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- EMDB-45302: mouse Seipin complex -

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Basic information

Entry
Database: EMDB / ID: EMD-45302
Titlemouse Seipin complex
Map datamouse seipin complex
Sample
  • Complex: Seipin complex
    • Protein or peptide: Seipin
Keywordsseipin / lipid droplets / adipose / MEMBRANE PROTEIN
Function / homologySeipin family / Putative adipose-regulatory protein (Seipin) / lipid droplet formation / lipid droplet / phospholipid binding / lipid metabolic process / endoplasmic reticulum membrane / Seipin
Function and homology information
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.19 Å
AuthorsLi C / Han Y / Wynn RM / Chen Z / Scherer PE
Funding support United States, 5 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)RC2-DK118620 United States
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)R01- DK55758 United States
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)R01-DK099110 United States
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)R01-DK127274 United States
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)R01-DK131537 United States
CitationJournal: To Be Published
Title: Adipogenin Dictates Adipose Tissue Expansion by Facilitating the Assembly of a Dodecameric Seipin Complex
Authors: Li C / Sun X
History
DepositionJun 12, 2024-
Header (metadata) releaseAug 27, 2025-
Map releaseAug 27, 2025-
UpdateAug 27, 2025-
Current statusAug 27, 2025Processing site: RCSB / Status: Released

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Structure visualization

Downloads & links

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Map

FileDownload / File: emd_45302.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationmouse seipin complex
Voxel sizeX=Y=Z: 0.8344 Å
Density
Contour LevelBy AUTHOR: 0.08
Minimum - Maximum-0.0018974592 - 2.0808237
Average (Standard dev.)0.0011424852 (±0.025523048)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 320.4096 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Seipin complex

EntireName: Seipin complex
Components
  • Complex: Seipin complex
    • Protein or peptide: Seipin

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Supramolecule #1: Seipin complex

SupramoleculeName: Seipin complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 700 KDa

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Macromolecule #1: Seipin

MacromoleculeName: Seipin / type: protein_or_peptide / ID: 1 / Number of copies: 11 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 50.993406 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MSGRWSHPQF EKIHQRREAG ARETCRDQIK GSDKDEEPSA ALSHGQGYRP CGRPARNSKP EAGARPPAVP IMVNDPPVPA LLWAQEVGH VLAGRARRLM LQFGVLFCTI LLLLWVSVFL YGSFYYSYMP TVSHLSPVHF HYRTDCDSST ASLCSFPVAN V SLAKSGRD ...String:
MSGRWSHPQF EKIHQRREAG ARETCRDQIK GSDKDEEPSA ALSHGQGYRP CGRPARNSKP EAGARPPAVP IMVNDPPVPA LLWAQEVGH VLAGRARRLM LQFGVLFCTI LLLLWVSVFL YGSFYYSYMP TVSHLSPVHF HYRTDCDSST ASLCSFPVAN V SLAKSGRD RVLMYGQPYR VTLELELPES PVNQDLGMFL VTVSCYTRGG RIISTSSRSV MLHYRSQLLQ VLDTLLFSSL LL FGFAEQK QLLEVELYSD YRENSYVPTT GAIIEIHSKR IQMYGAYLRI HAHFTGLRYL LYNFPMTCAF VGVASNFTFL SVI VLFSYM QWVWGAVWPR HRFSLQVNIR QRDNSHHGAP RRISRHQPGQ ESTQQSDVTE DGESPEDPSG TEGQLSEEEK PEKR PLNGE EEQEPEASDG SWEDAALLTE ANPPTSASAS ALAPETLGSL RQRPTCSSS

UniProtKB: Seipin

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - Material: GRAPHENE OXIDE / Support film - topology: CONTINUOUS
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionApplied symmetry - Point group: C11 (11 fold cyclic) / Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 3.19 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 17212
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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