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データを開く
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基本情報
登録情報 | ![]() | |||||||||||||||
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タイトル | Damaged 70S ribosome with PrfH bound | |||||||||||||||
![]() | cryo-EM map of damage 70S E. coli Ribosome rescured by C. gingivalis PrfH | |||||||||||||||
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![]() | CgPrfH / damaged ribosome / ribotoxin / RIBOSOME | |||||||||||||||
機能・相同性 | ![]() negative regulation of cytoplasmic translational initiation / stringent response / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / translational termination / negative regulation of cytoplasmic translation / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation ...negative regulation of cytoplasmic translational initiation / stringent response / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / translational termination / negative regulation of cytoplasmic translation / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / ribosome assembly / transcription antitermination / translational initiation / DNA-templated transcription termination / response to radiation / maintenance of translational fidelity / mRNA 5'-UTR binding / large ribosomal subunit / ribosome binding / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / small ribosomal subunit rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / response to antibiotic / negative regulation of DNA-templated transcription / mRNA binding / DNA binding / RNA binding / zinc ion binding / membrane / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||||||||
生物種 | ![]() ![]() ![]() | |||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.65 Å | |||||||||||||||
![]() | Tian Y / Li Q / Jin H / Fatma S / Zeng F / Huang RH | |||||||||||||||
資金援助 | ![]() ![]()
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![]() | ![]() タイトル: Molecular and structural basis of a subfamily of PrfH rescuing both the damaged and intact ribosomes stalled in translation. 著者: Yannan Tian / Qingrong Li / Shirin Fatma / Junyi Jiang / Hong Jin / Fuxing Zeng / Raven H Huang / ![]() ![]() 要旨: In bacteria, spontaneous mRNAs degradation and ribotoxin-induced RNA damage are two main biological events that lead to the stall of protein translation. The ubiquitous trans-translation system as ...In bacteria, spontaneous mRNAs degradation and ribotoxin-induced RNA damage are two main biological events that lead to the stall of protein translation. The ubiquitous trans-translation system as well as several alternative rescue factors (Arfs) are responsible for rescuing the stalled ribosomes caused by truncated mRNAs that lack the stop codons. To date, protein release factor homolog (PrfH) is the only factor known to rescue the stalled ribosome damaged by ribotoxins. Here we show that a subfamily of PrfH, exemplified by PrfH from (PrfH), rescues both types of stalled ribosomes described above. Our biochemical assays demonstrate that PrfH hydrolyzes the peptides attached to P-site tRNAs when in complex with both the damaged and intact ribosomes. Two cryo-EM structures of PrfH in complex with the damaged and intact 70S ribosomes revealed that PrfH employs two different regions of the protein to recognize two different stalled ribosomes to orient the GGQ motif for peptide hydrolysis. Thus, using a combination of bioinformatic, biochemical, and structural characterization described here, we have uncovered a family of ribosome rescue factors that possesses dual activities to resolve two distinct stalled protein translation in bacteria. | |||||||||||||||
履歴 |
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 204 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 95 KB 95 KB | 表示 表示 | ![]() |
画像 | ![]() | 88.8 KB | ||
Filedesc metadata | ![]() | 23.9 KB | ||
その他 | ![]() ![]() | 200.8 MB 200.8 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 1 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1 MB | 表示 | |
XML形式データ | ![]() | 15.8 KB | 表示 | |
CIF形式データ | ![]() | 18.8 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 9morMC ![]() 9mq4C M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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注釈 | cryo-EM map of damage 70S E. coli Ribosome rescured by C. gingivalis PrfH | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.095 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : Damaged 70S with PrfH
+超分子 #1: Damaged 70S with PrfH
+超分子 #2: 50S
+超分子 #3: 30S
+超分子 #4: tRNA
+超分子 #5: Putative peptide chain release factor homolog
+分子 #1: 23S ribosomal RNA
+分子 #2: 16S ribosomal RNA
+分子 #3: 5S ribosomal RNA
+分子 #4: P-tRNA, E-tRNA
+分子 #5: mRNA
+分子 #6: Peptide chain release factor H
+分子 #7: 50S ribosomal protein L2
+分子 #8: 50S ribosomal protein L3
+分子 #9: Large ribosomal subunit protein uL4
+分子 #10: Large ribosomal subunit protein uL5
+分子 #11: Large ribosomal subunit protein uL6
+分子 #12: Large ribosomal subunit protein bL9
+分子 #13: 50S ribosomal protein L10
+分子 #14: 50S ribosomal protein L11
+分子 #15: Large ribosomal subunit protein uL13
+分子 #16: Large ribosomal subunit protein uL14
+分子 #17: Large ribosomal subunit protein uL15
+分子 #18: 50S ribosomal protein L16
+分子 #19: Large ribosomal subunit protein bL17
+分子 #20: Large ribosomal subunit protein uL18
+分子 #21: Large ribosomal subunit protein bL19
+分子 #22: 50S ribosomal protein L20
+分子 #23: Large ribosomal subunit protein bL21
+分子 #24: Large ribosomal subunit protein uL22
+分子 #25: 50S ribosomal protein L23
+分子 #26: 50S ribosomal protein L24
+分子 #27: Large ribosomal subunit protein bL25
+分子 #28: Large ribosomal subunit protein bL27
+分子 #29: 50S ribosomal protein L28
+分子 #30: Large ribosomal subunit protein uL29
+分子 #31: 50S ribosomal protein L30
+分子 #32: 50S ribosomal protein L31
+分子 #33: 50S ribosomal protein L32
+分子 #34: 50S ribosomal protein L33
+分子 #35: 50S ribosomal protein L34
+分子 #36: 50S ribosomal protein L35
+分子 #37: 50S ribosomal protein L36
+分子 #38: 30S ribosomal protein S2
+分子 #39: 30S ribosomal protein S3
+分子 #40: 30S ribosomal protein S4
+分子 #41: 30S ribosomal protein S5
+分子 #42: 30S ribosomal protein S6
+分子 #43: 30S ribosomal protein S7
+分子 #44: Small ribosomal subunit protein uS8
+分子 #45: Small ribosomal subunit protein uS9
+分子 #46: Small ribosomal subunit protein uS10
+分子 #47: 30S ribosomal protein S11
+分子 #48: Small ribosomal subunit protein uS12
+分子 #49: 30S ribosomal protein S13
+分子 #50: Small ribosomal subunit protein uS14
+分子 #51: Small ribosomal subunit protein uS15
+分子 #52: 30S ribosomal protein S16
+分子 #53: Small ribosomal subunit protein uS17
+分子 #54: 30S ribosomal protein S18
+分子 #55: Small ribosomal subunit protein uS19
+分子 #56: 30S ribosomal protein S20
+分子 #57: 30S ribosomal protein S21
+分子 #58: MAGNESIUM ION
+分子 #59: ZINC ION
+分子 #60: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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グリッド | モデル: Quantifoil R1.2/1.3 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 0.4 / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 30 sec. |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | TFS KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 検出モード: SUPER-RESOLUTION / 平均電子線量: 30.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 1.0 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |