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- PDB-9mq4: Damaged 70S ribosome with PrfH bound -

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Basic information

Entry
Database: PDB / ID: 9mq4
TitleDamaged 70S ribosome with PrfH bound
Components
  • (30S ribosomal protein ...) x 11
  • (50S ribosomal protein ...) x 16
  • (Large ribosomal subunit protein ...) x 15
  • (Small ribosomal subunit protein ...) x 9
  • 16S ribosomal RNA
  • 23S ribosomal RNA
  • 5S ribosomal RNA
  • P-tRNA, E-tRNA
  • Peptide chain release factor H
  • mRNA
KeywordsRIBOSOME / CgPrfH / damaged ribosome / ribotoxin
Function / homology
Function and homology information


negative regulation of cytoplasmic translational initiation / stringent response / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / translational termination / negative regulation of cytoplasmic translation / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation ...negative regulation of cytoplasmic translational initiation / stringent response / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / translational termination / negative regulation of cytoplasmic translation / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / ribosome assembly / transcription antitermination / translational initiation / DNA-templated transcription termination / response to radiation / maintenance of translational fidelity / mRNA 5'-UTR binding / large ribosomal subunit / transferase activity / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / response to antibiotic / negative regulation of DNA-templated transcription / mRNA binding / DNA binding / RNA binding / zinc ion binding / membrane / cytosol / cytoplasm
Similarity search - Function
Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L10 / : / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L11, bacterial-type / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 type A ...Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L10 / : / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L11, bacterial-type / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein S21 / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / : / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L9 signature. / : / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L17 signature. / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / : / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / : / Ribosomal protein S19, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily
Similarity search - Domain/homology
: / : / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein bS21 ...: / : / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein bL20 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL33 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein bL21
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
Escherichia coli 0.1197 (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.78 Å
AuthorsTian, Y. / Li, Q. / Jin, H. / Fatma, S. / Zeng, F. / Huang, R.H.
Funding support United States, China, 4items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM145060 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM120764 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM120552 United States
National Natural Science Foundation of China (NSFC)32171200 China
CitationJournal: bioRxiv / Year: 2025
Title: Molecular and structural basis of a subfamily of PrfH rescuing both the damaged and intact ribosomes stalled in translation.
Authors: Yannan Tian / Qingrong Li / Shirin Fatma / Junyi Jiang / Hong Jin / Fuxing Zeng / Raven H Huang /
Abstract: In bacteria, spontaneous mRNAs degradation and ribotoxin-induced RNA damage are two main biological events that lead to the stall of protein translation. The ubiquitous trans-translation system as ...In bacteria, spontaneous mRNAs degradation and ribotoxin-induced RNA damage are two main biological events that lead to the stall of protein translation. The ubiquitous trans-translation system as well as several alternative rescue factors (Arfs) are responsible for rescuing the stalled ribosomes caused by truncated mRNAs that lack the stop codons. To date, protein release factor homolog (PrfH) is the only factor known to rescue the stalled ribosome damaged by ribotoxins. Here we show that a subfamily of PrfH, exemplified by PrfH from (PrfH), rescues both types of stalled ribosomes described above. Our biochemical assays demonstrate that PrfH hydrolyzes the peptides attached to P-site tRNAs when in complex with both the damaged and intact ribosomes. Two cryo-EM structures of PrfH in complex with the damaged and intact 70S ribosomes revealed that PrfH employs two different regions of the protein to recognize two different stalled ribosomes to orient the GGQ motif for peptide hydrolysis. Thus, using a combination of bioinformatic, biochemical, and structural characterization described here, we have uncovered a family of ribosome rescue factors that possesses dual activities to resolve two distinct stalled protein translation in bacteria.
History
DepositionJan 1, 2025Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 5, 2025Provider: repository / Type: Initial release
Revision 1.0Feb 5, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
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Item: _chem_comp_atom.atom_id / _chem_comp_atom.pdbx_aromatic_flag ..._chem_comp_atom.atom_id / _chem_comp_atom.pdbx_aromatic_flag / _chem_comp_bond.atom_id_1 / _chem_comp_bond.atom_id_2 / _chem_comp_bond.pdbx_aromatic_flag / _chem_comp_bond.value_order / _em_admin.last_update
Revision 1.1Mar 19, 2025Data content type: EM metadata / Data content type: EM metadata / Group: Experimental summary / Data content type: EM metadata / Category: em_admin / Data content type: EM metadata / Item: _em_admin.last_update

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
1: 23S ribosomal RNA
2: 16S ribosomal RNA
4: 5S ribosomal RNA
5: P-tRNA, E-tRNA
6: P-tRNA, E-tRNA
7: mRNA
A: Peptide chain release factor H
B: 50S ribosomal protein L2
C: 50S ribosomal protein L3
D: Large ribosomal subunit protein uL4
E: Large ribosomal subunit protein uL5
F: Large ribosomal subunit protein uL6
G: Large ribosomal subunit protein bL9
H: 50S ribosomal protein L10
I: 50S ribosomal protein L11
J: Large ribosomal subunit protein uL13
K: Large ribosomal subunit protein uL14
L: Large ribosomal subunit protein uL15
M: 50S ribosomal protein L16
N: Large ribosomal subunit protein bL17
O: Large ribosomal subunit protein uL18
P: Large ribosomal subunit protein bL19
Q: 50S ribosomal protein L20
R: Large ribosomal subunit protein bL21
S: Large ribosomal subunit protein uL22
T: 50S ribosomal protein L23
U: 50S ribosomal protein L24
V: Large ribosomal subunit protein bL25
W: Large ribosomal subunit protein bL27
X: 50S ribosomal protein L28
Y: Large ribosomal subunit protein uL29
Z: 50S ribosomal protein L30
a: 50S ribosomal protein L31
b: 50S ribosomal protein L32
c: 50S ribosomal protein L33
d: 50S ribosomal protein L34
e: 50S ribosomal protein L35
f: 50S ribosomal protein L36
g: 30S ribosomal protein S2
h: 30S ribosomal protein S3
i: 30S ribosomal protein S4
j: Small ribosomal subunit protein uS5
k: 30S ribosomal protein S6
l: 30S ribosomal protein S7
m: Small ribosomal subunit protein uS8
n: Small ribosomal subunit protein uS9
o: Small ribosomal subunit protein uS10
p: 30S ribosomal protein S11
q: Small ribosomal subunit protein uS12
r: 30S ribosomal protein S13
s: Small ribosomal subunit protein uS14
t: Small ribosomal subunit protein uS15
u: 30S ribosomal protein S16
v: Small ribosomal subunit protein uS17
w: 30S ribosomal protein S18
x: Small ribosomal subunit protein uS19
y: 30S ribosomal protein S20
z: 30S ribosomal protein S21
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,265,297499
Polymers2,254,49658
Non-polymers10,801441
Water362
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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RNA chain , 5 types, 6 molecules 124567

#1: RNA chain 23S ribosomal RNA


Mass: 941526.438 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli)
#2: RNA chain 16S ribosomal RNA


Mass: 497404.969 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: GenBank: 1162858853
#3: RNA chain 5S ribosomal RNA


Mass: 38790.090 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: GenBank: 1273279017
#4: RNA chain P-tRNA, E-tRNA


Mass: 24802.785 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: GenBank: 1848934315
#5: RNA chain mRNA


Mass: 8132.956 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli)

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Protein , 1 types, 1 molecules A

#6: Protein Peptide chain release factor H


Mass: 26394.090 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli)

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50S ribosomal protein ... , 16 types, 16 molecules BCHIMQTUXZabcdef

#7: Protein 50S ribosomal protein L2 / Large ribosomal subunit protein uL2


Mass: 29923.619 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplB, b3317, JW3279 / Production host: Escherichia coli (E. coli) / References: UniProt: P60422
#8: Protein 50S ribosomal protein L3 / Large ribosomal subunit protein uL3


Mass: 22277.535 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplC, b3320, JW3282 / Production host: Escherichia coli (E. coli) / References: UniProt: P60438
#13: Protein 50S ribosomal protein L10 / 50S ribosomal protein L8 / Large ribosomal subunit protein uL10


Mass: 17736.596 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplJ, b3985, JW3948 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7J3
#14: Protein 50S ribosomal protein L11 / Large ribosomal subunit protein uL11


Mass: 14894.362 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplK, relC, b3983, JW3946 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7J7
#18: Protein 50S ribosomal protein L16 / Large ribosomal subunit protein uL16


Mass: 15312.269 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplP, b3313, JW3275 / Production host: Escherichia coli (E. coli) / References: UniProt: P0ADY7
#22: Protein 50S ribosomal protein L20


Mass: 13528.024 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplT / Production host: Escherichia coli (E. coli) / References: UniProt: B7MAS6
#25: Protein 50S ribosomal protein L23


Mass: 11222.160 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rplW, rplW_1, A5U30_004552, A8502_004023, A9X72_02185, ACN81_25520, ACU57_20960, AM464_09220, AW118_24900, AWP47_12645, B6R15_004069, B6R31_004968, BANRA_04328, BCB93_004293, BER14_25190, BF481_ ...Gene: rplW, rplW_1, A5U30_004552, A8502_004023, A9X72_02185, ACN81_25520, ACU57_20960, AM464_09220, AW118_24900, AWP47_12645, B6R15_004069, B6R31_004968, BANRA_04328, BCB93_004293, BER14_25190, BF481_004930, BG944_001133, BGM66_003593, BJI68_08675, BJJ90_02125, BK292_20505, BK383_24645, BKL28_004543, BTB68_004356, BTQ06_10690, BvCmsKKP061_03030, BvCmsSIP010_02685, BXT93_05475, C0P57_002668, C1Q91_004904, C2121_004162, C2M16_25405, C2R31_004633, C3F40_19720, C9E67_02725, C9Z68_22635, CA593_09650, CF22_004849, CG692_20185, CG704_20870, CG831_003649, CIG67_10675, CQ986_004881, CR538_02420, CR539_21900, CR628_001366, CTR35_003630, CV83915_02780, CY655_21320, D1912_09370, D3C88_31150, D3G36_23050, D4M65_20655, D4N09_20255, D9D43_22550, D9E49_05240, D9J61_16735, DD762_23630, DIV22_15175, DNQ45_03840, DNX30_25395, DQ87_004776, DS732_24345, DTL43_20915, DU321_12085, E4K51_21575, E5H86_24440, E6D34_21675, EAI46_07560, EC95NR1_02709, ECs4183, EEP55_004492, EIA08_24040, EIZ93_12385, EN85_004349, EPS76_07130, EPS97_20010, EWK56_25080, ExPECSC038_03640, F7F11_22640, F7N46_23925, F9407_19210, F9413_21150, F9461_25570, F9B07_24600, FEJ01_22430, FIJ20_21110, FJQ40_18390, FKO60_25720, FOI11_019175, FOI11_04005, FPI65_20390, FPS11_25345, FTV92_20630, FV293_22380, FVB16_04955, FWK02_12650, FZN31_02550, FZU14_21905, G3V95_19930, G3W53_21040, G4A38_21595, G4A47_20865, G5603_24670, G8C01_22470, G9448_16990, GAI89_24745, GAJ12_24635, GGB84_004451, GJ11_21470, GKF66_21630, GNW61_16715, GOP25_22790, GP711_23390, GP954_00860, GP965_04380, GP975_01270, GP979_01920, GQA06_03865, GQM04_10650, GQM13_25285, GQM21_11315, GQN34_23350, GQW07_21465, GRO95_20730, GRW05_09145, GRW24_04670, GRW56_00380, GRW57_03455, GTP92_23110, GUC01_21265, H0O72_19500, HCQ42_003932, HEP30_018720, HEP34_004754, HHH44_004519, HI055_004156, HIE29_005203, HJQ60_004995, HLX92_09990, HLZ50_22095, HMV95_19690, HV109_02105, HV146_21755, HV209_14630, HVW04_17450, HVW43_18585, HVY77_02090, HVZ29_19180, HX086_19490, HX136_02110, I0E88_004501, I6H00_20840, I6H02_12075, IFB95_004822, IUC12_004844, J0541_004413, J5U05_004071, JNP96_25390, KV259_004342, KV317_004314, KV371_004328, KV406_004321, KV449_004356, KV455_004319, KV463_004341, KV469_004334, KV499_004405, KV500_004292, NCTC10082_02823, NCTC10089_00486, NCTC10090_03511, NCTC10418_00674, NCTC10429_01077, NCTC10764_03847, NCTC10767_01500, NCTC10865_00642, NCTC10974_00533, NCTC11112_01052, NCTC11124_04730, NCTC11126_02754, NCTC11181_02722, NCTC11341_01905, NCTC12950_00463, NCTC13148_03456, NCTC4450_02459, NCTC7927_00535, NCTC7928_02533, NCTC8009_01493, NCTC8179_05925, NCTC8333_00473, NCTC8500_00318, NCTC8621_00492, NCTC8622_00522, NCTC8959_03277, NCTC8960_03054, NCTC8985_04965, NCTC9001_04236, NCTC9044_01376, NCTC9045_00549, NCTC9073_00398, NCTC9075_00708, NCTC9081_00901, NCTC9117_00740, NCTC9702_00521, NCTC9706_02721, NCTC9962_06781, NEP60_26375, O5851_12245, RG28_21695, SAMEA3752557_01968, TUM18780_04200
Production host: Escherichia coli (E. coli) / References: UniProt: C3SQV2
#26: Protein 50S ribosomal protein L24


Mass: 11339.250 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rplX, A8502_004032, A9X72_02230, ACN81_25475, ACU57_21005, AM464_09175, AW118_24855, AWP47_12690, B6R15_004078, B6R31_004977, BANRA_04319, BCB93_004284, BER14_25145, BF481_004939, BG944_001124, ...Gene: rplX, A8502_004032, A9X72_02230, ACN81_25475, ACU57_21005, AM464_09175, AW118_24855, AWP47_12690, B6R15_004078, B6R31_004977, BANRA_04319, BCB93_004284, BER14_25145, BF481_004939, BG944_001124, BGM66_003602, BJI68_08720, BJJ90_02170, BK292_20460, BK383_24690, BKL28_004552, BTB68_004365, BTQ06_10645, BvCmsKKP061_03021, BvCmsSIP010_02676, BXT93_05520, C0P57_002659, C1Q91_004913, C2121_004171, C2M16_25450, C2R31_004624, C3F40_19765, C9E67_02770, C9Z68_22590, CA593_09695, CF22_004858, CG704_20915, CG831_003640, CIG67_10720, CQ986_004890, CR538_02465, CR539_21855, CR628_001357, CTR35_003621, CV83915_02789, CY655_21275, D4M65_20610, D9D43_22595, DD762_23585, DIV22_15220, DNQ45_03795, DNX30_25440, DQ87_004785, DS732_24300, DTL43_20960, DU321_12040, E4K51_21530, E5H86_24485, E6D34_21720, EAI46_07515, EC95NR1_02700, ECs4174, EEP55_004501, EIA08_23995, EIZ93_12430, EN85_004358, EPS97_20055, ExPECSC038_03649, F7F11_22685, F7N46_23970, F9407_19255, F9413_21195, F9461_25615, F9B07_24645, FEJ01_22475, FIJ20_21155, FJQ40_18345, FKO60_25675, FOI11_019220, FOI11_03960, FPS11_25390, FTV92_20675, FTV93_20860, FV293_22425, FVB16_04910, FWK02_06310, FZN31_02595, FZU14_21860, G3V95_19885, G4A38_21550, G4A47_20820, G5603_24625, G8C01_22515, G9448_17035, GAI89_24790, GAJ12_24680, GJ11_21425, GKF66_21585, GNW61_16670, GOP25_22745, GP954_00905, GP965_04335, GP975_01225, GP979_01965, GQA06_03910, GQM04_10605, GQM13_25240, GQM21_11270, GQW07_21510, GRO95_20775, GRW05_09100, GRW24_04715, GRW56_00335, GRW57_03500, GTP92_23155, GUC01_21220, H0O72_19455, HCQ42_003941, HEP30_018675, HEP34_004763, HHH44_004528, HI055_004147, HJQ60_005004, HLX92_10035, HLZ50_22140, HMV95_19645, HV109_02150, HV146_21710, HV209_14675, HVW04_17495, HVW43_18630, HVY77_02135, HVZ29_19135, HX086_19445, HX136_02155, I0E88_004510, I6H00_20885, I6H02_12030, IUC12_004853, J0541_004404, J5U05_004080, JNP96_25345, KV259_004351, KV317_004323, KV371_004337, KV406_004330, KV449_004365, KV455_004328, KV463_004350, KV469_004343, KV499_004414, KV500_004301, NCTC10082_02832, NCTC10418_00684, NCTC10764_03856, NCTC10865_00652, NCTC10974_00542, NCTC11124_04721, NCTC11181_02731, NCTC11341_01896, NCTC12950_00472, NCTC7922_04049, NCTC7927_00544, NCTC8009_01504, NCTC8179_05935, NCTC8333_00482, NCTC8500_00327, NCTC8621_00501, NCTC8622_00513, NCTC8960_03063, NCTC8985_04975, NCTC9044_01365, NCTC9045_00558, NCTC9077_00597, NCTC9117_00749, NCTC9702_00530, NCTC9706_02730, NEP60_26330, O5851_12290, RG28_21650, SAMEA3752557_01959, TUM18780_04290, WR15_19645
Production host: Escherichia coli (E. coli) / References: UniProt: C3SQZ7
#29: Protein 50S ribosomal protein L28


Mass: 9027.551 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmB, ECVG_03476 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A1X3JA73
#31: Protein 50S ribosomal protein L30


Mass: 6554.820 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpmD, A5U30_004536, A6592_25715, A8499_003316, A8502_004039, A9X72_02265, AC065_15925, ACN81_25440, ACU57_21040, AM464_09140, APX88_16155, AW118_24820, AWP47_12725, B6R12_002823, B6R15_004085, ...Gene: rpmD, A5U30_004536, A6592_25715, A8499_003316, A8502_004039, A9X72_02265, AC065_15925, ACN81_25440, ACU57_21040, AM464_09140, APX88_16155, AW118_24820, AWP47_12725, B6R12_002823, B6R15_004085, B6R17_004958, B6R31_004984, B6R48_005051, B6R87_004961, BANRA_04312, BB540_01660, BER14_25110, BF481_004946, BG944_001117, BGM66_003609, BHS81_19755, BJI68_08755, BJJ90_02205, BK292_20425, BK383_24725, BKL28_004559, BMT50_12295, BMT91_19990, BR158_003774, BTB68_004372, BTQ06_10610, BUO55_004713, BvCmsKKP061_03014, BvCmsSIP010_02669, BXT93_05555, BZL69_25950, C0P57_002652, C1Q91_004920, C2121_004178, C2M16_25485, C2R31_004617, C3F40_19800, C5N07_23570, C9E67_02805, C9Z68_22555, CA593_09730, CCS08_00135, CCU84_002918, CDL36_25590, CF22_004865, CG692_20265, CG704_20950, CG831_003633, CIG67_10755, CQ986_004897, CQB02_00630, CR538_02500, CR539_21820, CR628_001350, CRM83_22925, CTR35_003614, CV83915_02796, CWS33_24860, CX938_003815, CY655_21240, D1912_11425, D3G36_23130, D4M65_20575, D4N09_20175, D6T60_05695, D9D43_22630, D9E34_23605, D9E49_05160, D9H94_20830, D9J03_20055, D9J61_16655, DD762_23550, DIV22_15255, DNQ45_03760, DNX30_25475, DS732_24265, DTL43_20995, DTL90_24695, DTM45_24550, DU321_12005, E0I52_20145, E4K51_21495, E5H86_24520, E6D34_21755, EA239_22795, EAI46_07480, EB534_RS01705, EC95NR1_02693, ECs4167, EIA08_23960, EIZ93_12465, EN85_004365, EPS76_07210, EPS97_20090, ERS085406_04406, ERS139208_04354, EWK56_25000, ExPECSC038_03656, F3P10_05565, F7F11_22720, F7N46_24005, F9413_21230, F9461_25650, F9B07_24680, FA868_22750, FEJ01_22510, FFF58_25450, FGG80_25130, FHD44_21270, FHQ97_20115, FIJ20_21190, FJQ40_18310, FKO60_25640, FN993_004711, FOI11_019255, FOI11_03925, FPI65_20310, FPS11_25425, FTV92_20710, FTV93_20895, FV293_22460, FVB16_04875, FWK02_06275, FZN31_02630, FZU14_21825, G3V95_19850, G3W53_20960, G4A38_21515, G4A47_20785, G5603_24590, G9448_17070, GAI89_24825, GAJ12_24715, GAJ26_22015, GGB84_004467, GIB53_19395, GJ11_21390, GKF52_21245, GKF66_21550, GKF89_18475, GNW61_16635, GOP14_004273, GOP25_22710, GP711_23310, GP954_00940, GP965_04300, GP975_01190, GP979_02000, GQA06_03945, GQM04_10570, GQM13_25205, GQM17_19920, GQM21_11235, GQN34_23270, GQW07_21545, GRO95_20810, GRW05_09065, GRW24_04750, GRW56_00300, GRW57_03535, GSM54_23325, GSY44_20005, GUC01_21185, GUI33_20050, H0O53_20825, H0O72_19420, H0P11_20920, H5Z35_004789, H6Y26_003164, HCQ42_003948, HEP30_018640, HEP34_004770, HHH44_004535, HI055_004140, HIE29_005187, HJY54_004753, HKA49_005035, HLQ92_22595, HLV18_23335, HLX92_10070, HLZ50_22175, HMV95_19610, HMW38_23110, HV109_02185, HV146_21675, HV209_14710, HVV39_09270, HVW04_17530, HVW43_18665, HVY77_02170, HVZ29_19100, HVZ30_02050, HVZ71_02355, HX136_02190, I6H00_20920, I6H02_11995, IFB95_004806, IUD45_003540, J0541_004397, J4S20_004802, J5U05_004087, J7T50_001453, JFD_01884, JFD_03713, JNP96_25310, KQ660_11775, NCTC10082_02839, NCTC10089_00502, NCTC10090_03527, NCTC10418_00690, NCTC10429_01095, NCTC10487_00445, NCTC10764_03863, NCTC10767_01516, NCTC10865_00661, NCTC10974_00551, NCTC11126_02771, NCTC11127_03223, NCTC11181_02738, NCTC11341_01889, NCTC12950_00479, NCTC13127_00786, NCTC4450_02475, NCTC7922_04042, NCTC7927_00551, NCTC7928_02516, NCTC8009_01511, NCTC8179_05942, NCTC8333_00489, NCTC8450_04937, NCTC8500_00335, NCTC8621_00508, NCTC8622_00507, NCTC8959_03261, NCTC8960_03070, NCTC8985_04982, NCTC9001_04254, NCTC9044_01356, NCTC9045_00566, NCTC9050_03611, NCTC9071_00483, NCTC9073_00380, NCTC9075_00724, NCTC9077_00604, NCTC9081_00918, NCTC9111_00867, NCTC9117_00756, NCTC9702_00537, NCTC9706_02737, NCTC9775_04003, NCTC9777_01891, NEP60_26295, OS110_25120, RG28_21615, SAMEA3472044_01162, SAMEA3472056_05411, SAMEA3752557_01952, SAMEA3753106_04634, TUM18780_04360, WR15_19610
Production host: Escherichia coli (E. coli) / References: UniProt: C3SR32
#32: Protein 50S ribosomal protein L31 / Large ribosomal subunit protein bL31-A


Mass: 7887.117 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmE, b3936, JW3907 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7M9
#33: Protein 50S ribosomal protein L32


Mass: 6463.445 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpmF, rpmF_1, A5U30_002830, A8502_000661, A9X72_15435, ACN81_09130, ACU57_04225, AM464_23480, AW118_12110, AWP47_13180, B6R15_001792, B6R31_003138, BANRA_01624, BCB93_002043, BER14_10210, BF481_ ...Gene: rpmF, rpmF_1, A5U30_002830, A8502_000661, A9X72_15435, ACN81_09130, ACU57_04225, AM464_23480, AW118_12110, AWP47_13180, B6R15_001792, B6R31_003138, BANRA_01624, BCB93_002043, BER14_10210, BF481_002131, BG944_003607, BGM66_002729, BJI68_20640, BJJ90_15780, BK292_02505, BK383_03640, BKL28_002219, BTB68_003153, BTQ06_08080, BvCmsKKP061_02173, BvCmsSIP010_04163, BXT93_21825, C0P57_001747, C1Q91_003167, C2121_002257, C2M16_23600, C2R31_002348, C3F40_06530, C9E67_18185, C9Z68_07340, CA593_23040, CF22_003088, CG692_05970, CG704_04105, CG831_001542, CIG67_21935, CQ986_001084, CR538_15635, CR539_10135, CR628_002785, CTR35_002374, CV83915_00494, CY655_06595, D1912_08350, D3C88_19355, D3G36_11860, D4M65_04795, D4N09_11015, D9D43_11825, D9E49_23135, D9J61_04035, DD762_03625, DIV22_29035, DNQ45_10015, DNX30_12140, DQ87_002042, DS732_10505, DTL43_16270, DU321_22840, E4K51_18340, E5H86_12535, E6D34_10935, EAI46_03975, EC95NR1_05485, ECs1467, EEP55_000822, EIA08_02925, EIZ93_13150, EN85_002840, EPS76_00770, EPS97_06370, EWK56_20060, ExPECSC038_03490, F7F11_10320, F7N46_02450, F9413_18150, F9461_13585, F9B07_16900, FEJ01_12565, FIJ20_07300, FJQ40_00940, FKO60_17370, FOI11_007500, FOI11_14470, FPI65_06540, FPS11_20235, FTV92_08325, FTV93_08485, FV293_19565, FVB16_04695, FWK02_15570, FZN31_14620, FZU14_20010, G3V95_08050, G3W53_15755, G4A38_16050, G4A47_15410, G5603_00600, G8C01_10030, G9448_05130, GAI89_13280, GAJ12_13930, GGB84_000483, GJ11_06555, GKF66_05180, GNW61_10640, GOP25_17405, GP711_16475, GP954_12815, GP965_04045, GP975_03585, GP979_13150, GQA06_08430, GQM04_02110, GQM13_14340, GQM21_06935, GQN34_10245, GQW07_08880, GRO95_10420, GRW05_02410, GRW24_27585, GRW56_06550, GRW57_07595, GTP92_19120, GUC01_01680, H0O72_16850, HCQ42_001914, HEP30_011955, HEP34_001075, HHH44_003019, HI055_001485, HIE29_000052, HJQ60_005092, HLX92_03690, HLZ50_01830, HMV95_16370, HV109_14610, HV146_09250, HV209_29060, HVV39_00410, HVW04_05590, HVW43_06430, HVY77_15765, HVZ29_08715, HX086_07035, HX136_15650, I0E88_003516, I6H00_08540, I6H02_24530, IFB95_003696, IUC12_000121, J0541_003169, J5U05_002959, JNP96_10710, KV259_001392, KV317_003230, KV371_000953, KV406_001102, KV449_001342, KV455_003227, KV463_001049, KV469_000542, KV499_000679, KV500_001199, NCTC10082_00641, NCTC10089_03005, NCTC10090_00680, NCTC10418_04509, NCTC10764_02647, NCTC10767_05044, NCTC10865_03684, NCTC10974_03416, NCTC11126_00919, NCTC11181_00164, NCTC11341_04894, NCTC12950_03296, NCTC13148_05706, NCTC4450_04127, NCTC7922_00634, NCTC7927_03385, NCTC7928_00455, NCTC8009_05657, NCTC8179_02874, NCTC8333_03590, NCTC8621_03238, NCTC8622_05813, NCTC8959_00826, NCTC8960_00763, NCTC8985_01747, NCTC9044_04397, NCTC9045_03550, NCTC9073_01975, NCTC9075_04022, NCTC9077_03763, NCTC9081_03511, NCTC9117_03837, NCTC9706_00412, NCTC9962_06984, NEP60_22810, O5851_21660, RG28_16890, SAMEA3752557_01555, TUM18780_25170, WR15_23855
Production host: Escherichia coli (E. coli) / References: UniProt: C3TE02
#34: Protein 50S ribosomal protein L33


Mass: 6388.631 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpmG, rpmG_1, A5U30_004136, A6592_21955, A8499_000792, A8502_002345, A9X72_00400, AC065_17670, ACN81_07125, ACU57_07205, AM464_10960, APX88_07835, AW118_10740, AWP47_15985, B6R12_001909, B6R15_ ...Gene: rpmG, rpmG_1, A5U30_004136, A6592_21955, A8499_000792, A8502_002345, A9X72_00400, AC065_17670, ACN81_07125, ACU57_07205, AM464_10960, APX88_07835, AW118_10740, AWP47_15985, B6R12_001909, B6R15_003224, B6R17_003336, B6R31_002865, B6R48_003283, B6R87_003156, BANRA_04677, BB540_10150, BER14_16165, BF481_000671, BG944_002239, BGM66_003973, BHS81_21690, BJI68_21710, BJJ90_00390, BK292_09690, BK383_09650, BKL28_001549, BMT50_10565, BMT91_13845, BR158_003843, BTB68_002398, BTQ06_17850, BUO55_001749, BvCmsKKP061_03496, BvCmsSIP010_04504, BXT93_06220, BZL69_22230, C0P57_003843, C1Q91_001907, C2121_003322, C2M16_17175, C2R31_004081, C3F40_17705, C5N07_22255, C9E67_00890, C9Z68_15420, CA593_07900, CCS08_06865, CCU84_004237, CDL36_16035, CF22_002026, CG692_01875, CG704_10010, CG831_000991, CIG67_03795, CQ986_000108, CQB02_22645, CR538_00630, CR539_23800, CR628_001528, CRM83_20955, CTR35_001077, CV83915_02464, CWS33_14490, CX938_000998, CY655_23100, D3C88_33140, D3G36_09395, D4M65_14910, D4N09_11500, D6T60_21010, D9D43_20690, D9E34_16170, D9E49_10195, D9H94_14645, D9J03_19890, D9J61_14005, DD762_15060, DIV22_25130, DNQ45_17840, DNX30_21910, DS732_26145, DTL43_17805, DTL90_19180, DTM45_13255, DU321_24275, E0I52_14235, E4K51_19990, E5H86_15640, E6D34_14175, EA239_11595, EB534_RS18180, EC95NR1_03037, ECs4511, EIA08_13065, EIMP300_11190, EIZ93_22910, EN85_002943, EPS76_16410, EPS97_14120, ERS085406_02634, ERS139208_03027, EWK56_19235, ExPECSC038_00886, F3P10_10725, F7F11_22995, F7N46_06895, F9413_20155, F9461_04500, F9B07_09850, FA868_19515, FEJ01_05435, FFF58_21805, FGG80_07720, FHD44_20765, FHQ97_14415, FIJ20_14735, FJQ40_22750, FKO60_20795, FN993_001905, FOI11_017545, FOI11_05635, FPI65_22380, FPS11_15665, FTV92_18865, FTV93_19030, FV293_17885, FVB16_08575, FWK02_20790, FZN31_00850, FZU14_11310, G3V95_06555, G3W53_09755, G4A38_07645, G4A47_07035, G5603_14155, G9448_15395, GAI89_21025, GAJ12_25750, GAJ26_19905, GGB84_002816, GIB53_07680, GJ11_23260, GKF52_07040, GKF66_06090, GKF89_01305, GNW61_15010, GOP14_002925, GOP25_22240, GP711_14020, GP965_18420, GP975_06785, GQM04_18880, GQM13_19245, GQM17_16535, GQM21_20675, GQN34_13365, GQW07_04785, GRO95_05820, GRW05_17070, GRW24_04500, GRW56_13140, GRW57_14295, GSM54_05345, GSY44_19835, GUC01_19125, GUI33_04785, H0O53_07375, H0O72_08715, H0P11_12255, H5Z35_003529, H6Y26_001161, HCQ42_003479, HEP30_004320, HEP34_000581, HHH44_001955, HI055_001939, HIE29_002315, HJQ60_004388, HJY54_003834, HKA49_003561, HLQ92_16260, HLV18_18020, HLX92_21165, HLZ50_07765, HMV95_17960, HMW38_12345, HV109_00345, HV146_00330, HV209_24710, HVV39_10960, HVW04_15855, HVW43_16870, HVY77_00425, HVZ29_00305, HVZ30_00460, HVZ71_00385, HX136_00340, I6H00_19205, I6H02_13660, IFB95_001325, IUD45_003655, J0541_001519, J4S20_003173, J5U05_003670, J7T50_004317, JFD_02865, JFD_02951, JNP96_26980, KQ660_13480, NCTC10082_02483, NCTC10089_00164, NCTC10090_03179, NCTC10418_00180, NCTC10429_00616, NCTC10487_00103, NCTC10764_03481, NCTC10767_01151, NCTC10865_00201, NCTC10974_00127, NCTC11112_01599, NCTC11126_01709, NCTC11127_03609, NCTC11181_02394, NCTC11341_02337, NCTC12950_00092, NCTC13127_00380, NCTC4450_02132, NCTC7922_04520, NCTC7927_00189, NCTC7928_02970, NCTC8009_00971, NCTC8179_05536, NCTC8333_00121, NCTC8450_04550, NCTC8500_05792, NCTC8621_00171, NCTC8959_03599, NCTC8960_02723, NCTC8985_04527, NCTC9001_03782, NCTC9044_01835, NCTC9045_00169, NCTC9050_03266, NCTC9071_00140, NCTC9073_06389, NCTC9075_00237, NCTC9077_00137, NCTC9081_00502, NCTC9111_00516, NCTC9117_00296, NCTC9702_00106, NCTC9706_02375, NCTC9775_03637, NCTC9777_01519, NCTC9962_01072, NEP60_12970, OS110_19615, RG28_23350, SAMEA3472044_00655, SAMEA3472056_04704, SAMEA3752557_01144, SAMEA3753106_03495, TUM18780_00760, WR15_15810
Production host: Escherichia coli (E. coli) / References: UniProt: C3SME7
#35: Protein/peptide 50S ribosomal protein L34


Mass: 5397.463 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmH / Production host: Escherichia coli (E. coli) / References: UniProt: B7MGC4
#36: Protein 50S ribosomal protein L35 / Large ribosomal subunit protein bL35 / Ribosomal protein A


Mass: 7313.032 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmI, b1717, JW1707 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7Q1
#37: Protein/peptide 50S ribosomal protein L36


Mass: 4377.390 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmJ / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0E2L017

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Large ribosomal subunit protein ... , 15 types, 15 molecules DEFGJKLNOPRSVWY

#9: Protein Large ribosomal subunit protein uL4 / 50S ribosomal protein L4


Mass: 22121.566 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplD, eryA, b3319, JW3281 / Production host: Escherichia coli (E. coli) / References: UniProt: P60723
#10: Protein Large ribosomal subunit protein uL5 / 50S ribosomal protein L5


Mass: 20333.611 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplE, b3308, JW3270 / Production host: Escherichia coli (E. coli) / References: UniProt: P62399
#11: Protein Large ribosomal subunit protein uL6 / 50S ribosomal protein L6


Mass: 18932.791 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplF, b3305, JW3267 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AG55
#12: Protein Large ribosomal subunit protein bL9 / 50S ribosomal protein L9


Mass: 15789.020 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplI, b4203, JW4161 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7R1
#15: Protein Large ribosomal subunit protein uL13 / 50S ribosomal protein L13


Mass: 16050.606 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplM, b3231, JW3200 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AA10
#16: Protein Large ribosomal subunit protein uL14 / 50S ribosomal protein L14


Mass: 13565.067 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplN, b3310, JW3272 / Production host: Escherichia coli (E. coli) / References: UniProt: P0ADY3
#17: Protein Large ribosomal subunit protein uL15 / 50S ribosomal protein L15


Mass: 15008.471 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplO, b3301, JW3263 / Production host: Escherichia coli (E. coli) / References: UniProt: P02413
#19: Protein Large ribosomal subunit protein bL17 / 50S ribosomal protein L17


Mass: 14393.657 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplQ, b3294, JW3256 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AG44
#20: Protein Large ribosomal subunit protein uL18 / 50S ribosomal protein L18


Mass: 12794.668 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplR, b3304, JW3266 / Production host: Escherichia coli (E. coli) / References: UniProt: P0C018
#21: Protein Large ribosomal subunit protein bL19


Mass: 13159.278 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplS / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7K8
#23: Protein Large ribosomal subunit protein bL21 / 50S ribosomal protein L21


Mass: 11586.374 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplU, UTI89_C3620 / Production host: Escherichia coli (E. coli) / References: UniProt: Q1R6F0
#24: Protein Large ribosomal subunit protein uL22 / 50S ribosomal protein L22


Mass: 12253.359 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplV, UTI89_C3767 / Production host: Escherichia coli (E. coli) / References: UniProt: Q1R610
#27: Protein Large ribosomal subunit protein bL25


Mass: 10713.465 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplY / Production host: Escherichia coli (E. coli) / References: UniProt: P68919
#28: Protein Large ribosomal subunit protein bL27


Mass: 9146.540 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmA / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7M0
#30: Protein Large ribosomal subunit protein uL29


Mass: 7286.464 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpmC, A5U30_004546, A8502_004029, ACN81_25490, ACU57_20990, AW118_24870, AWP47_12675, B6R15_004075, B6R31_004974, BANRA_01943, BANRA_04322, BE932_15505, BER14_25160, BG944_001127, BGM66_003599, ...Gene: rpmC, A5U30_004546, A8502_004029, ACN81_25490, ACU57_20990, AW118_24870, AWP47_12675, B6R15_004075, B6R31_004974, BANRA_01943, BANRA_04322, BE932_15505, BER14_25160, BG944_001127, BGM66_003599, BGZ_02596, BGZ_04119, BJI68_08705, BK292_20475, BK383_24675, BKL28_004549, BTB68_004362, BTQ06_10660, BvCmsKKP061_03024, BvCmsSIP010_02679, BXT93_05505, C0P57_002662, C1Q91_004910, C2121_004168, C2M16_25435, C2R31_004627, C3F40_19750, C9Z68_22605, CF22_004855, CG704_20900, CG831_003643, CIG67_10705, CTR35_003624, CV83915_02786, D3G36_23080, D4M65_20625, D4N09_20225, D9D43_22580, D9E49_05210, D9J61_16705, DD762_23600, DIV22_15205, DNQ45_03810, DNX30_25425, DS732_24315, DTL43_20945, DU321_12055, E4K51_21545, E5H86_24470, E6D34_21705, EAI46_07530, ECs4177, EIA08_24010, EIMP300_17750, EIZ93_12415, EN85_004355, EPS76_07160, EPS97_20040, EWK56_25050, ExPECSC038_03646, F7F11_22670, F7N46_23955, F9413_21180, F9461_25600, F9B07_24630, FGAF848_25910, FIJ20_21140, FJQ40_18360, FKO60_25690, FOI11_019205, FOI11_03975, FORC44_0486, FPI65_20360, FPS11_25375, FTV93_20845, FVB16_04925, FWK02_06325, FZU14_21875, G3V95_19900, G3W53_21010, G4A38_21565, G4A47_20835, G5603_24640, GAI89_24775, GAJ12_24665, GKF66_21600, GNW61_16685, GOP25_22760, GP711_23360, GP954_00890, GP965_04350, GP975_01240, GP979_01950, GQA06_03895, GQE86_20135, GQM04_10620, GQM13_25255, GQM21_11285, GQN34_23320, GQW07_21495, GRC73_21695, GRO95_20760, GRW05_09115, GRW24_04700, GRW56_00350, GRW57_03485, GUC01_21235, H0O72_19470, HEP30_018690, HEP34_004760, HHH44_004525, HI055_004150, HIE29_005197, HJQ60_005001, HLX92_10020, HLZ50_22125, HMV95_19660, HV109_02135, HV209_14660, HVV39_09320, HVW04_17480, HVW43_18615, HVY77_02120, I6H00_20870, I6H02_12045, IFB95_004816, J0541_004407, J5U05_004077, JNP96_25360, KV499_004411, NCTC10082_02829, NCTC10089_00492, NCTC10418_00681, NCTC10429_01084, NCTC10764_03853, NCTC10767_01506, NCTC10865_00649, NCTC10974_00539, NCTC11126_02760, NCTC11181_02728, NCTC11341_01899, NCTC7922_04052, NCTC7927_00541, NCTC7928_02527, NCTC8009_01501, NCTC8179_05932, NCTC8333_00479, NCTC8500_00324, NCTC8621_00498, NCTC8622_00516, NCTC8959_03271, NCTC8960_03060, NCTC8985_04972, NCTC9001_04244, NCTC9044_01368, NCTC9045_00555, NCTC9075_00714, NCTC9077_00594, NCTC9081_00907, NCTC9117_00746, NCTC9702_00527, NCTC9706_02727, NCTC9962_06787, NEP60_26345, OGM49_22385, QDW62_02140, RZR61_12945, SAMEA3472112_00683, SAMEA3752557_01962, WR15_19660
Production host: Escherichia coli (E. coli) / References: UniProt: C3SQY2

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30S ribosomal protein ... , 11 types, 11 molecules ghiklpruwyz

#38: Protein 30S ribosomal protein S2


Mass: 26781.670 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsB / Production host: Escherichia coli (E. coli) / References: UniProt: C3TPN2
#39: Protein 30S ribosomal protein S3


Mass: 26031.316 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsC / Production host: Escherichia coli (E. coli) / References: UniProt: B7MCS9
#40: Protein 30S ribosomal protein S4


Mass: 23514.199 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsD, ramA, b3296, JW3258 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7V8
#42: Protein 30S ribosomal protein S6 / Small ribosomal subunit protein bS6


Mass: 15727.512 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsF, b4200, JW4158 / Production host: Escherichia coli (E. coli) / References: UniProt: P02358
#43: Protein 30S ribosomal protein S7 / Small ribosomal subunit protein uS7


Mass: 20055.156 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsG, b3341, JW3303 / Production host: Escherichia coli (E. coli) / References: UniProt: P02359
#47: Protein 30S ribosomal protein S11


Mass: 13870.975 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsK / Production host: Escherichia coli (E. coli) / References: UniProt: B7MCR3
#49: Protein 30S ribosomal protein S13


Mass: 13128.467 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsM, G711_03878 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A7U9IV78
#52: Protein 30S ribosomal protein S16


Mass: 9207.572 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsP / Production host: Escherichia coli (E. coli) / References: UniProt: C3SYP2
#54: Protein 30S ribosomal protein S18


Mass: 9005.472 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsR / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0E2KXL3
#56: Protein 30S ribosomal protein S20


Mass: 9708.464 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsT / Production host: Escherichia coli (E. coli) / References: UniProt: C3TRH7
#57: Protein 30S ribosomal protein S21


Mass: 8524.039 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsU / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0E2L2J1

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Small ribosomal subunit protein ... , 9 types, 9 molecules jmnoqstvx

#41: Protein Small ribosomal subunit protein uS5 / 30S ribosomal protein S5


Mass: 17629.398 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli 0.1197 (bacteria) / Gene: rpsE, spc, b3303, JW3265 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7W1
#44: Protein Small ribosomal subunit protein uS8


Mass: 14146.557 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsH / Production host: Escherichia coli (E. coli) / References: UniProt: C3SR12
#45: Protein Small ribosomal subunit protein uS9


Mass: 14886.270 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsI / Production host: Escherichia coli (E. coli) / References: UniProt: C3SRY2
#46: Protein Small ribosomal subunit protein uS10


Mass: 11755.597 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsJ / Production host: Escherichia coli (E. coli) / References: UniProt: C3SQT7
#48: Protein Small ribosomal subunit protein uS12


Mass: 13814.249 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpsL, rpsL_2, A5U30_003889, A6592_18175, A8499_001308, A8502_001035, A9X72_02055, AC065_16140, ACN81_28395, ACU57_16335, AM464_09350, APX88_20135, AW118_19390, AWP47_12580, B6R12_000406, B6R15_ ...Gene: rpsL, rpsL_2, A5U30_003889, A6592_18175, A8499_001308, A8502_001035, A9X72_02055, AC065_16140, ACN81_28395, ACU57_16335, AM464_09350, APX88_20135, AW118_19390, AWP47_12580, B6R12_000406, B6R15_000411, B6R17_004092, B6R31_002238, B6R48_002865, B6R87_002647, BANRA_04338, BB540_24940, BER14_21580, BF481_000994, BG944_001143, BGM66_002197, BHS81_20025, BJI68_15120, BJJ90_01995, BK292_07945, BK383_21385, BKL28_001878, BMT50_12160, BMT91_03340, BR158_000836, BTB68_000004, BTQ06_10750, BUO55_003823, BvCmsKKP061_02719, BvCmsSIP010_02074, BXT93_17220, BZL69_05785, C0P57_000744, C1Q91_001774, C2121_001894, C2M16_10980, C2R31_002848, C3F40_19595, C5N07_11865, C9E67_02665, C9Z68_19360, CA593_09530, CCS08_20045, CCU84_000778, CDL36_09745, CF22_001801, CG692_18330, CG704_07645, CG831_001292, CIG67_21255, CQ986_000431, CQB02_21500, CR538_02285, CR539_21960, CR628_001376, CRM83_22785, CTR35_001824, CV83915_02769, CWS33_10820, CX938_000715, CY655_21380, D1912_26245, D3C88_25690, D3G36_06655, D4M65_08955, D4N09_12780, D6T60_05835, D9D43_04015, D9E34_05370, D9E49_05360, D9H94_02360, D9J03_02800, D9J61_04455, DD762_11810, DIV22_04600, DNQ45_18710, DNX30_18200, DS732_24475, DTL43_13765, DTL90_06910, DTM45_15580, DU321_12135, E0I52_09925, E4K51_10545, E5H86_09220, E6D34_04010, EA239_12250, EAI46_06760, EB534_RS01835, EC95NR1_02719, ECs4193, EIA08_12060, EIZ93_01385, EN85_000596, EPS76_12695, EPS97_04830, ERS085406_01915, ERS139208_04106, EWK56_16220, ExPECSC038_01207, F3P10_03820, F7F11_11880, F7N46_14475, F9413_23615, F9461_02860, F9B07_12795, FA868_17430, FEJ01_10185, FFF58_13835, FGG80_13730, FHD44_12480, FHQ97_05960, FIJ20_20680, FJQ40_03220, FKO60_13770, FN993_002408, FOI11_019055, FOI11_04125, FPI65_20520, FPS11_03565, FTV92_20575, FTV93_20685, FV293_08660, FVB16_09155, FWK02_07820, FZN31_02500, FZU14_04005, G3V95_05795, G3W53_10630, G4A38_16715, G4A47_18305, G5603_11210, G9448_16940, GAI89_06495, GAJ12_06765, GAJ26_22145, GGB84_001627, GIB53_18845, GJ11_21590, GKF52_05665, GKF66_04705, GKF89_12265, GNW61_19825, GOP14_000555, GOP25_12270, GP711_17590, GP965_17670, GQM04_08870, GQM13_20845, GQM17_11035, GQM21_13045, GQN34_11870, GQW07_20360, GRO95_19555, GRW05_23630, GRW24_12145, GRW56_12155, GRW57_13500, GSM54_01330, GSY44_04760, GUC01_14585, GUI33_18070, H0O53_20260, H0O72_13185, H0P11_14900, H5Z35_001968, H6Y26_003204, HCQ42_003768, HEP30_005280, HEP34_002415, HHH44_000548, HI055_002196, HIE29_002006, HJQ60_002804, HJY54_002830, HKA49_001073, HLQ92_04845, HLV18_11670, HLX92_09940, HLZ50_06780, HMV95_07635, HMW38_08385, HV109_01985, HV146_21805, HV209_14580, HVV39_09470, HVW04_17400, HVW43_18535, HVY77_01970, HVZ29_19230, HVZ30_01920, HVZ71_02225, HX136_01990, I6H00_20790, I6H02_12125, IFB95_001027, IUD45_003566, J0541_002092, J4S20_001155, J5U05_000731, J7T50_001493, JFD_01925, JFD_03753, JNP96_25510, KQ660_11905, NCTC10082_02799, NCTC10089_00476, NCTC10090_03501, NCTC10487_00418, NCTC10764_03836, NCTC10767_01481, NCTC10974_00472, NCTC11112_01085, NCTC11126_02723, NCTC11127_03263, NCTC11181_02712, NCTC11341_01920, NCTC12950_00439, NCTC13127_00758, NCTC13148_03489, NCTC4450_02449, NCTC7922_04088, NCTC7927_00509, NCTC8009_01480, NCTC8179_05915, NCTC8333_00463, NCTC8450_04911, NCTC8621_00480, NCTC8959_03287, NCTC8960_03044, NCTC8985_04955, NCTC9001_04208, NCTC9044_01386, NCTC9045_00521, NCTC9050_03585, NCTC9071_00443, NCTC9081_00877, NCTC9111_00841, NCTC9702_00511, NCTC9706_02614, NCTC9775_03976, NCTC9777_01848, NCTC9962_06745, NEP60_15855, OS110_10240, RG28_21750, SAMEA3472044_01122, SAMEA3472056_01947, SAMEA3752557_01992, SAMEA3753106_01938, TUM18780_04100, WR15_02645
Production host: Escherichia coli (E. coli) / References: UniProt: C3SQR7
#50: Protein Small ribosomal subunit protein uS14


Mass: 11606.560 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsN, NCTC9073_00385 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A2X1PQW4
#51: Protein Small ribosomal subunit protein uS15


Mass: 10290.816 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsO / Production host: Escherichia coli (E. coli) / References: UniProt: C3SSQ7
#53: Protein Small ribosomal subunit protein uS17


Mass: 9724.491 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsQ / Production host: Escherichia coli (E. coli) / References: UniProt: C3SQY7
#55: Protein Small ribosomal subunit protein uS19


Mass: 10455.355 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsS / Production host: Escherichia coli (E. coli) / References: UniProt: C3SQW2

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Non-polymers , 3 types, 443 molecules

#58: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 439 / Source method: obtained synthetically / Formula: Mg
#59: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#60: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: H2O

-
Details

Has ligand of interestN
Has protein modificationY

-
Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

-
Sample preparation

Component
IDNameTypeEntity IDParent-IDSource
1Damaged 70S with PrfHRIBOSOME#1-#570MULTIPLE SOURCES
250SCOMPLEX#1, #3, #7-#371NATURAL
330SCOMPLEX1NATURAL
4tRNAORGANELLE OR CELLULAR COMPONENT#41RECOMBINANT
5Putative peptide chain release factor homologORGANELLE OR CELLULAR COMPONENT#61RECOMBINANT
Source (natural)Organism: Escherichia coli (E. coli)
Source (recombinant)Organism: Escherichia coli (E. coli)
Buffer solutionpH: 7.4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid type: Quantifoil R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1000 nm
Image recordingElectron dose: 30 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: FEI FALCON III (4k x 4k)

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Processing

EM software
IDNameCategory
4CTFFINDCTF correction
10cryoSPARCinitial Euler assignment
11cryoSPARCfinal Euler assignment
12cryoSPARCclassification
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 2.78 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 104212 / Symmetry type: POINT

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