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- EMDB-41515: Polyclonal immune complex of Fab binding the H2 HA from serum of ... -
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Open data
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Basic information
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Title | Polyclonal immune complex of Fab binding the H2 HA from serum of subject 1-1 at week 4 | |||||||||
![]() | Negative stain map of global refinement of particle stack used to sort polyclonal responses to H2 HA from human subject 1-1 at week 4 | |||||||||
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![]() | influenza / hemagglutinin / H2 / polyclonal complex / Fab complex / viral fusion protein / VIRAL PROTEIN | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / negative staining / Resolution: 20.0 Å | |||||||||
![]() | Yang YR / Han J / Richey ST / Ward AB | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Immune memory shapes human polyclonal antibody responses to H2N2 vaccination. Authors: Yuhe R Yang / Julianna Han / Hailee R Perrett / Sara T Richey / Alesandra J Rodriguez / Abigail M Jackson / Rebecca A Gillespie / Sarah O'Connell / Julie E Raab / Lauren Y Cominsky / Ankita ...Authors: Yuhe R Yang / Julianna Han / Hailee R Perrett / Sara T Richey / Alesandra J Rodriguez / Abigail M Jackson / Rebecca A Gillespie / Sarah O'Connell / Julie E Raab / Lauren Y Cominsky / Ankita Chopde / Masaru Kanekiyo / Katherine V Houser / Grace L Chen / Adrian B McDermott / Sarah F Andrews / Andrew B Ward / ![]() ![]() Abstract: Influenza A virus subtype H2N2, which caused the 1957 influenza pandemic, remains a global threat. A recent phase 1 clinical trial investigating a ferritin nanoparticle vaccine displaying H2 ...Influenza A virus subtype H2N2, which caused the 1957 influenza pandemic, remains a global threat. A recent phase 1 clinical trial investigating a ferritin nanoparticle vaccine displaying H2 hemagglutinin (HA) in H2-naive and H2-exposed adults enabled us to perform comprehensive structural and biochemical characterization of immune memory on the breadth and diversity of the polyclonal serum antibody response elicited. We temporally map the epitopes targeted by serum antibodies after vaccine prime and boost, revealing that previous H2 exposure results in higher responses to the variable HA head domain. In contrast, initial responses in H2-naive participants are dominated by antibodies targeting conserved epitopes. We use cryoelectron microscopy and monoclonal B cell isolation to describe the molecular details of cross-reactive antibodies targeting conserved epitopes on the HA head, including the receptor-binding site and a new site of vulnerability deemed the medial junction. Our findings accentuate the impact of pre-existing influenza exposure on serum antibody responses post-vaccination. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 11.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 20.7 KB 20.7 KB | Display Display | ![]() |
Images | ![]() | 37.8 KB | ||
Filedesc metadata | ![]() | 4.3 KB | ||
Others | ![]() ![]() ![]() ![]() ![]() | 11.9 MB 11.9 MB 11.9 MB 11.9 MB 11.9 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | C: citing same article ( |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Negative stain map of global refinement of particle stack used to sort polyclonal responses to H2 HA from human subject 1-1 at week 4 | ||||||||||||||||||||
Voxel size | X=Y=Z: 2.06 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: Negative stain map of polyclonal Fab binding the...
File | emd_41515_additional_1.map | ||||||||||||
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Annotation | Negative stain map of polyclonal Fab binding the H2 HA RBS epitope from human subject 1-1 at week 4 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: Negative stain map of polyclonal Fab binding the...
File | emd_41515_additional_2.map | ||||||||||||
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Annotation | Negative stain map of polyclonal Fab binding the H2 HA central stem epitope from human subject 1-1 at week 4 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: Negative stain map of polyclonal Fab binding the...
File | emd_41515_additional_3.map | ||||||||||||
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Annotation | Negative stain map of polyclonal Fab binding the H2 HA anchor epitope from human subject 1-1 at week 4 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Negative stain map of half map A for...
File | emd_41515_half_map_1.map | ||||||||||||
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Annotation | Negative stain map of half map A for global refinement of particle stack used to sort polyclonal responses to H2 HA from human subject 1-1 at week 4 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Negative stain map of half map B for...
File | emd_41515_half_map_2.map | ||||||||||||
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Annotation | Negative stain map of half map B for global refinement of particle stack used to sort polyclonal responses to H2 HA from human subject 1-1 at week 4 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Polyclonal immune complex of Fab binding the H2 HA from serum of ...
Entire | Name: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 1-1 at week 4 |
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Components |
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-Supramolecule #1: Polyclonal immune complex of Fab binding the H2 HA from serum of ...
Supramolecule | Name: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 1-1 at week 4 type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 280 KDa |
-Experimental details
-Structure determination
Method | negative staining |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.015 mg/mL |
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Buffer | pH: 7.4 |
Staining | Type: NEGATIVE / Material: 2% w/v uranyl formate |
Grid | Model: Homemade / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Pretreatment - Type: GLOW DISCHARGE |
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Electron microscopy
Microscope | FEI TECNAI SPIRIT |
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Image recording | Film or detector model: FEI EAGLE (4k x 4k) / Average electron dose: 25.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: LAB6 |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: DARK FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 2.0 µm |
Sample stage | Specimen holder model: SIDE ENTRY, EUCENTRIC |
Experimental equipment | ![]() Model: Tecnai Spirit / Image courtesy: FEI Company |