+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-45293 | ||||||||||||
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Title | Structure of the human BOS complex in GDN | ||||||||||||
Map data | |||||||||||||
Sample |
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Keywords | membrane protein biogenesis / membrane protein complex / MEMBRANE PROTEIN | ||||||||||||
Biological species | Homo sapiens (human) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 8.85 Å | ||||||||||||
Authors | Nguyen VN / Tomaleri GP / Voorhees RM | ||||||||||||
Funding support | United States, 3 items
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Citation | Journal: To Be Published Title: Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins Authors: Page KR / Nguyen VN / Pleiner T / Tomaleri GP / Wang ML / Guna A / Hazu M / Wang T / Chou T / Voorhees RM | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_45293.map.gz | 200.5 MB | EMDB map data format | |
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Header (meta data) | emd-45293-v30.xml emd-45293.xml | 15.9 KB 15.9 KB | Display Display | EMDB header |
Images | emd_45293.png | 21.1 KB | ||
Filedesc metadata | emd-45293.cif.gz | 4.6 KB | ||
Others | emd_45293_half_map_1.map.gz emd_45293_half_map_2.map.gz | 391.8 MB 391.8 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-45293 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-45293 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_45293.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.832 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_45293_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_45293_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Human BOS complex
Entire | Name: Human BOS complex |
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Components |
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-Supramolecule #1: Human BOS complex
Supramolecule | Name: Human BOS complex / type: complex / ID: 1 / Parent: 0 / Details: Human BOS complex of NOMO, TMEM147, and NCLN |
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Source (natural) | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 2.48 mg/mL | ||||||||||||||||||
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Buffer | pH: 7.5 Component:
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Grid | Model: UltrAuFoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. / Pretreatment - Atmosphere: AIR | ||||||||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 279 K / Instrument: FEI VITROBOT MARK IV | ||||||||||||||||||
Details | Sample solubilized and purified in GDN. |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Energy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 7174 / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: DARK FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 105000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |