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- EMDB-40587: CryoEM reconstruction of full-length Btk (class 3) -

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Basic information

Entry
Database: EMDB / ID: EMD-40587
TitleCryoEM reconstruction of full-length Btk (class 3)
Map datasharpened refined map
Sample
  • Complex: Full-length Bruton's Tyrosine Kinase
    • Protein or peptide: Full-length Bruton's Tyrosine Kinase
KeywordsBruton's Tyrosine Kinase / ATP-binding / protein phosphorylation / TRANSFERASE
Function / homology
Function and homology information


negative regulation of B cell activation / G beta:gamma signalling through BTK / RHO GTPases Activate WASPs and WAVEs / G alpha (q) signalling events / FCERI mediated Ca+2 mobilization / G alpha (12/13) signalling events / negative regulation of leukocyte proliferation / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Regulation of actin dynamics for phagocytic cup formation / proteoglycan catabolic process ...negative regulation of B cell activation / G beta:gamma signalling through BTK / RHO GTPases Activate WASPs and WAVEs / G alpha (q) signalling events / FCERI mediated Ca+2 mobilization / G alpha (12/13) signalling events / negative regulation of leukocyte proliferation / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Regulation of actin dynamics for phagocytic cup formation / proteoglycan catabolic process / monocyte proliferation / positive regulation of interleukin-17A production / eosinophil homeostasis / positive regulation of type III hypersensitivity / B cell affinity maturation / positive regulation of synoviocyte proliferation / histamine secretion by mast cell / neutrophil homeostasis / cellular response to molecule of fungal origin / positive regulation of type I hypersensitivity / cellular response to interleukin-7 / DAP12 signaling / negative regulation of cytokine production / positive regulation of immunoglobulin production / positive regulation of NLRP3 inflammasome complex assembly / phospholipase activator activity / negative regulation of interleukin-10 production / negative regulation of B cell proliferation / phosphatidylinositol-3,4,5-trisphosphate binding / phospholipase binding / positive regulation of phagocytosis / positive regulation of B cell proliferation / cell maturation / B cell receptor signaling pathway / non-specific protein-tyrosine kinase / non-membrane spanning protein tyrosine kinase activity / positive regulation of interleukin-6 production / cellular response to reactive oxygen species / positive regulation of tumor necrosis factor production / T cell receptor signaling pathway / protein tyrosine kinase activity / cytoplasmic vesicle / adaptive immune response / response to lipopolysaccharide / intracellular signal transduction / membrane raft / innate immune response / apoptotic process / perinuclear region of cytoplasm / ATP binding / identical protein binding / nucleus / metal ion binding / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Tyrosine-protein kinase BTK, SH3 domain / Zinc finger, Btk motif / BTK motif / Zinc finger Btk-type profile. / Bruton's tyrosine kinase Cys-rich motif / : / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain ...Tyrosine-protein kinase BTK, SH3 domain / Zinc finger, Btk motif / BTK motif / Zinc finger Btk-type profile. / Bruton's tyrosine kinase Cys-rich motif / : / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / Src homology 3 domains / SH2 domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / PH-like domain superfamily / Tyrosine-protein kinase, active site / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
Tyrosine-protein kinase BTK
Similarity search - Component
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.56 Å
AuthorsLin DY / Andreotti AH / Juneja P
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) United States
CitationJournal: Elife / Year: 2024
Title: Conformational heterogeneity of the BTK PHTH domain drives multiple regulatory states.
Authors: David Yin-Wei Lin / Lauren E Kueffer / Puneet Juneja / Thomas E Wales / John R Engen / Amy H Andreotti /
Abstract: Full-length Bruton's tyrosine kinase (BTK) has been refractory to structural analysis. The nearest full-length structure of BTK to date consists of the autoinhibited SH3-SH2-kinase core. Precisely ...Full-length Bruton's tyrosine kinase (BTK) has been refractory to structural analysis. The nearest full-length structure of BTK to date consists of the autoinhibited SH3-SH2-kinase core. Precisely how the BTK N-terminal domains (the Pleckstrin homology/Tec homology [PHTH] domain and proline-rich regions [PRR] contain linker) contribute to BTK regulation remains unclear. We have produced crystals of full-length BTK for the first time but despite efforts to stabilize the autoinhibited state, the diffraction data still reveal only the SH3-SH2-kinase core with no electron density visible for the PHTH-PRR segment. Cryo-electron microscopy (cryoEM) data of full-length BTK, on the other hand, provide the first view of the PHTH domain within full-length BTK. CryoEM reconstructions support conformational heterogeneity in the PHTH-PRR region wherein the globular PHTH domain adopts a range of states arrayed around the autoinhibited SH3-SH2-kinase core. On the way to activation, disassembly of the SH3-SH2-kinase core opens a new autoinhibitory site on the kinase domain for PHTH domain binding that is ultimately released upon interaction of PHTH with phosphatidylinositol (3,4,5)-trisphosphate. Membrane-induced dimerization activates BTK and we present here a crystal structure of an activation loop swapped BTK kinase domain dimer that likely represents the conformational state leading to trans-autophosphorylation. Together, these data provide the first structural elucidation of full-length BTK and allow a deeper understanding of allosteric control over the BTK kinase domain during distinct stages of activation.
History
DepositionApr 25, 2023-
Header (metadata) releaseAug 16, 2023-
Map releaseAug 16, 2023-
UpdateJan 31, 2024-
Current statusJan 31, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

Downloads & links

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Map

FileDownload / File: emd_40587.map.gz / Format: CCP4 / Size: 219.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationsharpened refined map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.45 Å/pix.
x 386 pix.
= 174.916 Å
0.45 Å/pix.
x 386 pix.
= 174.916 Å
0.45 Å/pix.
x 386 pix.
= 174.916 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.45315 Å
Density
Contour LevelBy AUTHOR: 0.04
Minimum - Maximum-0.51711774 - 0.6359175
Average (Standard dev.)0.00019444848 (±0.009745997)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions386386386
Spacing386386386
CellA=B=C: 174.91591 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: half map B

Fileemd_40587_half_map_1.map
Annotationhalf map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: half map A

Fileemd_40587_half_map_2.map
Annotationhalf map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire : Full-length Bruton's Tyrosine Kinase

EntireName: Full-length Bruton's Tyrosine Kinase
Components
  • Complex: Full-length Bruton's Tyrosine Kinase
    • Protein or peptide: Full-length Bruton's Tyrosine Kinase

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Supramolecule #1: Full-length Bruton's Tyrosine Kinase

SupramoleculeName: Full-length Bruton's Tyrosine Kinase / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mus musculus (house mouse)

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Macromolecule #1: Full-length Bruton's Tyrosine Kinase

MacromoleculeName: Full-length Bruton's Tyrosine Kinase / type: protein_or_peptide / ID: 1 / Details: Mouse full-length Btk with inhibitory mutations / Enantiomer: LEVO / EC number: non-specific protein-tyrosine kinase
Source (natural)Organism: Mus musculus (house mouse)
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MAAVILESIF LKRSQQKKKT SPLNFKKRLF LLTVHKLSYY EYDFERGRRG SKKGSIDVEK ITCVETVIP EKNPPPERQI PRRGEESSEM EQISIIERFP YPFQVVYDEG PLYVFSPTEE L RKRWIHQL KNVIRYNSDL VQKYHPCFWI DGQYLCCSQT AKNAMGCQIL ...String:
MAAVILESIF LKRSQQKKKT SPLNFKKRLF LLTVHKLSYY EYDFERGRRG SKKGSIDVEK ITCVETVIP EKNPPPERQI PRRGEESSEM EQISIIERFP YPFQVVYDEG PLYVFSPTEE L RKRWIHQL KNVIRYNSDL VQKYHPCFWI DGQYLCCSQT AKNAMGCQIL ENRNGSLKPG SS HRKTKKP LPPTPEEDQI LKKPLPPEPT AAPISTTELK KVVALYDYMP MNANDLQLRK GEE YFILEE SNLPWWRARD KNGQEGYIPS NYITEAEDSI EMYEWYSKHM TRSQAEQLLK QAGA AGGFI VRDSSKAGKY TVSVFAKSTG EPQGVIRHYV VCSTPQSQYY LAEKHLFSTI PELIN YHQH NSAGLISRLK YPVSKQNKNP PPPPGFGYGS WEIDPKDLTF LKELGTGQFG VVKYGK WRG QYDVAIRMIR EGSMSEDEFI EEAKVMMNLS HEKLVQLYGV CTKQRPIFII TEYMANG CL LNYLREMRHR FQTQQLLEMC KDVCEAMEYL ESKQFLHRDL AARNCLVNDQ GVVKVSDF G MTRYVLDDEY TSSTGSKFPV KWASPEVLMY SKFSSKSDIW AFGVLMWEIY SLGKMPYER FTNSETAEHI AQGLRLPRPH LASERVYTIM YSCWHEKADE RPSFKILLSN ILDVMDEES

UniProtKB: Tyrosine-protein kinase BTK

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.5 mg/mL
BufferpH: 8
Component:
ConcentrationName
20.0 mMTris(hydroxymethyl)aminomethane
150.0 mMsodium chloride
1.0 mMDithiothreitol
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 12 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 120 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
DetailsThe sample was monodisperse.

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Electron microscopy

MicroscopeTFS GLACIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Digitization - Dimensions - Width: 11520 pixel / Digitization - Dimensions - Height: 8184 pixel / Number grids imaged: 1 / Number real images: 755 / Average exposure time: 2.0 sec. / Average electron dose: 59.0 e/Å2
Details: Images were collected in Super-resolution made at 50 frames per second
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 45000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN

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Image processing

Particle selectionNumber selected: 2268597
Startup modelType of model: INSILICO MODEL
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 5.56 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.1.2) / Number images used: 22406
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.1.2)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.1.2)
Final 3D classificationNumber classes: 4 / Avg.num./class: 16000 / Software - Name: cryoSPARC (ver. 4.1.2)
FSC plot (resolution estimation)

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