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| タイトル | Square condenser apertures for square cameras in low-dose transmission electron microscopy. |
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| ジャーナル・号・ページ | Nat Methods, Vol. 21, Issue 4, Page 566-568, Year 2024 |
| 掲載日 | 2024年3月8日 |
著者 | Hamish G Brown / Dan Smith / Benjamin C Wardle / Eric Hanssen / ![]() |
| PubMed 要旨 | In transmission electron microscopy (TEM), cameras are square or rectangular but beams are round so the circular lobes irradiate adjacent areas, precluding further neighboring acquisition for beam- ...In transmission electron microscopy (TEM), cameras are square or rectangular but beams are round so the circular lobes irradiate adjacent areas, precluding further neighboring acquisition for beam-sensitive samples. We present condenser aperture plates with square and rectangular shapes that improve the efficiency of area usage by 70% and enhance montage imaging for beam-sensitive specimens. We demonstrate the compatibility of these condenser aperture plates with high-resolution cryogenic TEM by reconstructing a 1.8-Å map of equine apo-ferritin. |
リンク | Nat Methods / PubMed:38459386 |
| 手法 | EM (単粒子) |
| 解像度 | 1.76 - 2.8 Å |
| 構造データ | ![]() EMDB-41933: CryoEM map of horse spleen apoferritin determined as a reference for benchmarking square and rectangular apertures for cryo-EM ![]() EMDB-41936: CryoEM map of horse spleen apoferritin determined as a reference for benchmarking square and rectangular apertures for cryo-EM (Falcon IV round beam) ![]() EMDB-41937: CryoEM map of horse spleen apoferritin determined for benchmarking square and rectangular apertures for cryo-EM (Falcon IV square beam) ![]() EMDB-41938: CryoEM map of horse spleen apoferritin determined for benchmarking square and rectangular apertures for cryo-EM (Gatan K3 rectangular beam) ![]() EMDB-42882: Amylin 1 receptor bound to salmon calcitonin (Amy1R:sCT) reconstructed from cryo-EM datasets recorded using a rectangular aperture |
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Homo sapiens (ヒト)