National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
NIH R01 GM45377
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
NIH P41 GM109824
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
NIH R01 GM112108
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
NIH GM117212
米国
引用
ジャーナル: Mol Cell / 年: 2023 タイトル: Implications of a multiscale structure of the yeast nuclear pore complex. 著者: Christopher W Akey / Ignacia Echeverria / Christna Ouch / Ilona Nudelman / Yi Shi / Junjie Wang / Brian T Chait / Andrej Sali / Javier Fernandez-Martinez / Michael P Rout / 要旨: Nuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules. Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D density maps from ...Nuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules. Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D density maps from cryogenic electron microscopy and AlphaFold2 models. Key features of the inner and outer rings were integrated into a comprehensive model. We resolved flexible connectors that tie together the core scaffold, along with equatorial transmembrane complexes and a lumenal ring that anchor this channel within the pore membrane. The organization of the nuclear double outer ring reveals an architecture that may be shared with ancestral NPCs. Additional connections between the core scaffold and the central transporter suggest that under certain conditions, a degree of local organization is present at the periphery of the transport machinery. These connectors may couple conformational changes in the scaffold to the central transporter to modulate transport. Collectively, this analysis provides insights into assembly, transport, and NPC evolution.
モデルのタイプ: OTHER 詳細: C8 symmetrized NPC map segmented in CHIMERA with Segger to provide initial 3D map of the double outer ring, which was low pass filtered for the first reference.