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- EMDB-40894: Singlet A-tubule from Tetrahymena thermophila -

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Basic information

Entry
Database: EMDB / ID: EMD-40894
TitleSinglet A-tubule from Tetrahymena thermophila
Map data
Sample
  • Organelle or cellular component: Singlet A-tubule from Tetrahymena thermophila
KeywordsCilia / Axoneme / central pair / microtubules / STRUCTURAL PROTEIN
Biological speciesTetrahymena thermophila (eukaryote)
Methodsubtomogram averaging / cryo EM / Resolution: 15.1 Å
AuthorsLegal T / Bui KH
Funding support Canada, 1 items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR)PJT-156354 Canada
CitationJournal: J.Cell Biol. / Year: 2023
Title: CEP104/FAP256 and associated cap complex maintain stability of the ciliary tip.
Authors: Legal T / Parra M / Tong M / Black CS / Joachimiak E / Valente-Paterno M / Lechtreck K / Gaertig J / Bui KH
History
DepositionMay 26, 2023-
Header (metadata) releaseSep 13, 2023-
Map releaseSep 13, 2023-
UpdateOct 11, 2023-
Current statusOct 11, 2023Processing site: RCSB / Status: Released

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Structure visualization

Downloads & links

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Map

FileDownload / File: emd_40894.map.gz / Format: CCP4 / Size: 5.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 3.37 Å
Density
Contour LevelBy EMDB: 3.5
Minimum - Maximum-6.7943745 - 12.180952
Average (Standard dev.)0.06171023 (±2.714548)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions112112112
Spacing112112112
CellA=B=C: 377.44 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Singlet A-tubule from Tetrahymena thermophila

EntireName: Singlet A-tubule from Tetrahymena thermophila
Components
  • Organelle or cellular component: Singlet A-tubule from Tetrahymena thermophila

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Supramolecule #1: Singlet A-tubule from Tetrahymena thermophila

SupramoleculeName: Singlet A-tubule from Tetrahymena thermophila / type: organelle_or_cellular_component / ID: 1 / Parent: 0
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428 / Organelle: Cilia

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

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Sample preparation

Concentration1.8 mg/mL
BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 4.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 2.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 15.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 3459
ExtractionNumber tomograms: 5 / Number images used: 3459
Final angle assignmentType: MAXIMUM LIKELIHOOD

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