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データを開く
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基本情報
登録情報 | ![]() | |||||||||
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タイトル | In situ human Post-eEF1A-AT-P-E state 80S ribosome | |||||||||
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![]() | In situ / Ribosome | |||||||||
機能・相同性 | ![]() cytoplasmic side of lysosomal membrane / Eukaryotic Translation Elongation / eukaryotic translation elongation factor 1 complex / regulation of chaperone-mediated autophagy / host-mediated activation of viral genome replication / eukaryotic 80S initiation complex / negative regulation of protein neddylation / response to insecticide / regulation of translation involved in cellular response to UV / negative regulation of endoplasmic reticulum unfolded protein response ...cytoplasmic side of lysosomal membrane / Eukaryotic Translation Elongation / eukaryotic translation elongation factor 1 complex / regulation of chaperone-mediated autophagy / host-mediated activation of viral genome replication / eukaryotic 80S initiation complex / negative regulation of protein neddylation / response to insecticide / regulation of translation involved in cellular response to UV / negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / axial mesoderm development / negative regulation of formation of translation preinitiation complex / regulation of G1 to G0 transition / ribosomal protein import into nucleus / positive regulation of respiratory burst involved in inflammatory response / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of gastrulation / 90S preribosome assembly / protein tyrosine kinase inhibitor activity / protein-DNA complex disassembly / IRE1-RACK1-PP2A complex / positive regulation of endodeoxyribonuclease activity / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / nucleolus organization / positive regulation of Golgi to plasma membrane protein transport / TNFR1-mediated ceramide production / kinase activator activity / negative regulation of DNA repair / negative regulation of RNA splicing / GAIT complex / positive regulation of DNA damage response, signal transduction by p53 class mediator / supercoiled DNA binding / TORC2 complex binding / G1 to G0 transition / NF-kappaB complex / positive regulation of ubiquitin-protein transferase activity / cysteine-type endopeptidase activator activity involved in apoptotic process / oxidized purine DNA binding / negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide / ubiquitin-like protein conjugating enzyme binding / negative regulation of bicellular tight junction assembly / regulation of establishment of cell polarity / middle ear morphogenesis / negative regulation of phagocytosis / rRNA modification in the nucleus and cytosol / Formation of the ternary complex, and subsequently, the 43S complex / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / laminin receptor activity / negative regulation of ubiquitin protein ligase activity / cortical actin cytoskeleton / protein kinase A binding / ion channel inhibitor activity / pigmentation / Ribosomal scanning and start codon recognition / homeostatic process / Translation initiation complex formation / positive regulation of mitochondrial depolarization / positive regulation of T cell receptor signaling pathway / macrophage chemotaxis / fibroblast growth factor binding / negative regulation of Wnt signaling pathway / lung morphogenesis / monocyte chemotaxis / positive regulation of natural killer cell proliferation / positive regulation of activated T cell proliferation / negative regulation of translational frameshifting / Protein hydroxylation / TOR signaling / BH3 domain binding / SARS-CoV-1 modulates host translation machinery / regulation of cell division / cellular response to ethanol / mTORC1-mediated signalling / regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / iron-sulfur cluster binding / Peptide chain elongation / Selenocysteine synthesis / Formation of a pool of free 40S subunits / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Eukaryotic Translation Termination / ubiquitin ligase inhibitor activity / blastocyst development / positive regulation of GTPase activity / cellular response to actinomycin D / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / translational elongation / negative regulation of ubiquitin-dependent protein catabolic process / positive regulation of signal transduction by p53 class mediator / protein serine/threonine kinase inhibitor activity / Viral mRNA Translation / negative regulation of respiratory burst involved in inflammatory response / Maturation of protein E / Maturation of protein E / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / protein localization to nucleus 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.8 Å | |||||||||
![]() | Wei Z / Yong X | |||||||||
資金援助 | 1件
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![]() | ![]() タイトル: In situ human Post-eEF1A-AT-P-E state 80S ribosome 著者: Wei Z / Yong X | |||||||||
履歴 |
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 483.4 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 101.8 KB 101.8 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 16.8 KB | 表示 | ![]() |
画像 | ![]() | 102 KB | ||
Filedesc metadata | ![]() | 19 KB | ||
その他 | ![]() ![]() | 475.3 MB 475.3 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 1.2 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1.2 MB | 表示 | |
XML形式データ | ![]() | 26.5 KB | 表示 | |
CIF形式データ | ![]() | 35.2 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 9azsMC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.068 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : In situ human Post-eEF1A-AT-P-E state 80S ribosome
+超分子 #1: In situ human Post-eEF1A-AT-P-E state 80S ribosome
+分子 #1: 60S ribosomal protein L19
+分子 #2: Small ribosomal subunit protein eS4, X isoform
+分子 #3: 40S ribosomal protein S8
+分子 #4: 40S ribosomal protein S11
+分子 #5: 40S ribosomal protein S23
+分子 #6: 40S ribosomal protein S6
+分子 #7: 40S ribosomal protein S9
+分子 #8: 40S ribosomal protein S24
+分子 #9: Small ribosomal subunit protein eS30
+分子 #10: 40S ribosomal protein SA
+分子 #11: 40S ribosomal protein S3a
+分子 #12: 40S ribosomal protein S7
+分子 #13: 40S ribosomal protein S21
+分子 #14: 40S ribosomal protein S26
+分子 #15: 40S ribosomal protein S2
+分子 #16: 40S ribosomal protein S13
+分子 #17: Small ribosomal subunit protein uS11
+分子 #18: 40S ribosomal protein S15a
+分子 #19: Small ribosomal subunit protein eS27
+分子 #23: 60S ribosomal protein L8
+分子 #24: Large ribosomal subunit protein uL3
+分子 #25: 60S ribosomal protein L4
+分子 #26: Large ribosomal subunit protein uL18
+分子 #27: 60S ribosomal protein L6
+分子 #28: 60S ribosomal protein L7
+分子 #29: 60S ribosomal protein L7a
+分子 #30: 60S ribosomal protein L9
+分子 #31: 60S ribosomal protein L10-like
+分子 #32: 60S ribosomal protein L11
+分子 #33: Large ribosomal subunit protein eL13
+分子 #34: 60S ribosomal protein L14
+分子 #35: 60S ribosomal protein L15
+分子 #36: 60S ribosomal protein L13a
+分子 #37: 60S ribosomal protein L17
+分子 #38: 60S ribosomal protein L18
+分子 #39: 60S ribosomal protein L18a
+分子 #40: 60S ribosomal protein L21
+分子 #41: Heparin-binding protein HBp15
+分子 #42: 60S ribosomal protein L23
+分子 #43: 60S ribosomal protein L23a
+分子 #44: 60S ribosomal protein L26
+分子 #45: 60S ribosomal protein L27
+分子 #46: 60S ribosomal protein L27a
+分子 #47: 60S ribosomal protein L29
+分子 #48: 60S ribosomal protein L30
+分子 #49: 60S ribosomal protein L31
+分子 #50: 60S ribosomal protein L32
+分子 #51: 60S ribosomal protein L35a
+分子 #52: 60S ribosomal protein L34
+分子 #53: 60S ribosomal protein L35
+分子 #54: 60S ribosomal protein L36
+分子 #55: 60S ribosomal protein L37
+分子 #56: 60S ribosomal protein L38
+分子 #57: 60S ribosomal protein L39
+分子 #58: Large ribosomal subunit protein eL40
+分子 #59: 60S ribosomal protein L41
+分子 #60: 60S ribosomal protein L36a
+分子 #61: 60S ribosomal protein L37a
+分子 #62: 60S ribosomal protein L28
+分子 #63: 40S ribosomal protein S17
+分子 #64: Small ribosomal subunit protein uS3
+分子 #65: 40S ribosomal protein S5
+分子 #66: 40S ribosomal protein S10
+分子 #67: Small ribosomal subunit protein uS19
+分子 #68: Small ribosomal subunit protein uS9
+分子 #69: 40S ribosomal protein S18
+分子 #70: 40S ribosomal protein S19
+分子 #71: 40S ribosomal protein S20
+分子 #72: 40S ribosomal protein S28
+分子 #73: 40S ribosomal protein S29
+分子 #74: Receptor of activated protein C kinase 1
+分子 #75: Small ribosomal subunit protein eS12
+分子 #76: Small ribosomal subunit protein eS25
+分子 #77: Ubiquitin-40S ribosomal protein S27a
+分子 #81: 60S ribosomal protein L12
+分子 #82: 60S ribosomal protein L10a
+分子 #83: 60S acidic ribosomal protein P0
+分子 #84: 60S ribosomal protein L24
+分子 #86: Elongation factor 1-alpha 1
+分子 #20: 28S rRNA
+分子 #21: 5S rRNA
+分子 #22: 5.8S rRNA
+分子 #78: 18S rRNA
+分子 #79: E site tRNA
+分子 #80: P site tRNA
+分子 #85: A/T site tRNA
+分子 #87: ZINC ION
+分子 #88: MAGNESIUM ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | cell |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 1.2 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
初期モデル | モデルのタイプ: EMDB MAP EMDB ID: 詳細: EMD15113 |
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最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 2.8 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 48914 |
初期 角度割当 | タイプ: ANGULAR RECONSTITUTION |
最終 角度割当 | タイプ: ANGULAR RECONSTITUTION |