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-Structure paper
| タイトル | Architecture and functional regulation of a plant PSII-LHCII megacomplex. |
|---|---|
| ジャーナル・号・ページ | Sci Adv, Vol. 10, Issue 50, Page eadq9967, Year 2024 |
| 掲載日 | 2024年12月13日 |
著者 | Jianyu Shan / Dariusz M Niedzwiedzki / Rupal S Tomar / Zhenfeng Liu / Haijun Liu / ![]() |
| PubMed 要旨 | Photosystem II (PSII) splits water in oxygenic photosynthesis on Earth. The structure and function of the CSM-type PSII-LHCII (light-harvesting complex II) megacomplexes from the wild-type and PsbR- ...Photosystem II (PSII) splits water in oxygenic photosynthesis on Earth. The structure and function of the CSM-type PSII-LHCII (light-harvesting complex II) megacomplexes from the wild-type and PsbR-deletion mutant plants are studied through electron microscopy (EM), structural mass spectrometry, and ultrafast fluorescence spectroscopy [time-resolved fluorescence (TRF)]. The cryo-EM structure of a type I CSM megacomplex demonstrates that the three domains of PsbR bind to the stromal side of D1, D2, and CP43; associate with the single transmembrane helix of the redox active Cyt ; and stabilize the luminal extrinsic PsbP, respectively. This megacomplex, with PsbR and PsbY centered around the narrow interface between two dimeric PSII cores, provides the supramolecular structural basis that regulates the plastoquinone occupancy in Q site, excitation energy transfer, and oxygen evolution. PSII-LHCII megacomplexes (types I and II) and LHC aggregation levels in mutant were also interrogated and compared to wild-type plants through EM and picosecond TRF. |
リンク | Sci Adv / PubMed:39671473 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.22 - 4.26 Å |
| 構造データ | EMDB-39860, PDB-8z9d: ![]() EMDB-60801: The structure of C4S4M2 type PSII-LHCII megacomplex from Spinach ![]() EMDB-60802: Focus refinement of PSII core in C4S4M2 type PSII-LHCII megacomplex from spinach ![]() EMDB-60805: Focus refinement of M-24-29 subcomplex of C4S4M2 type PSII-LHCII megacomplex from Spinach ![]() EMDB-60810: Focus refinement of CS type PSII-LHCII monomer in PSII-LHCII megacomplex from Spinach |
| 化合物 | ![]() ChemComp-OEX: ![]() ChemComp-FE2: ![]() ChemComp-CL: ![]() ChemComp-CLA: ![]() ChemComp-PHO: ![]() ChemComp-BCR: ![]() ChemComp-PL9: ![]() ChemComp-LHG: ![]() ChemComp-SQD: ![]() ChemComp-LMG: ![]() ChemComp-CHL: ![]() ChemComp-LUT: ![]() ChemComp-XAT: ![]() ChemComp-NEX: ![]() ChemComp-LMU: ![]() ChemComp-DGD: ![]() ChemComp-BCT: ![]() ChemComp-HEM: |
| 由来 |
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キーワード | PHOTOSYNTHESIS / PSII / LHCII / megacomplex |
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spinacia oleracea (ホウレンソウ)
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