- EMDB-41523: Polyclonal immune complex of Fab binding the H2 HA from serum of ... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-41523
タイトル
Polyclonal immune complex of Fab binding the H2 HA from serum of subject 2-1 at week 4
マップデータ
Negative stain map of global refinement of particle stack used to sort polyclonal responses to H2 HA from human subject 2-1 at week 4Negative stain map of half map A for global refinement of particle
試料
複合体: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 2-1 at week 4
キーワード
influenza / hemagglutinin / H2 / polyclonal complex / Fab complex / viral fusion protein / VIRAL PROTEIN
ジャーナル: bioRxiv / 年: 2023 タイトル: Immune memory shapes human polyclonal antibody responses to H2N2 vaccination. 著者: Yuhe R Yang / Julianna Han / Hailee R Perrett / Sara T Richey / Abigail M Jackson / Alesandra J Rodriguez / Rebecca A Gillespie / Sarah O'Connell / Julie E Raab / Lauren Y Cominsky / Ankita ...著者: Yuhe R Yang / Julianna Han / Hailee R Perrett / Sara T Richey / Abigail M Jackson / Alesandra J Rodriguez / Rebecca A Gillespie / Sarah O'Connell / Julie E Raab / Lauren Y Cominsky / Ankita Chopde / Masaru Kanekiyo / Katherine V Houser / Grace L Chen / Adrian B McDermott / Sarah F Andrews / Andrew B Ward / 要旨: Influenza A virus subtype H2N2, which caused the 1957 influenza pandemic, remains a global threat. A recent phase I clinical trial investigating a ferritin nanoparticle displaying H2 hemagglutinin in ...Influenza A virus subtype H2N2, which caused the 1957 influenza pandemic, remains a global threat. A recent phase I clinical trial investigating a ferritin nanoparticle displaying H2 hemagglutinin in H2-naïve and H2-exposed adults. Therefore, we could perform comprehensive structural and biochemical characterization of immune memory on the breadth and diversity of the polyclonal serum antibody response elicited after H2 vaccination. We temporally map the epitopes targeted by serum antibodies after first and second vaccinations and show previous H2 exposure results in higher responses to the variable head domain of hemagglutinin while initial responses in H2-naïve participants are dominated by antibodies targeting conserved epitopes. We use cryo-EM and monoclonal B cell isolation to describe the molecular details of cross-reactive antibodies targeting conserved epitopes on the hemagglutinin head including the receptor binding site and a new site of vulnerability deemed the medial junction. Our findings accentuate the impact of pre-existing influenza exposure on serum antibody responses.
ダウンロード / ファイル: emd_41523.map.gz / 形式: CCP4 / 大きさ: 15.6 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Negative stain map of global refinement of particle stack used to sort polyclonal responses to H2 HA from human subject 2-1 at week 4Negative stain map of half map A for global refinement of particle
ボクセルのサイズ
X=Y=Z: 1.77 Å
密度
表面レベル
登録者による: 0.0106
最小 - 最大
-0.026874403 - 0.06304216
平均 (標準偏差)
0.000400749 (±0.0045486027)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
160
160
160
Spacing
160
160
160
セル
A=B=C: 283.2 Å α=β=γ: 90.0 °
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添付データ
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追加マップ: Negative stain map of polyclonal Fab binding the...