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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | CryoET of VSV incubated with liposomes at pH 5.5 | |||||||||
![]() | Tomogram TS3016 from the 90s post-incubation dataset, pertaining to Fig. 2A. | |||||||||
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![]() | Virus / Liposome / Fusion / Class III Fusion / VSV / VSV G | |||||||||
Biological species | ![]() | |||||||||
Method | electron tomography / cryo EM | |||||||||
![]() | Si Z / Xia X / Tang S / Milojevic L | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Capturing Intermediates and Membrane Remodeling during Class III Fusion Authors: Milojevic L / Si Z / Xia X / Chen L / He Y / Tang S / Luo M / Zhou ZH | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 747.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 10.4 KB 10.4 KB | Display Display | ![]() |
Images | ![]() | 192.3 KB | ||
Filedesc metadata | ![]() | 4 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 394.5 KB | Display | ![]() |
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Full document | ![]() | 394.1 KB | Display | |
Data in XML | ![]() | 4.7 KB | Display | |
Data in CIF | ![]() | 5.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Tomogram TS3016 from the 90s post-incubation dataset, pertaining to Fig. 2A. | ||||||||||||||||||||
Voxel size | X=Y=Z: 8.16 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Vesicular stomatitis Indiana virus strain San Juan
Entire | Name: ![]() |
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Components |
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-Supramolecule #1: Vesicular stomatitis Indiana virus strain San Juan
Supramolecule | Name: Vesicular stomatitis Indiana virus strain San Juan / type: virus / ID: 1 / Parent: 0 / NCBI-ID: 11285 Sci species name: Vesicular stomatitis Indiana virus strain San Juan Virus type: VIRION / Virus isolate: OTHER / Virus enveloped: Yes / Virus empty: No |
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-Experimental details
-Structure determination
Method | cryo EM |
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![]() | electron tomography |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 5.5 |
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Grid | Model: PELCO Ultrathin Carbon with Lacey Carbon / Support film - Material: CARBON / Support film - topology: LACEY |
Vitrification | Cryogen name: ETHANE |
Sectioning | Other: NO SECTIONING |
Fiducial marker | Manufacturer: Cell Microscopy Core, Department of Cell Biology, University Medical Center Utrecht Diameter: 10 nm |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 100.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Number images used: 36 |
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