+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-43826 | |||||||||
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Title | Tau(291-407)-4E THF | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Tau / amyloid fibrils / PHF / QHF / STRUCTURAL PROTEIN | |||||||||
Biological species | Escherichia coli (E. coli) | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 4.5 Å | |||||||||
Authors | El Mammeri N / Duan P / Hong M | |||||||||
Funding support | United States, 1 items
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Citation | Journal: To Be Published Title: Structure of THF Tau(297-407)-4E. Authors: El Mammeri N / Duan P / Hong M | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_43826.map.gz | 10.8 MB | EMDB map data format | |
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Header (meta data) | emd-43826-v30.xml emd-43826.xml | 13.3 KB 13.3 KB | Display Display | EMDB header |
Images | emd_43826.png | 48.8 KB | ||
Masks | emd_43826_msk_1.map | 216 MB | Mask map | |
Filedesc metadata | emd-43826.cif.gz | 3.7 KB | ||
Others | emd_43826_additional_1.map.gz emd_43826_half_map_1.map.gz emd_43826_half_map_2.map.gz | 170.9 MB 171.2 MB 171.2 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-43826 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-43826 | HTTPS FTP |
-Validation report
Summary document | emd_43826_validation.pdf.gz | 886.5 KB | Display | EMDB validaton report |
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Full document | emd_43826_full_validation.pdf.gz | 886 KB | Display | |
Data in XML | emd_43826_validation.xml.gz | 15.6 KB | Display | |
Data in CIF | emd_43826_validation.cif.gz | 18.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-43826 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-43826 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_43826.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_43826_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Additional map: #1
File | emd_43826_additional_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_43826_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_43826_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Tau(297-407)-4E
Entire | Name: Tau(297-407)-4E |
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Components |
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-Supramolecule #1: Tau(297-407)-4E
Supramolecule | Name: Tau(297-407)-4E / type: cell / ID: 1 / Parent: 0 / Details: tau construct with phospho-mimetic mutations |
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Source (natural) | Organism: Escherichia coli (E. coli) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | helical reconstruction |
Aggregation state | filament |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV |
-Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 1.033 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.2 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Applied symmetry - Helical parameters - Δz: 4.8 Å Applied symmetry - Helical parameters - Δ&Phi: -0.8 ° Applied symmetry - Helical parameters - Axial symmetry: C1 (asymmetric) Resolution.type: BY AUTHOR / Resolution: 4.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 85048 |
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Startup model | Type of model: NONE |
Final angle assignment | Type: NOT APPLICABLE |
-Atomic model buiding 1
Refinement | Protocol: AB INITIO MODEL |
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