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- EMDB-49252: In-situ structure of the flagellar motor of Campylobacter jejuni ... -

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Basic information

Entry
Database: EMDB / ID: EMD-49252
TitleIn-situ structure of the flagellar motor of Campylobacter jejuni fcpMNO deletion mutant
Map dataIn-situ structure of the flagellar motor of Campylobacter jejuni fcpMNO deletion mutant
Sample
  • Cell: Campylobacter jejuni
KeywordsC. jejuni / Flagella / Complex / Flagellar motor / MOTOR PROTEIN
Biological speciesCampylobacter jejuni (Campylobacter)
Methodsubtomogram averaging / cryo EM / Resolution: 32.6 Å
AuthorsTachiyama S / Liu J
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI087946 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI132818 United States
CitationJournal: Nat Microbiol / Year: 2026
Title: Structural insights into the assembly and evolution of a complex bacterial flagellar motor.
Authors: Xueyin Feng / Shoichi Tachiyama / Jing He / Siqi Zhu / Hang Zhao / Jack M Botting / Yanran Liu / Yuanyuan Chen / Canfeng Hua / María Lara-Tejero / Matthew A B Baker / Xiang Gao / Jun Liu / Beile Gao /
Abstract: Knowledge of bacterial flagella has largely come from studies of the simpler motors of Escherichia coli and Salmonella enterica. However, many bacteria harbour more complex motors. The function, ...Knowledge of bacterial flagella has largely come from studies of the simpler motors of Escherichia coli and Salmonella enterica. However, many bacteria harbour more complex motors. The function, mechanisms and evolution associated with such auxiliary motor structures are unclear. Here we deploy structural, genetic, biochemical and functional approaches to characterize complex adaptations of the flagellar motor in Campylobacter jejuni. We observed an E ring formed by 17 FlgY homodimers around the MS ring, a cage-like structure made of FcpMNO and PflD, and PflA-PflB interactions in a spoke-rim formation between the E ring and cage. These scaffolds stabilized the 17 torque-generating stator complexes. Phylogenetic analyses suggest an ancient origin and widespread prevalence of the E ring and spokes across diverse flagellated bacteria, and co-option of type IV pilus components in the ancestral motor of phylum Campylobacterota. Collectively, these data provide insight into the assembly, function and evolution of complex flagellar motors.
History
DepositionFeb 16, 2025-
Header (metadata) releaseDec 17, 2025-
Map releaseDec 17, 2025-
UpdateJan 21, 2026-
Current statusJan 21, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_49252.png

Downloads & links

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Map

FileDownload / File: emd_49252.map.gz / Format: CCP4 / Size: 59.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationIn-situ structure of the flagellar motor of Campylobacter jejuni fcpMNO deletion mutant
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
4.3 Å/pix.
x 250 pix.
= 1074. Å		4.3 Å/pix.
x 250 pix.
= 1074. Å		4.3 Å/pix.
x 250 pix.
= 1074. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 4.296 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0687
Minimum - Maximum-0.46268803 - 0.6509079
Average (Standard dev.)0.000000000014852 (±0.06557787)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions250250250
Spacing250250250
CellA=B=C: 1074.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Half map of the flagellar motor structure of...

Fileemd_49252_half_map_1.map
AnnotationHalf map of the flagellar motor structure of Campylobacter jejuni fcpMNO deletion mutant
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map of the flagellar motor structure of...

Fileemd_49252_half_map_2.map
AnnotationHalf map of the flagellar motor structure of Campylobacter jejuni fcpMNO deletion mutant
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Campylobacter jejuni

EntireName: Campylobacter jejuni (Campylobacter)
Components
  • Cell: Campylobacter jejuni

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Supramolecule #1: Campylobacter jejuni

SupramoleculeName: Campylobacter jejuni / type: cell / ID: 1 / Parent: 0
Source (natural)Organism: Campylobacter jejuni (Campylobacter)

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE-PROPANE

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Electron microscopy

MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 1.96 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 5.0 µm / Nominal defocus min: 3.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Point group: C17 (17 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 32.6 Å / Resolution method: FSC 0.5 CUT-OFF / Number subtomograms used: 196
ExtractionNumber tomograms: 129 / Number images used: 199
CTF correctionType: PHASE FLIPPING ONLY
Final angle assignmentType: OTHER

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