLTLDQ YYEIQCNELE AIRSIYMDDF TDLTKRKSSW DKQPQIIFEI TLRSVDKEPV ESSITLHFAM TPMYPYTAPE IEFKNVQNV MDSQLQMLKS EFKKIHNTSR GQEIIFEITS FTQEKLDEFQ NVVNTQSLED DRLQRIKETK EQLEKEEREK Q QETIKKRS ...文字列:
MSLSHLTLDQ YYEIQCNELE AIRSIYMDDF TDLTKRKSSW DKQPQIIFEI TLRSVDKEPV ESSITLHFAM TPMYPYTAPE IEFKNVQNV MDSQLQMLKS EFKKIHNTSR GQEIIFEITS FTQEKLDEFQ NVVNTQSLED DRLQRIKETK EQLEKEEREK Q QETIKKRS DEQRRIDEIV QRELEKRQDD DDDLLFNRTT QLDLQPPSEW VASGEAIVFS KTIKAKLPNN SMFKFKAVVN PK PIKLTSD IFSFSKQFLV KPYIPPESPL ADFLMSSEMM ENFYYLLSEI ELDNSYFNTS NGKKEIANLE KELETVLKAK HDN VNRLFG YTVERMGRNN ATFVWKIRLL TEYCNYYPLG DLIQSVGFVN LATARIWMIR LLEGLEAIHK LGIVHKCINL ETVI LVKDA DFGSTIPKLV HSTYGYTVLN MLSRYPNKNG SSVELSPSTW IAPELLKFNN AKPQRLTDIW QLGVLFIQII SGSDI VMNF ETPQEFLDST SMDETLYDLL SKMLNNDPKK RLGTLELLPM KFLRTNIDST INRFNLVSES VNSNSLELTP GDTITV RGN GGRTLSQSSI RRRSFNVGSR FSSINPATRS RYASDFEEIA VLGQGAFGQV VKARNALDSR YYAIKKIRHT EEKLSTI LS EVMLLASLNH QYVVRYYAAW LEEDSMDENV FESTDEESDL SESSSDFEEN DLLDQSSIFK NRTNHDLDNS NWDFISGS G YPDIVFENSS RDDENEDLDH DTSSTSSSES QDDTDKESKS IQNVPRRRNF VKPMTAVKKK STLFIQMEYC ENRTLYDLI HSENLNQQRD EYWRLFRQIL EALSYIHSQG IIHRDLKPMN IFIDESRNVK IGDFGLAKNV HRSLDILKLD SQNLPGSSDN LTSAIGTAM YVATEVLDGT GHYNEKIDMY SLGIIFFEMI YPFSTGMERV NILKKLRSVS IEFPPDFDDN KMKVEKKIIR L LIDHDPNK RPGARTLLNS GWLPVKHQDE VIKEALKSLS NPSSPWQQQV RESLFNQSYS LTNDILFDNS VPTSTPFANI LR SQMTEEV VKIFRKHGGI ENNAPPRIFP KAPIYGTQNV YEVLDKGGTV LQLQYDLTYP MARYLSKNPS LISKQYRMQH VYR PPDHSR SSLEPRKFGE IDFDIISKSS SESGFYDAES LKIIDEILTV FPVFEKTNTF FILNHADILE SVFNFTNIDK AQRP LVSRM LSQVGFARSF KEVKNELKAQ LNISSTALND LELFDFRLDF EAAKKRLYKL MIDSPHLKKI EDSLSHISKV LSYLK PLEV ARNVVISPLS NYNSAFYKGG IMFHAVYDDG SSRNMIAAGG RYDTLISFFA RPSGKKSSNT RKAVGFNLAW ETIFGI AQN YFKLASGNRI KKRNRFLKDT AVDWKPSRCD VLISSFSNSL LDTIGVTILN TLWKQNIKAD MLRDCSSVDD VVTGAQQ DG IDWILLIKQQ AYPLTNHKRK YKPLKIKKLS TNVDIDLDLD EFLTLYQQET GNKSLINDSL TLGDKADEFK RWDENSSA G SSQEGDIDDV VAGSTNNQKV IYVPNMATRS KKANKREKWV YEDAARNSSN MILHNLSNAP IITVDALRDE TLEIISITS LAQKEEWLRK VFGSGNNSTP RSFATSIYNN LSKEAHKGNR WAILYCHKTG KSSVIDLQR

UniProtKB: eIF-2-alpha kinase GCN2

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分子 #2: Large ribosomal subunit protein eL41B

分子名称: Large ribosomal subunit protein eL41B / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 3.354243 KDa
配列文字列:
MRAKWRKKRT RRLKRKRRKV RARSK

UniProtKB: Small ribosomal subunit protein eS32B

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分子 #5: 60S ribosomal protein L3

分子名称: 60S ribosomal protein L3 / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 43.850793 KDa
配列文字列: MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK ...文字列:
MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK TPLAQKKAHL AEIQLNGGSI SEKVDWAREH FEKTVAVDSV FEQNEMIDAI AVTKGHGFEG VTHRWGTKKL PR KTHRGLR KVACIGAWHP AHVMWSVARA GQRGYHSRTS INHKIYRVGK GDDEANGATS FDRTKKTITP MGGFVHYGEI KND FIMVKG CIPGNRKRIV TLRKSLYTNT SRKALEEVSL KWIDTASKFG KGRFQTPAEK HAFMGTLKKD L

UniProtKB: Large ribosomal subunit protein uL3

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分子 #6: 60S ribosomal protein L5

分子名称: 60S ribosomal protein L5 / タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 33.764828 KDa
配列文字列: MAFQKDAKSS AYSSRFQTPF RRRREGKTDY YQRKRLVTQH KAKYNTPKYR LVVRFTNKDI ICQIISSTIT GDVVLAAAYS HELPRYGIT HGLTNWAAAY ATGLLIARRT LQKLGLDETY KGVEEVEGEY ELTEAVEDGP RPFKVFLDIG LQRTTTGARV F GALKGASD ...文字列:
MAFQKDAKSS AYSSRFQTPF RRRREGKTDY YQRKRLVTQH KAKYNTPKYR LVVRFTNKDI ICQIISSTIT GDVVLAAAYS HELPRYGIT HGLTNWAAAY ATGLLIARRT LQKLGLDETY KGVEEVEGEY ELTEAVEDGP RPFKVFLDIG LQRTTTGARV F GALKGASD GGLYVPHSEN RFPGWDFETE EIDPELLRSY IFGGHVSQYM EELADDDEER FSELFKGYLA DDIDADSLED IY TSAHEAI RADPAFKPTE KKFTKEQYAA ESKKYRQTKL SKEERAARVA AKIAALAGQQ

UniProtKB: Large ribosomal subunit protein uL18

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分子 #7: 60S ribosomal protein L6-A

分子名称: 60S ribosomal protein L6-A / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 20.000564 KDa
配列文字列:
MSAQKAPKWY PSEDVAALKK TRKAARPQKL RASLVPGTVL ILLAGRFRGK RVVYLKHLED NTLLISGPFK VNGVPLRRVN ARYVIATST KVSVEGVNVE KFNVEYFAKE KLTKKEKKEA NLFPEQQNKE IKAERVEDQK VVDKALIAEI KKTPLLKQYL S ASFSLKNG DKPHMLKF

UniProtKB: Large ribosomal subunit protein eL6A

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分子 #8: 60S ribosomal protein L7-A

分子名称: 60S ribosomal protein L7-A / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 27.686281 KDa
配列文字列: MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI ...文字列:
MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI IEANLGKYGI LSIDDLIHEI ITVGPHFKQA NNFLWPFKLS NPSGGWGVPR KFKHFIQGGS FGNREEFINK LV KSMN

UniProtKB: Large ribosomal subunit protein uL30A

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分子 #9: 60S ribosomal protein L8-A

分子名称: 60S ribosomal protein L8-A / タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 28.17582 KDa
配列文字列: MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA ...文字列:
MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA LCKKMGVPYA IVKGKARLGT LVNQKTSAVA ALTEVRAEDE AALAKLVSTI DANFADKYDE VKKHWGGGIL GN KAQAKMD KRAKNSDSA

UniProtKB: Large ribosomal subunit protein eL8A

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分子 #10: 60S ribosomal protein L9-A

分子名称: 60S ribosomal protein L9-A / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 21.605061 KDa
配列文字列: MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR ...文字列:
MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

UniProtKB: Large ribosomal subunit protein uL6A

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分子 #11: 60S ribosomal protein L10

分子名称: 60S ribosomal protein L10 / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 25.410465 KDa
配列文字列: MARRPARCYR YQKNKPYPKS RYNRAVPDSK IRIYDLGKKK ATVDEFPLCV HLVSNELEQL SSEALEAARI CANKYMTTVS GRDAFHLRV RVHPFHVLRI NKMLSCAGAD RLQQGMRGAW GKPHGLAARV DIGQIIFSVR TKDSNKDVVV EGLRRARYKF P GQQKIILS ...文字列:
MARRPARCYR YQKNKPYPKS RYNRAVPDSK IRIYDLGKKK ATVDEFPLCV HLVSNELEQL SSEALEAARI CANKYMTTVS GRDAFHLRV RVHPFHVLRI NKMLSCAGAD RLQQGMRGAW GKPHGLAARV DIGQIIFSVR TKDSNKDVVV EGLRRARYKF P GQQKIILS KKWGFTNLDR PEYLKKREAG EVKDDGAFVK FLSKKGSLEN NIREFPEYFA AQA

UniProtKB: Large ribosomal subunit protein uL16

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分子 #12: 60S ribosomal protein L11-A

分子名称: 60S ribosomal protein L11-A / タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 19.755691 KDa
配列文字列:
MSAKAQNPMR DLKIEKLVLN ISVGESGDRL TRASKVLEQL SGQTPVQSKA RYTVRTFGIR RNEKIAVHVT VRGPKAEEIL ERGLKVKEY QLRDRNFSAT GNFGFGIDEH IDLGIKYDPS IGIFGMDFYV VMNRPGARVT RRKRCKGTVG NSHKTTKEDT V SWFKQKYD ADVLDK

UniProtKB: Large ribosomal subunit protein uL5A

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分子 #13: 60S ribosomal protein L13-A

分子名称: 60S ribosomal protein L13-A / タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 22.604164 KDa
配列文字列: MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR ...文字列:
MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR TLRLARSEKK FRGIREKRAR EKAEAEAEKK K

UniProtKB: LPS-assembly protein LptD

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分子 #14: 60S ribosomal protein L14-A

分子名称: 60S ribosomal protein L14-A / タイプ: protein_or_peptide / ID: 14 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 15.195066 KDa
配列文字列:
MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK AGVPRQAINL GQVVLTPLTF ALPRGARTAT VSKKWAAAA VCEKWAASSW AKKIAQRERR AALTDFERFQ VMVLRKQKRY TVKKALAKA

UniProtKB: Large ribosomal subunit protein eL14A

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分子 #15: 60S ribosomal protein L15-A

分子名称: 60S ribosomal protein L15-A / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 24.482357 KDa
配列文字列: MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVPKGAT YGKPTNQGV NELKYQRSLR ATAEERVGRR AANLRVLNSY WVNQDSTYKY FEVILVDPQH KAIRRDARYN WICDPVHKHR E ARGLTATG ...文字列:
MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVPKGAT YGKPTNQGV NELKYQRSLR ATAEERVGRR AANLRVLNSY WVNQDSTYKY FEVILVDPQH KAIRRDARYN WICDPVHKHR E ARGLTATG KKSRGINKGH KFNNTKAGRR KTWKRQNTLS LWRYRK

UniProtKB: Large ribosomal subunit protein eL15A

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分子 #16: 60S ribosomal protein L16-A

分子名称: 60S ribosomal protein L16-A / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 22.247227 KDa
配列文字列: MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY ...文字列:
MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY AKKRAFTKKV ASANATAAES DVAKQLAALG Y

UniProtKB: Large ribosomal subunit protein uL13A

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分子 #17: 60S ribosomal protein L17-A

分子名称: 60S ribosomal protein L17-A / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 20.589518 KDa
配列文字列:
MARYGATSTN PAKSASARGS YLRVSFKNTR ETAQAINGWE LTKAQKYLEQ VLDHQRAIPF RRFNSSIGRT AQGKEFGVTK ARWPAKSVK FVQGLLQNAA ANAEAKGLDA TKLYVSHIQV NQAPKQRRRT YRAHGRINKY ESSPSHIELV VTEKEEAVAK A AEKKVVRL TSRQRGRIAA QKRIAA

UniProtKB: Large ribosomal subunit protein uL22A

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分子 #18: 60S ribosomal protein L18-A

分子名称: 60S ribosomal protein L18-A / タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 20.609252 KDa
配列文字列:
MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK ALFLSKINRP PVSVSRIARA LKQEGAANKT VVVVGTVTD DARIFEFPKT TVAALRFTAG ARAKIVKAGG ECITLDQLAV RAPKGQNTLI LRGPRNSREA VRHFGMGPHK G KAPRILST GRKFERARGR RRSKGFKV

UniProtKB: Large ribosomal subunit protein eL18A

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分子 #19: 60S ribosomal protein L19-A

分子名称: 60S ribosomal protein L19-A / タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 21.762316 KDa
配列文字列: MANLRTQKRL AASVVGVGKR KVWLDPNETS EIAQANSRNA IRKLVKNGTI VKKAVTVHSK SRTRAHAQSK REGRHSGYGK RKGTREARL PSQVVWIRRL RVLRRLLAKY RDAGKIDKHL YHVLYKESKG NAFKHKRALV EHIIQAKADA QREKALNEEA E ARRLKNRA ...文字列:
MANLRTQKRL AASVVGVGKR KVWLDPNETS EIAQANSRNA IRKLVKNGTI VKKAVTVHSK SRTRAHAQSK REGRHSGYGK RKGTREARL PSQVVWIRRL RVLRRLLAKY RDAGKIDKHL YHVLYKESKG NAFKHKRALV EHIIQAKADA QREKALNEEA E ARRLKNRA ARDRRAQRVA EKRDALLKED A

UniProtKB: Large ribosomal subunit protein eL19A

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分子 #20: 60S ribosomal protein L20-A

分子名称: 60S ribosomal protein L20-A / タイプ: protein_or_peptide / ID: 20 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 20.478852 KDa
配列文字列:
MAHFKEYQVI GRRLPTESVP EPKLFRMRIF ASNEVIAKSR YWYFLQKLHK VKKASGEIVS INQINEAHPT KVKNFGVWVR YDSRSGTHN MYKEIRDVSR VAAVETLYQD MAARHRARFR SIHILKVAEI EKTADVKRQY VKQFLTKDLK FPLPHRVQKS T KTFSYKRP STFY

UniProtKB: Large ribosomal subunit protein eL20A

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分子 #21: 60S ribosomal protein L21-A

分子名称: 60S ribosomal protein L21-A / タイプ: protein_or_peptide / ID: 21 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 18.279266 KDa
配列文字列:
MGKSHGYRSR TRYMFQRDFR KHGAVHLSTY LKVYKVGDIV DIKANGSIQK GMPHKFYQGK TGVVYNVTKS SVGVIINKMV GNRYLEKRL NLRVEHIKHS KCRQEFLERV KANAAKRAEA KAQGVAVQLK RQPAQPRESR IVSTEGNVPQ TLAPVPYETF I

UniProtKB: Large ribosomal subunit protein eL21A

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分子 #22: 60S ribosomal protein L22-A

分子名称: 60S ribosomal protein L22-A / タイプ: protein_or_peptide / ID: 22 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 13.711359 KDa
配列文字列:
MAPNTSRKQK IAKTFTVDVS SPTENGVFDP ASYAKYLIDH IKVEGAVGNL GNAVTVTEDG TVVTVVSTAK FSGKYLKYLT KKYLKKNQL RDWIRFVSTK TNEYRLAFYQ VTPEEDEEED EE

UniProtKB: Large ribosomal subunit protein eL22A

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分子 #23: 60S ribosomal protein L23-A

分子名称: 60S ribosomal protein L23-A / タイプ: protein_or_peptide / ID: 23 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 14.49395 KDa
配列文字列:
MSGNGAQGTK FRISLGLPVG AIMNCADNSG ARNLYIIAVK GSGSRLNRLP AASLGDMVMA TVKKGKPELR KKVMPAIVVR QAKSWRRRD GVFLYFEDNA GVIANPKGEM KGSAITGPVG KECADLWPRV ASNSGVVV

UniProtKB: Large ribosomal subunit protein uL14A

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分子 #24: 60S ribosomal protein L24-A

分子名称: 60S ribosomal protein L24-A / タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 17.661717 KDa
配列文字列:
MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK KGITEEVAKK RSRKTVKAQR PITGASLDL IKERRSLKPE VRKANREEKL KANKEKKKAE KAARKAEKAK SAGTQSSKFS KQQAKGAFQK VAATSR

UniProtKB: Large ribosomal subunit protein eL24A

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分子 #25: 60S ribosomal protein L25

分子名称: 60S ribosomal protein L25 / タイプ: protein_or_peptide / ID: 25 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 15.787612 KDa
配列文字列:
MAPSAKATAA KKAVVKGTNG KKALKVRTSA TFRLPKTLKL ARAPKYASKA VPHYNRLDSY KVIEQPITSE TAMKKVEDGN ILVFQVSMK ANKYQIKKAV KELYEVDVLK VNTLVRPNGT KKAYVRLTAD YDALDIANRI GYI

UniProtKB: Large ribosomal subunit protein uL23

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分子 #26: 60S ribosomal protein L26-A

分子名称: 60S ribosomal protein L26-A / タイプ: protein_or_peptide / ID: 26 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 14.265784 KDa
配列文字列:
MAKQSLDVSS DRRKARKAYF TAPSSQRRVL LSAPLSKELR AQYGIKALPI RRDDEVLVVR GSKKGQEGKI SSVYRLKFAV QVDKVTKEK VNGASVPINL HPSKLVITKL HLDKDRKALI QRKGGKLE

UniProtKB: Large ribosomal subunit protein uL24A

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分子 #27: 60S ribosomal protein L27-A

分子名称: 60S ribosomal protein L27-A / タイプ: protein_or_peptide / ID: 27 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 15.56836 KDa
配列文字列:
MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGSKSHPFGH ALVAGIERYP LKVTKKHGAK KVAKRTKIKP FIKVVNYNHL LPTRYTLDV EAFKSVVSTE TFEQPSQREE AKKVVKKAFE ERHQAGKNQW FFSKLRF

UniProtKB: Large ribosomal subunit protein eL27A

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分子 #28: 60S ribosomal protein L28

分子名称: 60S ribosomal protein L28 / タイプ: protein_or_peptide / ID: 28 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 16.761666 KDa
配列文字列:
MPSRFTKTRK HRGHVSAGKG RIGKHRKHPG GRGMAGGQHH HRINMDKYHP GYFGKVGMRY FHKQQAHFWK PVLNLDKLWT LIPEDKRDQ YLKSASKETA PVIDTLAAGY GKILGKGRIP NVPVIVKARF VSKLAEEKIR AAGGVVELIA

UniProtKB: Large ribosomal subunit protein uL15

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分子 #29: 60S ribosomal protein L29

分子名称: 60S ribosomal protein L29 / タイプ: protein_or_peptide / ID: 29 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 6.691884 KDa
配列文字列:
MAKSKNHTAH NQTRKAHRNG IKKPKTYKYP SLKGVDPKFR RNHKHALHGT AKALAAAKK

UniProtKB: Large ribosomal subunit protein eL29

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分子 #30: 60S ribosomal protein L30

分子名称: 60S ribosomal protein L30 / タイプ: protein_or_peptide / ID: 30 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 11.430364 KDa
配列文字列:
MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV LRKSELEYYA MLSKTKVYYF QGGNNELGTA VGKLFRVGV VSILEAGDSD ILTTLA

UniProtKB: Putative major capsid protein p72

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分子 #31: 60S ribosomal protein L31-A

分子名称: 60S ribosomal protein L31-A / タイプ: protein_or_peptide / ID: 31 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 12.980158 KDa
配列文字列:
MAGLKDVVTR EYTINLHKRL HGVSFKKRAP RAVKEIKKFA KLHMGTDDVR LAPELNQAIW KRGVKGVEYR LRLRISRKRN EEEDAKNPL FSYVEPVLVA SAKGLQTVVV EEDA

UniProtKB: Large ribosomal subunit protein eL31A

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分子 #32: 60S ribosomal protein L32

分子名称: 60S ribosomal protein L32 / タイプ: protein_or_peptide / ID: 32 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 14.809441 KDa
配列文字列:
MASLPHPKIV KKHTKKFKRH HSDRYHRVAE NWRKQKGIDS VVRRRFRGNI SQPKIGYGSN KKTKFLSPSG HKTFLVANVK DLETLTMHT KTYAAEIAHN ISAKNRVVIL ARAKALGIKV TNPKGRLALE A

UniProtKB: Large ribosomal subunit protein eL32

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分子 #33: 60S ribosomal protein L33-A

分子名称: 60S ribosomal protein L33-A / タイプ: protein_or_peptide / ID: 33 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 12.17713 KDa
配列文字列:
MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA YVYRASKEVR GSKIRVMWGK VTRTHGNSGV VRATFRNNL PAKTFGASVR IFLYPSNI

UniProtKB: Large ribosomal subunit protein eL33A

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分子 #34: 60S ribosomal protein L35-A

分子名称: 60S ribosomal protein L35-A / タイプ: protein_or_peptide / ID: 34 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 13.94264 KDa
配列文字列:
MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALR RALTKFEASQ VTEKQRKKQI AFPQRKYAIK A

UniProtKB: Large ribosomal subunit protein uL29A

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分子 #35: 60S ribosomal protein L36-A

分子名称: 60S ribosomal protein L36-A / タイプ: protein_or_peptide / ID: 35 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 11.151259 KDa
配列文字列:
MTVKTGIAIG LNKGKKVTSM TPAPKISYKK GAASNRTKFV RSLVREIAGL SPYERRLIDL IRNSGEKRAR KVAKKRLGSF TRAKAKVEE MNNIIAASRR H

UniProtKB: Large ribosomal subunit protein eL36A

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分子 #36: 60S ribosomal protein L37-A

分子名称: 60S ribosomal protein L37-A / タイプ: protein_or_peptide / ID: 36 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 9.877395 KDa
配列文字列:
MGKGTPSFGK RHNKSHTLCN RCGRRSFHVQ KKTCSSCGYP AAKTRSYNWG AKAKRRHTTG TGRMRYLKHV SRRFKNGFQT GSASKASA

UniProtKB: Large ribosomal subunit protein eL37A

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分子 #37: 60S ribosomal protein L38

分子名称: 60S ribosomal protein L38 / タイプ: protein_or_peptide / ID: 37 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 8.845561 KDa
配列文字列:
MAREITDIKQ FLELTRRADV KTATVKINKK LNKAGKPFRQ TKFKVRGSSS LYTLVINDAG KAKKLIQSLP PTLKVNRL

UniProtKB: Large ribosomal subunit protein eL38

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分子 #38: 60S ribosomal protein L39

分子名称: 60S ribosomal protein L39 / タイプ: protein_or_peptide / ID: 38 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 6.35864 KDa
配列文字列:
MAAQKSFRIK QKMAKAKKQN RPLPQWIRLR TNNTIRYNAK RRNWRRTKMN I

UniProtKB: Large ribosomal subunit protein eL39

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分子 #39: Ubiquitin-60S ribosomal protein L40

分子名称: Ubiquitin-60S ribosomal protein L40 / タイプ: protein_or_peptide / ID: 39 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 14.583077 KDa
配列文字列:
MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLKALASKY NCDKSVCRKC YARLPPRATN CRKRKCGHTN QLRPKKKLK

UniProtKB: Ubiquitin-ribosomal protein eL40A fusion protein

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分子 #40: 60S ribosomal protein L42-A

分子名称: 60S ribosomal protein L42-A / タイプ: protein_or_peptide / ID: 40 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 12.246658 KDa
配列文字列:
MVNVPKTRKT YCKGKTCRKH TQHKVTQYKA GKASLFAQGK RRYDRKQSGF GGQTKPVFHK KAKTTKKVVL RLECVKCKTR AQLTLKRCK HFELGGEKKQ KGQALQF

UniProtKB: Large ribosomal subunit protein eL42A

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分子 #41: 60S ribosomal protein L43-A

分子名称: 60S ribosomal protein L43-A / タイプ: protein_or_peptide / ID: 41 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 10.112952 KDa
配列文字列:
MAKRTKKVGI TGKYGVRYGS SLRRQVKKLE IQQHARYDCS FCGKKTVKRG AAGIWTCSCC KKTVAGGAYT VSTAAAATVR STIRRLREM VEA

UniProtKB: Large ribosomal subunit protein eL43A

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分子 #42: 60S ribosomal protein L4-A

分子名称: 60S ribosomal protein L4-A / タイプ: protein_or_peptide / ID: 42 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 39.159125 KDa
配列文字列: MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA ...文字列:
MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA LKAVGAHSDL LKVLKSKKLR AGKGKYRNRR WTQRRGPLVV YAEDNGIVKA LRNVPGVETA NVASLNLLQL AP GAHLGRF VIWTEAAFTK LDQVWGSETV ASSKVGYTLP SHIISTSDVT RIINSSEIQS AIRPAGQATQ KRTHVLKKNP LKN KQVLLR LNPYAKVFAA EKLGSKKAEK TGTKPAAVFT ETLKHD

UniProtKB: Large ribosomal subunit protein uL4A

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分子 #43: 60S ribosomal protein L34-A

分子名称: 60S ribosomal protein L34-A / タイプ: protein_or_peptide / ID: 43 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 13.673196 KDa
配列文字列:
MAQRVTFRRR NPYNTRSNKI KVVKTPGGIL RAQHVKKLAT RPKCGDCGSA LQGISTLRPR QYATVSKTHK TVSRAYGGSR CANCVKERI IRAFLIEEQK IVKKVVKEQT EAAKKSEKKA KK

UniProtKB: Large ribosomal subunit protein eL34A

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分子 #44: 60S ribosomal protein L2-A

分子名称: 60S ribosomal protein L2-A / タイプ: protein_or_peptide / ID: 44 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 27.463574 KDa
配列文字列: MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIY AGKKASLNVG NVLPLGSVPE GTIVSNVEEK PGDRGALARA SGNYVIIIGH NPDENKTRVR LPSGAKKVIS S DARGVIGV ...文字列:
MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIY AGKKASLNVG NVLPLGSVPE GTIVSNVEEK PGDRGALARA SGNYVIIIGH NPDENKTRVR LPSGAKKVIS S DARGVIGV IAGGGRVDKP LLKAGRAFHK YRLKRNSWPK TRGVAMNPVD HPHGGGNHQH IGKASTISRG AVSGQKAGLI AA RRTGLLR GSQKTQD

UniProtKB: Large ribosomal subunit protein uL2A

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分子 #3: 5.8S rRNA

分子名称: 5.8S rRNA / タイプ: rna / ID: 3 / コピー数: 1
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 50.682922 KDa
配列文字列:
AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

GENBANK: GENBANK: MG817620.1

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分子 #4: 5S rRNA

分子名称: 5S rRNA / タイプ: rna / ID: 4 / コピー数: 1
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 38.951105 KDa
配列文字列:
GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

GENBANK: GENBANK: CP022977.1

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分子 #45: 25S rRNA

分子名称: 25S rRNA / タイプ: rna / ID: 45 / コピー数: 1
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 1.0974938749999998 MDa
配列文字列: GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU ...文字列:
GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU UGGGGCCGUU CCUUGUCUAU GUUCCUUGGA ACAGGACGUC AUAGAGGGUG AGAAUCCCGU GUGGCGAGGA GU GCGGUUC UUUGUAAAGU GCCUUCGAAG AGUCGAGUUG UUUGGGAAUG CAGCUCUAAG UGGGUGGUAA AUUCCAUCUA AAG CUAAAU AUUGGCGAGA GACCGAUAGC GAACAAGUAC AGUGAUGGAA AGAUGAAAAG AACUUUGAAA AGAGAGUGAA AAAG UACGU GAAAUUGUUG AAAGGGAAGG GCAUUUGAUC AGACAUGGUG UUUUGUGCCC UCUGCUCCUU GUGGGUAGGG GAAUC UCGC AUUUCACUGG GCCAGCAUCA GUUUUGGUGG CAGGAUAAAU CCAUAGGAAU GUAGCUUGCC UCGGUAAGUA UUAUAG CCU GUGGGAAUAC UGCCAGCUGG GACUGAGGAC UGCGACGUAA GUCAAGGAUG CUGGCAUAAU GGUUAUAUGC CGCCCGU CU UGAAACACGG ACCAAGGAGU CUAACGUCUA UGCGAGUGUU UGGGUGUAAA ACCCAUACGC GUAAUGAAAG UGAACGUA G GUUGGGGCCU CGCAAGAGGU GCACAAUCGA CCGAUCCUGA UGUCUUCGGA UGGAUUUGAG UAAGAGCAUA GCUGUUGGG ACCCGAAAGA UGGUGAACUA UGCCUGAAUA GGGUGAAGCC AGAGGAAACU CUGGUGGAGG CUCGUAGCGG UUCUGACGUG CAAAUCGAU CGUCGAAUUU GGGUAUAGGG GCGAAAGACU AAUCGAACCA UCUAGUAGCU GGUUCCUGCC GAAGUUUCCC U CAGGAUAG CAGAAGCUCG UAUCAGUUUU AUGAGGUAAA GCGAAUGAUU AGAGGUUCCG GGGUCGAAAU GACCUUGACC UA UUCUCAA ACUUUAAAUA UGUAAGAAGU CCUUGUUACU UAAUUGAACG UGGACAUUUG AAUGAAGAGC UUUUAGUGGG CCA UUUUUG GUAAGCAGAA CUGGCGAUGC GGGAUGAACC GAACGUAGAG UUAAGGUGCC GGAAUACACG CUCAUCAGAC ACCA CAAAA GGUGUUAGUU CAUCUAGACA GCCGGACGGU GGCCAUGGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACC GGCC GAAUGAACUA GCCCUGAAAA UGGAUGGCGC UCAAGCGUGU UACCUAUACU CUACCGUCAG GGUUGAUAUG AUGCCC UGA CGAGUAGGCA GGCGUGGAGG UCAGUGACGA AGCCUAGACC GUAAGGUCGG GUCGAACGGC CUCUAGUGCA GAUCUUG GU GGUAGUAGCA AAUAUUCAAA UGAGAACUUU GAAGACUGAA GUGGGGAAAG GUUCCACGUC AACAGCAGUU GGACGUGG G UUAGUCGAUC CUAAGAGAUG GGGAAGCUCC GUUUCAAAGG CCUGAUUUUA UGCAGGCCAC CAUCGAAAGG GAAUCCGGU UAAGAUUCCG GAACCUGGAU AUGGAUUCUU CACGGUAACG UAACUGAAUG UGGAGACGUC GGCGCGAGCC CUGGGAGGAG UUAUCUUUU CUUCUUAACA GCUUAUCACC CCGGAAUUGG UUUAUCCGGA GAUGGGGUCU UAUGGCUGGA AGAGGCCAGC A CCUUUGCU GGCUCCGGUG CGCUUGUGAC GGCCCGUGAA AAUCCACAGG AAGGAAUAGU UUUCAUGCCA GGUCGUACUG AU AACCGCA GCAGGUCUCC AAGGUGAACA GCCUCUAGUU GAUAGAAUAA UGUAGAUAAG GGAAGUCGGC AAAAUAGAUC CGU AACUUC GGGAUAAGGA UUGGCUCUAA GGGUCGGGUA GUGAGGGCCU UGGUCAGACG CAGCGGGCGU GCUUGUGGAC UGCU UGGUG GGGCUUGCUC UGCUAGGCGG ACUACUUGCG UGCCUUGUUG UAGACGGCCU UGGUAGGUCU CUUGUAGACC GUCGC UUGC UACAAUUAAC GAUCAACUUA GAACUGGUAC GGACAAGGGG AAUCUGACUG UCUAAUUAAA ACAUAGCAUU GCGAUG GUC AGAAAGUGAU GUUGACGCAA UGUGAUUUCU GCCCAGUGCU CUGAAUGUCA AAGUGAAGAA AUUCAACCAA GCGCGGG UA AACGGCGGGA GUAACUAUGA CUCUCUUAAG GUAGCCAAAU GCCUCGUCAU CUAAUUAGUG ACGCGCAUGA AUGGAUUA A CGAGAUUCCC ACUGUCCCUA UCUACUAUCU AGCGAAACCA CAGCCAAGGG AACGGGCUUG GCAGAAUCAG CGGGGAAAG AAGACCCUGU UGAGCUUGAC UCUAGUUUGA CAUUGUGAAG AGACAUAGAG GGUGUAGAAU AAGUGGGAGC UUCGGCGCCA GUGAAAUAC CACUACCUUU AUAGUUUCUU UACUUAUUCA AUGAAGCGGA GCUGGAAUUC AUUUUCCACG UUCUAGCAUU C AAGGUCCC AUUCGGGGCU GAUCCGGGUU GAAGACAUUG UCAGGUGGGG AGUUUGGCUG GGGCGGCACA UCUGUUAAAC GA UAACGCA GAUGUCCUAA GGGGGGCUCA UGGAGAACAG AAAUCUCCAG UAGAACAAAA GGGUAAAAGC CCCCUUGAUU UUG AUUUUC AGUGUGAAUA CAAACCAUGA AAGUGUGGCC UAUCGAUCCU UUAGUCCCUC GGAAUUUGAG GCUAGAGGUG CCAG AAAAG UUACCACAGG GAUAACUGGC UUGUGGCAGU CAAGCGUUCA UAGCGACAUU GCUUUUUGAU UCUUCGAUGU CGGCU CUUC CUAUCAUACC GAAGCAGAAU UCGGUAAGCG UUGGAUUGUU CACCCACUAA UAGGGAACGU GAGCUGGGUU UAGACC GUC GUGAGACAGG UUAGUUUUAC CCUACUGAUG AAUGUUACCG CAAUAGUAAU UGAACUUAGU ACGAGAGGAA CAGUUCA UU CGGAUAAUUG GUUUUUGCGG CUGUCUGAUC AGGCAUUGCC GCGAAGCUAC CAUCCGCUGG AUUAUGGCUG AACGCCUC U AAGUCAGAAU CCAUGCUAGA ACGCGGUGAU UUCUUUGCUC CACACAAUAU AGAUGGAUAC GAAUAAGGCG UCCUUGUGG CGUCGCUGAA CCAUAGCAGG CUAGCAACGG UGCACUUGGC GGAAAGGCCU UGGGUGCUUG CUGGCGAAUU GCAAUGUCAU UUUGCGUGG GGAUAAAUCA UUUGUAUACG ACUUAGAUGU ACAACGGGGU AUUGUAAGCA GUAGAGUAGC CUUGUUGUUA C GAUCUGCU GAGAUUAAGC CUUUGUUGUC UGAUUUGU

GENBANK: GENBANK: CP011821.1

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分子 #46: ZINC ION

分子名称: ZINC ION / タイプ: ligand / ID: 46 / コピー数: 5 / 式: ZN
分子量理論値: 65.409 Da

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実験情報

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構造解析

手法クライオ電子顕微鏡法
解析単粒子再構成法
試料の集合状態particle

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試料調製

緩衝液pH: 7.5
グリッドモデル: Quantifoil R3/3 / 材質: COPPER / メッシュ: 300 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 3
凍結凍結剤: ETHANE-PROPANE / チャンバー内湿度: 100 %

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電子顕微鏡法

顕微鏡FEI TITAN KRIOS
撮影フィルム・検出器のモデル: FEI FALCON IV (4k x 4k)
平均電子線量: 40.0 e/Å2
電子線加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
電子光学系照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス（公称値）: 0.9 µm / 最小 デフォーカス（公称値）: 0.4 µm
実験機器
モデル: Titan Krios / 画像提供: FEI Company

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画像解析

CTF補正ソフトウェア - 名称: RELION (ver. 4.0.1) / タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
初期モデルモデルのタイプ: PDB ENTRY
PDBモデル - PDB ID:

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詳細: Saccharomyces cerevisiae 60S
最終 再構成解像度のタイプ: BY AUTHOR / 解像度: 3.1 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 4.0.1) / 使用した粒子像数: 14552
初期 角度割当タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 4.0.1)
最終 角度割当タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 4.0.1)

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る