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- EMDB-44944: TnsABCD-DNA transpososome -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-44944
TitleTnsABCD-DNA transpososome
Map data
Sample
  • Complex: TnsABCD-DNA complex
    • Complex: TnsABCD
      • Protein or peptide: x 3 types
    • Complex: DNA
      • DNA: x 6 types
  • Ligand: x 4 types
KeywordsTn7-like transposon / type I-B2 CAST / DNA binding protein / transpososome / DNA BINDING PROTEIN-DNA complex
Function / homology
Function and homology information


DNA integration / nucleic acid binding / ATP hydrolysis activity
Similarity search - Function
TnsA endonuclease C-terminal / TnsA endonuclease, N-terminal / TnsA endonuclease C terminal / TnsA-like endonuclease N terminal / Transposase-like, Mu, C-terminal / Mu transposase, C-terminal / TniQ / TniQ / : / AAA domain ...TnsA endonuclease C-terminal / TnsA endonuclease, N-terminal / TnsA endonuclease C terminal / TnsA-like endonuclease N terminal / Transposase-like, Mu, C-terminal / Mu transposase, C-terminal / TniQ / TniQ / : / AAA domain / Cro/C1-type HTH domain profile. / Cro/C1-type helix-turn-helix domain / Integrase, catalytic core / Integrase catalytic domain profile. / Ribonuclease H superfamily / Ribonuclease H-like superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
ORC1/DEAH AAA+ ATPase domain-containing protein / Integrase / HTH cro/C1-type domain-containing protein
Similarity search - Component
Biological speciesPeltigera membranacea (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.65 Å
AuthorsChang L / Wang S
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) United States
CitationJournal: Cell / Year: 2024
Title: Structure of TnsABCD transpososome reveals mechanisms of targeted DNA transposition.
Authors: Shukun Wang / Romana Siddique / Mark C Hall / Phoebe A Rice / Leifu Chang /
Abstract: Tn7-like transposons are characterized by their ability to insert specifically into host chromosomes. Recognition of the attachment (att) site by TnsD recruits the TnsABC proteins to form the ...Tn7-like transposons are characterized by their ability to insert specifically into host chromosomes. Recognition of the attachment (att) site by TnsD recruits the TnsABC proteins to form the transpososome and facilitate transposition. Although this pathway is well established, atomic-level structural insights of this process remain largely elusive. Here, we present the cryo-electron microscopy (cryo-EM) structures of the TnsC-TnsD-att DNA complex and the TnsABCD transpososome from the Tn7-like transposon in Peltigera membranacea cyanobiont 210A, a type I-B CRISPR-associated transposon. Our structures reveal a striking bending of the att DNA, featured by the intercalation of an arginine side chain of TnsD into a CC/GG dinucleotide step. The TnsABCD transpososome structure reveals TnsA-TnsB interactions and demonstrates that TnsC not only recruits TnsAB but also directly participates in the transpososome assembly. These findings provide mechanistic insights into targeted DNA insertion by Tn7-like transposons, with implications for improving the precision and efficiency of their genome-editing applications.
History
DepositionMay 20, 2024-
Header (metadata) releaseOct 16, 2024-
Map releaseOct 16, 2024-
UpdateMay 28, 2025-
Current statusMay 28, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_44944.png

Downloads & links

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Map

FileDownload / File: emd_44944.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 480 pix.
= 394.56 Å		0.82 Å/pix.
x 480 pix.
= 394.56 Å		0.82 Å/pix.
x 480 pix.
= 394.56 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.822 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.01
Minimum - Maximum-0.002413669 - 2.2509682
Average (Standard dev.)0.0015596119 (±0.025734948)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 394.56 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_44944_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_44944_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : TnsABCD-DNA complex

EntireName: TnsABCD-DNA complex
Components
  • Complex: TnsABCD-DNA complex
    • Complex: TnsABCD
      • Protein or peptide: TnsD
      • Protein or peptide: Integrase
      • Protein or peptide: TnsC
    • Complex: DNA
      • DNA: LE_polyA
      • DNA: RE_target
      • DNA: RE_polyA
      • DNA: Target_downstream
      • DNA: Target_val
      • DNA: tRNA_LE_LUEGO
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: water

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Supramolecule #1: TnsABCD-DNA complex

SupramoleculeName: TnsABCD-DNA complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#9

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Supramolecule #2: TnsABCD

SupramoleculeName: TnsABCD / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #7-#9
Source (natural)Organism: Peltigera membranacea (fungus)

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Supramolecule #3: DNA

SupramoleculeName: DNA / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1-#6
Source (natural)Organism: Peltigera membranacea (fungus) / Synthetically produced: Yes

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Macromolecule #1: LE_polyA

MacromoleculeName: LE_polyA / type: dna / ID: 1 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Peltigera membranacea (fungus)
Molecular weightTheoretical: 26.308939 KDa
SequenceString: (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DT)(DG)(DT)(DG)(DA) (DC)(DT)(DT)(DT)(DA)(DC)(DC)(DC)(DA) (DT)(DA)(DA)(DC)(DT)(DT)(DT)(DG)(DC)(DC) (DG) (DC)(DA)(DA)(DC)(DG)(DG) ...String:
(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DT)(DG)(DT)(DG)(DA) (DC)(DT)(DT)(DT)(DA)(DC)(DC)(DC)(DA) (DT)(DA)(DA)(DC)(DT)(DT)(DT)(DG)(DC)(DC) (DG) (DC)(DA)(DA)(DC)(DG)(DG)(DC)(DA) (DA)(DG)(DC)(DT)(DT)(DA)(DT)(DG)(DC)(DT) (DT)(DC) (DC)(DA)(DA)(DT)(DA)(DA)(DA) (DG)(DG)(DG)(DT)(DG)(DG)(DC)(DA)(DA)(DG) (DG)(DT)(DT) (DA)(DT)(DG)(DG)(DT)

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Macromolecule #2: RE_target

MacromoleculeName: RE_target / type: dna / ID: 2 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Peltigera membranacea (fungus)
Molecular weightTheoretical: 61.828516 KDa
SequenceString: (DT)(DC)(DA)(DC)(DG)(DG)(DC)(DA)(DA)(DG) (DC)(DT)(DT)(DA)(DT)(DC)(DC)(DT)(DT)(DC) (DC)(DA)(DA)(DT)(DT)(DT)(DG)(DG)(DA) (DC)(DT)(DA)(DG)(DC)(DG)(DG)(DC)(DA)(DA) (DA) (DG)(DT)(DT)(DA)(DT)(DG) ...String:
(DT)(DC)(DA)(DC)(DG)(DG)(DC)(DA)(DA)(DG) (DC)(DT)(DT)(DA)(DT)(DC)(DC)(DT)(DT)(DC) (DC)(DA)(DA)(DT)(DT)(DT)(DG)(DG)(DA) (DC)(DT)(DA)(DG)(DC)(DG)(DG)(DC)(DA)(DA) (DA) (DG)(DT)(DT)(DA)(DT)(DG)(DG)(DT) (DT)(DC)(DC)(DA)(DA)(DA)(DC)(DT)(DG)(DT) (DC)(DA) (DA)(DT)(DT)(DT)(DC)(DA)(DT) (DC)(DC)(DA)(DA)(DA)(DA)(DT)(DT)(DA)(DG) (DC)(DT)(DG) (DA)(DC)(DG)(DC)(DA)(DA) (DG)(DA)(DA)(DT)(DG)(DA)(DG)(DG)(DG)(DT) (DT)(DG)(DT)(DA) (DG)(DT)(DG)(DT)(DT) (DG)(DT)(DT)(DG)(DA)(DC)(DG)(DG)(DC)(DA) (DA)(DA)(DG)(DT)(DT) (DA)(DT)(DG)(DG) (DT)(DT)(DC)(DC)(DA)(DG)(DT)(DA)(DA)(DC) (DG)(DG)(DC)(DA)(DA)(DA) (DG)(DT)(DT) (DA)(DT)(DG)(DG)(DG)(DT)(DA)(DA)(DA)(DG) (DT)(DC)(DA)(DC)(DA)(DC)(DT) (DA)(DC) (DG)(DT)(DC)(DT)(DT)(DT)(DA)(DA)(DT)(DG) (DG)(DG)(DC)(DA)(DG)(DC)(DG)(DC) (DC) (DC)(DA)(DC)(DA)(DT)(DA)(DC)(DG)(DC)(DA) (DG)(DC)(DG)(DA)(DT)(DT)(DT)(DC)(DC)

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Macromolecule #3: RE_polyA

MacromoleculeName: RE_polyA / type: dna / ID: 3 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Peltigera membranacea (fungus)
Molecular weightTheoretical: 53.267184 KDa
SequenceString: (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DT)(DG)(DT)(DG)(DA) (DC)(DT)(DT)(DT)(DA)(DC)(DC)(DC)(DA) (DT)(DA)(DA)(DC)(DT)(DT)(DT)(DG)(DC)(DC) (DG) (DT)(DT)(DA)(DC)(DT)(DG) ...String:
(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DT)(DG)(DT)(DG)(DA) (DC)(DT)(DT)(DT)(DA)(DC)(DC)(DC)(DA) (DT)(DA)(DA)(DC)(DT)(DT)(DT)(DG)(DC)(DC) (DG) (DT)(DT)(DA)(DC)(DT)(DG)(DG)(DA) (DA)(DC)(DC)(DA)(DT)(DA)(DA)(DC)(DT)(DT) (DT)(DG) (DC)(DC)(DG)(DT)(DC)(DA)(DA) (DC)(DA)(DA)(DC)(DA)(DC)(DT)(DA)(DC)(DA) (DA)(DC)(DC) (DC)(DT)(DC)(DA)(DT)(DT) (DC)(DT)(DT)(DG)(DC)(DG)(DT)(DC)(DA)(DG) (DC)(DT)(DA)(DA) (DT)(DT)(DT)(DT)(DG) (DG)(DA)(DT)(DG)(DA)(DA)(DA)(DT)(DT)(DG) (DA)(DC)(DA)(DG)(DT) (DT)(DT)(DG)(DG) (DA)(DA)(DC)(DC)(DA)(DT)(DA)(DA)(DC)(DT) (DT)(DT)(DG)(DC)(DC)(DG) (DC)(DT)(DA) (DG)(DT)(DC)(DC)(DA)(DA)(DA)(DT)(DT)(DG) (DG)(DA)(DA)(DG)(DG)(DA)(DT) (DA)(DA) (DG)(DC)(DT)(DT)(DG)(DC)(DC)(DG)(DT)(DG) (DA)

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Macromolecule #4: Target_downstream

MacromoleculeName: Target_downstream / type: dna / ID: 4 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Peltigera membranacea (fungus)
Molecular weightTheoretical: 11.47137 KDa
SequenceString:
(DG)(DG)(DA)(DA)(DA)(DT)(DC)(DG)(DC)(DT) (DG)(DC)(DG)(DT)(DA)(DT)(DG)(DT)(DG)(DG) (DG)(DC)(DG)(DC)(DT)(DG)(DC)(DC)(DC) (DA)(DT)(DT)(DA)(DA)(DA)(DG)(DA)

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Macromolecule #5: Target_val

MacromoleculeName: Target_val / type: dna / ID: 5 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Peltigera membranacea (fungus)
Molecular weightTheoretical: 21.278615 KDa
SequenceString: (DC)(DC)(DT)(DT)(DA)(DC)(DA)(DA)(DG)(DC) (DA)(DG)(DG)(DA)(DT)(DG)(DT)(DC)(DA)(DT) (DC)(DA)(DG)(DT)(DT)(DC)(DG)(DA)(DG) (DT)(DC)(DT)(DG)(DG)(DT)(DA)(DC)(DT)(DG) (DC) (DC)(DC)(DA)(DG)(DT)(DA) ...String:
(DC)(DC)(DT)(DT)(DA)(DC)(DA)(DA)(DG)(DC) (DA)(DG)(DG)(DA)(DT)(DG)(DT)(DC)(DA)(DT) (DC)(DA)(DG)(DT)(DT)(DC)(DG)(DA)(DG) (DT)(DC)(DT)(DG)(DG)(DT)(DA)(DC)(DT)(DG) (DC) (DC)(DC)(DA)(DG)(DT)(DA)(DG)(DT) (DG)(DA)(DT)(DC)(DT)(DT)(DA)(DT)(DT)(DT) (DC)(DA) (DT)(DT)(DA)(DT)(DG)(DG)(DT) (DG)(DA)

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Macromolecule #6: tRNA_LE_LUEGO

MacromoleculeName: tRNA_LE_LUEGO / type: dna / ID: 6 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Peltigera membranacea (fungus)
Molecular weightTheoretical: 48.725188 KDa
SequenceString: (DA)(DC)(DC)(DA)(DT)(DA)(DA)(DC)(DC)(DT) (DT)(DG)(DC)(DC)(DA)(DC)(DC)(DC)(DT)(DT) (DT)(DA)(DT)(DT)(DG)(DG)(DA)(DA)(DG) (DC)(DA)(DT)(DA)(DA)(DG)(DC)(DT)(DT)(DG) (DC) (DC)(DG)(DT)(DT)(DG)(DC) ...String:
(DA)(DC)(DC)(DA)(DT)(DA)(DA)(DC)(DC)(DT) (DT)(DG)(DC)(DC)(DA)(DC)(DC)(DC)(DT)(DT) (DT)(DA)(DT)(DT)(DG)(DG)(DA)(DA)(DG) (DC)(DA)(DT)(DA)(DA)(DG)(DC)(DT)(DT)(DG) (DC) (DC)(DG)(DT)(DT)(DG)(DC)(DG)(DG) (DC)(DA)(DA)(DA)(DG)(DT)(DT)(DA)(DT)(DG) (DG)(DG) (DT)(DA)(DA)(DA)(DG)(DT)(DC) (DA)(DC)(DA)(DC)(DG)(DT)(DA)(DG)(DT)(DC) (DA)(DC)(DC) (DA)(DT)(DA)(DA)(DT)(DG) (DA)(DA)(DA)(DT)(DA)(DA)(DG)(DA)(DT)(DC) (DA)(DC)(DT)(DA) (DC)(DT)(DG)(DG)(DG) (DC)(DA)(DG)(DT)(DA)(DC)(DC)(DA)(DG)(DA) (DC)(DT)(DC)(DG)(DA) (DA)(DC)(DT)(DG) (DA)(DT)(DG)(DA)(DC)(DA)(DT)(DC)(DC)(DT) (DG)(DC)(DT)(DT)(DG)(DT) (DA)(DA)(DG) (DG)(DC)(DC)(DA)(DG)(DA)(DC)(DC)(DA)(DG) (DG)(DG)(DC)(DA)(DC)

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Macromolecule #7: TnsD

MacromoleculeName: TnsD / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Peltigera membranacea (fungus)
Molecular weightTheoretical: 52.821109 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MLASVLETYE SWNLKKPLIP QRSRLYQPQP VGIGTPYIES LTGYITRIAE LHGVLPGVLM TREIAPLVNK IYFQNGANRG FREIFNRSQ ALNGMGEMAA DLVQVLQKLT LRDDLRFLTM LFWSNILTPR NLFRTRKAWC PICYQERHQN GLVVYEQLLW T INLITICP ...String:
MLASVLETYE SWNLKKPLIP QRSRLYQPQP VGIGTPYIES LTGYITRIAE LHGVLPGVLM TREIAPLVNK IYFQNGANRG FREIFNRSQ ALNGMGEMAA DLVQVLQKLT LRDDLRFLTM LFWSNILTPR NLFRTRKAWC PICYQERHQN GLVVYEQLLW T INLITICP QHQKPLVELC PHCNHESPLL NWRSRPGYCS KCGEWLGANQ CLKTFTDGEG SIKLQLEWQY WTANVVGELI LA SQCFESA PSKENITKSL NIVIDKVAEN NAAAFSRLIG VPKNSLWMWQ STKTLPELNT LLKICYELEI SLVEFLTPKN LIT KSFTKI SQKHLQLSRT PRVSPKSFDQ YQVKDALLAI LAGNEEPPPT MEEVGKRLGH HNRTISRHFP DLCSAISAKC RNYN KACRL KSIEKLCSEV REIVLSLNAQ GVYPTEGRVC ELMPNPGCFR YKQVRAAFND ARREFGL

UniProtKB: HTH cro/C1-type domain-containing protein

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Macromolecule #8: Integrase

MacromoleculeName: Integrase / type: protein_or_peptide / ID: 8 / Number of copies: 8 / Enantiomer: LEVO
Source (natural)Organism: Peltigera membranacea (fungus)
Molecular weightTheoretical: 103.015461 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MKEQFEQWVA RLQISEAGQK IIEKVRSSEP SRRVGGGSKN VSGRYPSRKM GVTIQFESHR VALPIIYQLE HDKDVLEFYD QPPQIKLDY QASNGRRLGV LHTPDLFVIR TNSAGWEECK TEQDLKKLAE KNPNRYFYSE ENNQWHSLPG EVYANQFGLY H RIHSDREI ...String:
MKEQFEQWVA RLQISEAGQK IIEKVRSSEP SRRVGGGSKN VSGRYPSRKM GVTIQFESHR VALPIIYQLE HDKDVLEFYD QPPQIKLDY QASNGRRLGV LHTPDLFVIR TNSAGWEECK TEQDLKKLAE KNPNRYFYSE ENNQWHSLPG EVYANQFGLY H RIHSDREI NWVLQRNLQF LEDYYRSESL VVEEAIAQSL LAIVSSQPGI TLAELLNRST GAKSDDIYTL IIQEQIYIDL NA TPLAEPE RCLIFRDEQT ASAYRLMVEQ PSVSLPAISP VVNIVTGATV SWDGKGLNII HVGETEVILG AENNQLIELK KAI FENLVR QGKITSLQTP EKTAISTESW QRFHQASPED QAEALERYKT IEPYLNGHPP ENETIPARTI RHWKAKYLIA IQKY GCGYI GLLSHRSVKG NRQRKLPEDT LAIMEKFILE DYETLKQKRM WEVHAALVRA CEEAGVIAPS YKAFTKEVQR RTGYE QTKK RQGRRAAYQH ESFYWELAIT TPRHGDRPFE IGHIAHTELD VELVCSDTGR NLGRPWATFL VDAYSRRLLA VYLTFD SPS YRSCLMVLRI CVKRHGRLPQ IVVVDNGAEF HSVYFETLLA TFECTKKQRP PAKARFGSVC ERLFGTSNTQ FVHNLLG NT QITRNVRQVT KSVNPKNLAV WTLGLLYEYL CAWAYEVYDT DEHPALFQSP RDAFAAGMAI GGSRVHRMIP YDENFQIL T LPTTSEGKAK VQVGRGIKIN YIYYWSNSFR DPQIENTSVQ IRYDPFNIGI AYAFVRGQWV QCISQYYAEL QGRSEKELK LASIELRKHY SNHTQQSKVS AKNLAEFLAS VEAQEALLEQ RSYDAEVKEV FRVIEGGKAT TSRNEEPKLI QVTFANTDFQ ADEDEAIVP ETLVVYEEF

UniProtKB: Integrase

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Macromolecule #9: TnsC

MacromoleculeName: TnsC / type: protein_or_peptide / ID: 9 / Number of copies: 9 / Enantiomer: LEVO
Source (natural)Organism: Peltigera membranacea (fungus)
Molecular weightTheoretical: 43.521934 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MTKSTGFPLE LLTRPATERL AYFENYTVAH PRLKEVYEIL MRTIAEPAGA SFIFVYGASG VGKTTLRLRV EQKLTELALP KLESDRARV PVVGIEAIAP ESRYFNWKEY YTRALITLEE PLIDHKFDYG VRGISRDNFG KINVESKVVA PALRRALENA L IHRHPDVF ...String:
MTKSTGFPLE LLTRPATERL AYFENYTVAH PRLKEVYEIL MRTIAEPAGA SFIFVYGASG VGKTTLRLRV EQKLTELALP KLESDRARV PVVGIEAIAP ESRYFNWKEY YTRALITLEE PLIDHKFDYG VRGISRDNFG KINVESKVVA PALRRALENA L IHRHPDVF FVDEAQHFGK VASGYKLQDQ LDCLKSLANM TGILHCLLGT YELLTFRNLS GQLSRRSVDI HFRRYCADSP ED VQAFKSV LLTFQQHLPL AETPNLVDHW EYFYERTLGC IGTLKDWLKR VLSDALDREA TTITLKDLQK RALSVAQCQK MFK EIQEGE RQLSETEADV QNLRSALGLG AKPIVLPEET PKTTRPPGKV GKRKPKRDPI GVQQDVS

UniProtKB: ORC1/DEAH AAA+ ATPase domain-containing protein

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Macromolecule #10: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 10 / Number of copies: 11 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #11: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 11 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #12: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 12 / Number of copies: 7 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #13: water

MacromoleculeName: water / type: ligand / ID: 13 / Number of copies: 7 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 54.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING ONLY
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.65 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 38326
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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