EMDB-47365: M. smegmatis methylated 70S ribosome structure

Basic information

Entry

Database: EMDB / ID: EMD-47365

• Title: M. smegmatis methylated 70S ribosome structure
• Map data: Unsharpened postprocessed map of the methylated M. smegmatis 70S ribosome

Sample

• Complex: M. smegmatis methylated 70S ribosome
  • Protein or peptide: x 52 types
  • RNA: x 3 types
• Ligand: x 1 types

• Keywords: 70S ribosome / unmethylated ribosome / ribonucleoprotein complex / protein synthesis / RIBOSOME

Function / homology

Function:

large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / zinc ion binding / metal ion binding / cytosol / cytoplasm

Domain/homology:

Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein L10 / : / : / Ribosomal protein S14, type Z / Ribosomal protein L11, bacterial-type / Ribosomal protein L31 type A / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / : / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / : / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / : / Ribosomal protein S19, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein L5, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35

Component:

Small ribosomal subunit protein bS6 / Large ribosomal subunit protein bL9 / Conserved domain protein / Large ribosomal subunit protein bL33A / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL10 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Uncharacterized protein / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein bS18B / Large ribosomal subunit protein bL34

• Biological species: Mycolicibacterium smegmatis (bacteria)
• Method: single particle reconstruction / cryo EM / Resolution: 3.17 Å
• Authors: Nandi S / Conn GL

Funding support

United States, 1 items

Organization	Grant number	Country
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)	R01-AI088025	United States

• Citation: Journal: Nucleic Acids Res / Year: 2025; Title: Distant ribose 2'-O-methylation of 23S rRNA helix 69 pre-orders the capreomycin drug binding pocket at the ribosome subunit interface.; Authors: Suparno Nandi / Debayan Dey / Pooja Srinivas / Christine M Dunham / Graeme L Conn / ; Abstract: Loss of ribosomal RNA (rRNA) modifications incorporated by the intrinsic methyltransferase TlyA results in reduced sensitivity to tuberactinomycin antibiotics such as capreomycin. However, how rRNA methylation alters drug binding, particularly at the distant but functionally more important site in 23S rRNA helix 69 (H69), is currently unknown. We determined high-resolution cryo-electron microscopy structures of the Mycolicibacterium smegmatis 70S ribosome with or without the two ribose 2'-O-methyl modifications incorporated by TlyA. In the unmodified ribosome, the tip of H69 adopts a more compact conformation, positioning two key nucleotides (A2137 and C2138) such that interactions with capreomycin would be lost and the binding pocket partially occluded. Methylation of 23S rRNA nucleotide C2144 promotes conformational changes that result in a more favorable positioning of C2138 and adoption of a more open conformation to enable capreomycin binding. Molecular dynamics simulations and H69 RNA helical analyses additionally reveal specific propagation of these changes from the site of modification to the H69 tip, allosterically reconfiguring the capreomycin binding site. Methylation of h44 also results in structural rearrangements at the H69-h44 interface to support maintenance of these changes that favor antibiotic binding. This work thus reveals the effect and regulation of distant rRNA methylation on ribosome-targeting antibiotic binding.

History

Deposition	Oct 18, 2024	-
Header (metadata) release	Jul 16, 2025	-
Map release	Jul 16, 2025	-
Update	Jul 16, 2025	-
Current status	Jul 16, 2025	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_47365.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Unsharpened postprocessed map of the methylated M. smegmatis 70S ribosome
• Voxel size: X=Y=Z: 1.069 Å

Density

Contour Level	By AUTHOR: 0.014
Minimum - Maximum	-0.0119024655 - 0.078544855
Average (Standard dev.)	-0.00012419699 (±0.0051891976)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 427.6 Å α=β=γ: 90.0 °

Supplemental data

Additional map: Half-map obtained from the multibody refinement of the...

• File: emd_47365_additional_1.map
• Annotation: Half-map obtained from the multibody refinement of the methylated M. smegmatis 30S subunit

Additional map: Unsharpened postprocessed map from the multibody refinement of...

• File: emd_47365_additional_10.map
• Annotation: Unsharpened postprocessed map from the multibody refinement of the methylated M. smegmatis 30S subunit

Additional map: PHENIX-sharpened postprocessed map from the multibody refinement of...

• File: emd_47365_additional_11.map
• Annotation: PHENIX-sharpened postprocessed map from the multibody refinement of the methylated M. smegmatis 30S subunit

Additional map: DeepEMhancer-sharpened half-map obtained from the multibody refinement of...

• File: emd_47365_additional_12.map
• Annotation: DeepEMhancer-sharpened half-map obtained from the multibody refinement of the methylated M. smegmatis 50S subunit

Additional map: DeepEMhancer-sharpened half-map obtained from the multibody refinement of...

• File: emd_47365_additional_13.map
• Annotation: DeepEMhancer-sharpened half-map obtained from the multibody refinement of the methylated M. smegmatis 30S subunit

Additional map: Half-map obtained from the multibody refinement of the...

• File: emd_47365_additional_2.map
• Annotation: Half-map obtained from the multibody refinement of the methylated M. smegmatis 30S subunit

Additional map: Half-map obtained from the multibody refinement of the...

• File: emd_47365_additional_3.map
• Annotation: Half-map obtained from the multibody refinement of the methylated M. smegmatis 50S subunit

Additional map: Half-map obtained from the multibody refinement of the...

• File: emd_47365_additional_4.map
• Annotation: Half-map obtained from the multibody refinement of the methylated M. smegmatis 50S subunit

Additional map: Multibody refined map of the methylated M. smegmatis 30S subunit

• File: emd_47365_additional_5.map
• Annotation: Multibody refined map of the methylated M. smegmatis 30S subunit

Additional map: Multibody refined map of the methylated M. smegmatis 50S subunit

• File: emd_47365_additional_6.map
• Annotation: Multibody refined map of the methylated M. smegmatis 50S subunit

Additional map: 3D refinement map of the methylated M. smegmatis 70S ribosome

• File: emd_47365_additional_7.map
• Annotation: 3D refinement map of the methylated M. smegmatis 70S ribosome

Additional map: PHENIX-sharpened postprocessed map from the multibody refinement of...

• File: emd_47365_additional_8.map
• Annotation: PHENIX-sharpened postprocessed map from the multibody refinement of the methylated M. smegmatis 50S subunit

Additional map: Unsharpened postprocessed map from the multibody refinement of...

• File: emd_47365_additional_9.map
• Annotation: Unsharpened postprocessed map from the multibody refinement of the methylated M. smegmatis 50S subunit

Half map: Half-map obtained from 3D refinement of the methylated...

• File: emd_47365_half_map_1.map
• Annotation: Half-map obtained from 3D refinement of the methylated M. smegmatis 70S ribosome

Half map: Half-map obtained from 3D refinement of the methylated...

• File: emd_47365_half_map_2.map
• Annotation: Half-map obtained from 3D refinement of the methylated M. smegmatis 70S ribosome

Sample components

Entire : M. smegmatis methylated 70S ribosome

• Entire: Name: M. smegmatis methylated 70S ribosome

Components

• Complex: M. smegmatis methylated 70S ribosome
  • Protein or peptide: Large ribosomal subunit protein uL30
  • Protein or peptide: Large ribosomal subunit protein bL31
  • Protein or peptide: Large ribosomal subunit protein bL32
  • Protein or peptide: Large ribosomal subunit protein bL33A
  • Protein or peptide: Large ribosomal subunit protein bL34
  • Protein or peptide: Large ribosomal subunit protein bL35
  • Protein or peptide: 50S ribosomal protein L36
  • Protein or peptide: 50S ribosomal protein bL37
  • RNA: 23S rRNA
  • RNA: 5S rRNA
  • Protein or peptide: Large ribosomal subunit protein uL2
  • Protein or peptide: Large ribosomal subunit protein uL3
  • Protein or peptide: Large ribosomal subunit protein uL4
  • Protein or peptide: Large ribosomal subunit protein uL5
  • Protein or peptide: Large ribosomal subunit protein uL6
  • Protein or peptide: 50S ribosomal protein L9
  • Protein or peptide: Large ribosomal subunit protein uL10
  • Protein or peptide: Large ribosomal subunit protein uL11
  • Protein or peptide: Large ribosomal subunit protein uL13
  • Protein or peptide: Large ribosomal subunit protein uL14
  • Protein or peptide: Large ribosomal subunit protein uL15
  • Protein or peptide: Large ribosomal subunit protein uL16
  • Protein or peptide: Large ribosomal subunit protein bL17
  • Protein or peptide: Large ribosomal subunit protein uL18
  • Protein or peptide: Large ribosomal subunit protein bL19
  • Protein or peptide: Large ribosomal subunit protein bL20
  • Protein or peptide: Large ribosomal subunit protein bL21
  • Protein or peptide: Large ribosomal subunit protein uL22
  • Protein or peptide: Large ribosomal subunit protein uL23
  • Protein or peptide: Large ribosomal subunit protein uL24
  • Protein or peptide: Large ribosomal subunit protein bL25
  • Protein or peptide: Large ribosomal subunit protein bL27
  • Protein or peptide: Large ribosomal subunit protein bL28
  • Protein or peptide: Large ribosomal subunit protein uL29
  • RNA: 16S rRNA
  • Protein or peptide: Small ribosomal subunit protein uS2
  • Protein or peptide: Small ribosomal subunit protein uS3
  • Protein or peptide: Small ribosomal subunit protein uS4
  • Protein or peptide: Small ribosomal subunit protein uS5
  • Protein or peptide: Small ribosomal subunit protein bS6
  • Protein or peptide: Small ribosomal subunit protein uS7
  • Protein or peptide: Small ribosomal subunit protein uS8
  • Protein or peptide: Small ribosomal subunit protein uS9
  • Protein or peptide: Small ribosomal subunit protein uS10
  • Protein or peptide: Small ribosomal subunit protein uS11
  • Protein or peptide: Small ribosomal subunit protein uS12
  • Protein or peptide: Small ribosomal subunit protein uS13
  • Protein or peptide: Small ribosomal subunit protein uS14B
  • Protein or peptide: Small ribosomal subunit protein uS15
  • Protein or peptide: Small ribosomal subunit protein bS16
  • Protein or peptide: Small ribosomal subunit protein uS17
  • Protein or peptide: Small ribosomal subunit protein bS18B
  • Protein or peptide: Small ribosomal subunit protein uS19
  • Protein or peptide: Small ribosomal subunit protein bS20
  • Protein or peptide: Conserved domain protein
• Ligand: MAGNESIUM ION

Supramolecule #1: M. smegmatis methylated 70S ribosome

• Supramolecule: Name: M. smegmatis methylated 70S ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#55
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 2.3 MDa

Macromolecule #1: Large ribosomal subunit protein uL30

• Macromolecule: Name: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 7.022147 KDa

Sequence

String:

MAELKITQVR STIGARWKQR ESLRTLGLKK IRQSVVREDN AQTRGLINTV HHLVEVEEVG K

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #2: Large ribosomal subunit protein bL31

• Macromolecule: Name: Large ribosomal subunit protein bL31 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 8.312485 KDa

Sequence

String:

MKTGIHPEYV DTTVQCGCGH SFTTRSTKQS GTIVVEVCSQ CHPFYTGKQK ILDSGGRVAR FEKRYGKRNK AAADK

UniProtKB: Large ribosomal subunit protein bL31

Macromolecule #3: Large ribosomal subunit protein bL32

• Macromolecule: Name: Large ribosomal subunit protein bL32 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 6.467719 KDa

Sequence

String:

MAVPKRRMSR ANTRSRRAQW KAEAPGLVTV SVAGQQRKVP RRLLKAARLG LVDLDKR

UniProtKB: Large ribosomal subunit protein bL32

Macromolecule #4: Large ribosomal subunit protein bL33A

• Macromolecule: Name: Large ribosomal subunit protein bL33A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 6.468501 KDa

Sequence

String:

MASSTDVRPK ITLACEVCKH RNYITKKNRR NDPDRLEIKK FCPNCGTHQP HKESR

UniProtKB: Large ribosomal subunit protein bL33A

Macromolecule #5: Large ribosomal subunit protein bL34

• Macromolecule: Name: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 5.52258 KDa

Sequence

String:

MAKGKRTFQP NNRRRARVHG FRLRMRTRAG RAIVANRRSK GRRALTA

UniProtKB: Large ribosomal subunit protein bL34

Macromolecule #6: Large ribosomal subunit protein bL35

• Macromolecule: Name: Large ribosomal subunit protein bL35 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 7.298505 KDa

Sequence

String:

MPKAKTHSGA SKRFRRTGTG KIVRQKANRR HLLEHKPTKR TRRLDGRTTV SAADNSRINK LLNG

UniProtKB: Large ribosomal subunit protein bL35

Macromolecule #7: 50S ribosomal protein L36

• Macromolecule: Name: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 4.341277 KDa

Sequence

String:

MKVNPSVKPI CDKCRVIRRH GRVMVICSDP RHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

Macromolecule #8: 50S ribosomal protein bL37

• Macromolecule: Name: 50S ribosomal protein bL37 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 2.841375 KDa

Sequence

String:

MAKRGRKKRD RKHSKANHGK RPNA

UniProtKB: Uncharacterized protein

Macromolecule #11: Large ribosomal subunit protein uL2

• Macromolecule: Name: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 30.425945 KDa

Sequence

String:

MGIRKYKPTT PGRRGASVSD FAEITRSTPE KSLVRPLHGK GGRNAHGRIT TRHKGGGHKR AYRVIDFRRH DKDGVNAKVA HIEYDPNRT ANIALLHYLD GEKRYIIAPQ GLKQGDVIES GANADIKPGN NLPLRNIPAG TVIHAVELRP GGGAKLARSA G VSIQLLGK EGTYAALRMP SGEIRRVDVR CRATVGEVGN AEQSNINWGK AGRMRWKGKR PTVRGVVMNP VDHPHGGGEG KT SGGRHPV SPWGKPEGRT RKPNKPSDKL IVRRRRTGKK R

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #12: Large ribosomal subunit protein uL3

• Macromolecule: Name: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 23.011393 KDa

Sequence

String:

MARKGILGTK LGMTQVFDEN NKVVPVTVVK AGPNVVTRIR TTERDGYSAV QLAYGEISPR KVIKPVAGQF AAAGVNPRRH VAELRLDDE AAVAEYEVGQ ELTAEIFSDG AYVDVTGTSK GKGFAGTMKR HGFRGQGAAH GAQAVHRRPG SIGGCATPGR V FKGTRMSG RMGNDRVTTQ NLKVHKVDAE NGVLLIKGAI PGRNGGLVVV RSAIKRGEK

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #13: Large ribosomal subunit protein uL4

• Macromolecule: Name: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 22.920949 KDa

Sequence

String:

MTLKVDVKTP AGKTDGSVEL PAELFDVEPN IALMHQVVTA QLAAKRQGTH STKTRGEVSG GGKKPYRQKG TGRARQGSTR APQFTGGGT VHGPKPRDYS QRTPKKMIAA ALRGALSDRA RNDRIHAVTE LVEGQTPSTK SAKTFLGTLT ENKKVLVVIG R TDEVGAKS VRNLPGVHVI SPDQLNTYDV LNADDVVFSV EALNAYISAN SKEGASV

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #14: Large ribosomal subunit protein uL5

• Macromolecule: Name: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 21.152328 KDa

Sequence

String:

MTTTEKALPR LKQRYREEIR EALQQEFNYA NVMQIPGVVK VVVNMGVGDA ARDAKLINGA INDLALITGQ KPEVRRARKS IAQFKLREG MPIGARVTLR GDRMWEFLDR LISIALPRIR DFRGLSPKQF DGTGNYTFGL NEQSMFHEID VDSIDRPRGM D ITVVTTAT NDAEGRALLR ALGFPFKEN

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #15: Large ribosomal subunit protein uL6

• Macromolecule: Name: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 19.483361 KDa

Sequence

String:

MSRIGKQPVP VPSGVDVTIN GQNLSVKGPK GTLTLDVAEP ISVSRAEDGA IVVTRPDDER RSRSLHGLSR TLIANLVTGV TEGYTQKME IFGVGYRVQL KGQNLEFALG YSHPVLIEAP EGITFAVESP TKFSVSGIDK QKVGQISAVI RRLRRPDPYK G KGVRYEGE QIRRKVGKTG K

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #16: 50S ribosomal protein L9

• Macromolecule: Name: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 15.949253 KDa

Sequence

String:

MKLILTAEVE HLGAAGDTVE VKDGYGRNYL LPRGLAIVAS RGAERQAEEI RRARESKVIR DIEHANELKT ALEGLGDVTL SVNAAGDTG KLFGSVTAAD VVNAIKKAGG PNLDKRTVQL AKAHIKSVGT HPVTVKLHTG VEAKVSLNVV AQ

UniProtKB: Large ribosomal subunit protein bL9

Macromolecule #17: Large ribosomal subunit protein uL10

• Macromolecule: Name: Large ribosomal subunit protein uL10 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 17.964658 KDa

Sequence

String:

MAKADKATAV ADIAEQFKAS TATVVTEYRG LTVANLAELR RALGDSATYT VAKNTLVKRA ASEAGIEGLD ELFAGPTAIA FVKGEAVDA AKAIKKFAKD NKALVIKGGY MDGKALSVAD VEKIADLESR EVLLAKLAGA MKGNLSKAAG LFNAPASQVA R LAAALQEK KAGEEA

UniProtKB: Large ribosomal subunit protein uL10

Macromolecule #18: Large ribosomal subunit protein uL11

• Macromolecule: Name: Large ribosomal subunit protein uL11 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 15.023374 KDa

Sequence

String:

MAPKKKVAGL IKLQIQAGQA NPAPPVGPAL GQHGVNIMEF CKAYNAATES QRGNVIPVEI TVYEDRSFTF ALKTPPAAKL LLKAAGVQK GSGEPHKTKV AKVTWDQVRE IAETKKADLN ANDIDAAAKI IAGTARSMGI TVE

UniProtKB: Large ribosomal subunit protein uL11

Macromolecule #19: Large ribosomal subunit protein uL13

• Macromolecule: Name: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 16.146689 KDa

Sequence

String:

MPTYTPKAGD TTRSWYVIDA SDVVLGRLAS AAATLLRGKH KPTFTPNVDG GDFVIVINAD KIAVSGDKLT KKFAYRHSGY PGGLRKRTI GELLEKHPTR VVENAIIGML PHNKLGRQIQ KKLKVYAGPD HPHAAQQPIP FEIKQVAQ

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #20: Large ribosomal subunit protein uL14

• Macromolecule: Name: Large ribosomal subunit protein uL14 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 13.400625 KDa

Sequence

String:

MIQQESRLKV ADNTGAKEIL CIRVLGGSSR RYAGIGDVIV ATVKDAIPGG NVKRGDVVKA VVVRTVKERR RADGSYIKFD ENAAVIIKN DNDPRGTRIF GPVGRELREK KFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #21: Large ribosomal subunit protein uL15

• Macromolecule: Name: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 15.602244 KDa

Sequence

String:

MSVIKLHDLK PAPGEKKAKT RVGRGEGSKG KTAGRGTKGT KARKNVPVMF EGGQMPIHMR LPKLKGFKNR FRTEYQVVNV GDINKAFPQ GGTVGVDELV AKGLVRKNSL VKVLGDGKLT VKVDVTANKF SGSAREAITA AGGSATEL

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #22: Large ribosomal subunit protein uL16

• Macromolecule: Name: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 15.77424 KDa

Sequence

String:

MLIPRKVKHR KQHHPEQRGI ASGGTSVSFG DYGIQALEHA YITNRQIESA RIAINRHIKR GGKVWINIFP DRPLTKKPAE TRMGSGKGS PEWWVANVKP GRVLFELSYP DEKTARDALT RAIHKLPIKA RIVTREEQF

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #23: Large ribosomal subunit protein bL17

• Macromolecule: Name: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 19.210605 KDa

Sequence

String:

MPKPTKGPRL GGSSSHQSAL LANLATSLFE HGRIKTTEPK ARALRPYAEK LITHAKKGAL HNRREVMKKI RDKDVVHTLF AEIGPFYAD RNGGYTRIIK VENRKGDNAP MAVIELVREK TVTDEANRAR RAAASQAKAD ERADEKADEK AEETVEETTE A PAEESTEA AAEETV

UniProtKB: Large ribosomal subunit protein bL17

Macromolecule #24: Large ribosomal subunit protein uL18

• Macromolecule: Name: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 13.711728 KDa

Sequence

String:

MAHKPVGQNI SEVRRNARLR RHARLRKKVA GTAEVPRLVV NRSARHIHVQ LVNDLNGTTL AAASSIEADV RAIDGDKKAH SVRVGQLIA ERAKAAGVET VVFDRGGYTY GGRIAALADA AREAGLKF

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #25: Large ribosomal subunit protein bL19

• Macromolecule: Name: Large ribosomal subunit protein bL19 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 12.892806 KDa

Sequence

String:

MNTLDFVDQA SLRDDIPTFS PGDTVNVHVK VIEGSKERIQ VFKGVVIRRQ GGGISETFTV RKESYGVGVE RTFPVHSPNI DHIDVLTRG DVRRAKLYYL RELRGKKAKI KEKR

UniProtKB: Large ribosomal subunit protein bL19

Macromolecule #26: Large ribosomal subunit protein bL20

• Macromolecule: Name: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 14.494693 KDa

Sequence

String:

MARVKRALNA QKKRRTVLKA SKGYRGQRSR LYRKAKEQQL HSLTYAYRDR RARKGEFRKL WISRINAAAR ANDITYNRLI QGLKAAGVE VDRKNLAELA VSDPAAFTAL VDVARAALPE DVNAPSGEAA

UniProtKB: Large ribosomal subunit protein bL20

Macromolecule #27: Large ribosomal subunit protein bL21

• Macromolecule: Name: Large ribosomal subunit protein bL21 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 11.055894 KDa

Sequence

String:

MATYAIVKTG GKQYKVAAGD VVKVEKLDSE PGASVSLPVA LVVDGANVTS KADDLAKVAV TAEVLEHTKG PKIRIHKFKN KTGYHKRQG HRQQLTVLKV TGIK

UniProtKB: Large ribosomal subunit protein bL21

Macromolecule #28: Large ribosomal subunit protein uL22

• Macromolecule: Name: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 16.3536 KDa

Sequence

String:

MSTVTEFPSA TAKARYVRVS ATKARRVIDL VRGKSVEEAL DILRWAPQAA SEPVAKVIAS AAANAQNNEG LDPSTLVVAT VYADEGPTA KRIRPRAQGR AFRIRKRTSH ITVIVESRPP KQKGASAASA RSRRAQGSKA AATKKSAETK EGSE

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #29: Large ribosomal subunit protein uL23

• Macromolecule: Name: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 11.047836 KDa

Sequence

String:

MATITDPRDI ILAPVISEKS YGLIEDNVYT FVVHPDSNKT QIKIAIEKIF DVKVDSVNTA NRQGKRKRTR TGFGKRKSTK RAIVKLAAG SKPIDLFGAP A

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #30: Large ribosomal subunit protein uL24

• Macromolecule: Name: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 11.228946 KDa

Sequence

String:

MKVHKGDTVL VISGKDKGAK GKVLVAYPDR NKVLVEGVNR IKKHTAVSAN ERGASSGGIV TQEAPIHVSN VMVVDSDGKP TRVGYRIDD ETGKKVRIAK TNGKDI

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #31: Large ribosomal subunit protein bL25

• Macromolecule: Name: Large ribosomal subunit protein bL25 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 22.571078 KDa

Sequence

String:

MAKTANIPNK LTANVRTRTG KGASRQARRD GKVPAVLYGH GTDPQHLELN ARDFAAVLRS HGTNAILTLD IEGTEQLALT KALDVHPIR RNIQHADLLV VQRGEKVTVE VTVLVEGDAT PGTLVTQDAN TIEIEAEALS IPEQLTVSVE GVEAGTQITA G QISLPEGV NLISDPELLV VNVVEAPSAE ALEEEGAGET GAAEAPAEEA PAEEPAE

UniProtKB: Large ribosomal subunit protein bL25

Macromolecule #32: Large ribosomal subunit protein bL27

• Macromolecule: Name: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 9.249582 KDa

Sequence

String:

MAHKKGASSS RNGRDSAAQR LGVKRFGGQV VKAGEILVRQ RGTHFHPGVN VGRGGDDTLF ALAPGAVEFG AKRGRKTVNI VPVARPEA

UniProtKB: Large ribosomal subunit protein bL27

Macromolecule #33: Large ribosomal subunit protein bL28

• Macromolecule: Name: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 6.891016 KDa

Sequence

String:

MAAVCDICGK GPGFGKSVSH SHRRTSRRWN PNIQPVRAVT RPGGNKQRIN ACTSCIKAGK VSRA

UniProtKB: Large ribosomal subunit protein bL28

Macromolecule #34: Large ribosomal subunit protein uL29

• Macromolecule: Name: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 8.790895 KDa

Sequence

String:

MAVGTTPGEL RELTDDELKD KLRESKEELF NLRFQMATGQ LSNNRRLRTV RQEIARVYTV LRERELGLAS GPAGEES

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #36: Small ribosomal subunit protein uS2

• Macromolecule: Name: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 30.14523 KDa

Sequence

String:

MAVVTMKQLL DSGAHFGHQT RRWNPKMKRF IFTDRNGIYI IDLQQTLTYI DKAYEFVKET VAHGGTVLFV GTKKQAQESI AEEATRVGM PYVNQRWLGG MLTNFSTVHK RLQRLKELEA MEQTGGFEGR TKKEILMLTR EKNKLERSLG GIRDMQKVPS A VWVVDTNK EHIAVGEARK LGIPVIAILD TNCDPDVVDY PIPGNDDAIR SAALLTKVIA SAVAEGLQAR AGQGSGEKPA EG AEPLAEW EQELLAGATA GAADASAEGA AAPESSTDAS

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #37: Small ribosomal subunit protein uS3

• Macromolecule: Name: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 30.191227 KDa

Sequence

String:

MGQKINPHGF RLGITTEWKS RWYADKQYKD YVKEDVAIRK LLATGLERAG IADVEIERTR DRVRVDIHTA RPGIVIGRRG TEADRIRAD LEKLTGKQVQ LNILEVKNPE SQAQLVAQGV AEQLSNRVAF RRAMRKAIQS AMRQPNVKGI RVQCSGRLGG A EMSRSEFY REGRVPLHTL RADIDYGLYE AKTTFGRIGV KVWIYKGDIV GGKRELAAAA PASDRPRRER PSGTRPRRSG SA GTTATST EAGRAATSDA PAAGTAAAAE APAESTES

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #38: Small ribosomal subunit protein uS4

• Macromolecule: Name: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 23.415787 KDa

Sequence

String:

MARYTGPATR KSRRLGVDLV GGDQSFEKRP YPPGQHGRAR IKESEYRQQL QEKQKARFSY GVMEKQFRRY YEEANRQPGK TGDNLLRIL ESRLDNVVYR AGLARTRRMA RQLVSHGHFL VNGVKVDIPS YRVSQYDIID VKEKSLNTLP FQIARETAGE R PIPSWLQV VGERQRILVH QLPERAQIDV PLTEQLIVEL YSK

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #39: Small ribosomal subunit protein uS5

• Macromolecule: Name: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 21.94609 KDa

Sequence

String:

MAEQAGAGSA QDNRGGRGRR DDRGGRGRDG GDKSNYIERV VSINRVSKVV KGGRRFSFTA LVIVGDGKGM VGVGYGKAKE VPAAIAKGV EEARKNFFRV PLIGSTITHP VQGEAAAGVV MLRPASPGTG VIAGGAARAV LECAGVHDIL AKSLGSDNAI N VVHATVAA LKLLQRPEEV AARRGLPIED VAPAGMLKAR RESEALAAAA AREGSA

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #40: Small ribosomal subunit protein bS6

• Macromolecule: Name: Small ribosomal subunit protein bS6 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 10.991637 KDa

Sequence

String:

MRPYEIMVIL DPTLDERTVA PSLETFLNVI RKDGGTVDKV DIWGRRRLAY EIAKHAEGIY AVIDVKAEPA TVSELDRQLN LNESVLRTK VLRTDKH

UniProtKB: Small ribosomal subunit protein bS6

Macromolecule #41: Small ribosomal subunit protein uS7

• Macromolecule: Name: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 17.660375 KDa

Sequence

String:

MPRKGPAPKR PLVNDPVYGS QLVTQLVNKV LLEGKKSLAE RIVYGALEQA REKTGTDPVV TLKRALDNVK PALEVRSRRV GGATYQVPV EVRPDRSTTL ALRWLVNFSR QRREKTMVER LANEILDASN GLGASVKRRE DTHKMAEANR AFAHYRW

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #42: Small ribosomal subunit protein uS8

• Macromolecule: Name: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 14.492638 KDa

Sequence

String:

MTMTDPIADF LTRLRNANSA YHDEVTLPHS KLKANIAEIL KREGYISDYR TEDARVGKSL VVQLKYGPSR ERSIAGLRRV SKPGLRVYA KSTNLPRVLG GLGVAIISTS SGLLTDRQAA RQGVGGEVLA YVW

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #43: Small ribosomal subunit protein uS9

• Macromolecule: Name: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 16.794365 KDa

Sequence

String:

MTDVTETEVV TESAEPREPV IIDRPIQTVG RRKEAVVRVR LVPGTGQFNL DGRTLENYFP NKVHQQLIKA PLVTVDRVDQ FDIYAHLDG GGPSGQAGAL RLAIARALIL VQPEDRPALK KAGFLTRDPR AIERKKYGLK KARKAPQYSK R

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #44: Small ribosomal subunit protein uS10

• Macromolecule: Name: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 11.454313 KDa

Sequence

String:

MAGQKIRIRL KAYDHEAIDA SARKIVETVT RTGASVVGPV PLPTEKNVYC VIRSPHKYKD SREHFEMRTH KRLIDILDPT PKTVDALMR IDLPASVDVN IQ

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #45: Small ribosomal subunit protein uS11

• Macromolecule: Name: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 14.671762 KDa

Sequence

String:

MAQAKKGGTA AKKGQKTRRR EKKNVPHGAA HIKSTFNNTI VSITDPQGNV IAWASSGHVG FKGSRKSTPF AAQLAAENAA RKAQEHGVK KVDVFVKGPG SGRETAIRSL QAAGLEVGTI SDVTPQPHNG CRPPKRRRV

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #46: Small ribosomal subunit protein uS12

• Macromolecule: Name: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 13.896366 KDa

Sequence

String:

MPTIQQLVRK GRRDKIAKVK TAALKGSPQR RGVCTRVYTT TPKKPNSALR KVARVKLTSQ VEVTAYIPGE GHNLQEHSMV LVRGGRVKD LPGVRYKIIR GSLDTQGVKN RKQARSRYGA KKEKS

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #47: Small ribosomal subunit protein uS13

• Macromolecule: Name: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 14.249619 KDa

Sequence

String:

MARLVGVDLP RDKRMEIALT YIYGIGRTRS NEILAATGID KNMRTKDLTD DQVTVLRDYI EGNLKVEGDL RREVQADIRR KIEIGCYQG LRHRRGLPVR GQRTKTNART RKGPKRTIAG KKKAR

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #48: Small ribosomal subunit protein uS14B

• Macromolecule: Name: Small ribosomal subunit protein uS14B / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 6.976409 KDa

Sequence

String:

MAKKALVHKA NKKPKFAVRA YTRCNKCGRP HSVYRKFGLC RICLREMAHA GELPGVQKSS W

UniProtKB: Small ribosomal subunit protein uS14B

Macromolecule #49: Small ribosomal subunit protein uS15

• Macromolecule: Name: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 10.368097 KDa

Sequence

String:

MALTAEQKKE ILGQYGLHDT DTGSPEAQVA LLTKRIQDLT EHLKVHKHDH HSRRGLLLLV GRRRRLLKYV AQVDVARYRS LIERLGLRR

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #50: Small ribosomal subunit protein bS16

• Macromolecule: Name: Small ribosomal subunit protein bS16 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 16.795207 KDa

Sequence

String:

MAVKIKLTRL GKIRNPQYRI IVADARTRRD GRAIEVIGRY HPKEEPSLIQ IDSERAQYWL GVGAQPTEPV LALLKITGDW QKFKGLPGA EGTLKVKEPK PSKLDLFNAA LAEAESGTTA AATTPKKKKA PKKDEAAEAP AEAAEAPAEA ADAASES

UniProtKB: Small ribosomal subunit protein bS16

Macromolecule #51: Small ribosomal subunit protein uS17

• Macromolecule: Name: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 11.127002 KDa

Sequence

String:

MADQKGPKYT PAAEKPRGRR KTAIGYVVSD KMQKTIVVEL EDRKSHPLYG KIIRTTKKVK AHDENGEAGI GDRVSLMETR PLSATKRWR LVEILEKAK

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #52: Small ribosomal subunit protein bS18B

• Macromolecule: Name: Small ribosomal subunit protein bS18B / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 9.524188 KDa

Sequence

String:

MAKSNKRRPA PEKPVKTRKC VFCSKKGQTI DYKDTALLRT YISERGKIRA RRVTGNCVQH QRDIAVAVKN AREVALLPFG SSTR

UniProtKB: Small ribosomal subunit protein bS18B

Macromolecule #53: Small ribosomal subunit protein uS19

• Macromolecule: Name: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 10.800602 KDa

Sequence

String:

MPRSLKKGPF VDDHLLKKVD VQNEKNTKQV IKTWSRRSTI IPDFIGHTFA VHDGRKHVPV FVTEAMVGHK LGEFAPTRTF KGHIKDDRK SKRR

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #54: Small ribosomal subunit protein bS20

• Macromolecule: Name: Small ribosomal subunit protein bS20 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 9.556104 KDa

Sequence

String:

MANIKSQIKR IRTNERRRLR NQSVKSSLRT AIRGFREAVD AGDKDKASEL LHATSRKLDK AASKGVIHPN QAANKKSALA LALNKL

UniProtKB: Small ribosomal subunit protein bS20

Macromolecule #55: Conserved domain protein

• Macromolecule: Name: Conserved domain protein / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 4.1643 KDa

Sequence

String:

MGSVIKKRRK RMSKKKHRKL LRRTRVQRRK LGK

UniProtKB: Conserved domain protein

Macromolecule #9: 23S rRNA

• Macromolecule: Name: 23S rRNA / type: rna / ID: 9 / Number of copies: 1
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 1.012154938 MDa

Sequence

String:

UAAGUGUUUA AGGGCGCAUG GUGGAUGCCU UGGCACUGGG AGCCGAUGAA GGACGUAGGA GGCUGCGAUA AGCCUCGGGG AGCUGUCAA CCGAGCGUUG AUCCGAGGAU GUCCGAAUGG GGAAACCCGG CACGAGUGAU GUCGUGUCAC CAGGCGCUGA A UAUAUAGG CGUCUGGGGG GAACGCGGGG AAGUGAAACA UCUCAGUACC CGUAGGAAGA GAAAACAAAA UGUGAUUCCG UG AGUAGUG GCGAGCGAAA GCGGAGGAUG GCUAAACCGU AUGCAUGUGA UACCGGGUAG GGGUUGUGUG UGCGGGGUUG UGG GACCUA UCUUUCCGGC UCUACCUGGC UGGAGGGCAG UGAGAAAAUG UUGUGGUUAG CGGAAAUGGC UUGGGAUGGC CUGC CGUAG ACGGUGAGAG CCCGGUACGU GAAAACCCGA CGUCUGUCUU GAUGGUGUUC CCGAGUAGCA GCGGGCCCGU GGAAU CUGC UGUGAAUCUG CCGGGACCAC CCGGUAAGCC UGAAUACUUC CCAGUGACCG AUAGCGGAUU AGUACCGUGA GGGAAU GGU GAAAAGUACC CCGGGAGGGG AGUGAAAGAG UACCUGAAAC CGUGCGCUUA CAAUCCGUCA GAGCCCUCGA CGUGUCG UG GGGUGAUGGC GUGCCUUUUG AAGAAUGAGC CUGCGAGUCA GGGACAUGUC GCGAGGUUAA CCCGGGUGGG GUAGCCGC A GCGAAAGCGA GUCUGAAUAG GGCGUAUCCA CACAAGAGUG UGUGGUGUAG UGGUGUGUUC UGGACCCGAA GCGGAGUGA UCUACCCAUG GCCAGGGUGA AGCGCGGGUA AGACCGCGUG GAGGCCCGAA CCCACUUAGG UUGAAGACUG AGGGGAUGAG CUGUGGGUA GGGGUGAAAG GCCAAUCAAA CUCCGUGAUA GCUGGUUCUC CCCGAAAUGC AUUUAGGUGC AGCGUCGCAU G UUUCUUGC CGGAGGUAGA GCUACUGGAU GGCCGAUGGG CCCCACAGGG UUACUGACGU CAGCCAAACU CCGAAUGCCG GU AAGUCCA AGAGUGCGGC AGUGAGACGG CGGGGGAUAA GCUCCGUGCG UCGAGAGGGA AACAGCCCAG AUCGCCGGCU AAG GCCCCU AAGCGUGUGC UAAGUGGAAA AGGAUGUGCA GUCGCGAAGA CAACCAGGAG GUUGGCUUAG AAGCAGCCAC CCUU GAAAG AGUGCGUAAU AGCUCACUGG UCAAGUGAUU GUGCGCCGAU AAUGUAGCGG GGCUCAAGCA CACCGCCGAA GCCGC GGCA GCCAACGUGU UGGCUGGGUA GGGGAGCGUC CUGCAUCCGG UGAAGCCGCC GAGUGAUCGA GUGGUGGAGG GUGUGG GAG UGAGAAUGCA GGCAUGAGUA GCGAUUAGGC AAGUGAGAAC CUUGCCCGCC GAAAGACCAA GGGUUCCUGG GCCAGGC CA GUCCGCCCAG GGUGAGUCGG GACCUAAGGC GAGGCCGACA GGCGUAGUCG AUGGACAACG GGUUGAUAUU CCCGUACC C GUGUAUGUGC GUCCAUGAUG AAUCAGCGGU ACUAACCAUC CAAAACCACC GUGACCGCAC CUUUCGGGGU GUGGCGUUG GUGGGGCUGC AUGGGACCUU CGUUGGUAGU AGUCAAGCGA UGGGGUGACG CAGGAAGGUA GCCGUACCGG UCAGUGGUAA UACCGGGGU AAGCCUGUAG GGAGUCAGAU AGGUAAAUCC GUCUGGCAUA UAUCCUGAGA GGUGAUGCAU AGCCGAGUGA G GCGAAUUC GGUGAUCCUA UGCUGCCGAG AAAAGCCUCU AGCGAGGACA UACACGGCCC GUACCCCAAA CCAACACAGG UG GUCAGGU AGAGAAUACU AAGGCGUACG AGUGAACUAU GGUUAAGGAA CUCGGCAAAA UGCCCCCGUA ACUUCGGGAG AAG GGGGAC CCACAUGGCG UGUAAGCCUU UACGGCCCAA GCGUGAGUGG GUGGCACAAA CCAGUGAGAA GCGACUGUUU ACUA AAAAC ACAGGUCCGU GCGAAGUCGC AAGACGAUGU AUACGGACUG ACGCCUGCCC GGUGCUGGAA GGUUAAGAGG ACCCG UUAA CUCCCUUUGG GGGUGAAGCG GAGAAUUUAA GCCCCAGUAA ACGGCGGUGG UAACUAUAA(OMC) CAUCCUAAGG UA GCGAAAU UCCUUGUCGG GUAAGUUCCG ACCUGCACGA AUGGCGUAAC GACUUCUCAA CUGUCUCAAC CAUAGACUCG GCG AAAUUG CACUACGAGU AAAGAUGCUC GUUACGCGCG GCAGGACGAA AAGACCCCGG GACCUUCACU ACAACUUGGU AUUG GUGCU CGAUACGGUU UGUGUAGGAU AGGUGGGAGA CUGUGAAGCU CACACGCCAG UGUGGGUGGA GUCGUUGUUG AAAUA CCAC UCUGAUCGUA UUGGGCCUCU AACCUCGGAC CGUAUAUCCG GUUCAGGGAC AGUGCCUGGU GGGUAGUUUA ACUGGG GCG GUUGCCUCCU AAAAUGUAAC GGAGGCGCCC AAAGGUUCCC UCAACCUGGA CGGCAAUCAG GUGUUGAGUG UAAGUGC AC AAGGGAGCUU GACUGCGAGA CGGACAUGUC GAGCAGGGAC GAAAGUCGGG ACUAGUGAUC CGGCACCUCU GAGUGGAA G GGGUGUCGCU CAACGGAUAA AAGGUACCCC GGGGAUAACA GGCUGAUCUU CCCCAAGAGU CCAUAUCGAC GGGAUGGUU UGGCACCUCG AUGUCGGCUC GUCGCAUCCU GGGGCUGGAG CAGGUCCCAA GGGUUGGGCU GUUCGCCCAU UAAAGCGGCA CGCGAGCUG GGUUUAGAAC GUCGUGAGAC AGUUCGGUCU CUAUCCGCCG CGCGCGUCAG AAGCUUGAGG AAACCUGUCC C UAGUACGA GAGGACCGGG ACGGACGAAC CUCUGGUAUA CCAGUUGUCC CACCAGGGGC ACGGCUGGAU AGCCACGUUC GG ACAGGAU AACCGCUGAA AGCAUCUAAG CGGGAAACCU CUUCCAAGAC CAGGCUUCUC ACCCUCUAGG AGGGAUAAGG CCC CCCGCA GACCACGGGA UUGAUAGACC AGACCUGGAA GCCUAGUAAU AGGUGCAGGG AACUGGCACU AACCGGCCGA AAAC UUAC

GENBANK: GENBANK: CP080274.1

Macromolecule #10: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 10 / Number of copies: 1
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 38.038777 KDa

Sequence

String:

GUUACGGCGG UCCAUAGCGG CAGGGAAACG CCCGGUCCCA UCCCGAACCC GGAAGCUAAG CCUGCCAGCG CCGAUGAUAC UACCCUUCC GGGUGGAAAA GUAGGACACC GCCGAACAC

GENBANK: GENBANK: CP082846.1

Macromolecule #35: 16S rRNA

• Macromolecule: Name: 16S rRNA / type: rna / ID: 35 / Number of copies: 1
• Source (natural): Organism: Mycolicibacterium smegmatis (bacteria) / Strain: LR222
• Molecular weight: Theoretical: 495.387688 KDa

Sequence

String:

UUUUUGUUUG GAGAGUUUGA UCCUGGCUCA GGACGAACGC UGGCGGCGUG CUUAACACAU GCAAGUCGAA CGGAAAGGCC CUUUCGGGG GUACUCGAGU GGCGAACGGG UGAGUAACAC GUGGGUGAUC UGCCCUGCAC UUUGGGAUAA GCCUGGGAAA C UGGGUCUA AUACCGAAUA CACCCUGCUG GUCGCAUGGC CUGGUAGGGG AAAGCUUUUG CGGUGUGGGA UGGGCCCGCG GC CUAUCAG CUUGUUGGUG GGGUGAUGGC CUACCAAGGC GACGACGGGU AGCCGGCCUG AGAGGGUGAC CGGCCACACU GGG ACUGAG AUACGGCCCA GACUCCUACG GGAGGCAGCA GUGGGGAAUA UUGCACAAUG GGCGCAAGCC UGAUGCAGCG ACGC CGCGU GAGGGAUGAC GGCCUUCGGG UUGUAAACCU CUUUCAGCAC AGACGAAGCG CAAGUGACGG UAUGUGCAGA AGAAG GACC GGCCAACUAC GUGCCAGCAG CCGCGGUAAU ACGUAGGGUC CGAGCGUUGU CCGGAAUUAC UGGGCGUAAA GAGCUC GUA GGUGGUUUGU CGCGUUGUUC GUGAAAACUC ACAGCUUAAC UGUGGGCGUG CGGGCGAUAC GGGCAGACUA GAGUACU GC AGGGGAGACU GGAAUUCCUG GUGUAGCGGU GGAAUGCGCA GAUAUCAGGA GGAACACCGG UGGCGAAGGC GGGUCUCU G GGCAGUAACU GACGCUGAGG AGCGAAAGCG UGGGGAGCGA ACAGGAUUAG AUACCCUGGU AGUCCACGCC GUAAACGGU GGGUACUAGG UGUGGGUUUC CUUCCUUGGG AUCCGUGCCG UAGCUAACGC AUUAAGUACC CCGCCUGGGG AGUACGGCCG CAAGGCUAA AACUCAAAGG AAUUGACGGG GGCCCGCACA AGCGGCGGAG CAUGUGGAUU AAUUCGAUGC AACGCGAAGA A CCUUACCU GGGUUUGACA UGCACAGGAC GCCGGCAGAG AUGUCGGUUC CCUUGUGGCC UGUGUGCAGG UGGUGCAUGG CU GUCGUCA GCUCGUGUCG UGAGAUGUUG GGUUAAGUCC CGCAACGAGC GCAACCCUUG UCUCAUGUUG CCAGCACGUU AUG GUGGGG ACUCGUGAGA GACUGCCGGG GUCAACUCGG AGGAAGGUGG GGAUGACGUC AAGUCAUCAU GCCCCUUAUG UCCA GGGCU UCACACAUGC UACAAUGGCC GGUACAAAGG GCUGCGAUGC CGUGAGGUGG AGCGAAUCCU UUCAAAGCCG GUCUC AGUU CGGAUCGGGG UCUGCAACUC GACCCCGUGA AGUCGGAGUC GCUAGUAAUC GCAGAUCAGC AACGCUGCGG UGAAUA CGU UCCCGGGCCU UGUACACACC GCCCGUCA(OMC)G UCAUGAAAGU CGGUAACACC CGAAGCCGGU GGCCUAACCC UUG UGGAGG GAGCCGUCGA AGGUGGGAUC GGCGAUUGGG ACGAAGUCGU AACAAGGUAG CCGUACCGGA AGGUGCGGCU GGAU CACCU CCUUUCU

GENBANK: GENBANK: CP082846.1

Macromolecule #56: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 56 / Number of copies: 139 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Grid: Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec. / Pretreatment - Atmosphere: OTHER
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Number real images: 5665 / Average exposure time: 2.0 sec. / Average electron dose: 52.51 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.6 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Software - Name: CTFFIND (ver. 4.1) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: OTHER; Details: RELION-3.1 3D initial reconstruction (stochastic gradient descent method)
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.17 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 443500
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final 3D classification: Software - Name: RELION (ver. 3.1)

Atomic model buiding 1

Initial model

PDB ID:

5zeb

Chain - Source name: PDB / Chain - Initial model type: experimental model

• Refinement: Protocol: FLEXIBLE FIT

Output model

PDB-9e0p:
M. smegmatis methylated 70S ribosome structure