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- EMDB-48105: Lgl2 bound to the aPKCiota-Par6B complex in its ADP-bound form -

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Entry
Database: EMDB / ID: EMD-48105
TitleLgl2 bound to the aPKCiota-Par6B complex in its ADP-bound form
Map data
Sample
  • Complex: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)
    • Complex: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)
      • Protein or peptide: Partitioning defective 6 homolog beta
    • Complex: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)
      • Protein or peptide: LLGL scribble cell polarity complex component 2
      • Protein or peptide: Protein kinase C iota type
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: MAGNESIUM ION
KeywordsCell Polarity / Kinase / Complex / LIPID BINDING PROTEIN
Function / homology
Function and homology information


establishment of spindle orientation / establishment or maintenance of polarity of embryonic epithelium / Golgi vesicle budding / diacylglycerol-dependent, calcium-independent serine/threonine kinase activity / PAR polarity complex / Tight junction interactions / regulation of establishment or maintenance of cell polarity / protein kinase C / establishment of apical/basal cell polarity / diacylglycerol-dependent serine/threonine kinase activity ...establishment of spindle orientation / establishment or maintenance of polarity of embryonic epithelium / Golgi vesicle budding / diacylglycerol-dependent, calcium-independent serine/threonine kinase activity / PAR polarity complex / Tight junction interactions / regulation of establishment or maintenance of cell polarity / protein kinase C / establishment of apical/basal cell polarity / diacylglycerol-dependent serine/threonine kinase activity / L-leucine transport / myosin II binding / negative regulation of glial cell apoptotic process / regulation of Notch signaling pathway / eye photoreceptor cell development / branching involved in labyrinthine layer morphogenesis / Schmidt-Lanterman incisure / Golgi to plasma membrane transport / establishment or maintenance of epithelial cell apical/basal polarity / cellular response to chemical stress / membrane organization / cell-cell junction organization / tight junction / cortical actin cytoskeleton organization / protein targeting to membrane / labyrinthine layer blood vessel development / cortical actin cytoskeleton / positive regulation of Notch signaling pathway / establishment of cell polarity / cell leading edge / exocytosis / brush border / positive regulation of glial cell proliferation / positive regulation of endothelial cell apoptotic process / bicellular tight junction / regulation of postsynaptic membrane neurotransmitter receptor levels / p75NTR recruits signalling complexes / intercellular bridge / vesicle-mediated transport / cytoskeleton organization / secretion / response to interleukin-1 / GTPase activator activity / actin filament organization / post-embryonic development / protein localization to plasma membrane / positive regulation of D-glucose import / adherens junction / positive regulation of protein localization to plasma membrane / PDZ domain binding / positive regulation of NF-kappaB transcription factor activity / positive regulation of neuron projection development / phospholipid binding / Pre-NOTCH Transcription and Translation / Schaffer collateral - CA1 synapse / multicellular organism growth / cellular response to insulin stimulus / KEAP1-NFE2L2 pathway / cell migration / microtubule cytoskeleton / negative regulation of neuron apoptotic process / protein phosphorylation / protein kinase activity / endosome / intracellular signal transduction / cilium / apical plasma membrane / Golgi membrane / cell division / protein serine kinase activity / intracellular membrane-bounded organelle / protein serine/threonine kinase activity / negative regulation of apoptotic process / glutamatergic synapse / extracellular exosome / zinc ion binding / nucleoplasm / ATP binding / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Lethal(2) giant larvae protein / Lethal giant larvae homologue 2 / LLGL2 / Atypical protein kinase C iota type, catalytic domain / Protein kinase C / Protein kinase C, PB1 domain / PB1 domain / PB1 domain / PB1 domain / : ...Lethal(2) giant larvae protein / Lethal giant larvae homologue 2 / LLGL2 / Atypical protein kinase C iota type, catalytic domain / Protein kinase C / Protein kinase C, PB1 domain / PB1 domain / PB1 domain / PB1 domain / : / PB1 domain profile. / Protein kinase, C-terminal / Protein kinase C terminal domain / Diacylglycerol/phorbol-ester binding / Phorbol esters/diacylglycerol binding domain (C1 domain) / Zinc finger phorbol-ester/DAG-type signature. / Zinc finger phorbol-ester/DAG-type profile. / Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains) / Protein kinase C-like, phorbol ester/diacylglycerol-binding domain / C1-like domain superfamily / Extension to Ser/Thr-type protein kinases / AGC-kinase, C-terminal / AGC-kinase C-terminal domain profile. / WD domain, G-beta repeat / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / WD40/YVTN repeat-like-containing domain superfamily / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
Protein kinase C iota type / LLGL scribble cell polarity complex component 2
Similarity search - Component
Biological speciesMus musculus (house mouse) / Homo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.33 Å
AuthorsAlmagor L / Weis WI
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Commun Biol / Year: 2025
Title: Polarity protein Par6 facilitates the processive phosphorylation of Lgl via a dynamic interaction with aPKC.
Authors: Lior Almagor / William I Weis /
Abstract: Polarity along an apical-basal axis is essential for epithelial cell shape and function. The atypical protein Kinase-C (aPKC) and its regulatory partner Par6 form a complex that is essential for ...Polarity along an apical-basal axis is essential for epithelial cell shape and function. The atypical protein Kinase-C (aPKC) and its regulatory partner Par6 form a complex that is essential for polarization, a primary function of which is to phosphorylate the Lethal giant larvae (Lgl) protein to prevent it from binding to the apical membrane (thereby facilitating its basolateral localization). Par6 binds Lgl directly and is essential for this process, but its mechanism was obscure. Here, we utilize cryo-EM and various biochemical techniques to characterize the interaction of Lgl2 with the aPKCι/Par6 complex and to study the roles of Par6 in promoting Lgl2 phosphorylation. We find that Par6 proteins stabilize a ternary Lgl2/aPKCι/Par6 complex that involves a unique multi-surface interaction of Lgl2 with both aPKCι and Par6. Importantly, we find Par6b induces processive phosphorylation that results in a multi-phosphorylated Lgl2 after a single interaction with the aPKCι/Par6b complex. This is enabled by a Par6b/Lgl2 interaction that maintains contact of Lgl2 with the kinase throughout its distinct nucleotide-binding states. Our results reveal the mechanistic basis for the efficient regulation of Lgl's membrane binding by aPKC/Par6 and provide invaluable structural data for further understanding the mechanisms of this polarity complex.
History
DepositionNov 28, 2024-
Header (metadata) releaseJul 2, 2025-
Map releaseJul 2, 2025-
UpdateJul 16, 2025-
Current statusJul 16, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_48105.png

Downloads & links

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Map

FileDownload / File: emd_48105.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
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AxesZ (Sec.)Y (Row.)X (Col.)
0.56 Å/pix.
x 512 pix.
= 284.416 Å		0.56 Å/pix.
x 512 pix.
= 284.416 Å		0.56 Å/pix.
x 512 pix.
= 284.416 Å

Surface

Projections

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.5555 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.08
Minimum - Maximum-0.53188884 - 0.72792614
Average (Standard dev.)-0.0000010964269 (±0.015326738)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 284.416 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_48105_half_map_1.map
Projections & Slices
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Histogram (log scale)

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Half map: #1

Fileemd_48105_half_map_2.map
Projections & Slices
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Histogram (log scale)

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Sample components

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Entire : A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)

EntireName: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)
Components
  • Complex: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)
    • Complex: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)
      • Protein or peptide: Partitioning defective 6 homolog beta
    • Complex: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)
      • Protein or peptide: LLGL scribble cell polarity complex component 2
      • Protein or peptide: Protein kinase C iota type
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: MAGNESIUM ION

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Supramolecule #1: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)

SupramoleculeName: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3

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Supramolecule #2: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)

SupramoleculeName: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)
type: complex / ID: 2 / Parent: 1 / Macromolecule list: #3
Source (natural)Organism: Mus musculus (house mouse)

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Supramolecule #3: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)

SupramoleculeName: A ternary complex of Lgl2 with aPKC iota and Par6B (ADP-bound)
type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1-#2
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: LLGL scribble cell polarity complex component 2

MacromoleculeName: LLGL scribble cell polarity complex component 2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 109.362188 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MRERLKRDLF QFNKTVEHGF PHQPSALGYS PSLRILAIGT RSGAIKLYGA PGVEFMGLHQ ENNAVTQIHL LPGQCQLVTL LDDNSLHLW SLKVKGGASE LQEDESFTLR GPPGAAPSAT QITVVLPHSS CELLYLGTES GNVFVVQLPA FRALEDRTIS S DAVLQRLP ...String:
MRERLKRDLF QFNKTVEHGF PHQPSALGYS PSLRILAIGT RSGAIKLYGA PGVEFMGLHQ ENNAVTQIHL LPGQCQLVTL LDDNSLHLW SLKVKGGASE LQEDESFTLR GPPGAAPSAT QITVVLPHSS CELLYLGTES GNVFVVQLPA FRALEDRTIS S DAVLQRLP EEARHRRVFE MVEALQEHPR DPNQILIGYS RGLVVIWDLQ GSRVLYHFLS SQQLENIWWQ RDGRLLVSCH SD GSYCQWP VSSEAQQPEP LRSLVPYGPF PCKAITRILW LTTRQGLPFT IFQGGMPRAS YGDRHCISVI HDGQQTAFDF TSR VIGFTV LTEADPAATF DDPYALVVLA EEELVVIDLQ TAGWPPVQLP YLASLHCSAI TCSHHVSNIP LKLWERIIAA GSRQ NAHFS TMEWPIDGGT SLTPAPPQRD LLLTGHEDGT VRFWDASGVC LRLLYKLSTV RVFLTDTDPN ENFSAQGEDE WPPLR KVGS FDPYSDDPRL GIQKIFLCKY SGYLAVAGTA GQVLVLELND EAAEQAVEQV EADLLQDQEG YRWKGHERLA ARSGPV RFE PGFQPFVLVQ CQPPAVVTSL ALHSEWRLVA FGTSHGFGLF DHQQRRQVFV KCTLHPSDQL ALEGPLSRVK SLKKSLR QS FRRMRRSRVS SRKRHPAGPP GEAQEGSAKA ERPGLQNMEL APVQRKIEAR SAEDSFTGFV RTLYFADTYL KDSSRHCP S LWAGTNGGTI YAFSLRVPPA ERRMDEPVRA EQAKEIQLMH RAPVVGILVL DGHSVPLPEP LEVAHDLSKS PDMQGSHQL LVVSEEQFKV FTLPKVSAKL KLKLTALEGS RVRRVSVAHF GSRRAEDYGE HHLAVLTNLG DIQVVSLPLL KPQVRYSCIR REDVSGIAS CVFTKYGQGF YLISPSEFER FSLSTKWLVE PRCLVDSAET KNHRPGNGAG PKKAPSRARN SGTQSDGEEK Q PGLVMERE FTTSASENLY FQ

UniProtKB: LLGL scribble cell polarity complex component 2

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Macromolecule #2: Protein kinase C iota type

MacromoleculeName: Protein kinase C iota type / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: protein kinase C
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 68.512258 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MPTQRDSSTM SHTVAGGGSG DHSHQVRVKA YYRGDIMITH FEPSISFEGL CNEVRDMCSF DNEQLFTMKW IDEEGDPCTV SSQLELEEA FRLYELNKDS ELLIHVFPCV PERPGMPCPG EDKSIYRRGA RRWRKLYCAN GHTFQAKRFN RRAHCAICTD R IWGLGRQG ...String:
MPTQRDSSTM SHTVAGGGSG DHSHQVRVKA YYRGDIMITH FEPSISFEGL CNEVRDMCSF DNEQLFTMKW IDEEGDPCTV SSQLELEEA FRLYELNKDS ELLIHVFPCV PERPGMPCPG EDKSIYRRGA RRWRKLYCAN GHTFQAKRFN RRAHCAICTD R IWGLGRQG YKCINCKLLV HKKCHKLVTI ECGRHSLPQE PVMPMDQSSM HSDHAQTVIP YNPSSHESLD QVGEEKEAMN TR ESGKASS SLGLQDFDLL RVIGRGSYAK VLLVRLKKTD RIYAMKVVKK ELVNDDEDID WVQTEKHVFE QASNHPFLVG LHS CFQTES RLFFVIEYVN GGDLMFHMQR QRKLPEEHAR FYSAEISLAL NYLHERGIIY RDLKLDNVLL DSEGHIKLTD YGMC KEGLR PGDTTS(TPO)FCG TPNYIAPEIL RGEDYGFSVD WWALGVLMFE MMAGRSPFDI VGSSDNPDQN TEDYLFQVIL E KQIRIPRS LSVKAASVLK SFLNKDPKER LGCHPQTGFA DIQGHPFFRN VDWDMMEQKQ VVPPFKPNIS GEFGLDNFDS QF TNEPVQL (TPO)PDDDDIVRK IDQSEFEGFE YINPLLMSAE ECV

UniProtKB: Protein kinase C iota type

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Macromolecule #3: Partitioning defective 6 homolog beta

MacromoleculeName: Partitioning defective 6 homolog beta / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 42.472445 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MNRGHRHGAS SGCLGTMEVK SKFGAEFRRF SLERSKPGKF EEFYGLLQHV HKIPNVDVLV GYADIHGDLP PINNDDNYHK AVSTANPLL RIFIQKKEEA DYSAFGTDTL IRKKNMLSNV LRPDNHRKKP HIVISMPQDF RPVSSIIDVD ILPETHRRVR L CKYGTEKP ...String:
MNRGHRHGAS SGCLGTMEVK SKFGAEFRRF SLERSKPGKF EEFYGLLQHV HKIPNVDVLV GYADIHGDLP PINNDDNYHK AVSTANPLL RIFIQKKEEA DYSAFGTDTL IRKKNMLSNV LRPDNHRKKP HIVISMPQDF RPVSSIIDVD ILPETHRRVR L CKYGTEKP LGFYIRDGSS VRVTPHGLEK VPGIFISRLV PGGLAQSTGL LAVNDEVLEV NGIEVSGKSL DQVTDMMIAN SR NLIITVR PANQRNNVVR NSRTSGSSSQ STDNSLLGFP QQVEASFEPE DQDSDEDDII IEDSGEPQQI PKATPAQSLE SLT QIELSF ESGQNGFSPP QDTSLVPVPG SLDTELESRA PDQKLLEEDG TIITLEFTTA SENLYFQ

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Macromolecule #4: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 4 / Number of copies: 1 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

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Macromolecule #5: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 5 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.2 mg/mL
BufferpH: 8
GridModel: Quantifoil R1.2/1.3 / Material: GOLD / Support film - Material: GOLD / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 295 K / Instrument: FEI VITROBOT MARK IV
Details20 mM Tris 200 mM NaCl 1 mM DTT 10 mM MgCl2 1 mM ADP 10 uM ZnCl2 0.05% n-octyl-beta-D-glucoside

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Electron microscopy

MicroscopeTFS KRIOS
DetailsData collected at both 25 and 40 degrees tilt
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 65.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Calibrated defocus max: 3.5 µm / Calibrated defocus min: 1.3 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.3 µm / Nominal magnification: 81000
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER / Details: Ab initio reconstruction by cryoSPARC
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.33 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 787439
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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