postsynaptic cytoskeleton / Acetylcholine regulates insulin secretion / epithelial cell maturation involved in salivary gland development / positive regulation of vascular wound healing / positive regulation of protein acetylation / ATF6-mediated unfolded protein response / outer dense fiber / organelle / intracellular transport of virus / negative regulation of striated muscle cell apoptotic process ...postsynaptic cytoskeleton / Acetylcholine regulates insulin secretion / epithelial cell maturation involved in salivary gland development / positive regulation of vascular wound healing / positive regulation of protein acetylation / ATF6-mediated unfolded protein response / outer dense fiber / organelle / intracellular transport of virus / negative regulation of striated muscle cell apoptotic process / regulation of skeletal muscle fiber development / glandular epithelial cell maturation / N-terminal peptidyl-glycine N-myristoylation / peptidyl-lysine N6-myristoyltransferase activity / sterol homeostasis / positive regulation of cell proliferation involved in heart morphogenesis / IRE1alpha activates chaperones / positive regulation of skeletal muscle tissue growth / ATF6 (ATF6-alpha) activates chaperone genes / positive regulation of plasma cell differentiation / positive regulation of phospholipid biosynthetic process / positive regulation of ERAD pathway / regulation of opsin-mediated signaling pathway / negative regulation of myotube differentiation / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / intracellular triglyceride homeostasis / cardiac ventricle development / ventricular cardiac muscle tissue development / actin crosslink formation / cellular response to fructose stimulus / positive regulation of lactation / chromatoid body / glycylpeptide N-tetradecanoyltransferase / glycylpeptide N-tetradecanoyltransferase activity / cellular response to laminar fluid shear stress / XBP1(S) activates chaperone genes / regulation of modification of postsynaptic actin cytoskeleton / cellular response to nutrient / heart trabecula morphogenesis / positive regulation of hepatocyte proliferation / positive regulation of dendritic spine morphogenesis / bleb / skeletal muscle tissue regeneration / dendritic branch / cellular response to fluid shear stress / positive regulation of MHC class II biosynthetic process / exocrine pancreas development / germinal vesicle / endothelial cell proliferation / positive regulation of vascular associated smooth muscle cell migration / positive regulation of cytokine production involved in inflammatory response / negative regulation of endoplasmic reticulum unfolded protein response / protein localization to membrane / neural tube development / cellular response to peptide hormone stimulus / positive regulation of B cell differentiation / positive regulation of T cell differentiation / muscle organ development / positive regulation of immunoglobulin production / phosphatidylserine binding / IRE1-mediated unfolded protein response / cell-substrate adhesion / negative regulation of SMAD protein signal transduction / actin filament bundle / ubiquitin-like protein ligase binding / actin filament bundle assembly / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / cellular response to vascular endothelial growth factor stimulus / positive regulation of endothelial cell apoptotic process / positive regulation of TOR signaling / adipose tissue development / fatty acid homeostasis / vascular endothelial growth factor receptor signaling pathway / positive regulation of insulin receptor signaling pathway / ubiquitin ligase inhibitor activity / 90S preribosome / positive regulation of fat cell differentiation / positive regulation of signal transduction by p53 class mediator / response to insulin-like growth factor stimulus / neuron development / negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway / cellular response to glucose starvation / cis-regulatory region sequence-specific DNA binding / phagocytic cup / positive regulation of vascular associated smooth muscle cell proliferation / presynaptic cytosol / endoplasmic reticulum unfolded protein response / eNOS activation / ERAD pathway / translation regulator activity / rough endoplasmic reticulum / ribosomal small subunit export from nucleus / gastrulation / MDM2/MDM4 family protein binding / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / Membrane binding and targetting of GAG proteins / cytosolic ribosome / neurogenesis / positive regulation of autophagy Similarity search - Function
Myristoylated alanine-rich C-kinase substrate MARCKS / MARCKS family / MARCKS family signature 1. / MARCKS family phosphorylation site domain. / : / Nascent polypeptide-associated complex subunit alpha / Nascent polypeptide-associated complex subunit alpha-like, UBA domain / HYPK UBA domain / Transcription factor BTF3 / Nascent polypeptide-associated complex NAC domain ...Myristoylated alanine-rich C-kinase substrate MARCKS / MARCKS family / MARCKS family signature 1. / MARCKS family phosphorylation site domain. / : / Nascent polypeptide-associated complex subunit alpha / Nascent polypeptide-associated complex subunit alpha-like, UBA domain / HYPK UBA domain / Transcription factor BTF3 / Nascent polypeptide-associated complex NAC domain / NAC A/B domain superfamily / NAC domain / NAC A/B domain profile. / NAC / Glycylpeptide N-tetradecanoyltransferase, conserved site / Myristoyl-CoA:protein N-myristoyltransferase signature 1. / Myristoyl-CoA:protein N-myristoyltransferase signature 2. / Glycylpeptide N-tetradecanoyltransferase / Glycylpeptide N-tetradecanoyltransferase, N-terminal / Glycylpeptide N-tetradecanoyltransferase, C-terminal / Myristoyl-CoA:protein N-myristoyltransferase, N-terminal domain / Myristoyl-CoA:protein N-myristoyltransferase, C-terminal domain / Basic region leucine zipper / Basic-leucine zipper (bZIP) domain signature. / Basic-leucine zipper (bZIP) domain profile. / basic region leucin zipper / Basic-leucine zipper domain / Basic-leucine zipper domain superfamily / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ubiquitin-like protein FUBI / 50S ribosomal protein L10, insertion domain superfamily / Ribosomal protein L30e / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / : / Ribosomal protein L23 / Ribosomal protein L2, archaeal-type / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / metallochaperone-like domain / TRASH domain / Ribosomal protein L41 / Ribosomal protein L41 / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein L29e / Ribosomal L29e protein family / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / S27a-like superfamily / Ribosomal protein L10e, conserved site / : / Ribosomal protein L10e signature. / Ribosomal protein L6e signature. / Ribosomal protein L44e signature. / 40S Ribosomal protein S10 / Ribosomal protein L10e / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein S30 / Ribosomal protein S30 / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / : / : / Ribosomal protein S27a / Ribosomal L40e family / Ribosomal protein S27a / Ribosomal protein S7e signature. / Ribosomal protein S27a / Ribosomal protein L30e signature 1. / Ribosomal protein L36e signature. / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Eukaryotic Ribosomal Protein L27, KOW domain Similarity search - Domain/homology
Small ribosomal subunit protein eS32 / Small ribosomal subunit protein eS24 / Ribosomal protein L36a / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS5 / 60S ribosomal protein L32 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL24 ...Small ribosomal subunit protein eS32 / Small ribosomal subunit protein eS24 / Ribosomal protein L36a / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS5 / 60S ribosomal protein L32 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL29 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein eS1 / Large ribosomal subunit protein eL8 / Small ribosomal subunit protein eS7 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein eL14 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein eL15 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein uL14 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS25 / Large ribosomal subunit protein eL30 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein eS28 / Small ribosomal subunit protein eS4 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL36 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL34 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL28 / Small ribosomal subunit protein uS14 / Glycylpeptide N-tetradecanoyltransferase 2 / Ubiquitin-ribosomal protein eL40 fusion protein / X-box-binding protein 1 / Large ribosomal subunit protein uL18 / Transcription factor BTF3 / Myristoylated alanine-rich C-kinase substrate / Nascent polypeptide-associated complex subunit alpha / Large ribosomal subunit protein eL37 Similarity search - Component
Biological species
Oryctolagus cuniculus (rabbit) / Homo sapiens (human)
Method
single particle reconstruction / cryo EM / Resolution: 2.82 Å
Journal: EMBO J / Year: 2025 Title: NAC couples protein synthesis with nascent polypeptide myristoylation on the ribosome. Authors: Sara Zdancewicz / Emir Maldosevic / Kinga Malezyna / Ahmad Jomaa / Abstract: N-glycine myristoylation allows for reversible association of newly synthesized proteins with membranes to regulate essential functions such as cellular signaling and stress responses. This process ...N-glycine myristoylation allows for reversible association of newly synthesized proteins with membranes to regulate essential functions such as cellular signaling and stress responses. This process can be catalyzed during protein synthesis by N-myristoyltransferases (NMTs), and its dysregulation has been implicated both in cancer and heart disease. Although the nascent polypeptide-associated complex (NAC) orchestrates the binding of several co-translational processing factors on ribosomes, its role in facilitating nascent protein myristoylation by NMT2 remains unclear. Here, we show that NAC mediates binding of NMT2 to translating ribosomes, which together form an extended channel that guides the nascent chain as it emerges from the polypeptide exit tunnel to the catalytic site of NMT2. Furthermore, the ternary ribosome:NMT2:NAC complex is stabilized by a ribosomal RNA clamp that, together with NAC, orients NMT2 on the ribosomal surface for co-translational myristoylation of nascent chains. Our work uncovers the molecular mechanism coupling protein synthesis to nascent protein myristoylation and underscores the role of NAC as a master regulator of protein biogenesis on the ribosome.
In the structure databanks used in Yorodumi, some data are registered as the other names, "COVID-19 virus" and "2019-nCoV". Here are the details of the virus and the list of structure data.
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