National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
NIH R01 GM45377
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
NIH P41 GM109824
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
NIH R01 GM112108
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
NIH GM117212
米国
引用
ジャーナル: Mol Cell / 年: 2023 タイトル: Implications of a multiscale structure of the yeast nuclear pore complex. 著者: Christopher W Akey / Ignacia Echeverria / Christna Ouch / Ilona Nudelman / Yi Shi / Junjie Wang / Brian T Chait / Andrej Sali / Javier Fernandez-Martinez / Michael P Rout / 要旨: Nuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules. Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D density maps from ...Nuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules. Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D density maps from cryogenic electron microscopy and AlphaFold2 models. Key features of the inner and outer rings were integrated into a comprehensive model. We resolved flexible connectors that tie together the core scaffold, along with equatorial transmembrane complexes and a lumenal ring that anchor this channel within the pore membrane. The organization of the nuclear double outer ring reveals an architecture that may be shared with ancestral NPCs. Additional connections between the core scaffold and the central transporter suggest that under certain conditions, a degree of local organization is present at the periphery of the transport machinery. These connectors may couple conformational changes in the scaffold to the central transporter to modulate transport. Collectively, this analysis provides insights into assembly, transport, and NPC evolution.
超分子 #2: connectors in the cytoplasmic double outer ring
超分子
名称: connectors in the cytoplasmic double outer ring / タイプ: complex / ID: 2 / 親要素: 1 詳細: subset map from yeast NPCs showing connectors from single cytoplasmic outer ring to the central transport assembly
タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 3.0.7) / 詳細: C8 symmetry used
最終 角度割当
タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 3.0.7) 詳細: C8 symmetry used with appropriate mask to focus on single outer ring and part of the inner ring
最終 3次元分類
ソフトウェア - 名称: RELION (ver. 3.0.7)
FSC曲線 (解像度の算出)
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原子モデル構築 1
初期モデル
Chain - Source name: Other / Chain - Initial model type: experimental model / 詳細: none
詳細
Rigid body docking of 3D map of cytoplasmic ring with Chimera
精密化
空間: REAL / プロトコル: RIGID BODY FIT / 当てはまり具合の基準: cross correlation