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Open data
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Basic information
Entry | ![]() | ||||||||||||
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Title | mGluR3 class 1 in the presence of the antagonist LY 341495 | ||||||||||||
![]() | Full length map | ||||||||||||
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![]() | GPCR / synaptic protein / MEMBRANE PROTEIN | ||||||||||||
Function / homology | ![]() Class C/3 (Metabotropic glutamate/pheromone receptors) / group II metabotropic glutamate receptor activity / G protein-coupled glutamate receptor signaling pathway / astrocyte projection / G alpha (i) signalling events / postsynaptic modulation of chemical synaptic transmission / calcium channel regulator activity / regulation of synaptic transmission, glutamatergic / sensory perception of pain / modulation of chemical synaptic transmission ...Class C/3 (Metabotropic glutamate/pheromone receptors) / group II metabotropic glutamate receptor activity / G protein-coupled glutamate receptor signaling pathway / astrocyte projection / G alpha (i) signalling events / postsynaptic modulation of chemical synaptic transmission / calcium channel regulator activity / regulation of synaptic transmission, glutamatergic / sensory perception of pain / modulation of chemical synaptic transmission / presynaptic membrane / gene expression / scaffold protein binding / postsynapse / postsynaptic membrane / dendritic spine / postsynaptic density / neuron projection / axon / glutamatergic synapse / plasma membrane Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.4 Å | ||||||||||||
![]() | Strauss A / Levitz J | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis of allosteric modulation of metabotropic glutamate receptor activation and desensitization Authors: Strauss A | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 137.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 28.3 KB 28.3 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 13.7 KB | Display | ![]() |
Images | ![]() | 45.9 KB | ||
Filedesc metadata | ![]() | 6.5 KB | ||
Others | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | 253.2 MB 253.2 MB 208.7 MB 207.9 MB 132.7 MB 128 MB 253.4 MB 253.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 942.5 KB | Display | ![]() |
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Full document | ![]() | 942.1 KB | Display | |
Data in XML | ![]() | 22.9 KB | Display | |
Data in CIF | ![]() | 29.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8trdMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Full length map | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.825 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: Half map A for locally refined map of the extracellular domain
File | emd_41578_additional_1.map | ||||||||||||
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Annotation | Half map A for locally refined map of the extracellular domain | ||||||||||||
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-Additional map: Half map B for locally refined map of the extracellular domain
File | emd_41578_additional_2.map | ||||||||||||
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Annotation | Half map B for locally refined map of the extracellular domain | ||||||||||||
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-Additional map: Half map A of the transmembrane domain following particle subtraction
File | emd_41578_additional_3.map | ||||||||||||
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Annotation | Half map A of the transmembrane domain following particle subtraction | ||||||||||||
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-Additional map: Half map B of the transmembrane domain following particle subtraction
File | emd_41578_additional_4.map | ||||||||||||
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Annotation | Half map B of the transmembrane domain following particle subtraction | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: Locally refined map of the extracellular domain
File | emd_41578_additional_5.map | ||||||||||||
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Annotation | Locally refined map of the extracellular domain | ||||||||||||
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Density Histograms |
-Additional map: Map of the transmembrane domain following particle subtraction
File | emd_41578_additional_6.map | ||||||||||||
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Annotation | Map of the transmembrane domain following particle subtraction | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map B of full length map
File | emd_41578_half_map_1.map | ||||||||||||
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Annotation | Half map B of full length map | ||||||||||||
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-Half map: Half map A of full length map
File | emd_41578_half_map_2.map | ||||||||||||
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Annotation | Half map A of full length map | ||||||||||||
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Sample components
-Entire : Metabotropic Glutamate Receptor 3 dimer
Entire | Name: Metabotropic Glutamate Receptor 3 dimer |
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Components |
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-Supramolecule #1: Metabotropic Glutamate Receptor 3 dimer
Supramolecule | Name: Metabotropic Glutamate Receptor 3 dimer / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 260 KDa |
-Macromolecule #1: Metabotropic glutamate receptor 3
Macromolecule | Name: Metabotropic glutamate receptor 3 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 103.161555 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MKMLTRLQIL MLALFSKGFL LSLGDHNFMR REIKIEGDLV LGGLFPINEK GTGTEECGRI NEDRGIQRLE AMLFAIDEIN KDNYLLPGV KLGVHILDTC SRDTYALEQS LEFVRASLTK VDEAEYMCPD GSYAIQENIP LLIAGVIGGS YSSVSIQVAN L LRLFQIPQ ...String: MKMLTRLQIL MLALFSKGFL LSLGDHNFMR REIKIEGDLV LGGLFPINEK GTGTEECGRI NEDRGIQRLE AMLFAIDEIN KDNYLLPGV KLGVHILDTC SRDTYALEQS LEFVRASLTK VDEAEYMCPD GSYAIQENIP LLIAGVIGGS YSSVSIQVAN L LRLFQIPQ ISYASTSAKL SDKSRYDYFA RTVPPDFYQA KAMAEILRFF NWTYVSTVAS EGDYGETGIE AFEQEARLRN IC IATAEKV GRSNIRKSYD SVIRELLQKP NARVVVLFMR SDDSRELIAA ANRVNASFTW VASDGWGAQE SIVKGSEHVA YGA ITLELA SHPVRQFDRY FQSLNPYNNH RNPWFRDFWE QKFQCSLQNK RNHRQVCDKH LAIDSSNYEQ ESKIMFVVNA VYAM AHALH KMQRTLCPNT TKLCDAMKIL DGKKLYKEYL LKINFTAPFN PNKGADSIVK FDTFGDGMGR YNVFNLQQTG GKYSY LKVG HWAETLSLDV DSIHWSRNSV PTSQCSDPCA PNEMKNMQPG DVCCWICIPC EPYEYLVDEF TCMDCGPGQW PTADLS GCY NLPEDYIKWE DAWAIGPVTI ACLGFLCTCI VITVFIKHNN TPLVKASGRE LCYILLFGVS LSYCMTFFFI AKPSPVI CA LRRLGLGTSF AICYSALLTK TNCIARIFDG VKNGAQRPKF ISPSSQVFIC LGLILVQIVM VSVWLILETP GTRRYTLP E KRETVILKCN VKDSSMLISL TYDVVLVILC TVYAFKTRKC PENFNEAKFI GFTMYTTCII WLAFLPIFYV TSSDYRVQT TTMCISVSLS GFVVLGCLFA PKVHIVLFQP QKNVVTHRLH LNRFSVSGTA TTYSQSSAST YVPTVCNGRE VLDSTTSSLA GLVPRGSAA AKSAWSHPQF EKGGGSGGGS GGGSWSHPQF EK UniProtKB: Metabotropic glutamate receptor 3 |
-Macromolecule #2: 2-[(1S,2S)-2-carboxycyclopropyl]-3-(9H-xanthen-9-yl)-D-alanine
Macromolecule | Name: 2-[(1S,2S)-2-carboxycyclopropyl]-3-(9H-xanthen-9-yl)-D-alanine type: ligand / ID: 2 / Number of copies: 2 / Formula: Z99 |
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Molecular weight | Theoretical: 353.369 Da |
Chemical component information | ![]() ChemComp-Z99: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 4.5 mg/mL |
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Buffer | pH: 7.5 |
Grid | Model: UltrAuFoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: GOLD / Support film - topology: HOLEY |
Vitrification | Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Detector mode: COUNTING / Average electron dose: 58.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.7000000000000001 µm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Space: REAL / Protocol: AB INITIO MODEL |
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Output model | ![]() PDB-8trd: |