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- EMDB-49594: Cryo-EM structure of the wild-type Thermus thermophilus 70S ribos... -

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Basic information

Entry
Database: EMDB / ID: EMD-49594
TitleCryo-EM structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys at 2.13A resolution
Map dataFull map of Thermus thermophilus 70S ribosome with 2'deoxy-A76-peptidyl-tRNA and release factor 1
Sample
  • Complex: Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys
    • RNA: x 5 types
    • Protein or peptide: x 51 types
  • Ligand: x 5 types
KeywordsTranslation termination / peptide release / release factor / hydrolysis / peptidyl-tRNA / 70S ribosome / cryo-EM / pre-termination state / peptidyl transferase center / RIBOSOME
Function / homology
Function and homology information


translation release factor activity, codon specific / large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding ...translation release factor activity, codon specific / large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / zinc ion binding / metal ion binding / cytosol / cytoplasm
Similarity search - Function
Peptide chain release factor 1 / : / Peptide chain release factor / PCRF domain / PCRF / Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / Peptide chain release factor class I / RF-1 domain / 30S ribosomal protein Thx ...Peptide chain release factor 1 / : / Peptide chain release factor / PCRF domain / PCRF / Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / Peptide chain release factor class I / RF-1 domain / 30S ribosomal protein Thx / 30S ribosomal protein Thx / 30S ribosomal protein / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / Ribosomal protein S14, type Z / Ribosomal protein L31 type A / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / : / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / : / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / : / Ribosomal protein S19, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L36 / Ribosomal protein S4, bacterial-type / Ribosomal protein L36 superfamily / Ribosomal protein L36 / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S14/S29 / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / : / L28p-like
Similarity search - Domain/homology
Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL19 ...Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS20 / Peptide chain release factor 1 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein bTHX / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL31 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL17
Similarity search - Component
Biological speciesThermus thermophilus (bacteria) / Thermus thermophilus HB8 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.13 Å
AuthorsAleksandrova EV / Syroegin EA / Basu RS / Vassilevski AA / Gagnon MG / Polikanov YS
Funding support United States, 6 items
OrganizationGrant numberCountry
National Science Foundation (NSF, United States)MCB-1907273 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-GM132302 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35-GM151957 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-GM136936 United States
Other governmentUniversity of Illinois Startup Funds
Welch FoundationH-2032-20230405 United States
CitationJournal: Science / Year: 2025
Title: Mechanism of release factor-mediated peptidyl-tRNA hydrolysis on the ribosome
Authors: Aleksandrova EV / Syroegin EA / Basu RS / Vassilevski AA / Gagnon MG / Polikanov YS
History
DepositionMar 8, 2025-
Header (metadata) releaseJun 18, 2025-
Map releaseJun 18, 2025-
UpdateJun 18, 2025-
Current statusJun 18, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_49594.png

Downloads & links

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Map

FileDownload / File: emd_49594.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationFull map of Thermus thermophilus 70S ribosome with 2'deoxy-A76-peptidyl-tRNA and release factor 1
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.84 Å/pix.
x 512 pix.
= 431.258 Å		0.84 Å/pix.
x 512 pix.
= 431.258 Å		0.84 Å/pix.
x 512 pix.
= 431.258 Å

Surface

Projections

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Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.8423 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.02
Minimum - Maximum-0.12407838 - 0.4411776
Average (Standard dev.)0.00015218285 (±0.012346141)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 431.2576 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_49594_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Sharpened map of Thermus thermophilus 70S ribosome with...

Fileemd_49594_additional_1.map
AnnotationSharpened map of Thermus thermophilus 70S ribosome with 2'deoxy-A76-peptidyl-tRNA and release factor 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map of Thermus thermophilus 70S ribosome with...

Fileemd_49594_half_map_1.map
AnnotationHalf map of Thermus thermophilus 70S ribosome with 2'deoxy-A76-peptidyl-tRNA and release factor 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map of Thermus thermophilus 70S ribosome with...

Fileemd_49594_half_map_2.map
AnnotationHalf map of Thermus thermophilus 70S ribosome with 2'deoxy-A76-peptidyl-tRNA and release factor 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q2...

EntireName: Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys
Components
  • Complex: Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys
    • RNA: 23S Ribosomal RNA
    • RNA: 5S Ribosomal RNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L9
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L31
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • RNA: 16S Ribosomal RNA
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14 type Z
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • Protein or peptide: 30S ribosomal protein Thx
    • RNA: CYS-Stop mRNA
    • Protein or peptide: Peptide chain release factor 1
    • RNA: P-site Peptidyl-tRNA fMEAAAKC-tRNAcys RNA-part
    • Protein or peptide: P-site Peptidyl-tRNA fMEAAAKC-tRNAcys Peptide-part
  • Ligand: MAGNESIUM ION
  • Ligand: POTASSIUM ION
  • Ligand: ZINC ION
  • Ligand: IRON/SULFUR CLUSTER
  • Ligand: water

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Supramolecule #1: Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q2...

SupramoleculeName: Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#56
Source (natural)Organism: Thermus thermophilus (bacteria) / Strain: HB8

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Macromolecule #1: 23S Ribosomal RNA

MacromoleculeName: 23S Ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 948.007562 KDa
SequenceString: GUCAAGAUGG UAAGGGCCCA CGGUGGAUGC CUCGGCACCC GAGCCGAUGA AGGACGUGGC UACCUGCGAU AAGCCAGGGG GAGCCGGUA GCGGGCGUGG AUCCCUGGAU GUCCGAAUGG GGGAACCCGG CCGGCGGGAA CGCCGGUCAC CGCGCUUUUG C GCGGGGGG ...String:
GUCAAGAUGG UAAGGGCCCA CGGUGGAUGC CUCGGCACCC GAGCCGAUGA AGGACGUGGC UACCUGCGAU AAGCCAGGGG GAGCCGGUA GCGGGCGUGG AUCCCUGGAU GUCCGAAUGG GGGAACCCGG CCGGCGGGAA CGCCGGUCAC CGCGCUUUUG C GCGGGGGG AACCUGGGGA ACUGAAACAU CUCAGUACCC AGAGGAGAGG AAAGAGAAAU CGACUCCCUG AGUAGCGGCG AG CGAAAGG GGACCAGCCU AAACCGUCCG GCUUGUCCGG GCGGGGUCGU GGGGCCCUCG GACACCGAAU CCCCAGCCUA GCC GAAGCU GUUGGGAAGC AGCGCCAGAG AGGGUGAAAG CCCCGUAGGC GAAAGGUGGG GGGAUAGGUG AGGGUACCCG AGUA CCCCG UGGUUCGUGG AGCCAUGGGG GAAUCUGGGC GGACCACCGC CUAAGGCUAA GUACUCCGGG UGACCGAUAG CGCAC CAGU ACCGUGAGGG AAAGGUGAAA AGAACCCCGG GAGGGGAGUG AAAUAGAGCC UGAAACCGUG GGCUUACAAG CAGUCA CGG CCCCGCAAGG GGUUGUGGCG UGCCUAUUGA AGCAUGAGCC GGCGACUCAC GGUCGUGGGC GAGCUUAAGC CGUUGAG GC GGAGGCGUAG GGAAACCGAG UCCGAACAGG GCGCAAGCGG GCCGCACGCG GCCCGCAAAG UCCGCGGCCG UGGACCCG A AACCGGGCGA GCUAGCCCUG GCCAGGGUGA AGCUGGGGUG AGACCCAGUG GAGGCCCGAA CCGGUGGGGG AUGCAAACC CCUCGGAUGA GCUGGGGCUA GGAGUGAAAA GCUAACCGAG CCCGGAGAUA GCUGGUUCUC CCCGAAAUGA CUUUAGGGUC AGCCUCAGG CGCUGACUGG GGCCUGUAGA GCACUGAUAG GGCUAGGGGG CCCACCAGCC UACCAAACCC UGUCAAACUC C GAAGGGUC CCAGGUGGAG CCUGGGAGUG AGGGCGCGAG CGAUAACGUC CGCGUCCGAG CGCGGGAACA ACCGAGACCG CC AGCUAAG GCCCCCAAGU CUGGGCUAAG UGGUAAAGGA UGUGGCGCCG CGAAGACAGC CAGGAGGUUG GCUUAGAAGC AGC CAUCCU UUAAAGAGUG CGUAAUAGCU CACUGGUCGA GUGGCGCCGC GCCGAAAAUG AUCGGGGCUU AAGCCCAGCG CCGA AGCUG CGGGUCUGGG GGAUGACCCC AGGCGGUAGG GGAGCGUUCC CGAUGCCGAU GAAGGCCGAC CCGCGAGGGC GGCUG GAGG UAAGGGAAGU GCGAAUGCCG GCAUGAGUAA CGAUAAAGAG GGUGAGAAUC CCUCUCGCCG UAAGCCCAAG GGUUCC UAC GCAAUGGUCG UCAGCGUAGG GUUAGGCGGG ACCUAAGGUG AAGCCGAAAG GCGUAGCCGA AGGGCAGCCG GUUAAUA UU CCGGCCCUUC CCGCAGGUGC GAUGGGGGGA CGCUCUAGGC UAGGGGGACC GGAGCCAUGG ACGAGCCCGG CCAGAAGC G CAGGGUGGGA GGUAGGCAAA UCCGCCUCCC AACAAGCUCU GCGUGGUGGG GAAGCCCGUA CGGGUGACAA CCCCCCGAA GCCAGGGAGC CAAGAAAAGC CUCUAAGCAC AACCUGCGGG AACCCGUACC GCAAACCGAC ACAGGUGGGC GGGUGCAAGA GCACUCAGG CGCGCGGGAG AACCCUCGCC AAGGAACUCU GCAAGUUGGC CCCGUAACUU CGGGAGAAGG GGUGCUCCCU G GGGUGAUG AGCCCCGGGG AGCCGCAGUG AACAGGCUCU GGCGACUGUU UACCAAAAAC ACAGCUCUCU GCGAACUCGU AA GAGGAGG UAUAGGGAGC GACGCUUGCC CGGUGCCGGA AGGUCAAGGG GAGGGGUGCA AGCCCCGAAC CGAAGCCCCG GUG AACGGC GGCCG(PSU)AAC(5MU) A(PSU)AA(OMC)GGUCC UAAGGUAGCG AAA(5MU)UC(5MC)UUG UCGGGUAAGU UCCGAC(5MC)UG CACGAAAAGC GUAACGACCG GAGCGCUGUC UCGGCGAGGG ACCCGGUGAA AUUGAACUGG CCGUGAA GA UGCGGCCUAC CCGUGGCAGG ACGAAAAGAC CCCGUGGAGC UUUACUGCAG CCUGGUGUUG GCUCUUGGUC GCGCCUGC G UAGGAUAGGU GGGAGCCUGU GAACCCCCGC CUCCGGGUGG GGGGGAGGCG CCGGUGAAAU ACCACCCUGG CGCGGCUGG GGGCCUAACC CUCGGAUGGG GGGACAGCGC UUGGCGGGCA GUUUGACUG(OMG) GGCGGUCGCC UCCUAAAAGG UAACGG AGG CGCCCAAAGG UCCCCUCAGG CGGGACGGAA AUCCGCCGGA GAGCGCAAGG GUAGAAGGGG GCCUGACUGC GAGGCCU GC AAGCCGAGCA GGGGCGAAAG CCGGGCCUAG UGAACCGGUG GUCCCGUGUG GAAGGGCCAU CGAUCAACGG AUAAAAGU U ACCCCGGGGA UAACAGGCUG AUCUCCCCCG AGCGUCCACA GCGGCGGGGA GGUUUGGCAC CUCG(2MA)UGUCG GCUCG UCGC AUCCUGGGGC UGAAGAAGGU CCCAAGGGUU GGGC(OMU)GUUCG CCCAUUAAAG CGGCACGCGA GCUGGGUUCA GA ACGUCGU GAGACAGU(PSU)C GGUCUCUAUC CGCCACGGGC GCAGGAGGCU UGAGGGGGGC UCUUCCUAGU ACGAGAGGA CCGGAAGGGA CGCACCUCUG GUUUCCCAGC UGUCCCUCCA GGGGCAUAAG CUGGGUAGCC AUGUGCGGAA GGGAUAACCG CUGAAAGCA UCUAAGCGGG AAGCCCGCCC CAAGAUGAGG CCUCCCACGG CGUCAAGCCG GUAAGGACCC GGGAAGACCA C CCGGUGGA UGGGCCGGGG GUGUAAGCGC CGCGAGGCGU UGAGCCGACC GGUCCCAAUC GUCCGAGGUC UUGACCCCUC C

GENBANK: GENBANK: AP008226.1

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Macromolecule #2: 5S Ribosomal RNA

MacromoleculeName: 5S Ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 39.188371 KDa
SequenceString:
UCCCCCGUGC CCAUAGCGGC GUGGAACCAC CCGUUCCCAU UCCGAACACG GAAGUGAAAC GCGCCAGCGC CGAUGGUACU GGGCGGGCG ACCGCCUGGG AGAGUAGGUC GGUGCGGGGG AU

GENBANK: GENBANK: AP008226.1

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Macromolecule #32: 16S Ribosomal RNA

MacromoleculeName: 16S Ribosomal RNA / type: rna / ID: 32 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 493.863625 KDa
SequenceString: UUGUUGGAGA GUUUGAUCCU GGCUCAGGGU GAACGCUGGC GGCGUGCCUA AGACAUGCAA GUCGUGCGGG CCGCGGGGUU UUACUCCGU GGUCAGCGGC GGACGGGUGA GUAACGCGUG GGUGACCUAC CCGGAAGAGG GGGACAACCC GGGGAAACUC G GGCUAAUC ...String:
UUGUUGGAGA GUUUGAUCCU GGCUCAGGGU GAACGCUGGC GGCGUGCCUA AGACAUGCAA GUCGUGCGGG CCGCGGGGUU UUACUCCGU GGUCAGCGGC GGACGGGUGA GUAACGCGUG GGUGACCUAC CCGGAAGAGG GGGACAACCC GGGGAAACUC G GGCUAAUC CCCCAUGUGG ACCCGCCCCU UGGGGUGUGU CCAAAGGGCU UUGCCCGCUU CCGGAUGGGC CCGCGUCCCA UC AGCUAGU UGGUGGGGUA AUGGCCCACC AAGGCGACGA CGGGUAGCCG GUCUGAGAGG AUGGCCGGCC ACAGGGGCAC UGA GACACG GGCCCCACUC CUACGGGAGG CAGCAGUUAG GAAUCUUCCG CAAUGGGCGC AAGCCUGACG GAGCGACGCC GCUU GGAGG AAGAAGCCCU UCGGGGUGUA AACUCCUGAA CCCGGGACGA AACCCCCGAC GAGGGGACUG ACGGUACCGG GGUAA UAGC GCCGGCCAAC UCCG(PSU)GCCAG CAGCC(G7M)CGGU AAUACGGAGG GCGCGAGCGU UACCCGGAUU CACUGGGC G UAAAGGGCGU GUAGGCGGCC UGGGGCGUCC CAUGUGAAAG ACCACGGCUC AACCGUGGGG GAGCGUGGGA UACGCUCAG GCUAGACGGU GGGAGAGGGU GGUGGAAUUC CCGGAGUAGC GGUGAAAUGC GCAGAUACCG GGAGGAACGC CGAUGGCGAA GGCAGCCAC CUGGUCCACC CGUGACGCUG AGGCGCGAAA GCGUGGGGAG CAAACCGGAU UAGAUACCCG GGUAGUCCAC G CCCUAAAC GAUGCGCGCU AGGUCUCUGG GUCUCCUGGG GGCCGAAGCU AACGCGUUAA GCGCGCCGCC UGGGGAGUAC GG CCGCAAG GCUGAAACUC AAAGGAAUUG ACGGGGGCCC GCACAAGCGG UGGAGCAUGU GGUUUAAUUC GAA(M2G)(5MC) AACG CGAAGAACCU UACCAGGCCU UGACAUGCUA GGGAACCCGG GUGAAAGCCU GGGGUGCCCC GCGAGGGGAG CCCUAG CAC AGGUGCUGCA UGGCCGUCGU CAGCUCGUGC CGUGAGGUGU UGGGUUAAGU CCCGCAACGA GCGCAACCCC CGCCGUU AG UUGCCAGCGG UUCGGCCGGG CACUCUAACG GGACUGCCCG CGAAAGCGGG AGGAAGGAGG GGACGACGUC UGGUCAGC A UG(2MG)CCCUUAC GGCCUGGGCG ACACACGUGC UACAAUGCCC ACUACAAAGC GAUGCCACCC GGCAACGGGG AGCUA AUCG CAAAAAGGUG GGCCCAGUUC GGAUUGGGGU CUGCAACCCG ACCCCAUGAA GCCGGAAUCG CUAGUAAUCG CGGAUC AGC CAUGCCGCGG UGAAUACGUU CCCGGGCCUU GUACACAC(5MC)G (4OC)C(5MC)GU(5MC)ACGC CAUGGGAGCG G GCUCUACC CGAAGUCGCC GGGAGCCUAC GGGCAGGCGC CGAGGGUAGG GCCCGUGACU GGGGCGAAGU CG(UR3)AACAA G GUAGCUGUAC CGG(MA6)(MA6)GGUGC GGCUGGAUCA CCUCCUUUCU

GENBANK: GENBANK: AP008226.1

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Macromolecule #53: CYS-Stop mRNA

MacromoleculeName: CYS-Stop mRNA / type: rna / ID: 53 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 7.827775 KDa
SequenceString:
GGCAAGGAGG UAAAAUGCUA AUAA

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Macromolecule #55: P-site Peptidyl-tRNA fMEAAAKC-tRNAcys RNA-part

MacromoleculeName: P-site Peptidyl-tRNA fMEAAAKC-tRNAcys RNA-part / type: rna / ID: 55 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 23.927459 KDa
SequenceString:
GGCGCGU(4SU)AA CAAAGCGG(H2U)(H2U) AUGUAGCGGA (PSU)UGCA(MIA)A(PSU)CC GUCUAGUCCG G(5MU) (PSU)CGACUC CGGAACGCGC CUCCA

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Macromolecule #3: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 30.532551 KDa
SequenceString: MAVKKFKPYT PSRRFMTVAD FSEITKTEPE KSLVKPLKKT GGRNNQGRIT VRFRGGGHKR LYRIIDFKRW DKVGIPAKVA AIEYDPNRS ARIALLHYVD GEKRYIIAPD GLQVGQQVVA GPDAPIQVGN ALPLRFIPVG TVVHAVELEP KKGAKLARAA G TSAQIQGR ...String:
MAVKKFKPYT PSRRFMTVAD FSEITKTEPE KSLVKPLKKT GGRNNQGRIT VRFRGGGHKR LYRIIDFKRW DKVGIPAKVA AIEYDPNRS ARIALLHYVD GEKRYIIAPD GLQVGQQVVA GPDAPIQVGN ALPLRFIPVG TVVHAVELEP KKGAKLARAA G TSAQIQGR EGDYVILRLP SGELRKVHGE CYATVGAVGN ADHKNIVLGK AGRSRWLGRR PHVRGAAMNP VDHPHGGGEG RA PRGRPPA SPWGWQTKGL KTRKRRKPSS RFIIARRKK

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #4: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 22.450213 KDa
SequenceString: MKGILGVKVG MTRIFRDDRA VPVTVILAGP CPVVQRRTPE KDGYTAVQLG FLPQNPKRVN RPLKGHFAKA GVEPVRILRE IRDFNPEGD TVTVEIFKPG ERVDVTGTSK GRGFAGVMKR WNFAGGPDSH GAHKIHRHPG SIGNRKTPGR VYKGKKMAGH Y GAERVTVM ...String:
MKGILGVKVG MTRIFRDDRA VPVTVILAGP CPVVQRRTPE KDGYTAVQLG FLPQNPKRVN RPLKGHFAKA GVEPVRILRE IRDFNPEGD TVTVEIFKPG ERVDVTGTSK GRGFAGVMKR WNFAGGPDSH GAHKIHRHPG SIGNRKTPGR VYKGKKMAGH Y GAERVTVM NLEVVDVIPE ENLLLVKGAV PGPNGGLVIV RETKKAAK

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #5: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 23.271914 KDa
SequenceString: MKEVAVYQIP VLSPSGRREL AADLPAEINP HLLWEVVRWQ LAKRRRGTAS TKTRGEVAYS GRKIWPQKHT GRARHGDIGA PIFVGGGVV FGPKPRDYSY TLPKKVRKKG LAMAVADRAR EGKLLLVEAF AGVNGKTKEF LAWAKEAGLD GSESVLLVTG N ELVRRAAR ...String:
MKEVAVYQIP VLSPSGRREL AADLPAEINP HLLWEVVRWQ LAKRRRGTAS TKTRGEVAYS GRKIWPQKHT GRARHGDIGA PIFVGGGVV FGPKPRDYSY TLPKKVRKKG LAMAVADRAR EGKLLLVEAF AGVNGKTKEF LAWAKEAGLD GSESVLLVTG N ELVRRAAR NLPWVVTLAP EGLNVYDIVR TERLVMDLDA WEVFQNRIGG EA

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #6: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 21.061596 KDa
SequenceString:
MPLDVALKRK YYEEVRPELI RRFGYQNVWE VPRLEKVVIN QGLGEAKEDA RILEKAAQEL ALITGQKPAV TRAKKSISNF KLRKGMPIG LRVTLRRDRM WIFLEKLLNV ALPRIRDFRG LNPNSFDGRG NYNLGLREQL IFPEITYDMV DALRGMDIAV V TTAETDEE ARALLELLGF PFRK

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #7: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 19.568926 KDa
SequenceString:
MSRIGRLPIP VPKGVSVEVA PGRVKVKGPK GELEVPVSPE MRVVVEEGVV RVERPSDERR HKSLHGLTRT LIANAVKGVS EGYSKELLI KGIGYRARLV GRALELTVGF SHPVVVEPPE GITFEVPEPT RVRVSGIDKQ KVGQVAANIR AIRKPSAYHE K GIYYAGEP VRLKPGKAGA KK

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #8: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 16.42218 KDa
SequenceString:
MKVILLEPLE NLGDVGQVVD VKPGYARNYL LPRGLAVLAT ESNLKALEAR IRAQAKRLAE RKAEAERLKE ILENLTLTIP VRAGETKIY GSVTAKDIAE ALSRQHGVTI DPKRLALEKP IKELGEYVLT YKPHPEVPIQ LKVSVVAQE

UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #9: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 15.927903 KDa
SequenceString:
MKTYVPKQVE PRWVLIDAEG KTLGRLATKI ATLLRGKHRP DWTPNVAMGD FVVVVNADKI RVTGKKLEQK IYTRYSGYPG GLKKIPLEK MLATHPERVL EHAVKGMLPK GPLGRRLFKR LKVYAGPDHP HQAQRPEKLE V

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #10: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 13.323612 KDa
SequenceString:
MIQPQTYLEV ADNTGARKIM CIRVLKGSNA KYATVGDVIV ASVKEAIPRG AVKEGDVVKA VVVRTKKEIK RPDGSAIRFD DNAAVIINN QLEPRGTRVF GPVARELREK GFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #11: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 16.319142 KDa
SequenceString:
MKLSDLRPNP GANKRRKRVG RGPGSGHGKT ATRGHKGQKS RSGGLKDPRR FEGGRSTTLM RLPKRGMQGQ VPGEIKRPRY QGVNLKDLA RFEGEVTPEL LVRAGLLKKG YRLKILGEGE AKPLKVVAHA FSKSALEKLK AAGGEPVLLE A

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #12: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 15.993909 KDa
SequenceString:
MLMPRRMKYR KQQRGRLKGA TKGGDYVAFG DYGLVALEPA WITAQQIEAA RVAMVRHFRR GGKIFIRIFP DKPYTKKPLE VRMGKGKGN VEGYVAVVKP GRVMFEVAGV TEEQAMEALR IAGHKLPIKT KIVRRDAYDE AQ

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #13: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 13.750148 KDa
SequenceString:
MRHLKSGRKL NRHSSHRLAL YRNQAKSLLT HGRITTTVPK AKELRGFVDH LIHLAKRGDL HARRLVLRDL QDVKLVRKLF DEIAPRYRD RQGGYTRVLK LAERRRGDGA PLALVELVE

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #14: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 12.639845 KDa
SequenceString:
MARLTAYERR KFRVRNRIKR TGRLRLSVFR SLKHIYAQII DDEKGVTLVS ASSLALKLKG NKTEVARQVG RALAEKALAL GIKQVAFDR GPYKYHGRVK ALAEGAREGG LEF

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #15: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 17.18883 KDa
SequenceString:
MNRGALIKLV ESRYVRTDLP EFRPGDTVRV SYKVKEGNRT RIQDFEGIVI RIRRNGFNTT FTVRKVSYGV GVERIFPLHS PLIQKIDIV QRGRARRAKL YFIRNLSDRE IRRKLRADRK RIDQDRAAER AAKEEAQKAQ EPKASQE

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #16: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 13.779275 KDa
SequenceString:
MPRAKTGVVR RRKHKKILKL AKGYWGLRSK SFRKARETLF AAGNYAYAHR KRRKRDFRRL WIVRINAACR QHGLNYSTFI HGLKKAGIE VDRKNLADLA VREPQVFAEL VERAKAAQG

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #17: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 11.069153 KDa
SequenceString:
MFAIVKTGGK QYRVEPGLKL RVEKLDAEPG ATVELPVLLL GGEKTVVGTP VVEGASVVAE VLGHGRGKKI LVSKFKAKVQ YRRKKGHRQ PYTELLIKEI RG

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #18: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 12.808055 KDa
SequenceString:
MEAKAIARYV RISPRKVRLV VDLIRGKSLE EARNILRYTN KRGAYFVAKV LESAAANAVN NHDMLEDRLY VKAAYVDEGP ALKRVLPRA RGRADIIKKR TSHITVILGE KHGK

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #19: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 10.759808 KDa
SequenceString:
MKTAYDVILA PVLSEKAYAG FAEGKYTFWV HPKATKTEIK NAVETAFKVK VVKVNTLHVR GKKKRLGRYL GKRPDRKKAI VQVAPGQKI EALEGLI

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #20: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 12.085611 KDa
SequenceString:
MRVKMHVKKG DTVLVASGKY KGRVGKVKEV LPKKYAVIVE GVNIVKKAVR VSPKYPQGGF IEKEAPLHAS KVRPICPACG KPTRVRKKF LENGKKIRVC AKCGGALDTE E

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #21: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 23.238572 KDa
SequenceString: MEYRLKAYYR EGEKPSALRR AGKLPGVMYN RHLNRKVYVD LVEFDKVFRQ ASIHHVIVLE LPDGQSLPTL VRQVNLDKRR RRPEHVDFF VLSDEPVEMY VPLRFVGTPA GVRAGGVLQE IHRDILVKVS PRNIPEFIEV DVSGLEIGDS LHASDLKLPP G VELAVSPE ...String:
MEYRLKAYYR EGEKPSALRR AGKLPGVMYN RHLNRKVYVD LVEFDKVFRQ ASIHHVIVLE LPDGQSLPTL VRQVNLDKRR RRPEHVDFF VLSDEPVEMY VPLRFVGTPA GVRAGGVLQE IHRDILVKVS PRNIPEFIEV DVSGLEIGDS LHASDLKLPP G VELAVSPE ETIAAVVPPE DVEKLAEEAA AEVAEPEVIK KGKEEEEE

UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #22: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 9.529074 KDa
SequenceString:
MAHKKGLGST RNGRDSQAKR LGVKRYEGQV VRAGNILVRQ RGTRFKPGKN VGMGRDFTLF ALVDGVVEFQ DRGRLGRYVH VRPLA

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #23: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 11.00416 KDa
SequenceString:
MSKVCEISGK RPIVANSIQR RGKAKREGGV GKKTTGISKR RQYPNLQKVR VRVAGQEITF RVAASHIPKV YELVERAKGL KLEGLSPKE IKKELLKLL

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #24: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 8.670258 KDa
SequenceString:
MKLSEVRKQL EEARKLSPVE LEKLVREKKR ELMELRFQAS IGQLSQNHKI RDLKRQIARL LTVLNEKRRQ NA

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #25: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 6.799126 KDa
SequenceString:
MPRLKVKLVK SPIGYPKDQK AALKALGLRR LQQERVLEDT PAIRGNVEKV AHLVRVEVVE

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #26: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 8.300543 KDa
SequenceString:
MKEGIHPKLV PARIICGCGN VIETYSTKPE IYVEVCSKCH PFYTGQQRFV DTEGRVERFQ RRYGDSYRKG R

UniProtKB: Large ribosomal subunit protein bL31

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Macromolecule #27: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 6.72205 KDa
SequenceString:
MAKHPVPKKK TSKARRDARR SHHALTPPTL VPCPECKAMK PPHTVCPECG YYAGRKVLEV

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #28: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 6.632896 KDa
SequenceString:
MASEVRIKLL LECTECKRRN YATEKNKRNT PNKLELRKYC PWCRKHTVHR EVKI

UniProtKB: Large ribosomal subunit protein bL33

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Macromolecule #29: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 6.132449 KDa
SequenceString:
MKRTWQPNRR KRAKTHGFRA RMRTPGGRKV LKRRRQKGRW RLTPAVRKR

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #30: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 7.506178 KDa
SequenceString:
MPKMKTHKGA KKRVKITASG KVVAMKTGKR HLNWQKSGKE IRQKGRKFVL AKPEAERIKL LLPYE

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #31: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 4.435411 KDa
SequenceString:
MKVRASVKRI CDKCKVIRRH GRVYVICENP KHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #33: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 29.317703 KDa
SequenceString: MPVEITVKEL LEAGVHFGHE RKRWNPKFAR YIYAERNGIH IIDLQKTMEE LERTFRFIED LAMRGGTILF VGTKKQAQDI VRMEAERAG MPYVNQRWLG GMLTNFKTIS QRVHRLEELE ALFASPEIEE RPKKEQVRLK HELERLQKYL SGFRLLKRLP D AIFVVDPT ...String:
MPVEITVKEL LEAGVHFGHE RKRWNPKFAR YIYAERNGIH IIDLQKTMEE LERTFRFIED LAMRGGTILF VGTKKQAQDI VRMEAERAG MPYVNQRWLG GMLTNFKTIS QRVHRLEELE ALFASPEIEE RPKKEQVRLK HELERLQKYL SGFRLLKRLP D AIFVVDPT KEAIAVREAR KLFIPVIALA DTDSDPDLVD YIIPGNDDAI RSIQLILSRA VDLIIQARGG VVEPSPSYAL VQ EAEATET PEGESEVEA

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #34: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 26.751076 KDa
SequenceString: MGNKIHPIGF RLGITRDWES RWYAGKKQYR HLLLEDQRIR GLLEKELYSA GLARVDIERA ADNVAVTVHV AKPGVVIGRG GERIRVLRE ELAKLTGKNV ALNVQEVQNP NLSAPLVAQR VAEQIERRFA VRRAIKQAVQ RVMESGAKGA KVIVSGRIGG A EQARTEWA ...String:
MGNKIHPIGF RLGITRDWES RWYAGKKQYR HLLLEDQRIR GLLEKELYSA GLARVDIERA ADNVAVTVHV AKPGVVIGRG GERIRVLRE ELAKLTGKNV ALNVQEVQNP NLSAPLVAQR VAEQIERRFA VRRAIKQAVQ RVMESGAKGA KVIVSGRIGG A EQARTEWA AQGRVPLHTL RANIDYGFAL ARTTYGVLGV KAYIFLGEVI GGQKPKARPE LPKAEERPRR RRPAVRVKKE E

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #35: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 24.373447 KDa
SequenceString: MGRYIGPVCR LCRREGVKLY LKGERCYSPK CAMERRPYPP GQHGQKRARR PSDYAVRLRE KQKLRRIYGI SERQFRNLFE EASKKKGVT GSVFLGLLES RLDNVVYRLG FAVSRRQARQ LVRHGHITVN GRRVDLPSYR VRPGDEIAVA EKSRNLELIR Q NLEAMKGR ...String:
MGRYIGPVCR LCRREGVKLY LKGERCYSPK CAMERRPYPP GQHGQKRARR PSDYAVRLRE KQKLRRIYGI SERQFRNLFE EASKKKGVT GSVFLGLLES RLDNVVYRLG FAVSRRQARQ LVRHGHITVN GRRVDLPSYR VRPGDEIAVA EKSRNLELIR Q NLEAMKGR KVGPWLSLDV EGMKGKFLRL PDREDLALPV NEQLVIEFYS R

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #36: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 17.583416 KDa
SequenceString:
MPETDFEEKM ILIRRTARMQ AGGRRFRFGA LVVVGDRQGR VGLGFGKAPE VPLAVQKAGY YARRNMVEVP LQNGTIPHEI EVEFGASKI VLKPAAPGTG VIAGAVPRAI LELAGVTDIL TKELGSRNPI NIAYATMEAL RQLRTKADVE RLRKGEAHAQ A QG

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #37: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 11.988753 KDa
SequenceString:
MRRYEVNIVL NPNLDQSQLA LEKEIIQRAL ENYGARVEKV EELGLRRLAY PIAKDPQGYF LWYQVEMPED RVNDLARELR IRDNVRRVM VVKSQEPFLA NA

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #38: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 18.050973 KDa
SequenceString:
MARRRRAEVR QLQPDLVYGD VLVTAFINKI MRDGKKNLAA RIFYDACKII QEKTGQEPLK VFKQAVENVK PRMEVRSRRV GGANYQVPM EVSPRRQQSL ALRWLVQAAN QRPERRAAVR IAHELMDAAE GKGGAVKKKE DVERMAEANR AYAHYRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #39: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 15.86857 KDa
SequenceString:
MLTDPIADML TRIRNATRVY KESTDVPASR FKEEILRILA REGFIKGYER VDVDGKPYLR VYLKYGPRRQ GPDPRPEQVI HHIRRISKP GRRVYVGVKE IPRVRRGLGI AILSTSKGVL TDREARKLGV GGELICEVW

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #40: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 14.410614 KDa
SequenceString:
MEQYYGTGRR KEAVARVFLR PGNGKVTVNG QDFNEYFQGL VRAVAALEPL RAVDALGHFD AYITVRGGGK SGQIDAIKLG IARALVQYN PDYRAKLKPL GFLTRDARVV ERKKYGKHKA RRAPQYSKR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #41: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 11.954968 KDa
SequenceString:
MPKIRIKLRG FDHKTLDASA QKIVEAARRS GAQVSGPIPL PTRVRRFTVI RGPFKHKDSR EHFELRTHNR LVDIINPNRK TIEQLMTLD LPTGVEIEIK TVGGGR

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #42: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 13.737868 KDa
SequenceString:
MAKKPSKKKV KRQVASGRAY IHASYNNTIV TITDPDGNPI TWSSGGVIGY KGSRKGTPYA AQLAALDAAK KAMAYGMQSV DVIVRGTGA GREQAIRALQ ASGLQVKSIV DDTPVPHNGC RPKKKFRKAS

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #43: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 14.683476 KDa
SequenceString:
MPTINQLVRK GREKVRKKSK VPALKGAPFR RGVCTVVRTV TPKKPNSALR KVAKVRLTSG YEVTAYIPGE GHNLQEHSVV LIRGGRVK(0TD) LPGVRYHIVR GVYDAAGVKD RKKSRSKYGT KKPKEAAKTA AKK

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #44: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 14.338861 KDa
SequenceString:
MARIAGVEIP RNKRVDVALT YIYGIGKARA KEALEKTGIN PATRVKDLTE AEVVRLREYV ENTWKLEGEL RAEVAANIKR LMDIGCYRG LRHRRGLPVR GQRTRTNART RKGPRKTVAG KKKAPRK

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #45: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 7.158725 KDa
SequenceString:
MARKALIEKA KRTPKFKVRA YTRCVRCGRA RSVYRFFGLC RICLRELAHK GQLPGVRKAS W

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #46: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 10.578407 KDa
SequenceString:
MPITKEEKQK VIQEFARFPG DTGSTEVQVA LLTLRINRLS EHLKVHKKDH HSHRGLLMMV GQRRRLLRYL QREDPERYRA LIEKLGIRG

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #47: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 10.409983 KDa
SequenceString:
MVKIRLARFG SKHNPHYRIV VTDARRKRDG KYIEKIGYYD PRKTTPDWLK VDVERARYWL SVGAQPTDTA RRLLRQAGVF RQEAREGA

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #48: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 12.325655 KDa
SequenceString:
MPKKVLTGVV VSDKMQKTVT VLVERQFPHP LYGKVIKRSK KYLAHDPEEK YKLGDVVEII ESRPISKRKR FRVLRLVESG RMDLVEKYL IRRQNYESLS KRGGKA

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #49: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 10.258299 KDa
SequenceString:
MSTKNAKPKK EAQRRPSRKA KVKATLGEFD LRDYRNVEVL KRFLSETGKI LPRRRTGLSA KEQRILAKTI KRARILGLLP FTEKLVRK

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #50: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 10.605464 KDa
SequenceString:
MPRSLKKGVF VDDHLLEKVL ELNAKGEKRL IKTWSRRSTI VPEMVGHTIA VYNGKQHVPV YITENMVGHK LGEFAPTRTY RGHGKEAKA TKKK

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #51: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 11.736143 KDa
SequenceString:
MAQKKPKRNL SALKRHRQSL KRRLRNKAKK SAIKTLSKKA IQLAQEGKAE EALKIMRKAE SLIDKAAKGS TLHKNAAARR KSRLMRKVR QLLEAAGAPL IGGGLSA

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #52: 30S ribosomal protein Thx

MacromoleculeName: 30S ribosomal protein Thx / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 3.35003 KDa
SequenceString:
MGKGDRRTRR GKIWRGTYGK YRPRKKK

UniProtKB: Small ribosomal subunit protein bTHX

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Macromolecule #54: Peptide chain release factor 1

MacromoleculeName: Peptide chain release factor 1 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 40.167559 KDa
SequenceString: MLDKLDRLEE EYRELEALLS DPEVLKDKGR YQSLSRRYAE MGEVIGLIRE YRKVLEDLEQ AESLLDDPEL KEMAKAEREA LLARKEALE KELERHLLPK DPMDERDAIV EIRAGTGGEE AALFARDLFN MYLRFAEEMG FETEVLDSHP TDLGGFSKVV F EVRGPGAY ...String:
MLDKLDRLEE EYRELEALLS DPEVLKDKGR YQSLSRRYAE MGEVIGLIRE YRKVLEDLEQ AESLLDDPEL KEMAKAEREA LLARKEALE KELERHLLPK DPMDERDAIV EIRAGTGGEE AALFARDLFN MYLRFAEEMG FETEVLDSHP TDLGGFSKVV F EVRGPGAY GTFKYESGVH RVQRVPVTET QGRIHTSTAT VAVLPKAEEE DFALNMDEIR IDVMRASGPG G(MEQ)GVNTTD S AVRVVHLPTG IMVTCQDSRS QIKNREKALM ILRSRLLEMK RAEEAERLRK TRLAQIGTGE RSEKIRTYNF PQSRVTDHR IGFTTHDLEG VLSGHLTPIL EALKRADQER QLAALAEG

UniProtKB: Peptide chain release factor 1

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Macromolecule #56: P-site Peptidyl-tRNA fMEAAAKC-tRNAcys Peptide-part

MacromoleculeName: P-site Peptidyl-tRNA fMEAAAKC-tRNAcys Peptide-part / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 751.891 Da
SequenceString:
(FME)EAAAKC

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Macromolecule #57: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 57 / Number of copies: 1685 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #58: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 58 / Number of copies: 52 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #59: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 59 / Number of copies: 6 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #60: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 60 / Number of copies: 1 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Macromolecule #61: water

MacromoleculeName: water / type: ligand / ID: 61 / Number of copies: 4759 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.375 mg/mL
BufferpH: 7.6
Details: 5 mM HEPES-KOH, 50 mM KCl, 10 mM NH4Cl, 10 mM Mg(CH3COO)2
GridModel: Quantifoil R2/1 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec. / Pretreatment - Pressure: 0.024 kPa
VitrificationCryogen name: ETHANE / Chamber humidity: 85 % / Chamber temperature: 295 K / Instrument: LEICA EM GP

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Electron microscopy

MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Detector mode: COUNTING / Number grids imaged: 1 / Number real images: 25104 / Average exposure time: 1.1984 sec. / Average electron dose: 39.7153 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 105000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC (ver. 4.6.1) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.13 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.6.1) / Number images used: 617938
Initial angle assignmentType: RANDOM ASSIGNMENT / Software - Name: cryoSPARC (ver. 4.6.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.6.1)
Final 3D classificationSoftware - Name: cryoSPARC (ver. 4.6.1)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

9mtp


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-9no7:
Cryo-EM structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys at 2.13A resolution

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