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- EMDB-46504: Cryo-EM structure of mycocerosic acid synthase with double KS-ACP... -
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Open data
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Basic information
Entry | ![]() | ||||||||||||
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Title | Cryo-EM structure of mycocerosic acid synthase with double KS-ACP crosslinking using C16 alpha-bromoamide. Complex A | ||||||||||||
![]() | main map | ||||||||||||
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![]() | FAS / polyketide / crosslinked / ketosynthase / BIOSYNTHETIC PROTEIN | ||||||||||||
Function / homology | ![]() mycocerosate synthase / DIM/DIP cell wall layer assembly / fatty acid synthase activity / phosphopantetheine binding / 3-oxoacyl-[acyl-carrier-protein] synthase activity / fatty acid biosynthetic process / oxidoreductase activity / plasma membrane / cytoplasm Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.87 Å | ||||||||||||
![]() | Heberlig GW / Jiang Z / Burkart MD | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Visualizing Acyl Carrier Protein Interactions within a Crosslinked Type I Polyketide Synthase Authors: Jiang Z / Heberlig GW / Chen JA / Huynh J / La Clair JJ / Burkart MD | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 197.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 21.3 KB 21.3 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 12.6 KB | Display | ![]() |
Images | ![]() | 163.1 KB | ||
Filedesc metadata | ![]() | 7.8 KB | ||
Others | ![]() ![]() | 194 MB 194 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 21.3 KB | Display | |
Data in CIF | ![]() | 27.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 9d2yMC ![]() 9d2zC ![]() 9d30C M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||
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Annotation | main map | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.889 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: half A
File | emd_46504_half_map_1.map | ||||||||||||
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Annotation | half A | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half B
File | emd_46504_half_map_2.map | ||||||||||||
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Annotation | half B | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Homodimeric MAS doubly crosslinked between ACP and KS domains
Entire | Name: Homodimeric MAS doubly crosslinked between ACP and KS domains |
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Components |
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-Supramolecule #1: Homodimeric MAS doubly crosslinked between ACP and KS domains
Supramolecule | Name: Homodimeric MAS doubly crosslinked between ACP and KS domains type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Multifunctional mycocerosic acid synthase membrane-associated MAS
Macromolecule | Name: Multifunctional mycocerosic acid synthase membrane-associated MAS type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO / EC number: mycocerosate synthase |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 226.131672 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MESRVTPVAV IGMGCRLPGG INSPDKLWES LLRGDDLVTE IPPDRWDADD YYDPEPGVPG RSVSRWGGFL DDVAGFDAEF FGISEREAT SIDPQQRLLL ETSWEAIEHA GLDPASLAGS STAVFTGLTH EDYLVLTTTA GGLASPYVVT GLNNSVASGR I AHTLGLHG ...String: MESRVTPVAV IGMGCRLPGG INSPDKLWES LLRGDDLVTE IPPDRWDADD YYDPEPGVPG RSVSRWGGFL DDVAGFDAEF FGISEREAT SIDPQQRLLL ETSWEAIEHA GLDPASLAGS STAVFTGLTH EDYLVLTTTA GGLASPYVVT GLNNSVASGR I AHTLGLHG PAMTFDTACS SGLMAVHLAC RSLHDGEADL ALAGGCAVLL EPHASVAASA QGMLSSTGRC HSFDADADGF VR SEGCAMV LLKRLPDALR DGNRIFAVVR GTATNQDGRT ETLTMPSEDA QVAVYRAALA AAGVQPETVG VVEAHGTGTP IGD PIEYRS LARVYGAGTP CALGSAKSNM GHSTASAGTV GLIKAILSLR HGVVPPLLHF NRLPDELSDV ETGLFVPQAV TPWP NGNDH TPKRVAVSSF GMSGTNVHAI VEEAPAEASA PESSPGDAEV GPRLFMLSST SSDALRQTAR QLATWVEEHQ DCVAA SDLA YTLARGRAHR PVRTAVVAAN LPELVEGLRE VADGDALYDA AVGHGDRGPV WVFSGQGSQW AAMGTQLLAS EPVFAA TIA KLEPVIAAES GFSVTEAITA QQTVTGIDKV QPAVFAVQVA LAATMEQTYG VRPGAVVGHS MGESAAAVVA GALSLED AA RVICRRSKLM TRIAGAGAMG SVELPAKQVN SELMARGIDD VVVSVVASPQ STVIGGTSDT VRDLIARWEQ RDVMAREV A VDVASHSPQV DPILDDLAAA LADIAPMTPK VPYYSATLFD PREQPVCDGA YWVDNLRNTV QFAAAVQAAM EDGYRVFAE LSPHPLLTHA VEQTGRSLDM SVAALAGMRR EQPLPHGLRG LLTELHRAGA ALDYSALYPA GRLVDAPLPA WTHARLFIDD DGQEQRAQG ACTITVHPLL GSHVRLTEEP ERHVWQGDVG TSVLSWLSDH QVHNVAALPG AAYCEMALAA AAEVFGEAAE V RDITFEQM LLLDEQTPID AVASIDAPGV VNFTVETNRD GETTRHATAA LRAAEDDCPP PGYDITALLQ AHPHAVNGTA MR ESFAERG VTLGAAFGGL TTAHTAEAGA ATVLAEVALP ASIRFQQGAY RIHPALLDAC FQSVGAGVQA GTATGGLLLP LGV RSLRAY GPTRNARYCY TRLTKAFNDG TRGGEADLDV LDQHGTVLLA VRGLRMGTGT SERDERDRLV SERLLTLGWQ QRAL PEVGD GEAGSWLLID TSNAVDTPDM LASTLTDALK SHGPQGTECA SLSWSVQDTP PNDQAGLEKL GSQLRGRDGV VIVYG PRVG DPDEHSLLAG REQVRHLVRI TRELAEFEGE LPRLFVVTRQ AQIVKPHDSG ERANLEQAGL RGLLRVISSE HPMLRT TLI DVDEHTDVER VAQQLLSGSE EDETAWRNGD WYVARLTPSP LGHEERRTAV LDPDHDGMRV QVRRPGDLQT LEFVASD RV PPGPGQIEVA VSMSSINFAD VLIAFGRFPI IDDREPQLGM DFVGVVTAVG EGVTGHQVGD RVGGFSEGGC WRTFLTCD A NLAVTLPPGL TDEQAITAAT AHATAWYGLN DLAQIKAGDK VLIHSATGGV GQAAISIARA KGAEIFATAG NPAKRAMLR DMGVEHVYDS RSVEFAEQIR RDTDGYGVDI VLNSLTGAAQ RAGLELLAFG GRFVEIGKAD VYGNTRLGLF PFRRGLTFYY LDLALMSVT QPDRVRELLA TVFKLTADGV LTAPQCTHYP LAEAADAIRA MSNAEHTGKL VLDVPRSGRR SVAVTPEQAP L YRRDGSYI ITGGLGGLGL FFASKLAAAG CGRIVLTARS QPNPKARQTI EGLRAAGADI VVECGNIAEP DTADRLVSAA TA TGLPLRG VLHSAAVVED ATLTNITDEL IDRDWSPKVF GSWNLHRATL GQPLDWFCLF SSGAALLGSP GQGAYAAANS WVD VFAHWR RAQGLPVSAI AWGAWGEVGR ATFLAEGGEI MITPEEGAYA FETLVRHDRA YSGYIPILGA PWLADLVRRS PWGE MFAST GQRSRGPSKF RMELLSLPQD EWAGRLRRLL VEQASVILRR TIDADRSFIE YGLDSLGMLE MRTHVETETG IRLTP KVIA TNNTARALAQ YLADTLAEEQ AAAPAASKLA AALEHHHHHH UniProtKB: Multifunctional mycocerosic acid synthase membrane-associated MAS |
-Macromolecule #2: C16 alpha-bromoamide
Macromolecule | Name: C16 alpha-bromoamide / type: ligand / ID: 2 / Number of copies: 2 / Formula: A1BY8 |
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Molecular weight | Theoretical: 658.603 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1.0 mg/mL | ||||||
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Buffer | pH: 7.4 Component:
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE | ||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | TFS KRIOS |
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Specialist optics | Energy filter - Name: TFS Selectris X / Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Number grids imaged: 1 / Number real images: 3135 / Average electron dose: 55.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 130000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |