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基本情報
登録情報 | ![]() | |||||||||||||||
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タイトル | Resting state 1 of the GluA2-gamma2 complex | |||||||||||||||
![]() | Resting state 1 LBD-TMD locally sharpened map | |||||||||||||||
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![]() | ligand-gated ion channel / ionotropic glutamate receptor / ampa receptor / ion channel / TRANSPORT PROTEIN | |||||||||||||||
機能・相同性 | ![]() Presynaptic depolarization and calcium channel opening / LGI-ADAM interactions / Trafficking of AMPA receptors / eye blink reflex / positive regulation of protein localization to basolateral plasma membrane / cerebellar mossy fiber / postsynaptic neurotransmitter receptor diffusion trapping / regulation of AMPA receptor activity / membrane hyperpolarization / nervous system process ...Presynaptic depolarization and calcium channel opening / LGI-ADAM interactions / Trafficking of AMPA receptors / eye blink reflex / positive regulation of protein localization to basolateral plasma membrane / cerebellar mossy fiber / postsynaptic neurotransmitter receptor diffusion trapping / regulation of AMPA receptor activity / membrane hyperpolarization / nervous system process / protein targeting to membrane / voltage-gated calcium channel complex / spine synapse / neurotransmitter receptor localization to postsynaptic specialization membrane / dendritic spine neck / neuromuscular junction development / dendritic spine head / Activation of AMPA receptors / perisynaptic space / ligand-gated monoatomic cation channel activity / AMPA glutamate receptor activity / transmission of nerve impulse / immunoglobulin binding / Trafficking of GluR2-containing AMPA receptors / response to lithium ion / kainate selective glutamate receptor activity / AMPA glutamate receptor complex / cellular response to glycine / extracellularly glutamate-gated ion channel activity / ionotropic glutamate receptor complex / membrane depolarization / asymmetric synapse / regulation of receptor recycling / Unblocking of NMDA receptors, glutamate binding and activation / glutamate receptor binding / positive regulation of synaptic transmission / regulation of postsynaptic membrane neurotransmitter receptor levels / voltage-gated calcium channel activity / response to fungicide / regulation of synaptic transmission, glutamatergic / glutamate-gated receptor activity / cytoskeletal protein binding / ionotropic glutamate receptor binding / extracellular ligand-gated monoatomic ion channel activity / presynaptic active zone membrane / somatodendritic compartment / cellular response to brain-derived neurotrophic factor stimulus / glutamate-gated calcium ion channel activity / dendrite cytoplasm / dendrite membrane / ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / ionotropic glutamate receptor signaling pathway / SNARE binding / hippocampal mossy fiber to CA3 synapse / dendritic shaft / regulation of membrane potential / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / synaptic transmission, glutamatergic / PDZ domain binding / protein tetramerization / establishment of protein localization / synaptic membrane / modulation of chemical synaptic transmission / postsynaptic density membrane / terminal bouton / cerebral cortex development / Schaffer collateral - CA1 synapse / receptor internalization / response to calcium ion / synaptic vesicle membrane / synaptic vesicle / presynapse / signaling receptor activity / amyloid-beta binding / presynaptic membrane / growth cone / scaffold protein binding / chemical synaptic transmission / perikaryon / dendritic spine / postsynaptic membrane / neuron projection / postsynaptic density / axon / neuronal cell body / synapse / dendrite / protein-containing complex binding / protein kinase binding / glutamatergic synapse / cell surface / endoplasmic reticulum / protein-containing complex / identical protein binding / membrane / plasma membrane 類似検索 - 分子機能 | |||||||||||||||
生物種 | ![]() ![]() ![]() ![]() | |||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.18 Å | |||||||||||||||
![]() | Kumar Mondal A / Carrillo E / Jayaraman V / Twomey EC | |||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Glutamate gating of AMPA-subtype iGluRs at physiological temperatures. 著者: Anish Kumar Mondal / Elisa Carrillo / Vasanthi Jayaraman / Edward C Twomey / ![]() 要旨: Ionotropic glutamate receptors (iGluRs) are tetrameric ligand-gated ion channels that mediate most excitatory neurotransmission. iGluRs are gated by glutamate, where on glutamate binding, they open ...Ionotropic glutamate receptors (iGluRs) are tetrameric ligand-gated ion channels that mediate most excitatory neurotransmission. iGluRs are gated by glutamate, where on glutamate binding, they open their ion channels to enable cation influx into postsynaptic neurons, initiating signal transduction. The structural mechanics of how glutamate gating occurs in full-length iGluRs is not well understood. Here, using the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid subtype iGluR (AMPAR), we identify the glutamate-gating mechanism. AMPAR activation by glutamate is augmented at physiological temperatures. By preparing AMPARs for cryogenic-electron microscopy at these temperatures, we captured the glutamate-gating mechanism. Activation by glutamate initiates ion channel opening that involves all ion channel helices hinging away from the pore axis in a motif that is conserved across all iGluRs. Desensitization occurs when the local dimer pairs decouple and enables closure of the ion channel below through restoring the channel hinges and refolding the channel gate. Our findings define how glutamate gates iGluRs, provide foundations for therapeutic design and demonstrate how physiological temperatures can alter iGluR function. | |||||||||||||||
履歴 |
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構造の表示
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 5.9 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 18.6 KB 18.6 KB | 表示 表示 | ![]() |
画像 | ![]() | 41.8 KB | ||
Filedesc metadata | ![]() | 6.2 KB | ||
その他 | ![]() ![]() | 300.7 MB 300.7 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 1004.8 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1004.3 KB | 表示 | |
XML形式データ | ![]() | 17 KB | 表示 | |
CIF形式データ | ![]() | 20.3 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 9dhpMC ![]() 9dhqC ![]() 9dhrC ![]() 9dhsC ![]() 9dhtC ![]() 9mrkC ![]() 9mrlC ![]() 9mrmC ![]() 9mrnC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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注釈 | Resting state 1 LBD-TMD locally sharpened map | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.97 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: Resting state 1 LBD-TMD half map
ファイル | emd_46872_half_map_1.map | ||||||||||||
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注釈 | Resting state 1 LBD-TMD half map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Resting state 1 LBD-TMD half map
ファイル | emd_46872_half_map_2.map | ||||||||||||
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注釈 | Resting state 1 LBD-TMD half map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
-全体 : GluA2-TARPgamma2 Complex
全体 | 名称: GluA2-TARPgamma2 Complex |
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要素 |
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-超分子 #1: GluA2-TARPgamma2 Complex
超分子 | 名称: GluA2-TARPgamma2 Complex / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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由来(天然) | 生物種: ![]() ![]() |
-分子 #1: Isoform Flip of Glutamate receptor 2
分子 | 名称: Isoform Flip of Glutamate receptor 2 / タイプ: protein_or_peptide / ID: 1 / コピー数: 4 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 47.938141 KDa |
組換発現 | 生物種: ![]() |
配列 | 文字列: EQKTVVVTTI LESPYVMMKK NHEMLEGNER YEGYCVDLAA EIAKHCGFKY KLTIVGDGKY GARDADTKIW NGMVGELVYG KADIAIAPL TITLVREEVI DFSKPFMSLG ISIMIKKPQK SKPGVFSFLD PLAYEIWMCI VFAYIGVSVV LFLVSRFSPY E WHTEEFED ...文字列: EQKTVVVTTI LESPYVMMKK NHEMLEGNER YEGYCVDLAA EIAKHCGFKY KLTIVGDGKY GARDADTKIW NGMVGELVYG KADIAIAPL TITLVREEVI DFSKPFMSLG ISIMIKKPQK SKPGVFSFLD PLAYEIWMCI VFAYIGVSVV LFLVSRFSPY E WHTEEFED GRETQSSEST NEFGIFNSLW FSLGAFMQQG CDISPRSLSG RIVGGVWWFF TLIIISSYTA NLAAFLTVER MV SPIESAE DLSKQTEIAY GTLDSGSTKE FFRRSKIAVF DKMWTYMRSA EPSVFVRTTA EGVARVRKSK GKYAYLLEST MNE YIEQRK PCDTMKVGGN LDSKGYGIAT PKGSSLGTPV NLAVLKLSEQ GVLDKLKNKW WYDKGECGAK DSGSKEKTSA LSLS NVAGV FYILVGGLGL AMLVALIEFC YKSRA UniProtKB: Glutamate receptor 2 |
-分子 #2: Voltage-dependent calcium channel gamma-2 subunit
分子 | 名称: Voltage-dependent calcium channel gamma-2 subunit / タイプ: protein_or_peptide / ID: 2 / コピー数: 4 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 22.633041 KDa |
組換発現 | 生物種: ![]() |
配列 | 文字列: RGVQMLLTTV GAFAAFSLMT IAVGTDYWLY SRGVCKTKSV SENETSKKNE EVMTHSGLWR TCCLEGNFKG LCKQIDHFPE DADYEADTA EYFLRAVRAS SIFPILSVIL LFMGGLCIAA SEFYKTRHNI ILSAGIFFVS AGLSNIIGII VYISANAGDP S KSDSKKNS ...文字列: RGVQMLLTTV GAFAAFSLMT IAVGTDYWLY SRGVCKTKSV SENETSKKNE EVMTHSGLWR TCCLEGNFKG LCKQIDHFPE DADYEADTA EYFLRAVRAS SIFPILSVIL LFMGGLCIAA SEFYKTRHNI ILSAGIFFVS AGLSNIIGII VYISANAGDP S KSDSKKNS YSYGWSFYFG ALSFIIAEMV GVLAVHMFID RHKQLTG UniProtKB: Voltage-dependent calcium channel gamma-2 subunit |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 4 mg/mL |
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緩衝液 | pH: 8 |
凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | TFS KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON IV (4k x 4k) 平均電子線量: 40.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 1.0 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |