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- EMDB-43826: Tau(291-407)-4E THF -

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Basic information

Entry
Database: EMDB / ID: EMD-43826
TitleTau(291-407)-4E THF
Map data
Sample
  • Cell: Tau(297-407)-4E
KeywordsTau / amyloid fibrils / PHF / QHF / STRUCTURAL PROTEIN
Biological speciesEscherichia coli (E. coli)
Methodhelical reconstruction / cryo EM / Resolution: 4.5 Å
AuthorsEl Mammeri N / Duan P / Hong M
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute on Aging (NIH/NIA)AG059661 United States
CitationJournal: To Be Published
Title: Structure of THF Tau(297-407)-4E.
Authors: El Mammeri N / Duan P / Hong M
History
DepositionFeb 26, 2024-
Header (metadata) releaseJun 5, 2024-
Map releaseJun 5, 2024-
UpdateJun 5, 2024-
Current statusJun 5, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_43826.png

Downloads & links

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Map

FileDownload / File: emd_43826.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
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AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 384 pix.
= 407.04 Å		1.06 Å/pix.
x 384 pix.
= 407.04 Å		1.06 Å/pix.
x 384 pix.
= 407.04 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.06 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.003
Minimum - Maximum-0.0054953014 - 0.011118108
Average (Standard dev.)0.00003546283 (±0.00038750796)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 407.03998 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_43826_msk_1.map
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AxesZYX

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Additional map: #1

Fileemd_43826_additional_1.map
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Half map: #1

Fileemd_43826_half_map_1.map
Projections & Slices
AxesZYX

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Half map: #2

Fileemd_43826_half_map_2.map
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Sample components

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Entire : Tau(297-407)-4E

EntireName: Tau(297-407)-4E
Components
  • Cell: Tau(297-407)-4E

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Supramolecule #1: Tau(297-407)-4E

SupramoleculeName: Tau(297-407)-4E / type: cell / ID: 1 / Parent: 0 / Details: tau construct with phospho-mimetic mutations
Source (natural)Organism: Escherichia coli (E. coli)

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Experimental details

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Structure determination

Methodcryo EM
Processinghelical reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 1.033 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Helical parameters - Δz: 4.8 Å
Applied symmetry - Helical parameters - Δ&Phi: -0.8 °
Applied symmetry - Helical parameters - Axial symmetry: C1 (asymmetric)
Resolution.type: BY AUTHOR / Resolution: 4.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 85048
Startup modelType of model: NONE
Final angle assignmentType: NOT APPLICABLE

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Atomic model buiding 1

RefinementProtocol: AB INITIO MODEL

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