|Entry||Database: EMDB / ID: EMD-4995|
|Title||Subtomogram average of a part of the rabies lyssavirus ribonucleoprotein|
|Biological species||Rabies virus|
|Method||subtomogram averaging / cryo EM / Resolution: 15 Å|
|Authors||Riedel C / Vasishtan D / Prazak V / Ghanem A / Conzelmann K-K / Ruemenapf T|
|Citation||Journal: Sci Rep / Year: 2019|
Title: Cryo EM structure of the rabies virus ribonucleoprotein complex.
Authors: Christiane Riedel / Daven Vasishtan / Vojtěch Pražák / Alexander Ghanem / Karl-Klaus Conzelmann / Till Rümenapf /
Abstract: Rabies virus is an important zoonotic pathogen. Its bullet shaped particle contains a helical nucleocapsid. We used cryo-electron tomography and subsequent subtomogram averaging to determine the ...Rabies virus is an important zoonotic pathogen. Its bullet shaped particle contains a helical nucleocapsid. We used cryo-electron tomography and subsequent subtomogram averaging to determine the structure of its ribonucleoprotein. The resulting electron density map allowed for confident fitting of the N-protein crystal structure, indicating that interactions between neighbouring N-proteins are only mediated by N- and C-terminal protruding subdomains (aa 1-27 and aa 355-372). Additional connecting densities, likely stabilizing the ribonucleoprotein complex, are present between neighbouring M-protein densities on the same helical turn and between M- and N-protein densities located on neighbouring helical turns, but not between M-proteins of different turns, as is observed for the related Vesicular stomatitis virus (VSV). This insight into the architecture of the rabies virus nucleocapsid highlights the surprising structural divergence of large biological assemblies even if the building blocks - here exemplified by VSV M- and N-protein - are structurally closely related.
|Date||Deposition: May 22, 2019 / Header (metadata) release: May 29, 2019 / Map release: Jul 10, 2019 / Update: Jul 17, 2019|
|Structure viewer||EM map: |
Downloads & links
|File||Download / File: emd_4995.map.gz / Format: CCP4 / Size: 844.7 KB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 3.352 Å|
|Symmetry||Space group: 1|
CCP4 map header:
-Entire Rabies virus
|Entire||Name: Rabies virus / Number of components: 1|
-Component #1: virus, Rabies virus
|Virus||Name: Rabies virus / Class: VIRION / Empty: No / Enveloped: Yes / Isolate: STRAIN|
|Species||Species: Rabies virus|
|Source (engineered)||Expression System: Homo sapiens (human) / Cell of expression system: HEK293T|
|Specimen||Specimen state: Particle / Method: cryo EM|
|Sample solution||pH: 7.4|
|Vitrification||Cryogen name: OTHER|
-Electron microscopy imaging
Model: Tecnai Polara / Image courtesy: FEI Company
|Imaging||Microscope: FEI POLARA 300|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 2.5 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: GATAN K2 SUMMIT (4k x 4k)|
|Processing||Method: subtomogram averaging / Applied symmetry: C1 (asymmetric) / Number of subtomograms: 7079|
|3D reconstruction||Resolution: 15 Å / Resolution method: FSC 0.143 CUT-OFF|
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