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- EMDB-40906: Ciliary tip central pair composite map from Tetrahymena thermophila -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Ciliary tip central pair composite map from Tetrahymena thermophila | |||||||||
![]() | Composite map made using: EMD-40897, EMD-40896, EMD-40898, EMD-40899, EMD-40900 | |||||||||
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![]() | Cilia / Axoneme / central pair / microtubules / STRUCTURAL PROTEIN | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 8.8 Å | |||||||||
![]() | Legal T / Bui KH | |||||||||
Funding support | ![]()
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![]() | ![]() Title: CEP104/FAP256 and associated cap complex maintain stability of the ciliary tip Authors: Legal T / Parra M / Tong M / Black CS / Joachimiak E / Valente-Paterno M / Lechtreck K / Gaertig J / Bui KH | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 16.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.1 KB 9.1 KB | Display Display | ![]() |
Images | ![]() | 133.3 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 367.3 KB | Display | ![]() |
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Full document | ![]() | 366.9 KB | Display | |
Data in XML | ![]() | 4.7 KB | Display | |
Data in CIF | ![]() | 5.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Composite map made using: EMD-40897, EMD-40896, EMD-40898, EMD-40899, EMD-40900 | ||||||||||||||||||||
Voxel size | X=Y=Z: 4.24 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : composite map obtained by subtomogram averaging of the ciliary ti...
Entire | Name: composite map obtained by subtomogram averaging of the ciliary tip central pair |
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Components |
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-Supramolecule #1: composite map obtained by subtomogram averaging of the ciliary ti...
Supramolecule | Name: composite map obtained by subtomogram averaging of the ciliary tip central pair type: organelle_or_cellular_component / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | cell |
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Sample preparation
Concentration | 1.8 mg/mL |
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Buffer | pH: 7.4 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 4.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 2.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 8.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 11534 |
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Extraction | Number tomograms: 144 / Number images used: 11980 |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |