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基本情報
登録情報 | ![]() | |||||||||
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タイトル | M. smegmatis unmethylated 70S ribosome structure | |||||||||
![]() | Unsharpened postprocessed map of the unmethylated M. smegmatis 70S ribosome | |||||||||
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![]() | 70S ribosome / unmethylated ribosome / ribonucleoprotein complex / protein synthesis / RIBOSOME | |||||||||
機能・相同性 | ![]() large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding ...large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / zinc ion binding / metal ion binding / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.24 Å | |||||||||
![]() | Nandi S / Conn GL | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Distant ribose 2'-O-methylation of 23S rRNA helix 69 pre-orders the capreomycin drug binding pocket at the ribosome subunit interface. 著者: Suparno Nandi / Debayan Dey / Pooja Srinivas / Christine M Dunham / Graeme L Conn / ![]() 要旨: Loss of ribosomal RNA (rRNA) modifications incorporated by the intrinsic methyltransferase TlyA results in reduced sensitivity to tuberactinomycin antibiotics such as capreomycin. However, how rRNA ...Loss of ribosomal RNA (rRNA) modifications incorporated by the intrinsic methyltransferase TlyA results in reduced sensitivity to tuberactinomycin antibiotics such as capreomycin. However, how rRNA methylation alters drug binding, particularly at the distant but functionally more important site in 23S rRNA helix 69 (H69), is currently unknown. We determined high-resolution cryo-electron microscopy structures of the Mycolicibacterium smegmatis 70S ribosome with or without the two ribose 2'-O-methyl modifications incorporated by TlyA. In the unmodified ribosome, the tip of H69 adopts a more compact conformation, positioning two key nucleotides (A2137 and C2138) such that interactions with capreomycin would be lost and the binding pocket partially occluded. Methylation of 23S rRNA nucleotide C2144 promotes conformational changes that result in a more favorable positioning of C2138 and adoption of a more open conformation to enable capreomycin binding. Molecular dynamics simulations and H69 RNA helical analyses additionally reveal specific propagation of these changes from the site of modification to the H69 tip, allosterically reconfiguring the capreomycin binding site. Methylation of h44 also results in structural rearrangements at the H69-h44 interface to support maintenance of these changes that favor antibiotic binding. This work thus reveals the effect and regulation of distant rRNA methylation on ribosome-targeting antibiotic binding. | |||||||||
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マップデータ | ![]() | 225.1 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 100.2 KB 100.2 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 14.1 KB | 表示 | ![]() |
画像 | ![]() | 59.3 KB | ||
Filedesc metadata | ![]() | 15.1 KB | ||
その他 | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | 177.7 MB 217.5 MB 217.3 MB 159.1 MB 178.4 MB 160.5 MB 193 MB 176.5 MB 160.4 MB 220.8 MB 220.2 MB 195.7 MB 195.7 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 1.1 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1.1 MB | 表示 | |
XML形式データ | ![]() | 21.6 KB | 表示 | |
CIF形式データ | ![]() | 28.7 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 9e0nMC ![]() 9e0pC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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注釈 | Unsharpened postprocessed map of the unmethylated M. smegmatis 70S ribosome | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.069 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
+追加マップ: Half-map from the multibody refinement of the unmethylated...
+追加マップ: DeepEMhancer-sharpened postprocessed map from the multibody refinement of...
+追加マップ: DeepEMhancer-sharpened postprocessed map from the multibody refinement of...
+追加マップ: Multibody refined map of the unmethylated M. smegmatis 30S subunit
+追加マップ: Half-map from the multibody refinement of the unmethylated...
+追加マップ: Half-map from the multibody refinement of the unmethylated...
+追加マップ: Refine3D map of the unmethylated M. smegmatis 70S ribosome
+追加マップ: Multibody refined map of the unmethylated M. smegmatis
+追加マップ: Half-map from the multibody refinement of the unmethylated...
+追加マップ: PHENIX-sharpened postprocessed map from the multibody refinement of...
+追加マップ: PHENIX-sharpened postprocessed map from the multibody refinement of...
+ハーフマップ: Half-map from the 3D refinement of the unmethylated...
+ハーフマップ: Half-map from the 3D refinement of the unmethylated...
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試料の構成要素
+全体 : M. smegmatis unmethylated 70S ribosome
+超分子 #1: M. smegmatis unmethylated 70S ribosome
+分子 #1: Large ribosomal subunit protein uL30
+分子 #2: Large ribosomal subunit protein bL31
+分子 #3: Large ribosomal subunit protein bL32
+分子 #4: Large ribosomal subunit protein bL33A
+分子 #5: Large ribosomal subunit protein bL34
+分子 #6: Large ribosomal subunit protein bL35
+分子 #7: 50S ribosomal protein L36
+分子 #8: 50S ribosomal protein bL37
+分子 #11: Large ribosomal subunit protein uL2
+分子 #12: Large ribosomal subunit protein uL3
+分子 #13: Large ribosomal subunit protein uL4
+分子 #14: Large ribosomal subunit protein uL5
+分子 #15: Large ribosomal subunit protein uL6
+分子 #16: 50S ribosomal protein L9
+分子 #17: Large ribosomal subunit protein uL10
+分子 #18: Large ribosomal subunit protein uL11
+分子 #19: Large ribosomal subunit protein uL13
+分子 #20: Large ribosomal subunit protein uL14
+分子 #21: Large ribosomal subunit protein uL15
+分子 #22: Large ribosomal subunit protein uL16
+分子 #23: Large ribosomal subunit protein bL17
+分子 #24: Large ribosomal subunit protein uL18
+分子 #25: Large ribosomal subunit protein bL19
+分子 #26: Large ribosomal subunit protein bL20
+分子 #27: Large ribosomal subunit protein bL21
+分子 #28: Large ribosomal subunit protein uL22
+分子 #29: Large ribosomal subunit protein uL23
+分子 #30: Large ribosomal subunit protein uL24
+分子 #31: Large ribosomal subunit protein bL25
+分子 #32: Large ribosomal subunit protein bL27
+分子 #33: Large ribosomal subunit protein bL28
+分子 #34: Large ribosomal subunit protein uL29
+分子 #36: Small ribosomal subunit protein uS2
+分子 #37: Small ribosomal subunit protein uS3
+分子 #38: Small ribosomal subunit protein uS4
+分子 #39: Small ribosomal subunit protein uS5
+分子 #40: Small ribosomal subunit protein bS6
+分子 #41: Small ribosomal subunit protein uS7
+分子 #42: Small ribosomal subunit protein uS8
+分子 #43: Small ribosomal subunit protein uS9
+分子 #44: Small ribosomal subunit protein uS10
+分子 #45: Small ribosomal subunit protein uS11
+分子 #46: Small ribosomal subunit protein uS12
+分子 #47: Small ribosomal subunit protein uS13
+分子 #48: Small ribosomal subunit protein uS14B
+分子 #49: Small ribosomal subunit protein uS15
+分子 #50: Small ribosomal subunit protein bS16
+分子 #51: Small ribosomal subunit protein uS17
+分子 #52: Small ribosomal subunit protein bS18B
+分子 #53: Small ribosomal subunit protein uS19
+分子 #54: Small ribosomal subunit protein bS20
+分子 #55: Conserved domain protein
+分子 #9: 23S rRNA
+分子 #10: 5S rRNA
+分子 #35: 16S rRNA
+分子 #56: MAGNESIUM ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.6 |
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グリッド | モデル: Quantifoil R1.2/1.3 / 材質: COPPER / メッシュ: 300 / 支持フィルム - 材質: CARBON / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 15 sec. / 前処理 - 雰囲気: OTHER |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277.15 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | TFS KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) デジタル化 - サイズ - 横: 5760 pixel / デジタル化 - サイズ - 縦: 4092 pixel / 実像数: 5060 / 平均露光時間: 2.0 sec. / 平均電子線量: 52.51 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 2.1 µm / 最小 デフォーカス(公称値): 0.6 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |