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- EMDB-4917: Structure of the dynein-2 complex; motor domains -

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Basic information

Entry
Database: EMDB / ID: EMD-4917
TitleStructure of the dynein-2 complex; motor domains
Map dataMotor domain map, sharpened and masked
Sample
  • Complex: Dynein-2 complex; motor domains
    • Protein or peptide: O6-alkylguanine-DNA alkyltransferase mutant,DYNC2H1 variant protein
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION
Function / homology
Function and homology information


organelle organization / methylated-DNA-[protein]-cysteine S-methyltransferase / methylated-DNA-[protein]-cysteine S-methyltransferase activity / DNA modification / cell projection organization / dynein complex / minus-end-directed microtubule motor activity / dynein light intermediate chain binding / dynein intermediate chain binding / microtubule-based movement ...organelle organization / methylated-DNA-[protein]-cysteine S-methyltransferase / methylated-DNA-[protein]-cysteine S-methyltransferase activity / DNA modification / cell projection organization / dynein complex / minus-end-directed microtubule motor activity / dynein light intermediate chain binding / dynein intermediate chain binding / microtubule-based movement / cilium / methylation / microtubule / DNA repair / ATP hydrolysis activity / DNA binding / ATP binding / metal ion binding / nucleus / plasma membrane / cytoplasm
Similarity search - Function
: / Cytoplasmic dynein 2 heavy chain 1, AAA+ ATPase domain / Methylguanine DNA methyltransferase, ribonuclease-like domain / 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain / Methylated DNA-protein cysteine methyltransferase domain superfamily / Methylated-DNA-[protein]-cysteine S-methyltransferase, active site / Methylated-DNA--protein-cysteine methyltransferase active site. / Methylated-DNA-[protein]-cysteine S-methyltransferase, DNA binding / Methylated DNA-protein cysteine methyltransferase, DNA binding domain / 6-O-methylguanine DNA methyltransferase, DNA binding domain ...: / Cytoplasmic dynein 2 heavy chain 1, AAA+ ATPase domain / Methylguanine DNA methyltransferase, ribonuclease-like domain / 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain / Methylated DNA-protein cysteine methyltransferase domain superfamily / Methylated-DNA-[protein]-cysteine S-methyltransferase, active site / Methylated-DNA--protein-cysteine methyltransferase active site. / Methylated-DNA-[protein]-cysteine S-methyltransferase, DNA binding / Methylated DNA-protein cysteine methyltransferase, DNA binding domain / 6-O-methylguanine DNA methyltransferase, DNA binding domain / Dynein heavy chain, C-terminal domain / Dynein heavy chain, C-terminal domain, barrel region / Dynein heavy chain C-terminal domain / P-loop containing dynein motor region / Dynein heavy chain, tail / Dynein heavy chain, N-terminal region 1 / Dynein heavy chain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Winged helix-like DNA-binding domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Cytoplasmic dynein 2 heavy chain 1 / Methylated-DNA--protein-cysteine methyltransferase
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsToropova K / Zalyte R / Mukhopadhyay AG / Mladenov M / Carter AP / Roberts AJ
Funding support United Kingdom, 8 items
OrganizationGrant numberCountry
Wellcome Trust104196/Z/14/Z United Kingdom
Wellcome TrustWT100387 United Kingdom
Royal Society104196/Z/14/Z United Kingdom
Royal SocietyRG170260 United Kingdom
Biotechnology and Biological Sciences Research CouncilBB/P008348/1 United Kingdom
Wellcome Trust079605/Z/06/Z United Kingdom
Medical Research Council (United Kingdom)MC_UP_A025_1011 United Kingdom
Biotechnology and Biological Sciences Research CouncilBB/L014211/1 United Kingdom
CitationJournal: Nat Struct Mol Biol / Year: 2019
Title: Structure of the dynein-2 complex and its assembly with intraflagellar transport trains.
Authors: Katerina Toropova / Ruta Zalyte / Aakash G Mukhopadhyay / Miroslav Mladenov / Andrew P Carter / Anthony J Roberts /
Abstract: Dynein-2 assembles with polymeric intraflagellar transport (IFT) trains to form a transport machinery that is crucial for cilia biogenesis and signaling. Here we recombinantly expressed the ~1.4-MDa ...Dynein-2 assembles with polymeric intraflagellar transport (IFT) trains to form a transport machinery that is crucial for cilia biogenesis and signaling. Here we recombinantly expressed the ~1.4-MDa human dynein-2 complex and solved its cryo-EM structure to near-atomic resolution. The two identical copies of the dynein-2 heavy chain are contorted into different conformations by a WDR60-WDR34 heterodimer and a block of two RB and six LC8 light chains. One heavy chain is steered into a zig-zag conformation, which matches the periodicity of the anterograde IFT-B train. Contacts between adjacent dyneins along the train indicate a cooperative mode of assembly. Removal of the WDR60-WDR34-light chain subcomplex renders dynein-2 monomeric and relieves autoinhibition of its motility. Our results converge on a model in which an unusual stoichiometry of non-motor subunits controls dynein-2 assembly, asymmetry, and activity, giving mechanistic insight into the interaction of dynein-2 with IFT trains and the origin of diverse functions in the dynein family.
History
DepositionMay 1, 2019-
Header (metadata) releaseJul 24, 2019-
Map releaseAug 28, 2019-
UpdateDec 2, 2020-
Current statusDec 2, 2020Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.042
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 0.042
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6rla
  • Surface level: 0.042
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4917.png

Downloads & links

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Map

FileDownload / File: emd_4917.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMotor domain map, sharpened and masked
Voxel sizeX=Y=Z: 1.39 Å
Density
Contour LevelBy AUTHOR: 0.042 / Movie #1: 0.042
Minimum - Maximum-0.10413136 - 0.20333588
Average (Standard dev.)0.0006723683 (±0.006549925)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions280280280
Spacing280280280
CellA=B=C: 389.19998 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.391.391.39
M x/y/z280280280
origin x/y/z0.0000.0000.000
length x/y/z389.200389.200389.200
α/β/γ90.00090.00090.000
start NX/NY/NZ-39-149-104
NX/NY/NZ201201211
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS280280280
D min/max/mean-0.1040.2030.001

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Supplemental data

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Mask #1

Fileemd_4917_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Motor domain map, unsharpened and unmasked

Fileemd_4917_additional.map
AnnotationMotor domain map, unsharpened and unmasked
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Motor domain half map 2, unmasked

Fileemd_4917_half_map_1.map
AnnotationMotor domain half map 2, unmasked
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Motor domain half map 1, unmasked

Fileemd_4917_half_map_2.map
AnnotationMotor domain half map 1, unmasked
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Dynein-2 complex; motor domains

EntireName: Dynein-2 complex; motor domains
Components
  • Complex: Dynein-2 complex; motor domains
    • Protein or peptide: O6-alkylguanine-DNA alkyltransferase mutant,DYNC2H1 variant protein
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION

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Supramolecule #1: Dynein-2 complex; motor domains

SupramoleculeName: Dynein-2 complex; motor domains / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)

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Macromolecule #1: O6-alkylguanine-DNA alkyltransferase mutant,DYNC2H1 variant protein

MacromoleculeName: O6-alkylguanine-DNA alkyltransferase mutant,DYNC2H1 variant protein
type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 515.223031 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: GDKDCEMKRT TLDSPLGKLE LSGCEQGLHR IIFLGKGTSA ADAVEVPAPA AVLGGPEPLM QATAWLNAYF HQPEAIEEFP VPALHHPVF QQESFTRQVL WKLLKVVKFG EVISYSHLAA LAGNPAATAA VKTALSGNPV PILIPCHRVV QGDLDVGGYE G GLAVKEWL ...String:
GDKDCEMKRT TLDSPLGKLE LSGCEQGLHR IIFLGKGTSA ADAVEVPAPA AVLGGPEPLM QATAWLNAYF HQPEAIEEFP VPALHHPVF QQESFTRQVL WKLLKVVKFG EVISYSHLAA LAGNPAATAA VKTALSGNPV PILIPCHRVV QGDLDVGGYE G GLAVKEWL LAHEGHRLGK PGLGGSLEVL FQGPDYDIPT TLEVLFQGPA NGTADVRKLF IFTTTQNYFG LMSELWDQPL LC NCLEINN FLDDGNQMLL RVQRSDAGIS FSNTIEFGDT KDKVLVFFKL RPEVITDENL HDNILVSSML ESPISSLYQA VRQ VFAPML LKDQEWSRNF DPKLQNLLSE LEAGLGIVLR RSDTNLTKLK FKEDDTRGIL TPSDEFQFWI EQAHRGNKQI SKER ANYFK ELFETIAREF YNLDSLSLLE VVDLVETTQD VVDDVWRQTE HDHYPESRML HLLDIIGGSF GRFVQKKLGT LNLWE DPYY LVKESLKAGI SICEQWVIVC NHLTGQVWQR YVPHPWKNEK YFPETLDKLG KRLEEVLAIR TIHEKFLYFL PASEEK IIC LTRVFEPFTG LNPVQYNPYT EPLWKAAVSQ YEKIIAPAEQ KIAGKLKNYI SEIQDSPQQL LQAFLKYKEL VKRPTIS KE LMLERETLLA RLVDSIKDFR LDFENRCRGI PGDASGPLSG KNLSEVVNSI VWVRQLELKV DDTIKIAEAL LSDLPGFR C FHQSAKDLLD QLKLYEQEQF DDWSRDIQSG LSDSRSGLCI EASSRIMELD SNDGLLKVHY SDRLVILLRE VRQLSALGF VIPAKIQQVA NIAQKFCKQA IILKQVAHFY NSIDQQMIQS QRPMMLQSAL AFEQIIKNSK AGSGGKSQIT WDNPKELEGY IQKLQNAAE RLATENRKLR KWHTTFCEKV VVLMNIDLLR QQQRWKDGLQ ELRTGLATVE AQGFQASDMH AWKQHWNHQL Y KALEHQYQ MGLEALNENL PEINIDLTYK QGRLQFRPPF EEIRAKYYRE MKRFIGIPNQ FKGVGEAGDE SIFSIMIDRN AS GFLTIFS KAEDLFRRLS AVLHQHKEWI VIGQVDMEAL VEKHLFTVHD WEKNFKALKI KGKEVERLPS AVKVDCLNIN CNP VKTVID DLIQKLFDLL VLSLKKSIQA HLHEIDTFVT EAMEVLTIMP QSVEEIGDAN LQYSKLQERK PEILPLFQEA EDKN RLLRT VAGGGLETIS NLKAKWDKFE LMMESHQLMI KDQIEVMKGN VKSRLQIYYQ ELEKFKARWD QLKPGDDVIE TGQHN TLDK SAKLIKEKKI EFDDLEVTRK KLVDDCHHFR LEEPNFSLAS SISKDIESCA QIWAFYEEFQ QGFQEMANED WITFRT KTY LFEEFLMNWH DRLRKVEEHS VMTVKLQSEV DKYKIVIPIL KYVRGEHLSP DHWLDLFRLL GLPRGTSLEK LLFGDLL RV ADTIVAKAAD LKDLNSRAQG EVTIREALRE LDLWGVGAVF TLIDYEDSQS RTMKLIKDWK DIVNQVGDNR CLLQSLKD S PYYKGFEDKV SIWERKLAEL DEYLQNLNHI QRKWVYLEPI FGRGALPKEQ TRFNRVDEDF RSIMTDIKKD NRVTTLTTH AGIRNSLLTI LDQLQRCQRS LNEFLEEKRS AFPRFYFIGD DDLLEILGQS TNPSVIQSHL KKLFAGINSV CFDEKSKHIT AMKSLEGEV VPFKNKVPLS NNVETWLNDL ALEMKKTLEQ LLKECVTTGR SSQGAVDPSL FPSQILCLAE QIKFTEDVEN A IKDHSLHQ IETQLVNKLE QYTNIDTSSE DPGNTESGIL ELKLKALILD IIHNIDVVKQ LNQIQVHTTE DWAWKKQLRF YM KSDHTCC VQMVDSEFQY TYEYQGNASK LVYTPLTDKC YLTLTQAMKM GLGGNPYGPA GTGKTESVKA LGGLLGRQVL VFN CDEGID VKSMGRIFVG LVKCGAWGCF DEFNRLEESV LSAVSMQIQT IQDALKNHRT VCELLGKEVE VNSNSGIFIT MNPA GKGYG GRQKLPDNLK QLFRPVAMSH PDNELIAEVI LYSEGFKDAK VLSRKLVAIF NLSRELLTPQ QHYDWGLRAL KTVLR GSGN LLRQLNKSGT TQNANESHIV VQALRLNTMS KFTFTDCTRF DALIKDVFPG IELKEVEYDE LSAALKQVFE EANYEI IPN QIKKALELYE QLCQRMGVVI VGPSGAGKST LWRMLRAALC KTGKVVKQYT MNPKAMPRYQ LLGHIDMDTR EWSDGVL TN SARQVVREPQ DVSSWIICDG DIDPEWIESL NSVLDDNRLL TMPSGERIQF GPNVNFVFET HDLSCASPAT ISRMGMIF L SDEETDLNSL IKSWLRNQPA EYRNNLENWI GDYFEKALQW VLKQNDYVVE TSLVGTVMNG LSHLHGCRDH DEFIINLIR GLGGNLNMKS RLEFTKEVFH WARESPPDFH KPMDTYYDST RGRLATYVLK KPEDLTADDF SNGLTLPVIQ TPDMQRGLDY FKPWLSSDT KQPFILVGPE GCGKGMLLRY AFSQLRSTQI ATVHCSAQTT SRHLLQKLSQ TCMVISTNTG RVYRPKDCER L VLYLKDIN LPKLDKWGTS TLVAFLQQVL TYQGFYDENL EWVGLENIQI VASMSAGGRL GRHKLTTRFT SIVRLCSIDY PE REQLQTI YGAYLEPVLH KNLKNHSIWG SSSKIYLLAG SMVQVYEQVR AKFTVDDYSH YFFTPCILTQ WVLGLFRYDL EGG SSNHPL DYVLEIVAYE ARRLFRDKIV GAKELHLFDI ILTSVFQGDW GSDILDNMSD SFYVTWGARH NSGARAAPGQ PLPP HGKPL GKLNSTDLKD VIKKGLIHYG RDNQNLDILL FHEVLEYMSR IDRVLSFPGG SLLLAGRSGV GRRTITSLVS HMHGA VLFS PKISRGYELK QFKNDLKHVL QLAGIEAQQV VLLLEDYQFV HPTFLEMINS LLSSGEVPGL YTLEELEPLL LPLKDQ ASQ DGFFGPVFNY FTYRIQQNLH IVLIMDSANS NFMINCESNP ALHKKCQVLW MEGWSNSSMK KIPEMLFSET GGGEKYN DK KRKEEKKKNS VDPDFLKSFL LIHESCKAYG ATPSQYMTFL HVYSAISSSK KKELLKRQSH LQAGVSKLNE AKALVDEL N RKAGEQSVLL KTKQDEADAA LQMITVSMQD ASEQKTELER LKHRIAEEVV KIEERKNKID DELKEVQPLV NEAKLAVGN IKPESLSEIR SLRMPPDVIR DILEGVLRLM GIFDTSWVSM KSFLAKRGVR EDIATFDARN ISKEIRESVE ELLFKNKGSF DPKNAKRAS TAAAPLAAWV KANIQYSHVL ERIHPLETEQ AGLESNLKKT EDRKRKLEEL LNSVGQKVSE LKEKFQSRTS E AAKLEAEV SKAQETIKAA EVLINQLDRE HKRWNAQVVE ITEELATLPK RAQLAAAFIT YLSAAPESLR KTCLEEWTKS AG LEKFDLR RFLCTESEQL IWKSEGLPSD DLSIENALVI LQSRVCPFLI DPSSQATEWL KTHLKDSRLE VINQQDSNFI TAL ELAVRF GKTLIIQEMD GVEPVLYPLL RRDLVAQGPR YVVQIGDKII DYNEEFRLFL STRNPNPFIP PDAASIVTEV NFTT TRSGL RGQLLALTIQ HEKPDLEEQK TKLLQQEEDK KIQLAKLEES LLETLATSQG NILENKDLIE SLNQTKASSA LIQES LKES YKLQISLDQE RDAYLPLAES ASKMYFIISD LSKINNMYRF SLAAFLRLFQ RALQNKQDSE NTEQRIQSLI SSLQHM VYE YICRCLFKAD QLMFALHFVR GMHPELFQEN EWDTFTGVVV GDMLRKADSQ QKIRDQLPSW IDQERSWAVA TLKIALP SL YQTLCFEDAA LWRTYYNNSM CEQEFPSILA KKVSLFQQIL VVQVLRPDRL QSAMALFACK TLGLKEVSPL PLNLKRLY K ETLEIEPILI IISPGADPSQ ELQELANAER SGECYHQVAM GQGQADLAIQ MLKECARNGD WLCLKNLHLV VSWLPVLEK ELNTLQPKDT FRLWLTAEVH PNFTPILLQS SLKITYESPP GLKKNLMRTY ESWTPEQISK KDNTHRAHAL FSLAWFHAAC QERRNYIPQ GWTKFYEFSL SDLRAGYNII DRLFDGAKDV QWEFVHGLLE NAIYGGRIDN YFDLRVLQSY LKQFFNSSVI D VFNQRNKK SIFPYSVSLP QSCSILDYRA VIEKIPEDDK PSFFGLPANI ARSSQRMISS QVISQLRILG RSITAGSKFD RE IWSNELS PVLNLWKKLN QNSNLIHQKV PPPNDRQGSP ILSFIILEQF NAIRLVQSVH QSLAALSKVI RGTTLLSSEV QKL ASALLN QKCPLAWQSK WEGPEDPLQY LRGLVARALA IQNWVDKAEK QALLSETLDL SELFHPDTFL NALRQETARA VGRS VDSLK FVASWKGRLQ EAKLQIKISG LLLEGCSFDG NQLSENQLDS PSVSSVLPCF MGWIPQDACG PYSPDECISL PVYTS AERD RVVTNIDVPC GGNQDQWIQC GAALFLKNQ

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Macromolecule #2: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 2 / Number of copies: 6 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

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Macromolecule #3: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 3 / Number of copies: 2 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

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Macromolecule #4: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 4 / Number of copies: 2 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
GridMaterial: COPPER / Support film - Material: CARBON / Support film - topology: LACEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 105000
Specialist opticsEnergy filter - Slit width: 20 eV
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 49.6 e/Å2
Details: Average electron dose per image (e-/A2) for additional datasets was 46.8 and 45.4
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware: (Name: Gctf, RELION (ver. 2.1))
Startup modelType of model: INSILICO MODEL
In silico model: Model of auto-inhibited state of dynein-2 motor domains from Toropova et al., 2017 Nat. Struct. Mol. Biol., derived from PDB entry 4RH7.
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
Final reconstructionApplied symmetry - Point group: C2 (2 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.1) / Number images used: 57265
FSC plot (resolution estimation)

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