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基本情報
登録情報 | ![]() | |||||||||
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タイトル | cryo-EM structure of PSII-LHCII megacomplex from spinach | |||||||||
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![]() | PSII / LHCII / megacomplex / photosynthesis | |||||||||
機能・相同性 | ![]() chloroplast photosystem II / chloroplast thylakoid / photosynthesis, light harvesting in photosystem I / photosystem II oxygen evolving complex / photosystem II assembly / oxygen evolving activity / photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor ...chloroplast photosystem II / chloroplast thylakoid / photosynthesis, light harvesting in photosystem I / photosystem II oxygen evolving complex / photosystem II assembly / oxygen evolving activity / photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / photosystem I / photosystem II / response to herbicide / extrinsic component of membrane / chlorophyll binding / photosynthetic electron transport in photosystem II / photosynthesis, light reaction / chloroplast thylakoid membrane / phosphate ion binding / : / response to light stimulus / photosynthesis / cell redox homeostasis / chloroplast / manganese ion binding / electron transfer activity / protein stabilization / iron ion binding / heme binding / calcium ion binding / metal ion binding 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.22 Å | |||||||||
![]() | Shan JY / Liu ZF | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Architecture and functional regulation of a plant PSII-LHCII megacomplex. 著者: Jianyu Shan / Dariusz M Niedzwiedzki / Rupal S Tomar / Zhenfeng Liu / Haijun Liu / ![]() ![]() 要旨: Photosystem II (PSII) splits water in oxygenic photosynthesis on Earth. The structure and function of the CSM-type PSII-LHCII (light-harvesting complex II) megacomplexes from the wild-type and PsbR- ...Photosystem II (PSII) splits water in oxygenic photosynthesis on Earth. The structure and function of the CSM-type PSII-LHCII (light-harvesting complex II) megacomplexes from the wild-type and PsbR-deletion mutant plants are studied through electron microscopy (EM), structural mass spectrometry, and ultrafast fluorescence spectroscopy [time-resolved fluorescence (TRF)]. The cryo-EM structure of a type I CSM megacomplex demonstrates that the three domains of PsbR bind to the stromal side of D1, D2, and CP43; associate with the single transmembrane helix of the redox active Cyt ; and stabilize the luminal extrinsic PsbP, respectively. This megacomplex, with PsbR and PsbY centered around the narrow interface between two dimeric PSII cores, provides the supramolecular structural basis that regulates the plastoquinone occupancy in Q site, excitation energy transfer, and oxygen evolution. PSII-LHCII megacomplexes (types I and II) and LHC aggregation levels in mutant were also interrogated and compared to wild-type plants through EM and picosecond TRF. | |||||||||
履歴 |
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構造の表示
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FSC (解像度算出) | ![]() | 15.8 KB | 表示 | ![]() |
画像 | ![]() | 80.5 KB | ||
Filedesc metadata | ![]() | 11.3 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 189.9 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 189.5 KB | 表示 | |
XML形式データ | ![]() | 581 B | 表示 | |
CIF形式データ | ![]() | 484 B | 表示 | |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.35 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : PSII-LHCII megacomplex
+超分子 #1: PSII-LHCII megacomplex
+分子 #1: Photosystem II protein D1
+分子 #2: Oxygen-evolving enhancer protein 1, chloroplastic
+分子 #3: Chlorophyll a-b binding protein, chloroplastic
+分子 #4: Chlorophyll a-b binding protein, chloroplastic
+分子 #5: Photosystem II reaction center protein T
+分子 #6: Photosystem II 5 kDa protein, chloroplastic
+分子 #7: Photosystem II reaction center W protein, chloroplastic
+分子 #8: Photosystem II reaction center X protein, chloroplastic
+分子 #9: Chlorophyll a-b binding protein, chloroplastic
+分子 #10: Photosystem II reaction center protein Z
+分子 #11: Chlorophyll a-b binding protein CP24, chloroplastic
+分子 #12: Oxygen-evolving enhancer protein 2, chloroplastic
+分子 #13: Oxygen-evolving enhancer protein 3, chloroplastic
+分子 #14: Chlorophyll a-b binding protein, chloroplastic
+分子 #15: Chlorophyll a-b binding protein, chloroplastic
+分子 #16: Photosystem II 10 kDa polypeptide, chloroplastic
+分子 #17: Photosystem II reaction center proteins PsbY, chloroplastic
+分子 #18: Photosystem II CP43 reaction center protein
+分子 #19: Photosystem II D2 protein
+分子 #20: Cytochrome b559 subunit alpha
+分子 #21: Cytochrome b559 subunit beta
+分子 #22: Photosystem II reaction center protein H
+分子 #23: Photosystem II reaction center protein I
+分子 #24: Photosystem II reaction center protein J
+分子 #25: Photosystem II reaction center protein K
+分子 #26: Photosystem II reaction center protein L
+分子 #27: Photosystem II reaction center protein M
+分子 #28: Photosystem II CP47 reaction center protein
+分子 #29: CA-MN4-O5 CLUSTER
+分子 #30: FE (II) ION
+分子 #31: CHLORIDE ION
+分子 #32: CHLOROPHYLL A
+分子 #33: PHEOPHYTIN A
+分子 #34: BETA-CAROTENE
+分子 #35: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+分子 #36: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+分子 #37: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+分子 #38: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+分子 #39: CHLOROPHYLL B
+分子 #40: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
+分子 #41: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...
+分子 #42: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...
+分子 #43: DODECYL-ALPHA-D-MALTOSIDE
+分子 #44: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+分子 #45: BICARBONATE ION
+分子 #46: PROTOPORPHYRIN IX CONTAINING FE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 6.5 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 60.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 1.5 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |