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- EMDB-42882: Amylin 1 receptor bound to salmon calcitonin (Amy1R:sCT) reconstr... -
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Open data
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Basic information
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Title | Amylin 1 receptor bound to salmon calcitonin (Amy1R:sCT) reconstructed from cryo-EM datasets recorded using a rectangular aperture | |||||||||
![]() | Amylin 1 receptor bound to salmon calcitonin (Amy2R:sCT) reconstructed from a dataset recorded using a rectangular beam | |||||||||
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![]() | Human Amylin 1 Receptor in complex with Gs and salmon calcitonin peptide / MEMBRANE PROTEIN | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.8 Å | |||||||||
![]() | Brown HG / Hanssen E | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Square condenser apertures for square cameras in low-dose transmission electron microscopy. Authors: Hamish G Brown / Dan Smith / Benjamin C Wardle / Eric Hanssen / ![]() Abstract: In transmission electron microscopy (TEM), cameras are square or rectangular but beams are round so the circular lobes irradiate adjacent areas, precluding further neighboring acquisition for beam- ...In transmission electron microscopy (TEM), cameras are square or rectangular but beams are round so the circular lobes irradiate adjacent areas, precluding further neighboring acquisition for beam-sensitive samples. We present condenser aperture plates with square and rectangular shapes that improve the efficiency of area usage by 70% and enhance montage imaging for beam-sensitive specimens. We demonstrate the compatibility of these condenser aperture plates with high-resolution cryogenic TEM by reconstructing a 1.8-Å map of equine apo-ferritin. #1: ![]() Title: Fitting a square beam in a square camera: Novel condenser apertures for low-dose transmission electron microscopy Authors: Brown HG / Hanssen E | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 230.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.9 KB 15.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() ![]() | 13.2 KB 13.2 KB | Display Display | ![]() |
Images | ![]() | 108.1 KB | ||
Masks | ![]() | 244.1 MB | ![]() | |
Filedesc metadata | ![]() | 4.6 KB | ||
Others | ![]() ![]() | 226.8 MB 226.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 21.3 KB | Display | |
Data in CIF | ![]() | 26.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Amylin 1 receptor bound to salmon calcitonin (Amy2R:sCT) reconstructed from a dataset recorded using a rectangular beam | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.833 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Density Histograms |
-Half map: Half map B
File | emd_42882_half_map_1.map | ||||||||||||
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Annotation | Half map B | ||||||||||||
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Density Histograms |
-Half map: Half map A
File | emd_42882_half_map_2.map | ||||||||||||
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Annotation | Half map A | ||||||||||||
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Density Histograms |
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Sample components
-Entire : Human Amylin1 Receptor in complex with Gs and salmon calcitonin p...
Entire | Name: Human Amylin1 Receptor in complex with Gs and salmon calcitonin peptide |
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Components |
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-Supramolecule #1: Human Amylin1 Receptor in complex with Gs and salmon calcitonin p...
Supramolecule | Name: Human Amylin1 Receptor in complex with Gs and salmon calcitonin peptide type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 189 KDa |
-Supramolecule #2: Receptor activity-modifying protein 1
Supramolecule | Name: Receptor activity-modifying protein 1 / type: complex / ID: 2 / Parent: 1 |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 4 mg/mL |
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Buffer | pH: 7.4 |
Grid | Material: GOLD / Support film - Material: GOLD / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Energy filter - Name: GIF Bioquantum / Energy filter - Slit width: 10 eV |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number real images: 3333 / Average electron dose: 58.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Calibrated defocus max: 1.5 µm / Calibrated defocus min: 0.5 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.4000000000000001 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 105000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |